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457 results • Page 2 of 10
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24
votes
26
replies
2.1k
views
6 follow
Forum: Organizing a Bioinformatics Conference ?
meeting conference
updated 11 months ago by 43k • written 6.4 years ago by 161k
23
votes
6
replies
13k
views
Forum: List Of File Formats Used In Bioinformatics?
bioinformatics
updated 9 months ago by 43k • written 11.5 years ago by ▴ 140
23
votes
18
replies
1.5k
views
Forum: Versioning of Blast DB and Reproducible Research
database blast reproducible-research
updated 10 months ago by 43k • written 3.9 years ago by ▴ 50
23
votes
15
replies
5.0k
views
6 follow
Forum: On the usage of Golang
assembly next-gen alignment
updated 10 months ago by 43k • written 5.5 years ago by ▴ 80
23
votes
11
replies
2.0k
views
9 follow
Forum: file and directory management best practices
file-management
updated 10 months ago by 43k • written 3.7 years ago by ▴ 140
23
votes
12
replies
1.9k
views
6 follow
Forum: Whole-Exome Analysis on a Mainstream Desktop PC
NGS whole-exome
updated 10 months ago by 43k • written 3.8 years ago by 11k
22
votes
12
replies
1.8k
views
6 follow
Forum: What were the most impactful Bioinformatics papers published this year
papers publications
updated 10 months ago by 43k • written 4.3 years ago by ▴ 520
22
votes
8
replies
1.9k
views
10 follow
Forum: Is there any online course for bioinformatics right now or in the next two or three months?
online-course mooc
updated 10 months ago by 43k • written 4.0 years ago by ▴ 50
22
votes
15
replies
8.8k
views
11 follow
Forum: RNASeq user experience with Novogene?
RNA-Seq
updated 10 months ago by 43k • written 5.8 years ago by ▴ 80
22
votes
11
replies
3.3k
views
Forum: ExpressionSet in the age of tidyverse
expressionset R bioconductor
updated 10 months ago by 43k • written 5.9 years ago by 13k
21
votes
18
replies
1.7k
views
Forum: Is it safe to keep our data in github
github
updated 10 months ago by 43k • written 3.9 years ago by ▴ 70
21
votes
15
replies
2.1k
views
9 follow
Forum: Working abroad without a PhD
career
updated 10 months ago by 43k • written 4.6 years ago by ★ 1.4k
21
votes
11
replies
1.9k
views
8 follow
Forum: what I want to do after the PhD?
PhD
updated 11 months ago by 43k • written 7.1 years ago by ★ 4.3k
21
votes
7
replies
11k
views
Forum: Bioinformatics short paper journal resource [software/webapps/database]
journal software webapp
updated 6 months ago by ▴ 100 • written 8.0 years ago by 11k
21
votes
11
replies
2.0k
views
Forum: Most important International Big Data Projects: Genetics and Epigenetics
big-data
updated 10 months ago by 43k • written 5.7 years ago by ★ 9.4k
21
votes
11
replies
2.4k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 4 months ago by 22k • written 13 months ago by ▴ 150
21
votes
12
replies
3.5k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
3 months ago by Ram 43k
20
votes
24
replies
6.9k
views
6 follow
Forum: Python or R
RNA-Seq alignment next-gen-sequencing
updated 10 months ago by 43k • written 5.9 years ago by ▴ 160
19
votes
7
replies
1.2k
views
9 follow
Forum: DNA in pixels
genome alignment sequencing
updated 10 months ago by 43k • written 3.4 years ago by ▴ 30
19
votes
14
replies
2.0k
views
7 follow
Forum: What's the general view on point & click bioinformatics for routine analyses?
ngs RNA-Seq next-gen-sequencing
11 months ago by Simon ▴ 40
19
votes
6
replies
3.4k
views
Forum: ngs vs microarray
microarray ngs
updated 10 months ago by 43k • written 5.8 years ago by ▴ 20
19
votes
14
replies
2.0k
views
Forum: What is the amount of sequencing data produced annually?
data research sequencing
updated 6 months ago by 22k • written 6 months ago by ▴ 70
19
votes
15
replies
2.0k
views
6 follow
Forum: Cross out line in Biostars
meta
updated 10 months ago by 43k • written 5.9 years ago by 5.3k
19
votes
9
replies
3.6k
views
Forum: How to self-study (get onto) Bioinformatics and Data Analysis for Genomic, for working with real life genomics data?
