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460 results • Page 2 of 10
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24
votes
26
replies
2.1k
views
6 follow
Forum: Organizing a Bioinformatics Conference ?
meeting conference
updated 11 months ago by 43k • written 6.4 years ago by 161k
23
votes
15
replies
5.1k
views
6 follow
Forum: On the usage of Golang
assembly next-gen alignment
updated 11 months ago by 43k • written 5.5 years ago by ▴ 80
23
votes
12
replies
1.9k
views
6 follow
Forum: Whole-Exome Analysis on a Mainstream Desktop PC
NGS whole-exome
updated 10 months ago by 43k • written 3.9 years ago by 11k
23
votes
6
replies
13k
views
Forum: List Of File Formats Used In Bioinformatics?
bioinformatics
updated 9 months ago by 43k • written 11.5 years ago by ▴ 140
23
votes
11
replies
2.0k
views
9 follow
Forum: file and directory management best practices
file-management
updated 10 months ago by 43k • written 3.7 years ago by ▴ 140
23
votes
18
replies
1.5k
views
Forum: Versioning of Blast DB and Reproducible Research
database blast reproducible-research
updated 10 months ago by 43k • written 3.9 years ago by ▴ 60
22
votes
12
replies
1.8k
views
6 follow
Forum: What were the most impactful Bioinformatics papers published this year
papers publications
updated 10 months ago by 43k • written 4.4 years ago by ▴ 520
22
votes
11
replies
3.3k
views
Forum: ExpressionSet in the age of tidyverse
expressionset R bioconductor
updated 11 months ago by 43k • written 6.0 years ago by 13k
22
votes
8
replies
1.9k
views
10 follow
Forum: Is there any online course for bioinformatics right now or in the next two or three months?
online-course mooc
updated 10 months ago by 43k • written 4.0 years ago by ▴ 50
22
votes
15
replies
8.8k
views
11 follow
Forum: RNASeq user experience with Novogene?
RNA-Seq
updated 11 months ago by 43k • written 5.8 years ago by ▴ 80
21
votes
15
replies
2.1k
views
9 follow
Forum: Working abroad without a PhD
career
updated 10 months ago by 43k • written 4.6 years ago by ★ 1.4k
21
votes
7
replies
11k
views
Forum: Bioinformatics short paper journal resource [software/webapps/database]
journal software webapp
updated 6 months ago by ▴ 100 • written 8.1 years ago by 11k
21
votes
18
replies
1.7k
views
Forum: Is it safe to keep our data in github
github
updated 10 months ago by 43k • written 4.0 years ago by ▴ 70
21
votes
11
replies
1.9k
views
8 follow
Forum: what I want to do after the PhD?
PhD
updated 11 months ago by 43k • written 7.1 years ago by ★ 4.3k
21
votes
11
replies
2.5k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 5 months ago by 22k • written 13 months ago by ▴ 150
21
votes
11
replies
2.0k
views
Forum: Most important International Big Data Projects: Genetics and Epigenetics
big-data
updated 11 months ago by 43k • written 5.7 years ago by ★ 9.4k
21
votes
12
replies
3.5k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
3 months ago by Ram 43k
20
votes
24
replies
6.9k
views
6 follow
Forum: Python or R
RNA-Seq alignment next-gen-sequencing
updated 11 months ago by 43k • written 5.9 years ago by ▴ 160
19
votes
6
replies
3.5k
views
Forum: ngs vs microarray
microarray ngs
updated 11 months ago by 43k • written 5.8 years ago by ▴ 20
19
votes
5
replies
1.1k
views
6 follow
Forum: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics snps survey papers
4 months ago by Raony Guimarães ★ 1.4k
19
votes
7
replies
1.2k
views
9 follow
Forum: DNA in pixels
genome alignment sequencing
updated 10 months ago by 43k • written 3.4 years ago by ▴ 30
19
votes
15
replies
2.0k
views
6 follow
Forum: Cross out line in Biostars
meta
updated 11 months ago by 43k • written 5.9 years ago by 5.3k
19
votes
9
replies
3.7k
views
Forum: How to self-study (get onto) Bioinformatics and Data Analysis for Genomic, for working with real life genomics data?
