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Limit : this month
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1,000 results • Page
1 of 20
Sort: Votes
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Views
Votes
Replies
362
votes
82
replies
143k
views
35 follow
Tool:
Gnu Parallel - Parallelize Serial Command Line Programs Without Changing Them
parallel
next-gen
ngs
updated 24 days ago by
jena
▴ 290 • written 11.2 years ago by
ole.tange
★ 4.4k
164
votes
70
replies
60k
views
34 follow
How To Efficiently Parse A Huge Fastq File?
fastq
next-gen-sequencing
updated 22 days ago by
Ram
43k • written 12.8 years ago by
Panos
★ 1.8k
105
votes
15
replies
112k
views
16 follow
Convertion Of Gff3 To Gtf
gtf
gff
updated 29 days ago by
xiaoleiusc
▴ 140 • written 11.9 years ago by
GouthamAtla
12k
103
votes
42
replies
31k
views
34 follow
Forum:
List of cloud genomics companies
cloud-genomics
updated 10 days ago by
Jeremy Leipzig
22k • written 10.4 years ago by
14134125465346445
★ 3.6k
98
votes
106
replies
46k
views
26 follow
Tutorial:
Produce PCA bi-plot for 1000 Genomes Phase III - Version 2
1000genomes
PLINK
PCA
updated 11 days ago by
psb7
▴ 20 • written 5.6 years ago by
Kevin Blighe
87k
87
votes
24
replies
23k
views
17 follow
Tutorial:
Gene Set Enrichment Analysis
microarray
enrichment
RNA-Seq
GSEA
updated 1 day ago by
Gordon
• 0 • written 3.5 years ago by
Hamid Ghaedi
3.2k
81
votes
27
replies
20k
views
12 follow
ATAC-seq sample normalization
ChIP-Seq
atac-seq
normalization
updated 16 days ago by
sgv
• 0 • written 4.3 years ago by
Flo
▴ 240
57
votes
22
replies
28k
views
7 follow
Batch correction in DESeq2
Combat
DESeq2
Batch-Effect
updated 13 days ago by
Ram
43k • written 4.5 years ago by
Arindam Ghosh
▴ 510
54
votes
12
replies
28k
views
12 follow
Out Of Disk Space With Picard Tools ?
picard
updated 1 day ago by
kjngo
• 0 • written 12.0 years ago by
Gabriel R.
★ 2.9k
49
votes
30
replies
11k
views
13 follow
Tutorial:
How to Use Biostars, Part-I: Questions, Answers, Comments and Replies
documentation
meta
how-to
updated 22 days ago by
Istvan Albert
100k • written 8.1 years ago by
Ram
43k
39
votes
9
replies
42k
views
6 follow
Batch effects : ComBat or removebatcheffects (limma package) ?
limma
sva
Combat
batch-effect
updated 2 days ago by
Ram
43k • written 6.7 years ago by
lessismore
★ 1.3k
37
votes
5
replies
13k
views
How do you generate TMM normalized counts using EdgeR?