R genome RNA-Seq Python
updated 10 months ago by 43k • written 5.7 years ago by ▴ 750
19
votes
5
replies
1.1k
views
6 follow
Forum: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics snps survey papers
3 months ago by Raony Guimarães ★ 1.4k
19
votes
10
replies
1.5k
views
Forum: Pain points using commercial clouds
gpt tinybio
updated 7 months ago by ▴ 70 • written 7 months ago by ▴ 830
18
votes
12
replies
2.5k
views
6 follow
Forum: why GATK makes things more complicated ?
genomics
updated 10 months ago by 43k • written 5.2 years ago by ▴ 280
18
votes
2
replies
3.2k
views
Forum: Scripts on the Web: Bioinformatics Recipes
bioinformatics
updated 10 months ago by 43k • written 6.1 years ago by 100k
18
votes
16
replies
1.8k
views
Forum: Why are people not working on gene prioritization and candidate gene selection for new experiment design?
gene-prioritization
updated 10 months ago by 43k • written 5.6 years ago by ▴ 20
18
votes
11
replies
2.4k
views
8 follow
Forum: Create a File format to store Variant
hgvs json variant
updated 10 months ago by 43k • written 5.8 years ago by ★ 2.4k
18
votes
7
replies
4.4k
views
Forum: Review of the CIGAR string format
alignment
updated 10 months ago by 43k • written 5.5 years ago by 8.5k
17
votes
17
replies
1.9k
views
6 follow
Forum: Journal for beginners
journal
updated 10 months ago by 43k • written 6.1 years ago by ▴ 20
17
votes
14
replies
1.9k
views
10 follow
Forum: What would be the trend in next few years in NGS era?
NGS sequencing
updated 10 months ago by 43k • written 5.4 years ago by ▴ 800
17
votes
14
replies
3.2k
views
Forum: Asking for feedback on a Python library for computing alignments
alignment Python
updated 10 months ago by ▴ 220 • written 2.2 years ago by ▴ 60
17
votes
11
replies
949
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
12 weeks ago by sil_bioinfo ▴ 40
16
votes
8
replies
2.1k
views
8 follow
Forum: What are the most important unresolved problems in Bioinformatics?
bioinformatics
updated 9 months ago by 43k • written 7.5 years ago by ★ 2.5k
16
votes
7
replies
1.3k
views
Forum: What counts as "complex code"?
career
updated 10 months ago by 43k • written 5.6 years ago by ▴ 30
16
votes
10
replies
998
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 17 days ago by 5.3k • written 18 days ago by ★ 2.4k
16
votes
6
replies
8.0k
views
7 follow
Forum: What computer skills should I put on my resume?
resume genome
updated 11 months ago by 43k • written 6.3 years ago by ▴ 30
15
votes
10
replies
2.3k
views
6 follow
Forum: help identifying bioinformatics operations tools
database next-gen operations genomics
updated 10 months ago by 43k • written 5.4 years ago by ▴ 80
15
votes
9
replies
2.1k
views
6 follow
Forum: [Opinion] - What alignment software do you use?
next-gen alignment
updated 10 months ago by 43k • written 6.0 years ago by ▴ 30
15
votes
6
replies
4.2k
views
Forum: Which database software is better for bioinformatics web development? MySQL or MongoDB?
database mysql mongodb software
updated 10 months ago by 43k • written 5.6 years ago by ▴ 10
15
votes
11
replies
4.6k
views
9 follow
Forum: Methodological problems are extremely common for enrichment analysis - beware the pitfalls before you publish
GSEA DAVID enrichment-analysis -geneontology
updated 11 months ago by 43k • written 2.1 years ago by ★ 1.9k
15
votes
24
replies
2.5k
views
7 follow
Forum: Airport.bio: First class ticket to any biological database in the world
RNA-Seq ChIP-Seq SNP
updated 10 months ago by 43k • written 6.0 years ago by ▴ 350
15
votes
13
replies
4.0k
views
8 follow
Forum: Does a PhD or work experience set you up better to do bioinformatics work in industry?
