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1,000 results • Page 3 of 20
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47
votes
14
replies
5.1k
views
Forum: Was FASTA ever popular?
FASTA alignment
updated 12 months ago by 43k • written 7.5 years ago by 13k
46
votes
12
replies
36k
views
6 follow
Forum: BWA vs Bowtie 2 (Poll)
BWA Poll Bowtie2
updated 23 months ago by 43k • written 9.1 years ago by ▴ 270
46
votes
9
replies
8.5k
views
10 follow
Forum: Bioinformatics For Clinicians - What To Teach Them?
education
updated 13 months ago by 43k • written 10.3 years ago by ▴ 140
45
votes
19
replies
2.8k
views
9 follow
Forum: Where and how NGS techniques are heading for the next 5 years?
next-gen NGS
updated 11 months ago by 43k • written 6.9 years ago by 5.8k
45
votes
35
replies
6.1k
views
12 follow
Forum: looking for a bioinformatics project to do during military rotation
military
updated 22 months ago by 43k • written 6.6 years ago by ▴ 60
45
votes
23
replies
6.3k
views
6 follow
Forum: biostar.slack.com: Chat for the biostars community -- [ feel free to join ]
chat slack
updated 14 months ago by 43k • written 6.1 years ago by 104k
44
votes
11
replies
5.2k
views
7 follow
Forum: Asking The Developers Before Asking Biostars
meta
updated 13 months ago by 43k • written 10.6 years ago by ▴ 860
44
votes
11
replies
2.8k
views
6 follow
Forum: Why can't we downvote on this forum?
meta biostars
updated 12 months ago by 43k • written 7.4 years ago by ▴ 740
43
votes
37
replies
2.5k
views
7 follow
Forum: Checking the quality and reliability in bioinformatics
reproducibility
updated 11 months ago by 43k • written 6.5 years ago by ★ 4.3k
43
votes
16
replies
4.6k
views
10 follow
Forum: Need Advice On How To Become A Senior Bioinformatician
advice career
updated 13 months ago by 43k • written 10.9 years ago by ▴ 30
42
votes
17
replies
38k
views
15 follow
Forum: List Of Genome Sequencing Centers/Institutes
genomics institutes
updated 13 months ago by 43k • written 11.2 years ago by 19k
42
votes
21
replies
15k
views
10 follow
Forum: A Database Of Signatures Of Selection In The 1000 Genomes Dataset
1000genomes human selection
updated 13 months ago by 43k • written 10.1 years ago by 28k
42
votes
20
replies
3.4k
views
11 follow
Forum: What are the most popular NGS tools?
ensembl NGS
updated 12 months ago by 43k • written 8.0 years ago by 23k
42
votes
8
replies
4.7k
views
13 follow
Forum: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2017?
sequencing survey SNP
updated 14 months ago by 43k • written 6.3 years ago by ★ 2.8k
42
votes
15
replies
7.7k
views
8 follow
Forum: Directions In Bioinformatics And Web-Based Data Visualization: Revisiting Javascript?
data-visualization python r
updated 13 months ago by 43k • written 11.4 years ago by 5.8k
41
votes
27
replies
5.9k
views
8 follow
Forum: Why bioinformaticians need to know programming languages?
Python R Programming
updated 11 months ago by 43k • written 6.9 years ago by ▴ 90
40
votes
11
replies
7.0k
views
9 follow
Forum: "An Explosion Of Bioinformatics Careers" - from Science Careers
career
updated 13 months ago by 43k • written 9.8 years ago by 3.2k
40
votes
11
replies
4.8k
views
6 follow
Forum: what is next going to happen in bioinformatics?
current-research
updated 23 months ago by 43k • written 9.1 years ago by ▴ 920
40
votes
23
replies
4.1k
views
12 follow
Forum: Why learn programming in bioinformatics?
Programming
updated 10 months ago by 43k • written 5.6 years ago by ▴ 110
40
votes
24
replies
3.8k
views
13 follow
Forum: What programming language do you use the most in your work? Which one do you like the best?
