Limit : this month
all time today this week this month this year
1,000 results • Page 2 of 20
Sort: Votes
Rank Views Votes Replies
57
votes
43
replies
14k
views
8 follow
Forum: Best RNA-Seq aligner: A comparison of mapping tools
NGS alignment genome RNA-Seq sequence
updated 17 days ago by 38k • written 4.2 years ago by 9.9k
50
votes
14
replies
27k
views
8 follow
Where To Download Pam50 Gene Set?
cancer annotation classification
updated 27 days ago by 38k • written 9.7 years ago by ▴ 930
50
votes
20
replies
4.9k
views
18 follow
Forum: Explain bioinformatics to a 10 year old
bioinformatics
updated 17 days ago by 38k • written 7.1 years ago by ★ 1.1k
50
votes
3
replies
4.0k
views
Tutorial: [[ Please read before posting a question ::: How To Ask A Good Question ]]
how-to
updated 12 days ago by 38k • written 4.2 years ago by 70k
49
votes
22
replies
18k
views
7 follow
Batch correction in DESeq2
DESeq2 Batch Effect Combat
updated 20 days ago by 84k • written 3.4 years ago by ▴ 470
48
votes
19
replies
49k
views
11 follow
How Can I Convert Bam/Sam To Wiggle
wiggle perl samtools
updated 18 days ago by 38k • written 12.5 years ago by ★ 1.4k
47
votes
11
replies
3.6k
views
Forum: What Operating System Are You Using For Bioinformatic Work?
linux windows
updated 25 days ago by 38k • written 9.3 years ago by 11k
47
votes
4
replies
9.8k
views
How do experienced people look for full reference genomes?
reference-genome fasta
updated 2 days ago by 38k • written 8.8 years ago by ▴ 320
47
votes
37
replies
7.3k
views
12 follow
Forum: The Organisation Of Really Contrived Acronyms (Orca)
acronyms
updated 27 days ago by 38k • written 9.6 years ago by 2.3k
46
votes
9
replies
7.8k
views
10 follow
Forum: Bioinformatics For Clinicians - What To Teach Them?
education
updated 25 days ago by 38k • written 9.3 years ago by ▴ 140
45
votes
14
replies
58k
views
10 follow
Tutorial: Using R in Conda
R
updated 12 days ago by 38k • written 2.7 years ago by ▴ 690
45
votes
15
replies
25k
views
15 follow
How To Do Normalization Of Two Chip-Seq Data?
chip-seq
updated 27 days ago by • 0 • written 11.0 years ago by ▴ 200
44
votes
11
replies
4.8k
views
7 follow
Forum: Asking The Developers Before Asking Biostars
meta
updated 26 days ago by 38k • written 9.5 years ago by ▴ 850
43
votes
6
replies
11k
views
Tutorial: Annotating TCGA MAFs with the latest Ensembl/Gencode transcripts
ensembl maf tcga vcf
updated 24 days ago by 38k • written 9.2 years ago by 5.9k
42
votes
21
replies
14k
views
10 follow
Forum: A Database Of Signatures Of Selection In The 1000 Genomes Dataset
1000genomes human selection
updated 23 days ago by 38k • written 9.1 years ago by 27k
42
votes
19
replies
5.9k
views
7 follow
Makefile-Driven Workflows And Bioconductor Objects
r bioconductor workflow pipeline
updated 4 days ago by 38k • written 10.1 years ago by ▴ 170
42
votes
33
replies
6.8k
views
8 follow
Tutorial: How to Use Biostars, Part-I: Questions, Answers, Comments and Replies
meta how-to documentation
12 days ago by Ram 38k
42
votes
15
replies
15k
views
13 follow
How To Blast A Sequence Against Multiple Databases
blast
updated 11 days ago by • 0 • written 11.5 years ago by ▴ 50
40
votes
24
replies
2.1k
views
13 follow
Forum: What programming language do you use the most in your work? Which one do you like the best?
Programming Python R Scripting
updated 17 days ago by 38k • written 2.1 years ago by ▴ 80
40
votes
9
replies
10k
views
10 follow
Tutorial: Resources for flow cytometry bioinformatics
single-cell flow-cytometry
updated 20 days ago by 38k • written 9.0 years ago by ★ 1.9k
40
votes
11
replies
6.4k
views
9 follow
Forum: "An Explosion Of Bioinformatics Careers" - from Science Careers
career
updated 20 days ago by 38k • written 8.8 years ago by 3.2k
37
votes
13
replies
3.1k
views
6 follow
Forum: Bringing Back Downvote
meta
updated 27 days ago by 38k • written 9.8 years ago by 33k
37
votes
8
replies
9.2k
views
10 follow
Tutorial: Methylation Analysis Tutorial in R_part1
cancer R methylation
updated 17 days ago by 38k • written 2.5 years ago by 2.9k
36
votes
21
replies
16k
views
9 follow
Forum: What Is Your Favorite Conserved Protein?