R genome RNA-Seq Python
updated 11 months ago by 43k • written 5.7 years ago by ▴ 750
19
votes
14
replies
2.1k
views
Forum: What is the amount of sequencing data produced annually?
data research sequencing
updated 7 months ago by 22k • written 7 months ago by ▴ 70
19
votes
10
replies
1.5k
views
Forum: Pain points using commercial clouds
gpt tinybio
updated 7 months ago by ▴ 70 • written 7 months ago by ▴ 840
18
votes
7
replies
4.5k
views
Forum: Review of the CIGAR string format
alignment
updated 11 months ago by 43k • written 5.5 years ago by 8.5k
18
votes
2
replies
3.2k
views
Forum: Scripts on the Web: Bioinformatics Recipes
bioinformatics
updated 11 months ago by 43k • written 6.1 years ago by 100k
18
votes
11
replies
2.4k
views
8 follow
Forum: Create a File format to store Variant
hgvs json variant
updated 11 months ago by 43k • written 5.8 years ago by ★ 2.4k
18
votes
12
replies
2.5k
views
6 follow
Forum: why GATK makes things more complicated ?
genomics
updated 11 months ago by 43k • written 5.2 years ago by ▴ 280
18
votes
16
replies
1.8k
views
Forum: Why are people not working on gene prioritization and candidate gene selection for new experiment design?
gene-prioritization
updated 11 months ago by 43k • written 5.6 years ago by ▴ 20
17
votes
17
replies
2.0k
views
6 follow
Forum: Journal for beginners
journal
updated 11 months ago by 43k • written 6.1 years ago by ▴ 20
17
votes
14
replies
3.3k
views
Forum: Asking for feedback on a Python library for computing alignments
alignment Python
updated 10 months ago by ▴ 220 • written 2.3 years ago by ▴ 60
17
votes
11
replies
979
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
3 months ago by sil_bioinfo ▴ 40
17
votes
14
replies
1.9k
views
10 follow
Forum: What would be the trend in next few years in NGS era?
NGS sequencing
updated 11 months ago by 43k • written 5.4 years ago by ▴ 800
16
votes
10
replies
1.0k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 27 days ago by 5.3k • written 28 days ago by ★ 2.4k
16
votes
6
replies
8.1k
views
7 follow
Forum: What computer skills should I put on my resume?
resume genome
updated 11 months ago by 43k • written 6.4 years ago by ▴ 30
16
votes
8
replies
2.1k
views
8 follow
Forum: What are the most important unresolved problems in Bioinformatics?
bioinformatics
updated 9 months ago by 43k • written 7.6 years ago by ★ 2.5k
16
votes
7
replies
1.3k
views
Forum: What counts as "complex code"?
career
updated 11 months ago by 43k • written 5.7 years ago by ▴ 30
15
votes
9
replies
2.2k
views
6 follow
Forum: [Opinion] - What alignment software do you use?
next-gen alignment
updated 11 months ago by 43k • written 6.1 years ago by ▴ 30
15
votes
11
replies
4.7k
views
9 follow
Forum: Methodological problems are extremely common for enrichment analysis - beware the pitfalls before you publish
GSEA DAVID enrichment-analysis -geneontology
updated 11 months ago by 43k • written 2.1 years ago by ★ 1.9k
15
votes
13
replies
4.1k
views
8 follow
Forum: Does a PhD or work experience set you up better to do bioinformatics work in industry?
advice industry phd
updated 3 months ago by 3.9k • written 17 months ago by ▴ 30
15
votes
24
replies
2.6k
views
7 follow
Forum: Airport.bio: First class ticket to any biological database in the world
RNA-Seq ChIP-Seq SNP
updated 11 months ago by 43k • written 6.0 years ago by ▴ 350
15
votes
6
replies
4.3k
views
Forum: Which database software is better for bioinformatics web development? MySQL or MongoDB?
database mysql mongodb software
updated 11 months ago by 43k • written 5.6 years ago by ▴ 10
15
votes
10
replies
2.3k
views
6 follow
Forum: help identifying bioinformatics operations tools
database next-gen operations genomics
updated 11 months ago by 43k • written 5.4 years ago by ▴ 80
14
votes
5
replies
6.9k
views
Forum: Seurat vs Monocle
R RNA-Seq next-gen single-cell
updated 10 months ago by 43k • written 4.8 years ago by 3.2k
14
votes
16
replies
4.9k
views
8 follow
Forum: Yet Another Programming Language
Rust Julia
updated 11 months ago by 43k • written 6.8 years ago by 9.7k
14
votes
11
replies
6.7k
views
7 follow
Forum: Do you need a deduplication tool for FASTQ data in fastp?
