Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : all time
all time
today
this week
this month
this year
1,000 results • Page
5 of 20
Sort: Votes
Rank
Views
Votes
Replies
81
votes
26
replies
13k
views
17 follow
Appropriate Podcasts For A Bioinformatician?
subjective
updated 6 months ago by
Ram
43k • written 14.1 years ago by
User 59
13k
81
votes
21
replies
42k
views
15 follow
How To Get Bed File Containing Exons Of Canonical Transcripts And Their Corresponding Gene Symbols
bed
transcript
ucsc
biomart
updated 7 months ago by
Joel Wallenius
▴ 210 • written 10.2 years ago by
pristanna
▴ 750
81
votes
4
replies
21k
views
Discrimination Between Germline And Somatic Mutations In Tumor Without The Availability Of The Normal Paired Sample
mutation
somatic
updated 11.2 years ago by
Stefano Berri
4.4k • written 11.2 years ago by
Fred Fleche
4.3k
81
votes
39
replies
7.5k
views
11 follow
News:
State Of Biostar - Future Directions (January 2013)
biostars
updated 14 months ago by
Ram
43k • written 11.3 years ago by
Istvan Albert
100k
81
votes
27
replies
20k
views
12 follow
ATAC-seq sample normalization
ChIP-Seq
atac-seq
normalization
updated 20 days ago by
sgv
• 0 • written 4.3 years ago by
Flo
▴ 240
81
votes
6
replies
45k
views
6 follow
Read Group In Sam/Bam Files: What Do They Exactly Describe?
bam
samtools
next-gen
updated 2.1 years ago by
Ram
43k • written 12.0 years ago by
Stefano Berri
4.4k
81
votes
15
replies
24k
views
10 follow
Vertebrate Subset Nr Database? Build My Own?
blast
database
updated 9.9 years ago by
jcsoellner
• 0 • written 13.1 years ago by
Jason
▴ 430
81
votes
23
replies
12k
views
10 follow
Transform A Pipeline Of R Scripts Into A Web Application
webservice
web
pipeline
galaxy
updated 24 months ago by
Ram
43k • written 13.7 years ago by
Giovanni M Dall'Olio
28k
80
votes
21
replies
36k
views
17 follow
How to know that your RNA-seq is stranded or not?
RNA-Seq
updated 5 months ago by
kathryn.jacksonjones
• 0 • written 10.0 years ago by
M K
▴ 660
80
votes
27
replies
56k
views
20 follow
How To Write Data In A Granges Object To A Bed File.
bed
updated 20 months ago by
Johan Zicola
▴ 70 • written 10.4 years ago by
Ram
▴ 190
80
votes
23
replies
5.3k
views
11 follow
Forum:
Are we facing a bioinformatician shortage?
salary
career
updated 13 months ago by
Ram
43k • written 9.5 years ago by
Christian
★ 3.0k
80
votes
24
replies
11k
views
18 follow
Which Bioinformatics Journals Do You Follow?
bioinformatics-journal
updated 5 months ago by
Ram
43k • written 14.0 years ago by
Giovanni M Dall'Olio
28k
80
votes
34
replies
5.2k
views
11 follow
Forum:
Authorship issues- how to resolve?
authorship
updated 15 months ago by
Ram
43k • written 8.9 years ago by
datanerd
▴ 520
80
votes
22
replies
91k
views
16 follow
How To Split One Big Sequence File Into Multiple Files With Less Than 1000 Sequences In A Single File
next-gen
sequencing
fasta
rna
r
perl
updated 17 months ago by
Ram
43k • written 12.5 years ago by
Hamilton
▴ 290
79
votes
16
replies
48k
views
11 follow
How do you cite a tool that doesn't have a publication?
citation
academic
style
updated 8.0 years ago by
Emily
23k • written 8.1 years ago by
novice
★ 1.1k
79
votes
35
replies
9.6k
views
17 follow
Forum:
Do bioinformaticians often break molecular biologists' hearts by being the first author?