protein conservation
updated 27 days ago by 38k • written 9.6 years ago by 11k
36
votes
2
replies
5.7k
views
Forum: Brian Haas of the Trinity Assembler uses this
uses-this
updated 17 days ago by 38k • written 8.4 years ago by 97k
35
votes
10
replies
3.8k
views
7 follow
Why There Are A Lot Of Bioinformatic Tools Implemented In Python?
python java
updated 27 days ago by 38k • written 9.7 years ago by ▴ 50
34
votes
30
replies
1.8k
views
6 follow
Tool: BioFlows - Container-enabled Bioinformatics pipeline engine
workflow next-gen RNA-Seq pipeline
updated 5 days ago by 38k • written 2.3 years ago by ▴ 90
34
votes
13
replies
5.6k
views
11 follow
Forum: Best Way To Learn Bioinformatics For System Level Programmers.
NGS HPC
updated 24 days ago by 38k • written 9.2 years ago by ▴ 90
34
votes
15
replies
5.0k
views
6 follow
Tutorial: How to do data cleaning for VCF genetic file
vcf
updated 12 days ago by 38k • written 3.8 years ago by ★ 9.3k
34
votes
15
replies
18k
views
12 follow
Tutorial: TCGA UUIDS to TCGA barcode (SampleID) in R
next-gen GDC R TCGA
updated 11 days ago by 38k • written 6.5 years ago by ▴ 310
33
votes
16
replies
15k
views
10 follow
Tutorial: Machine Learning For Cancer Classification - Part 3 - Predicting With A Random Forest Classifier
machine-learning classification
updated 25 days ago by 38k • written 9.3 years ago by 20k
33
votes
16
replies
4.4k
views
9 follow
If you have a chance to ask questions to the CEO of Illumina, what would you do?
illumina
updated 20 days ago by 38k • written 8.9 years ago by ▴ 60
33
votes
17
replies
2.7k
views
7 follow
Forum: What bioinformatics tools/software do not get enough recognition?
software
updated 17 days ago by 38k • written 8.4 years ago by 24k
32
votes
14
replies
6.1k
views
9 follow
Forum: Pat Schloss of Mothur uses this
uses-this
updated 17 days ago by 38k • written 8.4 years ago by 97k
32
votes
6
replies
12k
views
7 follow
News: Ncbi Releases Entrez Direct, The Entrez Utilities On The Unix Command Line
ncbi genbank pubmed command-line entrez
updated 24 days ago by 38k • written 9.1 years ago by 153k
32
votes
5
replies
5.2k
views
Forum: ipython notebooks on bioinformatics and genomics
python
updated 4 days ago by 38k • written 7.7 years ago by ★ 2.1k
32
votes
4
replies
6.4k
views
Tutorial: Fast LD computation from VCF files using tomahawk
ld snp vcf vcftools bcftools
updated 9 days ago by 38k • written 4.4 years ago by ★ 1.5k
31
votes
12
replies
26k
views
7 follow
Tutorial: So What Does The Sequence Duplication Rate Really Mean In A Fastqc Report
quality-control fastqc
updated 26 days ago by 38k • written 9.4 years ago by 97k
31
votes
9
replies
24k
views
11 follow
Tutorial: installing samtools with conda - overcoming two common errors
samtools conda
updated 12 days ago by 38k • written 2.6 years ago by ▴ 140
31
votes
6
replies
8.7k
views
Tutorial: A simple tutorial for a complex ComplexHeatmap
clustering pam arthritis ComplexHeatmap
updated 12 days ago by 38k • written 2.8 years ago by 84k
31
votes
8
replies
3.5k
views
6 follow
Forum: Getting Things Done In Bioinformatics (More I Know, Less I Do?)
literature analysis
updated 25 days ago by 38k • written 9.4 years ago by 3.2k
30
votes
7
replies
15k
views
8 follow
RNA-Seq analysis using STAR and Salmon
RNA-Seq
updated 26 days ago by ▴ 30 • written 5.5 years ago by ▴ 210
30
votes
13
replies
6.4k
views
6 follow
Bioinformatics: How To Version Control Small Scripts Located All Over The Server.
scripts version-control
updated 26 days ago by 38k • written 9.5 years ago by ▴ 720
30
votes
8
replies
10k
views
7 follow
Tutorial: How To Create A Bioinformatics Pipeline Using Spotify !