R
RNA-seq
edgeR
updated 9 days ago by
inedraylig
▴ 60 • written 2.9 years ago by
Pratik
★ 1.0k
34
votes
16
replies
20k
views
9 follow
Filtration Of Reads With Length Lower Than 30 From Bam
read
length
bam
updated 10 hours ago by
Hagen
▴ 10 • written 10.2 years ago by
filipzembol
▴ 180
31
votes
14
replies
12k
views
11 follow
Finding Rrna Genes In Metagenomic Data
rna
rRNA
metagenomics
updated 24 days ago by
Ram
43k • written 13.5 years ago by
toshnam
▴ 650
30
votes
12
replies
3.8k
views
Trying to understand the maths behind one Limma function
limma
RNA-Seq
batch-effect
updated 4 days ago by
Ram
43k • written 4.0 years ago by
Mozart
▴ 330
26
votes
5
replies
23k
views
BLAST options: max_target_seqs and num_alignments
blast
updated 29 days ago by
Lada
▴ 30 • written 9.4 years ago by
biolab
★ 1.4k
23
votes
23
replies
9.8k
views
6 follow
How to see if adjusting batch effect in RNA-seq is working or not
RNA-Seq
removeBatchEffect
batch-effect
edger
updated 2 days ago by
Ram
43k • written 5.3 years ago by
iraia.munoa
▴ 130
23
votes
11
replies
4.8k
views
7 follow
Code golf: detecting homopolymers of length N in the (human) genome
code golf
fasta
homopolymer
repeat
updated 3 days ago by
Ram
43k • written 4.9 years ago by
WouterDeCoster
47k
22
votes
18
replies
6.1k
views
about batch correction in scRNA-seq
scRNA
scRNA-seq
batch-effect
updated 23 hours ago by
Ram
43k • written 4.5 years ago by
Bogdan
★ 1.4k
22
votes
14
replies
28k
views
7 follow
removing batch effects using ComBat and SVA
SVA
ComBat
R
Batch-effect
updated 11 days ago by
Ram
43k • written 7.9 years ago by
LJ
▴ 280
21
votes
6
replies
16k
views
survfit(Surv()) P-value interpretation for 3 survival curves?
survfit
Surv
survival curves
R
updated 26 days ago by
marshelma
• 0 • written 4.5 years ago by
JJDollar
▴ 130
18
votes
20
replies
4.4k
views
6 follow
How to identify significant differentially expressed genes and gene regulatory networks from microarray data.
microarray
differential-gene-expression
updated 10 days ago by
Ram
43k • written 7.5 years ago by
morteza.mahmoudisaber
▴ 80
17
votes
8
replies
12k
views
9 follow
MAF vs VAF
sequencing
updated 16 days ago by
AshleeThomson
▴ 70 • written 6.1 years ago by
lauren.wahyudi
▴ 50
17
votes
16
replies
14k
views
10 follow
How to extract the longest isoform from multi fasta file
perl
extract
fasta
python
isoform
updated 16 days ago by
Madeleine
• 0 • written 9.7 years ago by
jon.brate
▴ 290
16
votes
10
replies
1.0k
views
6 follow
Forum:
F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 21 days ago by
Madelaine Gogol
5.3k • written 22 days ago by
Ann
★ 2.4k
15
votes
10
replies
7.6k
views
6 follow
Intersect multiple BED files
bed
intersect
updated 22 days ago by
Alex Reynolds
35k • written 8.3 years ago by
int11ap1
▴ 470
15
votes
6
replies
666
views
Need consultation about my rna-seq workflow
RNA-seq
Gene-expression
updated 29 days ago by
Ram
43k • written 4 weeks ago by
markusz
▴ 10
15
votes
6
replies
1.2k
views
6 follow
VCF to human friendly form?
vcf
visualization
updated 4 days ago by
Pierre Lindenbaum
161k • written 19 months ago by
wormball
▴ 10
13
votes
13
replies
18k
views
9 follow
How Do I Translate Multiple (More Than 25000) Dna Sequences With Different Frames To Protein Seuquence?