authorship
career
updated 11 months ago by
jli.ww
• 0 • written 8.4 years ago by
sentausa
▴ 650
79
votes
86
replies
31k
views
20 follow
Tool:
Introducing Clumpify: Create 30% Smaller, Faster Gzipped Fastq Files. And remove duplicates.
clumpify
storage
bbmap
pigz
compression
updated 10 months ago by
Ram
43k • written 7.4 years ago by
Brian Bushnell
20k
78
votes
38
replies
33k
views
15 follow
Illumina Instrument Type from fastq?
fastq
updated 7 weeks ago by
nickp60
▴ 60 • written 7.8 years ago by
andrew.j.skelton73
6.5k
78
votes
31
replies
6.8k
views
14 follow
Forum:
Do Bad Questions Even Exist?
biostars
general
education
updated 14 months ago by
Ram
43k • written 13.2 years ago by
Chris Evelo
10k
77
votes
9
replies
13k
views
15 follow
Which program, tool, or strategy do you use to visualize genomic rearrangements?
fusion-genes
visualizations
rearrangements
updated 19 months ago by
Ram
43k • written 8.6 years ago by
stianlagstad
★ 1.1k
77
votes
13
replies
15k
views
12 follow
Tutorial:
Exploring cancer mutation data portals
TCGA
Cancer
ICGC
Data-Portal
Mutation
updated 2.5 years ago by
Ram
43k • written 9.9 years ago by
Malachi Griffith
19k
77
votes
24
replies
61k
views
16 follow
How To Sort Bed Format File
bed
updated 18 months ago by
Pierre Lindenbaum
161k • written 11.2 years ago by
lyz10302012
▴ 450
77
votes
22
replies
23k
views
14 follow
Assessing The Quality Of De Novo Assembled Data
assembly
next-gen
updated 19 months ago by
Ram
43k • written 11.9 years ago by
Prakki Rama
★ 2.7k
77
votes
18
replies
46k
views
13 follow
Bioinformaticist Vs. Bioinformatician
subjective
updated 21 months ago by
Dan
• 0 • written 13.9 years ago by
Science_Robot
★ 1.1k
77
votes
20
replies
14k
views
12 follow
Perl Or Python For Comparative Genomics?
bioperl
biopython
python
comparative
updated 5.9 years ago by
lakhujanivijay
5.8k • written 13.6 years ago by
Dave Lunt
★ 2.0k
77
votes
15
replies
10k
views
10 follow
Best Bioinformatics Research Images
visualization
updated 12.8 years ago by
Simon Cockell
7.4k • written 12.9 years ago by
Niallhaslam
2.3k
77
votes
11
replies
33k
views
9 follow
Star Or Tophat?
rnaseq
alignment
tophat
updated 3.0 years ago by
Ram
43k • written 10.3 years ago by
lkmklsmn
▴ 970
77
votes
10
replies
21k
views
6 follow
what do the headers of the rMATS output files mean?
MATS
Alternative splicing
next-gen
STAR
updated 5.5 years ago by
drskm7
• 0 • written 6.9 years ago by
atcggcta
▴ 140
77
votes
16
replies
34k
views
14 follow
Automatically Getting The Ncbi Taxonomy Id From The Genbank Identifier
genbank
conversion
ncbi
taxonomy
updated 7.2 years ago by
-_-
★ 1.1k • written 12.7 years ago by
Alf
▴ 490
77
votes
12
replies
39k
views
8 follow
How Can I Get The Human Chromosome Centromere Position And Chromosome Length In Grch37/Hg19
position
ucsc
updated 5.5 years ago by
Shixiang
▴ 100 • written 13.6 years ago by
Jdk
▴ 260
76
votes
71
replies
19k
views
19 follow
Tutorial:
Clustering of DAVID gene enrichment results from gene expression studies
enrichment
david
updated 1 day ago by
GenoMax
141k • written 6.2 years ago by
Kevin Blighe
87k
76
votes
14
replies
12k
views
10 follow
Tool:
karyoploteR: uncircle your genomes
karyoploteR
Rdataviz
NGS
updated 10 months ago by
Ram
43k • written 6.9 years ago by
bernatgel
★ 3.4k
76
votes
26
replies
120k
views
15 follow
How to count fastq reads
sequence
next-gen
fastq
reads
updated 5 months ago by
cschu181
★ 2.8k • written 9.0 years ago by
Chenglin
▴ 260
76
votes
24
replies
20k
views
18 follow
What Methods Do You Use For Short Read Mapping?