python pipeline
updated 20 days ago by 38k • written 9.0 years ago by ▴ 800
29
votes
5
replies
5.9k
views
Tutorial: Gnu Parallel Tutorial
parallel gnu-parallel
updated 26 days ago by 38k • written 9.5 years ago by ★ 4.3k
29
votes
4
replies
6.6k
views
Forum: Update to tbb (2021.1.1, conda-forge) breaks bowtie2 (bioconda)
software-error bowtie2 bioconda conda tbb
updated 17 days ago by 38k • written 2.1 years ago by ▴ 240
29
votes
10
replies
5.1k
views
10 follow
Forum: Interesting/useful/beautiful bio sequence viz tools
viz python visualisation
updated 17 days ago by 38k • written 5.0 years ago by 20k
29
votes
14
replies
7.9k
views
7 follow
How To Download All The Introns From Ensembl
ensembl fasta intron
updated 2 days ago by 38k • written 11.6 years ago by ★ 2.9k
29
votes
16
replies
2.6k
views
Forum: Making bioinformatics help and tutorials open
ensembl
updated 18 days ago by 38k • written 8.6 years ago by 23k
28
votes
15
replies
4.0k
views
8 follow
Forum: Anyone Use R For Their Bioinformatics Work?
r
updated 26 days ago by 38k • written 9.4 years ago by ▴ 310
1,000 results • Page 2 of 20
Recent Votes
A: circos plot gene expression by RNA-Seq
Create Interval List for Picard CollectRNASeqmetrics
Answer: multiallelic variants
Answer: LIMMA calculates identical adj.P.Val for different proteins from proteomics
Comment: Hard clip fastq
Answer: Hard clip fastq
Answer: Hard clip fastq
Recent Locations • All
France, just now
Hong Kong, 1 minute ago
Sweden, 1 minute ago
South Korea, 1 minute ago
Manipal School of Life Science, 1 minute ago
Poland, 7 minutes ago
United States, 8 minutes ago
Recent Awards • All
Popular Question to seta ★ 1.8k
Popular Question to Lluís R. ★ 1.2k
Popular Question to sharmatina189059 ▴ 110
Popular Question to ahnje770 ▴ 20
Popular Question to Gjain 5.7k
Popular Question to mark.rose ▴ 50
Scholar to ATpoint 70k
Recent Replies
Answer: getTable ignores query ranges by Kyle • 0
The short answer is you can theoretically do this you just need to a) make your SNPs are two base-pairs in length (i.e. add 1 to the end) a…
Answer: Installing rlang version 1.1.0 by Lluís R. ★ 1.2k
Having a package duplicated means they are at a different `.libPaths()` sites. Check which version is in which directory and directly remov…
Answer: Somatic truth set by samuel ▴ 180
I think I have found an naswer to my own question. Should anyone have the same question as of March 2023 the relevant data and information…
Answer: GetBM - GERP conservation score for GRCm39 by Kyle • 0
Final solution thanks to Ben's help:: # convert df to Grange. snp_gr <-makeGRangesFromDataFrame(BM_SNPs, seqnames.field = "seqnam…
Comment: GetBM - GERP conservation score for GRCm39 by Kyle • 0
Perfect answer, thanks Ben!
Answer: MapSplice2 gives error if the thread count (-p value) is greater than 2 by vishal.koparde ▴ 10
Any updates??
Comment: how to know what adapter sequences to trim for RNA-seq? by lunarskye222 • 0
Do you happen to know if the middle part of this udi barcode (GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTACATATCTCG) is also part of the TruSeq a…
Comment: Hard clip fastq by GenoMax 127k
If you simply want the reads to be compatible in length then do not adapter trim.
Comment: Hard clip fastq by samuel ▴ 180
@ATpoint, so no adapter trimming first? Just trim the raw fastq?
Answer: Confused on strandedness, what are antisense transcripts? by dsull ★ 4.0k
Think of it on the DNA level: If the mRNA made is the same sequence as the "sense" DNA strand (except U's instead of T's), it is a "sense" …
Comment: Protein name in GenBank by GenoMax 127k
If you must do this programmatically then collect all cases where things differ in order of words (or number of words) like the example abo…
Answer: Hard clip fastq by GenoMax 127k
You can also use `reformat.sh` from [**BBMap suite**][1] as follows reformat.sh -Xmx2g in1=R1.fq.gz in2=R2.fq.gz out1=R1_trim.fq.gz …
Answer: Hard clip fastq by ATpoint 70k
Use `seqtk trimfq`. It makes sense to trim first to your desired length and then run identical downstream processing for a fair comparison.…
Comment: Protein name in GenBank by Mensur Dlakic ★ 23k
I think you either take time to do it properly, or you will end up missing some proteins. BLAST sequence search, even on 1500 genomes, can …
Comment: Log2 FC Threshold by ATpoint 70k
Since OP is using GEO2R which uses limma the appropriate function (need to run manually, GEO2R does not support) is `limma::treat()` rather…
Traffic: 1942 users visited in the last hour
Content Search
Users
Tags
Badges
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6