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818 results • Page 1 of 17
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175
votes
46
replies
18k
views
26 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
18 days ago by Istvan Albert 99k
142
votes
14
replies
167k
views
16 follow
What Is The Sequencing 'Depth' ?
next-gen sequencing
updated 27 days ago by 41k • written 13.7 years ago by 158k
118
votes
31
replies
191k
views
17 follow
Correct Way To Parse A Fasta File In Python
python fasta
updated 24 days ago by 41k • written 13.7 years ago by 11k
100
votes
38
replies
29k
views
34 follow
Forum: List of cloud genomics companies
cloud-genomics
updated 15 days ago by 22k • written 10.1 years ago by ★ 3.6k
96
votes
26
replies
31k
views
15 follow
What Methods Do You Use For In/Del/Snp Calling?
short-read-aligner snp sequencing
updated 27 days ago by 41k • written 13.7 years ago by 158k
80
votes
24
replies
11k
views
18 follow
Which Bioinformatics Journals Do You Follow?
bioinformatics-journal
updated 24 days ago by 41k • written 13.6 years ago by 28k
78
votes
21
replies
35k
views
17 follow
How to know that your RNA-seq is stranded or not?
RNA-Seq
updated 21 days ago by • 0 • written 9.6 years ago by ▴ 650
76
votes
26
replies
112k
views
15 follow
How to count fastq reads
sequence next-gen fastq reads
updated 14 days ago by ★ 2.8k • written 8.6 years ago by ▴ 260
74
votes
32
replies
4.9k
views
12 follow
Tool: ChatGPT optimized for bioinformatics questions
gpt
updated 3 days ago by 41k • written 6 months ago by ▴ 770
74
votes
15
replies
35k
views
8 follow
About Paired-End Sequencing
next-gen-sequencing duplicates
updated 24 days ago by 41k • written 13.6 years ago by 158k
72
votes
27
replies
27k
views
16 follow
Tutorial: How To Separate Illumina Based Strand Specific Rna-Seq Alignments By Strand
RNA-seq
updated 4 days ago by • 0 • written 9.8 years ago by 99k
61
votes
23
replies
54k
views
15 follow
How Can I Know The Length Of Mapped Reads From Bam File?
bam mapping
updated 16 days ago by ★ 1.2k • written 10.8 years ago by ★ 1.3k
58
votes
30
replies
11k
views
13 follow
Content Management Systems For Bioinformatics Websites
web
updated 24 days ago by 41k • written 13.7 years ago by 18k
52
votes
14
replies
40k
views
15 follow
Converting Bam To Fastq
next-gen-sequencing fastq
updated 24 days ago by 158k • written 13.6 years ago by ▴ 660
48
votes
21
replies
4.8k
views
9 follow
What Online Gene-Centric Resources Do You Use?
gene database
updated 24 days ago by 41k • written 13.6 years ago by 4.9k
48
votes
18
replies
31k
views
10 follow
How To Get Promoter Sequences For Human Genes?
promoter sequence human genome
updated 24 days ago by 41k • written 13.6 years ago by ★ 1.6k
43
votes
28
replies
5.2k
views
13 follow
Post Your Prefered Bioinformatics Short Code
code
updated 27 days ago by 41k • written 13.7 years ago by 11k
43
votes
21
replies
5.2k
views
9 follow
Helping Biostar Grow
biostars
updated 27 days ago by 41k • written 13.7 years ago by 11k
39
votes
18
replies
5.7k
views
10 follow
Any Open Notebook Science In Bioinformatics?
open-notebook
updated 24 days ago by 41k • written 13.6 years ago by ▴ 380
37
votes
20
replies
6.8k
views
14 follow
Online Bioinformatics Resources
online-resources
updated 24 days ago by 41k • written 13.7 years ago by 11k
35
votes
10
replies
15k
views
11 follow
Which Chip Seq Peak Callers Do You Use?
chip-seq peak-calling
updated 24 days ago by 41k • written 13.7 years ago by ★ 1.4k
32
votes
4
replies
9.2k
views
Fastq Qualities For Solexa/Illumina
sequencing-quality next-gen-sequencing fastq
updated 27 days ago by 41k • written 13.7 years ago by 158k
31
votes
11
replies
5.5k
views
6 follow
Assessing Cluster Reliability/Stability In Microarray Experiments
clustering microarray gene
updated 27 days ago by 41k • written 13.7 years ago by ★ 2.2k
31
votes
8
replies
12k
views
Experiences With Clc Genomics Workbench (Or Other Commercial Tools) For Next-Gen Sequencing
next-gen-sequencing
updated 24 days ago by 41k • written 13.6 years ago by 99k
30
votes
6
replies
13k
views
What Are Some Good Free Bioinformatics And Computational Biology Books?
