Limit : all time
all time today this week this month this year
1,000 results • Page 3 of 20
Sort: replies
Rank Views Votes Replies
8
votes
42
replies
3.5k
views
NaS (Nanopore Synthetic-long) help
Assembly preprocessing
updated 4 weeks ago by 37k • written 7.7 years ago by ▴ 290
166
votes
41
replies
83k
views
27 follow
Multiline Fasta To Single Line Fasta
sequence fasta
updated 6 days ago by • 0 • written 11.6 years ago by ▴ 240
19
votes
41
replies
6.9k
views
7 follow
n.sv number for batch effects in RNA-seq
RNA-Seq
updated 5.5 years ago by ★ 5.2k • written 5.5 years ago by ▴ 70
5
votes
41
replies
1.7k
views
Insert sequence in nt database
nt
2.7 years ago by anasofiamoreira94 ▴ 80
38
votes
41
replies
47k
views
9 follow
Volcano Plot from DEseq2
R
written 5.2 years ago by ★ 1.1k
16
votes
41
replies
7.6k
views
How to extract information from headers of fasta file
sequence
updated 7 months ago by 37k • written 7.9 years ago by ▴ 70
13
votes
41
replies
1.5k
views
Is my file created completely
sam file BWA MEM
updated 5.7 years ago by 34k • written 5.8 years ago by ▴ 10
136
votes
41
replies
20k
views
20 follow
Forum: Are We Rude/Do We Expect Too Much From People Asking Questions On This Forum?
Forum
updated 9.3 years ago by ★ 1.1k • written 9.7 years ago by ★ 1.6k
23
votes
41
replies
12k
views
10 follow
WGCNA modules and categorical traits relationship
WGCNA
updated 11 weeks ago by • 0 • written 5.1 years ago by ▴ 110
98
votes
41
replies
4.3k
views
17 follow
Forum: I am really pissed off by the bioinformatics software world. Do/can we have a better solution?
software error sequencing next-gen Forum
updated 6.4 years ago by 96k • written 6.4 years ago by ▴ 500
18
votes
41
replies
10k
views
6 follow
paired end illumina reads
Assembly paired end
updated 6 months ago by 37k • written 7.3 years ago by ▴ 290
59
votes
41
replies
18k
views
16 follow
Tutorial: Polish PacBio assembly with latest PacBio tools : an affordable solution for everyone
arrow pbalign pacbio polishing smrt limk Tutorial
updated 2.8 years ago by • 0 • written 5.4 years ago by ★ 1.4k
78
votes
41
replies
27k
views
24 follow
Tutorial: Extract Total Non-Overlapping Exon Length Per Gene With Bioconductor
fpkm rna-seq bioconductor
updated 10 weeks ago by • 0 • written 9.3 years ago by ★ 7.6k
202
votes
41
replies
23k
views
20 follow
Forum: Genomics is not Special. Computational Biologists are reinventing the wheel for big data biology analysis
genomics cram gatk galaxy
updated 10 months ago by 37k • written 8.2 years ago by ★ 5.1k
31
votes
41
replies
1.3k
views
12 follow
Forum: Question regarding journal publications
journals publications Forum
2.9 years ago by K.Gee ▴ 40
17
votes
40
replies
15k
views
16 follow
Tutorial: Easy way to run easily orthoMCL (Copy & paste)
run easy orthomcl all-v-all mcl Tutorial
updated 2.3 years ago by ★ 2.0k • written 6.6 years ago by ▴ 160
40
votes
40
replies
9.1k
views
9 follow
What is the reason for trimming reads to 30 bp for ATAC-seq aligning?
ATAC-seq alignment
updated 5.0 years ago by 84k • written 6.3 years ago by ▴ 520
67
votes
40
replies
8.0k
views
12 follow
Forum: I want to re-open the old debate: python or perl ?
perl python
updated 11 months ago by 37k • written 8.2 years ago by ★ 2.9k
6
votes
40
replies
8.9k
views
Fastx_Collapser Has No Fastq Output
bioinformatics fastx rna-seq
updated 6.3 years ago by ▴ 50 • written 9.4 years ago by ▴ 240
68
votes
40
replies
4.7k
views
11 follow
Can We Agree On A Short Twitter Hashtag For Nextgen Sequencing?
next-gen sequencing sequencing
updated 11.3 years ago by ▴ 310 • written 11.3 years ago by ★ 1.3k
16
votes
40
replies
4.6k
views
About TCGA CNV data preprocessing
SNP CNV
updated 4.3 years ago by 84k • written 4.3 years ago by ▴ 40
10
votes
40
replies
4.8k
views
weird insert size post trimming
RNA-Seq
updated 5.5 years ago by ★ 2.9k • written 5.5 years ago by ▴ 280
151
votes
39
replies
22k
views
20 follow
Forum: A Farewell To Bioinformatics
bioinformatics Forum
updated 5.3 years ago by 84k • written 10.0 years ago by 3.0k
20
votes
39
replies
7.6k
views
7 follow
Is Blast+ Running As Fast As It Could ?