dna
protein
updated 11 days ago by
ummeswaiba
• 0 • written 10.3 years ago by
biostar
▴ 170
13
votes
22
replies
11k
views
SVA : Setting up the data from an ExpressionSet and applying sva function to extimate batches
sva
combat
batch-effect
updated 2 days ago by
Ram
43k • written 6.8 years ago by
lessismore
★ 1.3k
13
votes
10
replies
6.4k
views
Adjusting for confounding variables in RNA-SEQ Differential Expression Analysis
Differential-Gene-Expression
RNA-Seq
Statistics
updated 10 days ago by
Ram
43k • written 5.7 years ago by
ucakhnd
▴ 40
12
votes
7
replies
6.9k
views
Forum:
Differences between SMART-seq2, SMART-seq3, and 10x
cell
smartseq
UMIs
single
10x
updated 25 days ago by
dsull
★ 5.8k • written 2.6 years ago by
hamarillo
▴ 70
12
votes
2
replies
423
views
News:
8th Berlin Summer School in NGS Data Analysis (June 10-14, 2024) - already half booked
Workshop
RNA-Seq
DNA-Seq
Illumina
9 days ago by
David Langenberger
11k
12
votes
17
replies
6.0k
views
9 follow
ACMG assignment tools
wes
acmg
WES
WGS
updated 15 days ago by
Zuber
• 0 • written 3.9 years ago by
Eugene A
▴ 180
12
votes
14
replies
2.4k
views
8 follow
Forum:
Recovering bam files after unknown deletion in the storage
photorec
bam
sequencing
written 4.9 years ago by
Gabriel Wajnberg
▴ 70
12
votes
4
replies
2.2k
views
long read + salmon? (transcript abundance)
long-read
salmon
updated 14 days ago by
Ram
43k • written 13 months ago by
shinyjj
▴ 50
12
votes
13
replies
4.4k
views
RNA-Seq DGE using PCA components as covariate
differential-gene-expression
RNA-Seq
glm
DESeq2
updated 10 days ago by
Ram
43k • written 6.2 years ago by
firestar
★ 1.6k
11
votes
9
replies
11k
views
10 follow
Genome assembly statistical tools
Assembly
statistics
updated 6 days ago by
skdv2522
• 0 • written 4.7 years ago by
margab
▴ 10
11
votes
8
replies
5.1k
views
Multi-mapping High with featureCounts but not STAR
RNA-Seq
sequencing
alignment
updated 29 days ago by
GenoMax
141k • written 4.9 years ago by
garbuzov
▴ 70
11
votes
22
replies
4.1k
views
No significant DEG: A request to double check my commands for limma.
limma
differential-gene-expression
updated 10 days ago by
Ram
43k • written 5.0 years ago by
RNAseqer
▴ 260
11
votes
7
replies
991
views
Shrink logFC values after edgeR
logFC
edgeR
updated 26 days ago by
Gordon Smyth
★ 7.0k • written 11 weeks ago by
arvind.1
▴ 10
11
votes
6
replies
501
views
Pangenome of plant
pangenome
plant
11 days ago by
analyst
▴ 20
11
votes
12
replies
757
views
Downloaded pdb's on rcsb.org
rcsb
pdb
updated 19 hours ago by
Ram
43k • written 1 day ago by
iamsmor
• 0
11
votes
9
replies
6.9k
views
How to choose method to integrate different patient sample together in Seurat
Seurat
scRNA
batch-effect
harmony
updated 2 days ago by
Ram
43k • written 4.2 years ago by
623202215
▴ 80
11
votes
11
replies
1.1k
views
7 follow
How I know if it is a batch effect or are real differences between my controls and treatments?
RNA-seq
Transcriptomics
ComBat-seq
Batch-Effect
updated 10 days ago by
GenoMax
141k • written 13 days ago by
alejandro.mejiam1
▴ 10
10
votes
7
replies
7.1k
views
6 follow
Convert GVF to VCF
vcf
updated 3 days ago by
george-hall-ucl
• 0 • written 9.2 years ago by
win
▴ 970
10
votes
37
replies
12k
views
21 follow
Convert Gene IDs of Tomato Sol Database
tomato
gene
updated 9 days ago by
aziz.hammou
• 0 • written 8.8 years ago by
kevluv93
▴ 170
10
votes
36
replies
5.8k
views
Design in DESeq: Can you combine explicit and implicit batch effect correction in (SVA+Metadata Variables)?
