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it worked. Thank you. Now my terminal is crashing, seems 16gb of RAM and 4 cores isn't enough. Can you suggest something?
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I checked Picard ```AddOrReplaceReadsGroups``` and ```AddOATag```. Is that what you're referring to?
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Noted that 'accurate' wasn't among your criteria ;)
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> server so I cannot use Blat for this purpose.
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My favourite for this task would be [GMAP]( http://research-pub.gene.com/gmap ), which can produce GFF format with option `-f 2`.
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I'd try those OP @samuel recommends
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No. Find a tutorial, try it, then if you have specific questions, ask of the galaxy help site.
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