blast blast memory
updated 7.4 years ago by ▴ 150 • written 11.1 years ago by ▴ 160
55
votes
39
replies
4.4k
views
8 follow
Programming Challenge - Synthetic Whole Genome Vcf
vcf python perl awk
updated 10.4 years ago by 8.2k • written 10.4 years ago by ▴ 310
273
votes
39
replies
162k
views
29 follow
Tutorial: How to download raw sequence data from GEO/SRA
fastq SRA download GEO bam
updated 9 months ago by 37k • written 8.4 years ago by 20k
25
votes
39
replies
4.4k
views
8 follow
Forum: Bioinformatics Study Group
bioinformatics Forum
9.5 years ago by Olivier ▴ 440
18
votes
39
replies
2.7k
views
Tool: CBioInfCpp.h as a C++ lib containing some functions for bioinformatics
Tool C++ tool c++
23 months ago by chernouhov sergey ▴ 50
36
votes
39
replies
2.6k
views
11 follow
Forum: Survey/Vote: If you could double the speed of any three commandline tools, which three would they be?
RNA-Seq alignment blast next-gen sequencing Forum
updated 5.2 years ago by ▴ 190 • written 5.2 years ago by ▴ 130
226
votes
39
replies
280k
views
33 follow
Gene Id Conversion Tool
mapping conversion
updated 5 months ago by 37k • written 13.3 years ago by ▴ 620
66
votes
39
replies
6.2k
views
13 follow
What Do You Consider The Most Trivial And The Most Challenging Tasks In Your Particular Field Of Work?
career subjective
updated 22 months ago by 37k • written 12.9 years ago by ▴ 310
11
votes
39
replies
2.8k
views
Any advice for a de novo genome assembly
k-mer de novo genome assembly
updated 6.2 years ago by ▴ 60 • written 6.2 years ago by ▴ 630
69
votes
39
replies
3.2k
views
14 follow
Forum: Preparation for Bioinformatics.SE closing
biostars Forum
updated 5.7 years ago by ▴ 750 • written 5.7 years ago by 13k
81
votes
39
replies
5.9k
views
11 follow
News: State Of Biostar - Future Directions (January 2013)
biostar News
updated 10.0 years ago by ★ 2.5k • written 10.1 years ago by 96k
39
votes
38
replies
6.9k
views
9 follow
DNA composition - all k-mers and their frequency in some sequencing data
sequencing
updated 5 months ago by 37k • written 7.2 years ago by 13k
16
votes
38
replies
15k
views
Differential gene Analysis by Limma
Limma Microarray R
updated 9 months ago by 37k • written 8.0 years ago by ▴ 920
53
votes
38
replies
3.2k
views
10 follow
Multiple alignment software
alignment clustal omega tcoffee
updated 3.4 years ago by 96k • written 3.4 years ago by ▴ 130
159
votes
38
replies
15k
views
22 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
bioinformatics Forum humor
updated 4 months ago by ▴ 30 • written 9.9 years ago by 96k
220
votes
38
replies
132k
views
29 follow
Tool: Converting Genome Coordinates From One Genome Version To Another (Ucsc Liftover, Ncbi Remap, Ensembl Api)
genome coordinates conversion liftover ensembl Tool
updated 7 weeks ago by ▴ 90 • written 9.9 years ago by 19k
26
votes
38
replies
16k
views
Heatmap based with FPKM values
RNA-Seq next-gen R
updated 3.7 years ago by 20 • written 5.2 years ago by ▴ 780
48
votes
38
replies
15k
views
8 follow
Forum: Eukaryotic Genome Annotation in 2016
genome annotation software Forum
updated 6.9 years ago by ▴ 360 • written 6.9 years ago by 11k
89
votes
38
replies
124k
views
18 follow
Extract Reads From A Bam File That Fall Within A Given Region
bam
updated 5 months ago by ▴ 10 • written 10.6 years ago by ▴ 370
129
votes
38
replies
5.4k
views
21 follow
Forum: Who are the Greatest Bioinformaticians Of All Time (GBOAT)
bioinformatics Forum
updated 3.8 years ago by 34k • written 3.8 years ago by 96k
47
votes
37
replies
7.1k
views
12 follow
Forum: The Organisation Of Really Contrived Acronyms (Orca)
Forum
updated 9.5 years ago by 21k • written 9.5 years ago by 2.3k
29
votes
37
replies
2.3k
views
8 follow
Perl/Python script: phased vcf to phased tped
vcf ped phased
4.3 years ago by Shicheng Guo ★ 9.2k
43
votes
37
replies
1.5k
views
7 follow
Forum: Checking the quality and reliability in bioinformatics
bioinformatics Forum
updated 5.3 years ago by 47k • written 5.3 years ago by ★ 4.1k
130
votes
37
replies
12k
views
27 follow
What Are The Obsolete Research Topics In Bioinformatics?
updated 11.4 years ago by ★ 1.3k • written 11.4 years ago by ▴ 820
17
votes
37
replies
3.2k
views
No alias or index file found for protein database
next-gen software error sequence
updated 3.7 years ago by 20 • written 3.8 years ago by ▴ 470
110
votes
37
replies
6.8k
views
13 follow
Bioinformatics Career Survey 2011/2012
career job
updated 11.0 years ago by 2.3k • written 11.4 years ago by 152k
18
votes
37
replies
11k
views
11 follow
Is it possible to download the full EpiCov database from GISAID?