Programming Python R Scripting
updated 13 months ago by 43k • written 3.2 years ago by ▴ 100
39
votes
25
replies
4.4k
views
9 follow
Forum: Making a mac book Bioinfo ready (El Capitan)
mac software-installation
updated 12 months ago by 43k • written 8.1 years ago by 11k
39
votes
21
replies
13k
views
10 follow
Forum: Orientation of PE reads a review of --fr --ff and --rf meanings
RNA-Seq
updated 10 months ago by 43k • written 5.5 years ago by 8.5k
38
votes
18
replies
4.2k
views
11 follow
Forum: Should We Have A Biostar Conference Or Meeting?
conference biostars
updated 14 months ago by 43k • written 10.8 years ago by 54k
38
votes
17
replies
3.2k
views
7 follow
Forum: Why does bioinformatics use a special format like a BAM file instead of a database like SQLite?
sam bam samtools
updated 10 months ago by 43k • written 3.7 years ago by ▴ 250
38
votes
31
replies
2.8k
views
10 follow
Forum: To the Biologists out there: What bioinf tool are you missing?
education career
updated 12 months ago by 43k • written 7.7 years ago by ▴ 180
38
votes
19
replies
10k
views
10 follow
Forum: Kallisto New RNA-seq quantification method discussion
RNA-Seq
updated 12 months ago by 43k • written 8.0 years ago by ▴ 550
37
votes
30
replies
5.0k
views
7 follow
Forum: Spam In Rss Feeds
meta RSS
updated 13 months ago by 43k • written 11.2 years ago by 5.3k
37
votes
13
replies
3.5k
views
6 follow
Forum: Bringing Back Downvote
meta
updated 13 months ago by 43k • written 10.9 years ago by 33k
37
votes
16
replies
4.0k
views
9 follow
Forum: Sanger sequencing is no longer the gold standard?
Sanger NGS
updated 11 months ago by 43k • written 6.5 years ago by ▴ 220
36
votes
7
replies
2.9k
views
7 follow
Forum: What are benefits of Pursuing Bioinformatics over Software Engineering?
career
updated 21 months ago by 43k • written 8.2 years ago by ▴ 10
36
votes
18
replies
8.1k
views
Forum: How Does Genomebrowse Free Genome Browser From Golden Helix Compare?
visualization genome-browser
updated 3.0 years ago by 20 • written 11.1 years ago by ▴ 140
36
votes
2
replies
6.1k
views
Forum: Brian Haas of the Trinity Assembler uses this
uses-this
updated 13 months ago by 43k • written 9.5 years ago by 100k
36
votes
39
replies
4.1k
views
11 follow
Forum: Survey/Vote: If you could double the speed of any three commandline tools, which three would they be?
blast RNA-Seq alignment next-gen-sequencing
updated 11 months ago by 43k • written 6.4 years ago by ▴ 130
36
votes
34
replies
2.8k
views
9 follow
Forum: New to bioinformatics.....
gene
updated 11 months ago by 43k • written 6.1 years ago by • 0
36
votes
21
replies
19k
views
9 follow
Forum: What Is Your Favorite Conserved Protein?
protein conservation
updated 13 months ago by 43k • written 10.7 years ago by 11k
36
votes
8
replies
3.6k
views
6 follow
Forum: Time Management Strategies and Advice for Bioinformaticians
professional-skills
updated 12 months ago by 43k • written 7.9 years ago by ▴ 880
35
votes
21
replies
3.0k
views
11 follow
Forum: Should you restrict use of your software based on your political views?
software
updated 19 months ago by 43k • written 8.6 years ago by 27k
35
votes
19
replies
3.7k
views
6 follow
Forum: For the general public seeking advice from this community
diagnosis rare-disease
updated 19 months ago by 43k • written 8.6 years ago by 11k
35
votes
19
replies
4.0k
views
Forum: Crowd Sourcing The 'Analysis' Of The 'Big Foot' Genome
bigfoot genome
updated 13 months ago by 43k • written 11.2 years ago by 4.5k
34
votes
19
replies
7.9k
views
12 follow
Forum: Programming Language In Bioinformatics
perl python
updated 13 months ago by 43k • written 11.0 years ago by ▴ 260
34
votes
11
replies
9.3k
views
13 follow
Forum: Gallery: Computational tools for DNA methylation
computational-tools methylation
updated 20 months ago by 43k • written 8.5 years ago by ★ 9.4k
34
votes
8
replies
2.7k
views
9 follow
Forum: Bioinformatician, a jack of all trades, but in the eye of the CV-beholder a master of none? How can your CV reflect the diverse skills that you have …
cv industry career
updated 10 months ago by 43k • written 4.5 years ago by ▴ 300
34
votes
23
replies
5.3k
views
8 follow
Forum: Unbelievably rapid NGS analysis by Churchill
sequencing NGS churchill
2.0 years ago by Ram 43k
34
votes
12
replies
3.7k
views
11 follow
Forum: How To Change An Expertise In Bioinformatics
career
updated 14 months ago by 43k • written 13.0 years ago by ▴ 220
34
votes
12
replies
11k
views
9 follow
Forum: Is going back to the wet lab worth it?
careerwet-lab
updated 12 months ago by 43k • written 7.3 years ago by ▴ 810
34
votes
13
replies
6.1k
views
11 follow
Forum: Best Way To Learn Bioinformatics For System Level Programmers.