books online
updated 24 days ago by 41k • written 13.6 years ago by ★ 1.0k
28
votes
13
replies
14k
views
7 follow
Create Consensus Sequences For Sequence Pairs Within A Multiple Alignment?
consensus phylogenetics fasta multiple-alignment
updated 27 days ago by 41k • written 13.7 years ago by ★ 2.0k
28
votes
27
replies
14k
views
9 follow
Extracting A Subset Of Sequences From A Fastq File (Biopython Speed)
biopython python fastq next-gen-sequencing
updated 27 days ago by 41k • written 13.7 years ago by 4.5k
27
votes
4
replies
2.5k
views
How Should A Departing Programmer Organize Things For Successors?
software
updated 24 days ago by 41k • written 13.6 years ago by ▴ 330
27
votes
10
replies
4.2k
views
7 follow
Which Are All The Databases For Annotations Of Biological Pathways?
pathway database annotation
updated 27 days ago by 41k • written 13.7 years ago by 28k
24
votes
9
replies
10k
views
6 follow
Exploring Snp Data
snp association
updated 24 days ago by 41k • written 13.6 years ago by ★ 1.1k
23
votes
21
replies
7.7k
views
9 follow
fastest UMAP method
UMAP R
updated 23 days ago by ★ 25k • written 4.1 years ago by ▴ 30
23
votes
9
replies
6.9k
views
What 3Rd Generation Sequencing Methods Are Out There
next-gen-sequencing
updated 27 days ago by 41k • written 13.7 years ago by 99k
22
votes
21
replies
4.4k
views
7 follow
Quick Programming Challenge: Calculate Common And Unique Regions From A List Of Chromosome Segments
programming
updated 24 days ago by 41k • written 13.6 years ago by 18k
22
votes
8
replies
8.5k
views
7 follow
Gene Expression: Clustering Co-Expressed Genes
gene clustering microarray
updated 27 days ago by 41k • written 13.7 years ago by 11k
22
votes
7
replies
24k
views
What Does The "N50" Mean?
assembly
updated 24 days ago by 41k • written 13.7 years ago by ★ 1.1k
21
votes
13
replies
10.0k
views
8 follow
Blast Motif With Regular Expression ?
blast motif
updated 24 days ago by 41k • written 13.6 years ago by ▴ 80
21
votes
11
replies
2.0k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 16 days ago by 22k • written 9 months ago by ▴ 140
17
votes
4
replies
5.9k
views
Artemis, Apollo Or Anything For Genome Annotation Editing With Next Gen Sequence Data
genome next-gen-sequencing annotation
updated 24 days ago by 41k • written 13.6 years ago by 4.5k
17
votes
14
replies
13k
views
10 follow
Converting Ab1 Trace Files Into Scf Trace Files
sequence
updated 27 days ago by 41k • written 13.7 years ago by 4.5k
17
votes
3
replies
2.7k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
17 days ago by Ram 41k
17
votes
11
replies
3.6k
views
Which Is The Most Accurate Method To Align Multiple Nucleotide Sequences Without Prior Information?
alignment sequence
updated 24 days ago by 41k • written 13.7 years ago by ★ 1.8k
16
votes
4
replies
10k
views
Do P-Values From A Generalised Linear Model Need Correction For Multiple Testing?
statistics r multiple
updated 27 days ago by 41k • written 13.7 years ago by ★ 1.1k
16
votes
3
replies
4.3k
views
A Grep For Gene Ontology ?
gene GO
updated 27 days ago by 41k • written 13.7 years ago by 158k
16
votes
5
replies
12k
views
Where Can I Find The Basepair Positions Of Chromosome Bands?
chromosome position
updated 6 days ago by 19k • written 13.2 years ago by ▴ 370
16
votes
13
replies
5.3k
views
Som For Detection Matlab
disease matlab
updated 24 days ago by 41k • written 13.6 years ago by ▴ 110
15
votes
16
replies
4.6k
views
Gene Expression Experiment Using Ngs Data
next-gen-sequencing gene-expression
updated 24 days ago by 41k • written 13.6 years ago by 11k
15
votes
20
replies
1.1k
views
Bbtools callvariant multisample mode, + base recalibration
Bbtools
14 days ago by Axzd ▴ 50
14
votes
6
replies
6.0k
views
What Tool Should I Use To Assemble Data Produced By A 454 Sequencer?