short-read-aligner
sequence
updated 4.7 years ago by
Ram
43k • written 14.1 years ago by
Biostar User
★ 1.0k
76
votes
14
replies
16k
views
15 follow
Tutorial:
List Of Bioinformatics Books
books
updated 14 months ago by
Ram
43k • written 10.9 years ago by
Medhat
9.7k
76
votes
22
replies
54k
views
17 follow
How To Download All Sra Samples At Once ?
geo
sra
updated 13 months ago by
Ram
43k • written 10.2 years ago by
biorepine
★ 1.5k
75
votes
19
replies
3.4k
views
11 follow
Blog:
A list of technical advantages of Snakemake over Nextflow | Cloud solution for Snakemake
Snakemake
updated 7 weeks ago by
LauferVA
4.2k • written 7 months ago by
Kenny
▴ 30
75
votes
15
replies
36k
views
8 follow
About Paired-End Sequencing
next-gen-sequencing
duplicates
updated 5 months ago by
Ram
43k • written 14.0 years ago by
Pierre Lindenbaum
161k
75
votes
13
replies
67k
views
10 follow
Tutorial:
Machine Learning For Prediction of Relapse in Cancer - Part 2 - Building A Random Forest Classifier
r
cancer
classification
updated 17 months ago by
Ram
43k • written 10.4 years ago by
Nicholas Spies
★ 1.2k
74
votes
14
replies
30k
views
12 follow
Forum:
Bioinformatics Consulting Thoughts
consulting
updated 22 months ago by
Ram
43k • written 13.9 years ago by
Will
4.5k
74
votes
16
replies
4.7k
views
10 follow
Tools & Techniques Bioinformatician Have To Master?
subjective
updated 10 months ago by
Ram
43k • written 13.1 years ago by
Thaman
★ 3.3k
74
votes
3
replies
39k
views
Tutorial:
Revisiting the FastQC read duplication report
fastqc
updated 2.4 years ago by
Ram
43k • written 9.8 years ago by
Istvan Albert
100k
73
votes
14
replies
4.2k
views
12 follow
Forum:
Things you wish you knew when entering the bioinformatics field
pitfalls
advice
updated 10 months ago by
Ram
43k • written 3.6 years ago by
jared.andrews07
★ 16k
73
votes
7
replies
13k
views
6 follow
What Should I Use Blat For?
alignment
updated 11.4 years ago by
David Quigley
11k • written 11.4 years ago by
KCC
★ 4.1k
73
votes
60
replies
27k
views
16 follow
Tutorial:
Produce PCA bi-plot for 1000 Genomes Phase III in VCF format (old)
1000genomes
PLINK
PCA
VCF
updated 14 months ago by
Ram
43k • written 6.6 years ago by
Kevin Blighe
87k
73
votes
27
replies
28k
views
16 follow
Tutorial:
How To Separate Illumina Based Strand Specific Rna-Seq Alignments By Strand
RNA-seq
updated 4 months ago by
snardeli
• 0 • written 10.2 years ago by
Istvan Albert
100k
73
votes
25
replies
9.0k
views
16 follow
The Five Most Annoying Bioinformatics Problems You Face Every Week?
updated 10.6 years ago by
Mary
11k • written 10.6 years ago by
davidcassali
▴ 60
72
votes
18
replies
7.5k
views
17 follow
Forum:
What Is One Book Every Bioinformatician Should Read?
books
updated 14 months ago by
Ram
43k • written 10.8 years ago by
Philipp Bayer
8.3k
72
votes
20
replies
26k
views
11 follow
Parsing Ncbi Taxonomic Tree?
ncbi
taxonomy
python
tree
updated 16 months ago by
Ram
43k • written 12.5 years ago by
Prohan
▴ 350
72
votes
48
replies
18k
views
15 follow
Tutorial:
For Short Reads, Which Aligners Find All Hits Given Certain Edit Distance Threshold?
ngs
alignment
bwa
updated 14 months ago by
Ram
43k • written 11.2 years ago by
lh3
33k
1,000 results • Page
5 of 20
Recent Votes
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
RNA-seq power estimation using ssizeRNA program
Answer: WGCNA preservation analysis
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
ISCN annotation for SV/CN VCF files
Comment: How to convert plink files to Hapmap Format
Am I crazy, or are most published RNA-seq studies vastly underpowered?
