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1,000 results • Page
2 of 20
Sort: Views
Rank
Views
Votes
Replies
79
votes
35
replies
8.1k
views
17 follow
Forum:
Do bioinformaticians often break molecular biologists' hearts by being the first author?
authorship
career
updated 9 days ago by
jli.ww
• 0 • written 7.6 years ago by
sentausa
▴ 650
15
votes
10
replies
8.0k
views
Time course experiments in DESeq2
HTSeq
DESeq2
time-course
RNA-seq
updated 7 days ago by
Ram
39k • written 7.1 years ago by
Chris Gene
▴ 80
3
votes
3
replies
8.0k
views
Tutorial:
High Speed Downloading of SRA, SAM and Fastq Files
fastq
sam
Aspera-Connect
sra
updated 19 days ago by
Ram
39k • written 5.5 years ago by
Wenhu_Cao
▴ 100
13
votes
4
replies
7.9k
views
Selection of a "suggestive" level of association in a GWAS study
association
GWAS
statistics
5 days ago by
Vincent Laufer
★ 2.9k
2
votes
2
replies
7.9k
views
Tutorial:
Use fastp to preprocess FASTQ data with unique molecular identifer (UMI) integrated
FASTQ
UMI
fastp
updated 20 days ago by
Ram
39k • written 5.5 years ago by
chen
★ 2.4k
10
votes
1
reply
7.7k
views
Tutorial:
How to select reads with specific flag using samtools
next-gen-sequencing
updated 21 days ago by
Ram
39k • written 5.8 years ago by
imlituan
▴ 110
0
votes
0
replies
7.6k
views
Tutorial:
Multicollinearity VIF (Variance Inflation Factor) Analysis in Panel data EVIEWS 9
R
eviews
updated 19 days ago by
Ram
39k • written 5.4 years ago by
helgasaraswati
▴ 10
11
votes
2
replies
7.4k
views
Tutorial:
Plotting the coverage and depth (Y-axis) statistics of a bam file along the genome (X-axis), using Samtools, awk and R.
genome
plot
coverage
samtools
updated 26 days ago by
Ram
39k • written 6.2 years ago by
kirannbishwa01
★ 1.5k
30
votes
19
replies
7.4k
views
6 follow
Forum:
Programming language use distribution from recent programs / articles
programming-language
programs
updated 25 days ago by
Ram
39k • written 6.1 years ago by
Eric Normandeau
11k
45
votes
28
replies
7.4k
views
9 follow
Tutorial:
How to Use Biostars, Part-I: Questions, Answers, Comments and Replies
documentation
meta
how-to
19 days ago by
Ram
39k
11
votes
7
replies
7.3k
views
Tutorial:
ClusterProfiler A software for functional enrichment of differentially expressed genes- A tutorial
clusterProfiler
Software
rna-seq
updated 18 days ago by
Ram
39k • written 2.3 years ago by
Novogene
▴ 300
7
votes
17
replies
7.3k
views
10 follow
Python FASTA scripting
Python
FASTA
updated 1 day ago by
Ram
39k • written 3.7 years ago by
damonlbp
▴ 20
20
votes
2
replies
7.2k
views
Tutorial:
Creating a karyotype plot with genes using karyoploteR
karyoploteR
visualization
R
genome
updated 20 days ago by
Ram
39k • written 5.5 years ago by
bernatgel
★ 3.4k
68
votes
48
replies
7.1k
views
16 follow
Forum:
Stranger Things: unexpected limitations of popular tools
samtools
hisat2
updated 25 days ago by
Ram
39k • written 5.9 years ago by
Istvan Albert
98k
22
votes
15
replies
7.0k
views
11 follow
Forum:
RNASeq user experience with Novogene?
RNA-Seq
updated 13 days ago by
Ram
39k • written 4.9 years ago by
ivanarg2
▴ 80
9
votes
9
replies
6.9k
views
News:
NovaSeq quality analysis
sequencing
updated 25 days ago by
Ram
39k • written 6.0 years ago by
Brian Bushnell
18k
16
votes
6
replies
6.9k
views
7 follow
Forum:
What computer skills should I put on my resume?