GISAID genome Database EpiCoV
updated 21 months ago by ▴ 240 • written 2.9 years ago by ▴ 30
1,000 results • Page 3 of 20
Recent Votes
Comment: fastqc: Per base sequence content
Comment: fastqc: Per base sequence content
Comment: Size of two scRNA-Seq conditions
Answer: Dealing with missing (NA) values in my 450K methylation array dataset
What type of database does gnomAD use?
What type of database does gnomAD use?
Answer: What type of database does gnomAD use?
Recent Locations • All
Bergen, Norway, just now
France/Nantes/Institut du Thorax - INSERM UMR1087, just now
Germany, 1 minute ago
United States, 2 minutes ago
Portugal, 2 minutes ago
Italy, 4 minutes ago
Germany, 4 minutes ago
Recent Awards • All
Scholar to joe ▴ 380
Popular Question to Matteo Schiavinato ★ 3.6k
Popular Question to Barry Digby ★ 1.1k
Popular Question to Lee Katz ★ 3.1k
Scholar to GenoMax 125k
Scholar to Nicolas Rosewick 10k
Popular Question to Papyrus ★ 2.3k
Recent Replies
Answer: Mapping with bbmap.sh by Mensur Dlakic ★ 22k
Definitely not normal. It doesn't seem that BBmap is taking any reads in. That's why your `num reads` and `num bases` are all zeros, which…
Comment: how to get an average ROC curve after 10-fold cross validation in r by Mensur Dlakic ★ 22k
I have no idea what cutoff is in this context, nor what you mean by `original` values. I don't know how you got those values for means. I …
Comment: Protein protein interactions by Mensur Dlakic ★ 22k
This is not a trivial calculation nor a simple sequence comparison like with BLAST, so it may take a while. Besides, there could be hundred…
Comment: snakemake Unexpected keyword bam in rule definition by Wayne ★ 1.6k
Any chance it is an indentation issue that isn't caused by what you aren't sharing here? See [here](https://groups.google.com/g/snakemake/c…
Comment: Size of two scRNA-Seq conditions by rpolicastro 11k
More important would be the cell QC stats (such as reads per cell and number of unique features detected per cell), the number of cells per…
Answer: What type of database does gnomAD use? by Jeremy Leipzig 21k
If you primarily want a variant warehouse that supports genomic region and sample queries at biobank scale, you should look into [TileDB-VC…
Answer: Dealing with missing (NA) values in my 450K methylation array dataset by Basti ★ 1.3k
You could omit the NA values : `pca <- prcomp(na.omit(t(exprs(GSE65820))))`
Comment: Virtual box by Ram 37k
This post does not fit the theme of this forum.
Comment: Filter InterProScan output for a specific function by jv ▴ 790
I imagine you can filter on things like E.C. numbers and domain names using `bash`, `awk`, `R`, or some other code.
Comment: How to find the most frequent alternative-splicing event from DEXSEQ data? by STARDUST • 0
I will try both rMATS and SUPPA for categorising changes. For the specific exons, i'm expecting the last exons should be affected. That is …
Answer: snp Caenorhabditis elegans by GenoMax 125k
See: https://www.elegansvariation.org/data/release/20220216
Answer: want the codes for cuffdiff , NIOSeq , bayseq to perform DEGs. by jv ▴ 790
- NOISeq <https://www.bioconductor.org/packages/release/bioc/vignettes/NOISeq/inst/doc/NOISeq.pdf> - Cuffdiff <http://cole-trapnell-lab.gi…
Comment: fastqc: Per base sequence content by jv ▴ 790
I think this is to be expected based a report I was able to find online <https://uu.diva-portal.org/smash/get/diva2:1608120/FULLTEXT01.pd…
Comment: fastqc: Per base sequence content by GenoMax 125k
Unless the kit directions tell you to remove remove the initial 10-15 bp you may as well leave them alone. Data should align would be my in…
Comment: RNA Editing data from RNA-seq by Genetics • 0
Thank you so much for your kind reply and for helping a bioinformatics beginner like me. Can I use **bowtie2** tools for allignment and th…
Traffic: 3051 users visited in the last hour
Content Search
Users
Tags
Badges
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6