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Thank you very much!
Comment: I'm new to cell ranger. Can single cells prepared with a protocol other than the
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Thank you so much! If the 10x Chromium protocol is not used, should the processing be done with HISAT2, FeatureCounts, and so on?
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For the paper, they used the `indrop` pipeline (https://github.com/indrops/indrops), and you should have a look at it, and try to run with …
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CellRanger is specific for 10x Chromium. I did not check the details of this single-cell platform so I recommend to see what papers that us…
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See https://www.biostars.org/p/9486493 and parameter `-max_hsps 1`
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Since the module detection is performed on the TOM, you should go for the TOM.
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Thank you! Also, cell typist would successfully identify the above cell types, however, they seemed to be in a pretrained or pre-organized …
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