advice industry phd
updated 3 months ago by 3.9k • written 17 months ago by ▴ 30
14
votes
9
replies
1.9k
views
Forum: The push toward standardisation
stadardisation
updated 11 months ago by 43k • written 6.4 years ago by 87k
14
votes
5
replies
6.9k
views
Forum: Seurat vs Monocle
R RNA-Seq next-gen single-cell
updated 10 months ago by 43k • written 4.8 years ago by 3.2k
14
votes
10
replies
2.1k
views
Forum: WGBS alignment with BitMapperBS
BitMapperBS bismark
updated 10 months ago by 43k • written 4.8 years ago by ★ 9.4k
14
votes
11
replies
6.7k
views
7 follow
Forum: Do you need a deduplication tool for FASTQ data in fastp?
deduplication fastp
updated 10 months ago by 43k • written 4.9 years ago by ★ 2.5k
14
votes
9
replies
4.9k
views
8 follow
Forum: MD into bioinformatics
MD career
updated 10 months ago by 43k • written 5.3 years ago by ▴ 30
457 results • Page 2 of 10
Recent Votes
Comment: Where to find the homopolymer regions bed file for Hg002 genome?
Visualization of PROKKA Annotation Result?
A: snakemake output directory
Answer: I'm new to cell ranger. Can single cells prepared with a protocol other than the
Answer: I am new to single cell analysis. Can RNA velocity (spliced/unspliced) be calcul
Answer: I am new to single cell analysis. Can RNA velocity (spliced/unspliced) be calcul
A: What does "gappedPeak" mean
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Recent Replies
Comment: Tumour purity and ploidy estimation by bp22 ▴ 60
Hi Llyod98, This issue is still unresolved and I have not been able to find a tool or solution for purity and ploidy estimation when ther…
Comment: Ensembl gene id conversion by ATpoint 81k
Can you add some examples. It is hard to debug textual descriptions.
Answer: Which experiments generate Position Frequency Matrix? by ATpoint 81k
Yes, ChIP-seq or similar methods that assay the DNA binding of a protein. But for a reliable matrix you don't need one or two but many data…
Comment: Statistical Advice Needed for RNAseq Data Analysis by ATpoint 81k
> ensuring it's not merely a product of random chance If your approach of getting DEGs is a proper and sound one (e.g. DESeq2 or limma) th…
Comment: Can I compare kallisto counts from samples with different amount of reads? by ATpoint 81k
Unequal numbers of reads are normal, that is why normalization is needed. If you want to do simple analysis such as number of detected gene…
Comment: Error in edgeR/Deseq2 Analysis by ATpoint 81k
Please ask at https://help.galaxyproject.org/ which is the Galaxy help forum.
Comment: RNAseq 1 control 2 different treatment by matteo.levorato • 0
yes, this is what I did. However, since my treatments are very similar each other, when I check upregulated genes in one treatment, they ar…
Comment: snakemake output directory by 进 • 0
it is right answer
Comment: I am new to single cell analysis. Can RNA velocity (spliced/unspliced) be calcul by Spring • 0
Thank you for the kind guidance!
Answer: I am new to single cell analysis. Can RNA velocity (spliced/unspliced) be calcul by ATpoint 81k
No, you either have the spliced and unspliced counts available that you need, or you need the fastq files to generate them. Before you dive…
Comment: Convert GVF to VCF by george-hall-ucl • 0
This has moved - it is now at: [https://github.com/Ensembl/ensembl-variation/blob/main/scripts/misc/release/gvf2vcf.pl][1]. [1]: https:…
Comment: I'm new to cell ranger. Can single cells prepared with a protocol other than the by Spring • 0
Thank you very much!
Comment: I'm new to cell ranger. Can single cells prepared with a protocol other than the by Spring • 0
Thank you so much! If the 10x Chromium protocol is not used, should the processing be done with HISAT2, FeatureCounts, and so on?
Comment: I'm new to cell ranger. Can single cells prepared with a protocol other than the by fracarb8 ★ 1.6k
For the paper, they used the `indrop` pipeline (https://github.com/indrops/indrops), and you should have a look at it, and try to run with …
Answer: I'm new to cell ranger. Can single cells prepared with a protocol other than the by ATpoint 81k
CellRanger is specific for 10x Chromium. I did not check the details of this single-cell platform so I recommend to see what papers that us…
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