NGS HPC
updated 13 months ago by 43k • written 10.2 years ago by ▴ 90
34
votes
22
replies
2.9k
views
11 follow
Forum: Have we failed as bioinformatician in this time of COVID-19
Bioinformatician COVID-19
updated 10 months ago by 43k • written 3.9 years ago by ▴ 10
34
votes
5
replies
2.3k
views
Forum: what we should reply when we are asked do you have any question?
career interview
updated 11 months ago by 43k • written 6.9 years ago by ★ 4.3k
33
votes
26
replies
3.6k
views
7 follow
Forum: Poll: How many people have PyPy?
pypy python
updated 12 months ago by 43k • written 8.1 years ago by 13k
33
votes
23
replies
4.1k
views
8 follow
Forum: Is there a file format better suited for the era of pangenomics than the .vcf? What are its attributes?
VCF file-format genomics
updated 10 months ago by 22k • written 10 months ago by 4.2k
1,000 results • Page 3 of 20
Recent Votes
High fold change values
C: Bcftools Mpileup Removes Marked Duplicates?
Prediction tools summary - zero values
A: Error with Formatdb of legacy blast?
Error with Formatdb of legacy blast?
Answer: How to convert SAM/BAM file to GTF/GFF file?
A: Ribo-seq vs RNA-seq read count
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Comment: ISCN annotation for SV/CN VCF files by adedare • 0
https://res.saletool.cn/vcf/18084521725806
Comment: ISCN annotation for SV/CN VCF files by adedare • 0
https://res.saletool.cn/vcf/18084435127462
Comment: How can I solve this error? by 22211020193 • 0
I want to conduct GWAS meta-analysis using metal software.
Comment: How to reduce the variation of CIBERSORTx results? by feather-W • 0
Hi LChart, Thanks for your reply. Yes, the identical command lines produce different results in CIBERSORTx S-mode. Although I input the da…
Comment: Removing duplicates by joe ▴ 490
I *could* imagine an ONT scenario where you'd want to remove duplicates (amplicon-seq, etc) but likely for variant calling there is no need…
Comment: Removing duplicates by samuel.a.odonnell ▴ 510
As above, you do not need to worry about duplicates for ONT data Also have you looked at using other tools for variant calling more suited…
Answer: Pediococcus acidilactici sequence analysis by geneontologyhelp ▴ 340
If you're looking for the GO annotations for *Pediococcus acidilactici*, these are available. Using the instructions under [*2. All other…
Answer: What is the normalization status of metabric data? by etiennedanis ▴ 10
I just found an answer to this question here: [https://groups.google.com/g/cbioportal/c/hm_i4jbG1uc/m/h9rNYOvpAAAJ][1] [1]: https://gro…
Comment: Removing duplicates by joe ▴ 490
With ONT you will only have PCR duplicates. 'Read' (you mean optical?) duplicates are an artifact of cluster-based sequencing (ie Illumina)…
Comment: assembly using CCS, CLR, CCS_CLR sequences together? by GenoMax 141k
PacBio has some recommendations on software for genome assemblies on this page (which you may have seen but just in case): https://www.pacb…
Answer: DEG analysis of RNA-seq data across multiple tissues and two conditions by swbarnes2 14k
I would not put totally different tissues in the same DESeq object. I don't think that's going to do good things for normalization or disp…
Comment: Removing duplicates by quentinperriere • 0
pcr duplicates or/and read duplicates should I use this command to remove them ? or we don't talk about duplicates when dealing with ONT ??…
Comment: How to reduce the variation of CIBERSORTx results? by LChart 3.9k
If you didn't change the input signature matrix, the input RNA matrix, or the input parameters - what did you change? Is the statement that…
Answer: Inconsistency in SNP detection pipelines for multi-sample analysis by LChart 3.9k
> To replicate GVCF behavior and avoid issues with 0/0 genotypes appearing as missing, I don't use the -v option I'm not sure what you m…
Comment: Supergnova stuck in tutorial by GenoMax 141k
Your best option may be to email the author (and/or the senior author from the paper). Let them know that the FTP links mentioned on GitHub…
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