deduplication fastp
updated 11 months ago by 43k • written 4.9 years ago by ★ 2.5k
14
votes
9
replies
5.0k
views
8 follow
Forum: MD into bioinformatics
MD career
updated 11 months ago by 43k • written 5.4 years ago by ▴ 30
14
votes
6
replies
2.5k
views
6 follow
Forum: Bioinformatics experience in programming
python R
updated 11 months ago by 43k • written 5.7 years ago by ▴ 20
14
votes
9
replies
2.0k
views
Forum: The push toward standardisation
stadardisation
updated 11 months ago by 43k • written 6.5 years ago by 87k
460 results • Page 2 of 10
Recent Votes
Comment: Sequence read length shorter than flow cell specification
How to calculate reliable Ka/Ks or dN/dS ratio for genes of interest from VCF file
Detecting somatic variants in non-tumor tissue without normals
Answer: Sequence read length shorter than flow cell specification
Answer: Sequence read length shorter than flow cell specification
Comment: Why gatk VariantAnnotator required bam and coverage files
A: Generate Vcf.Gz File And Its Index File Vcf.Gz.Tbi
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Recent Replies
Answer: Sequence read length shorter than flow cell specification by swbarnes2 14k
The company probably had you share your run with someone who needed the extra bases. So you get the extra bases free. Just use them unle…
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix by Ram 43k
Thank you, the `csvtk spread` is super useful. I usually import into R using `lapply` then `Reduce` using `merge` but this might be easier.
Comment: Annovar using R package by DKA ▴ 40
Thank you for your guidance. The thing is that I am unfamiliar with using such environments, unfortunately.
Comment: Treatment VS Control in Single Cell RNAseq analysis by ATpoint 82k
Open a new question, with details.
Comment: Normalize scRNAseq data to housekeeping genes to compare several datasets by ATpoint 82k
I recommend https://bioconductor.org/books/3.18/OSCA.basic/normalization.html as well as the "advanced" section in this book.
Comment: Why gatk VariantAnnotator required bam and coverage files by QX • 0
thank you!
Comment: Treatment VS Control in Single Cell RNAseq analysis by kilcdincer ▴ 10
Hello, I have more or less same experimental setting and was wondering how you proceeded with your analysis? Can I reach its GitHub reposit…
Comment: BLAST using both nucleotides and taxonomic local databases by GenoMax 141k
> I ask this as some BLAST command fields as scinames or sblastnames do not give any output with a classic nt BLAST If that information is…
Answer: BLAST using both nucleotides and taxonomic local databases by 5heikki 11k
This has been discussed [many times][1] [1]: https://www.biostars.org/p/76551/
Answer: why renaming Idents in Seurat object doesn't work? by Bioinfotec ▴ 10
I think when you give indent to seuObj : ```r Idents(seuObj) <- 'RNA_snn_res.0.1' ``` You may wrongly assign other value to it such as `…
Answer: ScRNAseq-How to correctly choose cell type marker genes by Bioinfotec ▴ 10
In fact, there is no gold standard for cell type annotation. To achieve correct cell type classification, you can combine the two methos. F…
Answer: ScRNA data question by Bioinfotec ▴ 10
In violin plot, a dot represents a cell. Actually, the straight lines you see in your infected image just means these cells have the same e…
Comment: Sequence read length shorter than flow cell specification by GenoMax 141k
> the have sent data with different sequencing read lengths: 55bp pair ended and 80bp single ended If they sent data that is not what you…
Comment: How to know synonymous and non-synonymous SNPs from transcriptome sequencing by rohitsatyam102 ▴ 850
I have some off-the-chart dN/dS values for few genes (>20) when using your script on VCF obtained from a 20K plasmodium genomes (pf7K). | …
Answer: Sequence read length shorter than flow cell specification by ATpoint 82k
It is the amount of reagent in the kit that determines read length. Sometimes index cycle reagents are allocated to R1/R2 or they used anot…
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