Recent Locations •
All
United States,
just now
United States,
2 minutes ago
France/Nantes/Institut du Thorax - INSERM UMR1087,
3 minutes ago
United States,
5 minutes ago
United States,
5 minutes ago
United States,
5 minutes ago
United Kingdom,
5 minutes ago
Recent Awards •
All
Popular Question
to
AdviceSeeker
• 0
Great Question
to
Ann
★ 2.4k
Scholar
to
marco.barr
▴ 80
Popular Question
to
Kash
▴ 110
Scholar
to
GenoMax
141k
Scholar
to
anovak
▴ 120
Popular Question
to
pfee418
▴ 10
Recent Replies
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
GenoMax
141k
You simply need to make a list of barcodes one on each line: https://kb.10xgenomics.com/hc/en-us/articles/115004506263-What-is-a-barcode-wh…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
GenoMax
141k
> What specifications and operating system would be ideal for my case? For choice of OS: Linux to access the large pool of open source sof…
Comment: How to convert plink files to Hapmap Format
by
bk11
★ 2.3k
Ok. First update your phenotypes because in the 6th column all the values are -9 meaning missing, and then run `--assoc`. You can do the fo…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
i.sudbery
19k
You'll probably get away with using one of the other analysis pipelines, like Alevin-fry with much less than 128GB of RAM. Workstations can…
Comment: Difference between samtools "-f 9" and "-f 11"
by
Dylan
• 0
> (which is weird, if it's in proper pair, read and its mate should be both mapped) So that was my thought as well, I think the code you l…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
Ram
43k
> several single cell samples At the same time? You need a server, not a PC. Google around (and search the site as well with keyword "conf…
Answer: Difference between samtools "-f 9" and "-f 11"
by
Pierre Lindenbaum
161k
9: read must be paired AND mate must be unmapped 11: read must be paired AND mapped in proper pair AND mate must be unmapped (which is w…
Comment: How to convert plink files to Hapmap Format
by
Sofia
• 0
the data is binary and associated with a disease but we're studying only the cases and there are other phenotypes of severity
Comment: Cell barcode whitelists for DNBelab C Series High-throughput Single-cell RNA Ser
by
atowns21
• 0
could you share an example command of using STARSolo for the alignment? Did you use the json file directly? Or do you have to re-format the…
Comment: How to trim transcripts using information from NCBI contamination screen report
by
GenoMax
141k
> I didn't do a good job with Trimmomatic for some reason so I have to do some trimming of my transcripts. If you had extraneous sequence…
Comment: How to convert plink files to Hapmap Format
by
bk11
★ 2.3k
Please answer my above question. What type of data is it, binary (cases and controls) or quantitative? You need to update Phenotype info in…
Comment: How to convert plink files to Hapmap Format
by
Sofia
• 0
This is how my .fam file looks like ![enter image description here][1] [1]: /media/images/caccd97c-3606-48d2-aa32-423a9882 And I run …
Comment: How to convert plink files to Hapmap Format
by
bk11
★ 2.3k
What type of phenotype is in your data? It looks like it does not have affection status (default values for affection status will be: 1 = u…
Comment: Redirection of Duplicate PMIDs
by
LauferVA
4.2k
Agree. This is also a great solution that flips the problem on its head, as it were, Dominick. I dont want to be preachy, but this is why i…
Answer: Redirection of Duplicate PMIDs
by
GenoMax
141k
> linked to a list of grants Using [**EntrezDirect**][1] this may be much simpler if you have the grant number available. I tested this w…
Traffic: 2610 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6