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1,000 results • Page
2 of 20
Sort: Votes
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Votes
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70
votes
19
replies
5.6k
views
13 follow
Forum:
Thrown Into A Bioinformatics Position
computational-analysis
updated 21 months ago by
Ram
43k • written 8.5 years ago by
System
▴ 170
69
votes
39
replies
4.6k
views
14 follow
Forum:
Preparation for Bioinformatics.SE closing
meta
biostars
updated 11 months ago by
Ram
43k • written 6.9 years ago by
John
13k
68
votes
22
replies
7.8k
views
16 follow
Forum:
Learning Python: list of resources
python
updated 12 months ago by
Ram
43k • written 7.5 years ago by
dago
★ 2.8k
68
votes
48
replies
8.7k
views
16 follow
Forum:
Stranger Things: unexpected limitations of popular tools
samtools
hisat2
updated 11 months ago by
Ram
43k • written 6.8 years ago by
Istvan Albert
100k
67
votes
40
replies
9.7k
views
12 follow
Forum:
I want to re-open the old debate: python or perl ?
perl
python
updated 2.2 years ago by
Ram
43k • written 9.4 years ago by
Gabriel R.
★ 2.9k
67
votes
21
replies
6.1k
views
8 follow
Forum:
Meta: Markdown in Biostars (now enabled)
meta
13 months ago by
Ram
43k
66
votes
29
replies
16k
views
15 follow
Forum:
Fundamentals Of Designing Biological Databases?
database
updated 14 months ago by
Ram
43k • written 13.5 years ago by
anon1234z
▴ 130
66
votes
21
replies
6.8k
views
18 follow
Forum:
Is there anyone who was a pure biologist?
next-gen
updated 13 months ago by
Ram
43k • written 10.0 years ago by
Chong Tang
▴ 100
65
votes
57
replies
8.2k
views
12 follow
Forum:
Bioinformatics Mobile App
android
genome
iphone
mutations
updated 2.2 years ago by
Ram
43k • written 9.5 years ago by
aeinsights
▴ 20
65
votes
26
replies
19k
views
13 follow
Forum:
Macosx For Bioinformatics?
linux
updated 14 months ago by
Ram
43k • written 10.4 years ago by
Eric Normandeau
11k
65
votes
27
replies
7.4k
views
14 follow
Forum:
What is the reason for most software errors in Bioinformatics according to you?
software-error
blast
sequencing
SNP
R
updated 12 months ago by
Ram
43k • written 8.1 years ago by
harne.priyanka
▴ 110
64
votes
8
replies
8.2k
views
7 follow
Forum:
Isaac Asimov Accurately Predicts Woes Of Bioinformatician
bioinformatician
updated 14 months ago by
Ram
43k • written 11.0 years ago by
Obi Griffith
20k
64
votes
24
replies
3.0k
views
12 follow
Forum:
How much I must feel useless?
machine-learning
Programming
statistics
updated 11 months ago by
Ram
43k • written 5.6 years ago by
Molecular_genetics
▴ 80
63
votes
11
replies
15k
views
9 follow
Forum:
Web Based Interactive Resources To Learn Programming And/Or Bioinformatics
programming
updated 14 months ago by
Ram
43k • written 10.8 years ago by
Malachi Griffith
19k
63
votes
9
replies
3.9k
views
Forum:
Should The "Post Closing" Option Exist? Vote Now!
meta
updated 14 months ago by
Ram
43k • written 10.9 years ago by
Istvan Albert
100k
63
votes
28
replies
4.0k
views
11 follow
Forum:
Bioinformatics analyses & errors: need for better post-publication tools?
next-gen
RNA-Seq
updated 12 months ago by
Ram
43k • written 8.0 years ago by
ablanchetcohen
★ 1.2k
61
votes
16
replies
8.0k
views
14 follow
Forum:
Rust or C++, what to learn after Go for high-performance bioinformatics tools?
library
updated 15 months ago by
Samuel Lampa
★ 1.3k • written 3.5 years ago by
shenwei356
8.4k
61
votes
70
replies
14k
views
21 follow
Forum:
Biostar 2.0 released
meta
biostars
updated 14 months ago by
Ram
43k • written 10.0 years ago by
Istvan Albert
100k
60
votes
14
replies
12k
views
16 follow
Forum:
Interesting bioinformatics blogs (2017 edition)
blogs
updated 14 months ago by
Ram
43k • written 7.1 years ago by
WouterDeCoster
47k
60
votes
28
replies
8.3k
views
27 follow
Forum:
Jim Robinson of the Integrative Genomics Viewer (IGV) uses this
uses-this
updated 13 months ago by
Ram
43k • written 9.6 years ago by
Istvan Albert
100k
60
votes
25
replies
5.5k
views
8 follow
Forum:
Am I crazy, or are most published RNA-seq studies vastly underpowered?