assembly next-gen-sequencing
updated 24 days ago by 41k • written 13.6 years ago by ▴ 80
14
votes
1
reply
10k
views
From A Geo Gsm Id, How To Obtain The Corresponding Raw File(S) Hosted On Sra?
fastq r bioconductor sra geo
updated 24 days ago by 41k • written 13.6 years ago by ▴ 190
14
votes
10
replies
3.4k
views
6 follow
Should I remove Kmers identified in FastQC?
Illumina fastqc NGS
updated 18 days ago by 136k • written 7.8 years ago by ▴ 30
818 results • Page 1 of 17
Recent Votes
Comment: gatk SelectVariants is giving dupilicate allele error while extracting SNPs out
Answer: How to query 1000 genomes project VCF files for specific regions without downloa
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Answer: Issue softclipping reads when they belong and don't belong to a common amplicon
Answer: how to remove multiple columns from a file in R
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Recent Replies
Comment: SQL request from NCBI metadata and stat_analysis tables by marie.harmel • 0
Thank you for your answer but unfortunately I tried it and it makes the same kind of ouptut as mine, with multtiple new (false) lines (spl…
Comment: Normalization of RNA captureSeq data (<20 genes captured) by ATpoint 78k
What is the question exactly? With only 20 genes you basically **must** use the spike-ins as that few genes do not really allow to normaliz…
Comment: Building reference dbSNP file using WGS samples by analyst ▴ 10
Dear [Brian][1] I realized that problem is with callvariants.sh script because when I called variants using freebayes I did not find aforem…
Comment: gatk SelectVariants is giving dupilicate allele error while extracting SNPs out by analyst ▴ 10
Its bbmap tool callvariants.sh. I think you are right because I did not get such error when I called variants through freebayes.
Answer: Efficient way of calculating the number of mismatches between two sets of sequen by j.gleixner • 0
Since all your sequences have the same length (say M) and you are only looking concerned with hamming distance you can encode your sequence…
Comment: Where to download excel file to include major human disease, prevalence and inci by GenoMax 136k
ChatGPT says Disease Prevalence Approximate Number of Cases (Worldwide) Cardiovascular Diseases High Over 17 million deaths annual…
Comment: Where to download excel file to include major human disease, prevalence and inci by Pierre Lindenbaum 158k
using wikidata: https://query.wikidata.org/sparql?query=%0ASELECT%20%3Fitem%20%3FitemLabel%20%3FitemDescription%20%3Fprevalence%20%3Fdoid%0…
Comment: Opinion on miRNA pipeline by ju_ra • 0
Thank you very much! I see you decided to go for Bowtie as aligner. Do you see any issue in using STAR (as a splice aware reader technicall…
Answer: How to avoid missannotated GO terms? by Elisabeth Gasteiger ★ 2.3k
Please contact the UniProt helpdesk whenever you find such annotations, especially in these cases where the GO evidence/source tag says "Un…
Comment: gatk SelectVariants is giving dupilicate allele error while extracting SNPs out by Pierre Lindenbaum 158k
again, it's a problem with the variant caller, which is ?
Comment: gatk SelectVariants is giving dupilicate allele error while extracting SNPs out by analyst ▴ 10
Not only opposite case but same case nucleotides are also present in vcf fle. ![enter image description here][1] [1]: /media/images/4…
Comment: gatk SelectVariants is giving dupilicate allele error while extracting SNPs out by analyst ▴ 10
Yes [Pierre][1] there is lower case **g** in **REF** and upper case **G** in **ALT** field. My question is that why variant caller is calli…
Answer: gatk SelectVariants is giving error while extracting SNPs out of vcf file by Pierre Lindenbaum 158k
This is an error in your input vcf file. The REF allele MUST be différent from the ALT allele.
Comment: gatk SelectVariants is giving error while extracting SNPs out of vcf file by analyst ▴ 10
I observed that my reference genomic fasta file contains both lower case and upper case nucleotides. Do I need to convert lower case nucle…
Comment: Building reference dbSNP file using WGS samples by analyst ▴ 10
I observed that both lower and upper cases are present in reference fasta file. Is bbtool case sensitive that while calling variants it con…
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