SVA
batch-effect
DESeq2
R
updated 2 days ago by
Ram
43k • written 5.7 years ago by
Kristin Muench
▴ 620
10
votes
5
replies
1.4k
views
6 follow
Courses in RNAseq data
RNA-seq
Courses
updated 17 days ago by
Ram
43k • written 2.5 years ago by
margot.27.muller
▴ 30
10
votes
6
replies
14k
views
7 follow
How To Identify The 16S Rrna Gene Sequence In A Genome
updated 1 day ago by
colindaven
6.3k • written 10.9 years ago by
Ontheway
▴ 10
1,000 results • Page
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Recent Votes
Answer: Duplicate marking, read names, and the SRA
Comment: Installing/switching between versions of R/Rstudio/Bioconductor
Comment: Installing/switching between versions of R/Rstudio/Bioconductor
Comment: Installing/switching between versions of R/Rstudio/Bioconductor
Answer: Cellranger count error: Input FASTQ file ended prematurely
Comment: Load a full GFF3 into annotation track using arrow (Apollo)
Answer: ClusterProfiler enrichKEGG – remove organism name in plots?
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Comment: Duplicate marking, read names, and the SRA
by
Luka
• 0
>In many cases those remain recoverable using the -F option when you dump the data (cloud download not required) The -F option (or any equ…
Answer: Duplicate marking, read names, and the SRA
by
GenoMax
141k
To be specific, not every dataset in SRA loses the original identifiers. In many cases those remain recoverable using the `-F` option when …
Comment: Installing/switching between versions of R/Rstudio/Bioconductor on personal mach
by
Ram
43k
To use different versions of R and bioconductor, you don't really need RStudio. One can simply create new conda environments with the requi…
Comment: Installing/switching between versions of R/Rstudio/Bioconductor on personal mach
by
BioinfGuru
★ 1.7k
Done. Thank you.
Comment: Installing/switching between versions of R/Rstudio/Bioconductor
by
GenoMax
141k
Perhaps add the word linux/ubuntu to the title? You have noted that the instructions are for Ubuntu in text.
Comment: Installing/switching between versions of R/Rstudio/Bioconductor
by
BioinfGuru
★ 1.7k
Thanks @Ram I'll add that to the intro. I'm the admin on my machine so I haven't found it necessary to install any other way. I'll try a fr…
Comment: Downloading the raw microarray data from GEO
by
GenoMax
141k
No GPR files with this data unless you ask the submitters for them directly. You can find the matrix of data here: https://ftp.ncbi.nlm.nih…
Comment: Cellranger count error: Input FASTQ file ended prematurely
by
GenoMax
141k
That is odd. Thanks for following-up and providing an explanation.
Answer: Cellranger count error: Input FASTQ file ended prematurely
by
bp22
▴ 70
Dear all, The reason for the error was due to the index (I1) fastq files, for example `SI-GA-A1_1_S1_L001_I1_001.fastq.gz` having a lo…
Comment: Installing/switching between versions of R/Rstudio/Bioconductor
by
Ram
43k
Your tutorial required `sudo` and thus is not doable for a majority of people. Any alternative to `sudo snap`?
Comment: Should I apply doublet detection for each pool seperately?
by
Ram
43k
Do not delete posts that have received feedback,
Comment: QC exclusion of A/T or G/C alleles to avoid strand issues
by
_quantum_girl_
▴ 10
They aren't merging any datasets. Just for reference the paper is this: https://www.nature.com/articles/s41380-022-01674-9#MOESM1. Citing: …
Comment: RNA seq differential expression analysis
by
BioinfGuru
★ 1.7k
https://help.galaxyproject.org/
Answer: RNA seq differential expression analysis
by
Mohamed Abderrahmane
▴ 10
The tool RSEM, which performs transcript quantification for RNA-Seq data, provides FPKM.
Comment: Illumina reads preprocessing best practice for snp calling applications
by
Enrique
• 0
Hi! I'm still interested in the step that you use for the SNP calling. Did you use `trimmomatic` or another tool for trimming before mappin…
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