sample-size
statistical-power
RNA-Seq
updated 10 months ago by
Ram
43k • written 3.7 years ago by
telroyjatter
▴ 230
57
votes
9
replies
5.7k
views
14 follow
Forum:
Handbooks for Bioinformatics Ninjas
gallery
updated 12 months ago by
Ram
43k • written 7.9 years ago by
Shicheng Guo
★ 9.4k
57
votes
15
replies
6.2k
views
9 follow
Forum:
Good practices while dealing with very large datasets
Big-Data
Programming
updated 11 months ago by
Ram
43k • written 7.0 years ago by
VHahaut
★ 1.2k
57
votes
42
replies
6.6k
views
12 follow
Forum:
Why is academic software hard to install?
software-installation
updated 2.2 years ago by
Ram
43k • written 9.5 years ago by
lh3
33k
57
votes
43
replies
16k
views
8 follow
Forum:
Best RNA-Seq aligner: A comparison of mapping tools
NGS
alignment
genome
RNA-Seq
sequence
updated 13 months ago by
Ram
43k • written 5.3 years ago by
David Langenberger
11k
57
votes
15
replies
4.8k
views
9 follow
Forum:
Entice Biologists To Learn R
r
updated 14 months ago by
Ram
43k • written 12.6 years ago by
Madelaine Gogol
5.3k
57
votes
19
replies
28k
views
6 follow
Forum:
Attention: Bowtie2 And Multiple Hits
next-gen
bowtie
mapping
updated 14 months ago by
Ram
43k • written 11.5 years ago by
David Langenberger
11k
56
votes
19
replies
4.1k
views
9 follow
Forum:
How to apply for a job
resume
cover-letter
application
CV
updated 11 months ago by
Ram
43k • written 6.7 years ago by
Emily
23k
55
votes
16
replies
6.0k
views
8 follow
Forum:
Mission Impossible: you have 1 minute to analyze the Ebola Genome
ebola
mission-impossible
updated 19 months ago by
Ram
43k • written 9.5 years ago by
Istvan Albert
100k
54
votes
34
replies
4.9k
views
13 follow
Forum:
Should We Release Database Dumps Of All Questions On Biostar?
meta
biostars
updated 14 months ago by
Ram
43k • written 13.4 years ago by
Istvan Albert
100k
54
votes
16
replies
5.7k
views
13 follow
Forum:
The new era of bioinformatics: simple and fast tools, fewer and more informative data
kallisto
computational-cost
updated 15 months ago by
Ram
43k • written 9.0 years ago by
Istvan Albert
100k
52
votes
8
replies
4.8k
views
8 follow
Forum:
What Is The Proper Way To Think About Reinventing The Wheel As A Bioinformatician?
software-development
updated 14 months ago by
Ram
43k • written 11.2 years ago by
KCC
★ 4.1k
52
votes
18
replies
6.3k
views
14 follow
Forum:
Do You Feel Good Or Bad About Putting Your Photo At Biostar?
meta
biostars
updated 14 months ago by
Ram
43k • written 10.6 years ago by
ugly.betty77
★ 1.1k
51
votes
24
replies
9.1k
views
7 follow
Forum:
Bio-informatics salary distribution versus Java salary distribution in the UK
career
updated 2.4 years ago by
Ram
43k • written 9.7 years ago by
William
★ 5.3k
51
votes
16
replies
5.8k
views
11 follow
Forum:
What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2011?
papers
updated 14 months ago by
Ram
43k • written 12.4 years ago by
Farhat
★ 2.9k
50
votes
20
replies
6.0k
views
18 follow
Forum:
Explain bioinformatics to a 10 year old
bioinformatics
updated 13 months ago by
Ram
43k • written 8.1 years ago by
novice
★ 1.1k
50
votes
8
replies
9.5k
views
6 follow
Forum:
Encode Commentary From Dan Graur
encode
updated 14 months ago by
Ram
43k • written 11.2 years ago by
Damian Kao
16k
50
votes
4
replies
9.3k
views
Forum:
How to add images to a Biostars post
how-to
14 months ago by
Ram
43k
50
votes
19
replies
4.9k
views
7 follow
Forum:
Proposal: Biostar Wants To Run Your Ads. Feedback Requested.
biostars
updated 14 months ago by
Ram
43k • written 11.2 years ago by
Istvan Albert
100k
49
votes
19
replies
7.3k
views
13 follow
Forum:
Why Are There No Proteomics Questions In Biostar?
proteomics
biostars
updated 14 months ago by
Ram
43k • written 13.5 years ago by
Jdnavarro
▴ 410
49
votes
20
replies
2.0k
views
10 follow
Forum:
First experiences matter
first-time
tolerance
updated 3 months ago by
Michael Love
★ 2.6k • written 3 months ago by
Mensur Dlakic
★ 27k
49
votes
36
replies
8.6k
views
11 follow
Forum:
New features and bug fixes for Biostar to work on during Google Summer of Code
meta
biostars
gsoc
updated 14 months ago by
Ram
43k • written 10.0 years ago by
Paolo
▴ 110
49
votes
27
replies
7.3k
views
10 follow
Forum:
Possible augmented reality tools for bioinformatics and current problems
visualization
3D
augmented-reality
updated 13 months ago by
Ram
43k • written 8.5 years ago by
Peter Leontev
▴ 90
48
votes
38
replies
17k
views
8 follow
Forum:
Eukaryotic Genome Annotation in 2016
software
genome
annotation
updated 12 months ago by
Ram
43k • written 8.1 years ago by
Eric Normandeau
11k
47
votes
24
replies
6.5k
views
15 follow
Forum:
What's fascinating about Bioinformatics for potential students?
career
msc
updated 16 months ago by
Ram
43k • written 6.5 years ago by
Michael
54k
47
votes
37
replies
8.7k
views
12 follow
Forum:
The Organisation Of Really Contrived Acronyms (Orca)
acronyms
updated 14 months ago by
Ram
43k • written 10.7 years ago by
Niallhaslam
2.3k
47
votes
30
replies
4.3k
views
10 follow
Forum:
Bioinformatics software distribution
software
updated 11 months ago by
Ram
43k • written 7.1 years ago by
Petr Ponomarenko
★ 2.8k
47
votes
7
replies
3.9k
views
7 follow
Forum:
Would There Be Interest In A 'Next-Gen Sequencing' Stackexchange Site?
meta
next-gen
updated 14 months ago by
Ram
43k • written 11.4 years ago by
14134125465346445
★ 3.6k
47
votes
11
replies
4.1k
views
Forum:
What Operating System Are You Using For Bioinformatic Work?
linux
windows
updated 14 months ago by
Ram
43k • written 10.4 years ago by
Eric Normandeau
11k
47
votes
14
replies
5.1k
views
Forum:
Was FASTA ever popular?
FASTA
alignment
updated 12 months ago by
Ram
43k • written 7.6 years ago by
John
13k
1,000 results • Page
2 of 20
Recent Votes
Comment: clustalo - align milions of short seq, memory issue
Comment: downloading genomes in fasta format from accession ids
Comment: PCA plot (Suggestions Needed)
Answer: PCA plot (Suggestions Needed)
PCA plot (Suggestions Needed)
Comment: To get p-values for the TPM
A: Where To Download Pam50 Gene Set?
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Recent Replies
Answer: clustalo - align milions of short seq, memory issue
by
emmanouil.a
▴ 120
Hi GenoMax and Mensur Dlakic, thank you for your feedback. I'm changing approach in the way to work with a smaller number of seq.
Comment: Annovar using R package
by
Pierre Lindenbaum
161k
duplicate of your previous question: https://www.biostars.org/p/9592646/ why using R when there are shell-based tools like VEP ? slivar , …
Comment: Trouble with Pilon installation
by
Lissa Cruz Saavedra
• 0
Hi I am trying to install the software but I didn't find the way to clone from github. Could you help me with the pipeline of instruction…
Comment: Adding CB tag to bam file
by
swbarnes2
14k
I think both these programs are just adding the same tag to every line. I don't think either cares whether it's called RG or CB
Comment: PCA plot (Suggestions Needed)
by
swbarnes2
14k
Even if this was real data, the tiny numbers for the PC1 and PC2 indicate that the differences between the samples are all absolutely tiny.…
Comment: Creating Synthetic Sequences for a ML Model
by
biochugs
• 0
Thanks for the input. I'll continue to think of more ideas that may be achievable.
Comment: PCA plot (Suggestions Needed)
by
Jeremy
▴ 890
You could also try kernel PCA, which is a non-linear form of PCA, although this will be more time-consuming.
Comment: Troubleshooting RNA-seq data with DNA contamination
by
DKA
▴ 40
I have unstranded RNA-seq data. Please share the tool with me, if you do not mind.
Comment: PCA plot (Suggestions Needed)
by
Mensur Dlakic
★ 27k
Everything that @f1f58aed said, plus that there may be no data manipulation that will give you the clusters you expected. Sometimes there a…
Answer: Creating Synthetic Sequences for a ML Model
by
Mensur Dlakic
★ 27k
It is a good idea, though I don't see how it would work in practice. I think you will encounter at least one additional problem on top of …
Answer: PCA plot (Suggestions Needed)
by
Jeremy
▴ 890
Right now, the WT samples are clustering together, but you have one mutant sample off by itself, which could be an outlier. How did you sc…
Comment: Adding CB tag to bam file
by
GenoMax
141k
@yokofakun had written this: https://www.biostars.org/p/336965/ https://github.com/ruqianl/appendCB may also be useful.
Comment: Troubleshooting RNA-seq data with DNA contamination
by
GenoMax
141k
Please post information about your tool as a `tools` category post. Don't wait/ask other users to ask you.
Comment: Adding CB tag to bam file
by
Maria
• 0
Sorry for the delay in my answer, I got sidetracked. I am using ```samtools addreplacerg -r "@RG\tID:$cell" -o $cell.bam -h $LINE``` bu…
Answer: Troubleshooting RNA-seq data with DNA contamination
by
haibol2017
▴ 10
We just developed a R package to tackle this problem. Do you have stranded or unstranded RNA-seq data contaminated with gDNA? If you wou…
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