resume
genome
updated 19 days ago by
Ram
39k • written 5.5 years ago by
Kenny
▴ 30
2
votes
2
replies
6.7k
views
GATK -Xmx4G and -Xmx8G
GATK
HaplotypeCaller
updated 5 days ago by
Ram
39k • written 3.9 years ago by
lovegenetics
▴ 70
12
votes
11
replies
6.7k
views
Tutorial:
How to upload your RNA-Seq data to NCBI Sequence Read Archive (SRA)
NCBI
Bioproject
SRA
BioSample
updated 27 days ago by
Ram
39k • written 6.2 years ago by
CandiceChuDVM
★ 2.4k
1
vote
4
replies
6.6k
views
Calculating Fst between different populations at a specific locus
statistics
updated 22 days ago by
Ram
39k • written 5.9 years ago by
Pinter
• 0
18
votes
6
replies
6.5k
views
VCF header line counting
vcf
header
updated 11 days ago by
beausoleilmo
▴ 530 • written 8.3 years ago by
basalganglia
▴ 40
4
votes
14
replies
6.5k
views
Homer detailed annotation
ChIP-Seq
annotate peaks
HOMER
updated 22 days ago by
yeji
• 0 • written 6.3 years ago by
xiaofeiwang198266
▴ 20
2
votes
0
replies
6.5k
views
Tutorial:
How to - Install Python packages in a virtual environment (virtualenv)
ubuntu
cutadapt
virtualenv
python
windows
updated 19 days ago by
Ram
39k • written 5.5 years ago by
vimalkvn
▴ 310
6
votes
6
replies
6.5k
views
Salmon transcriptome generation
RNA-Seq
updated 11 days ago by
Ram
39k • written 4.2 years ago by
Morris_Chair
▴ 330
6
votes
10
replies
6.5k
views
Average bigwig files (not sum)
ChIP-Seq
bigwig
updated 28 days ago by
vanbelj
▴ 20 • written 3.2 years ago by
srhic
▴ 40
3
votes
1
reply
6.4k
views
News:
LinkedOmics - a web portal for TCGA/CPTAC data
TCGA
cptac
cancer
updated 20 days ago by
Ram
39k • written 5.5 years ago by
GenoMax
129k
6
votes
6
replies
6.4k
views
Significance of Tile in Sequencing?
genomics
updated 12 days ago by
GenoMax
129k • written 2.2 years ago by
joe_genome
▴ 40
15
votes
1
reply
6.4k
views
Tutorial:
A compilation of conversion tools for BED, SAM/BAM, psl, pslx, blast tabular and blast xml
psl
SAM
blast
BED
updated 20 days ago by
Ram
39k • written 5.6 years ago by
Joseph Hughes
★ 3.0k
14
votes
6
replies
6.2k
views
Heatmap error: 'x' must be a numeric matrix
R
heatmap
updated 22 days ago by
Ram
39k • written 18 months ago by
adnan.sabar7865
▴ 20
5
votes
5
replies
6.1k
views
Tutorial:
use firehose_get to download TCGA data
tcga
RNA-Seq
updated 20 days ago by
Ram
39k • written 5.6 years ago by
jmzeng1314
▴ 130
7
votes
7
replies
6.1k
views
6 follow
filtering the reads based on the length
RNA-Seq
updated 14 days ago by
geocarvalho
▴ 330 • written 4.7 years ago by
alireza346
▴ 10
14
votes
5
replies
6.1k
views
Forum:
Seurat vs Monocle
R
RNA-Seq
next-gen
single-cell
updated 5 days ago by
Ram
39k • written 3.9 years ago by
Arup Ghosh
3.2k
24
votes
21
replies
6.1k
views
9 follow
News:
Misunderstood parameter of NCBI BLAST
blast
alignment
updated 12 days ago by
Ram
39k • written 4.7 years ago by
Farbod
★ 3.4k
20
votes
24
replies
6.0k
views
6 follow
Forum:
Python or R
RNA-Seq
alignment
next-gen-sequencing
updated 14 days ago by
Ram
39k • written 5.0 years ago by
Qingyang Xiao
▴ 160
7
votes
1
reply
6.0k
views
Forum:
What are chromatin states ?
genome
ChIP-Seq
updated 18 days ago by
Ram
39k • written 3.6 years ago by
rohitsatyam102
▴ 690
129
votes
38
replies
6.0k
views
21 follow
Forum:
Who are the Greatest Bioinformaticians Of All Time (GBOAT)
bioinformatics
updated 7 days ago by
Ram
39k • written 4.2 years ago by
Istvan Albert
98k
0
votes
8
replies
5.8k
views
Bedtools problem : It looks as though you have less than 3 columns at line: 1. Are you sure your files are tab-delimited
bedtools
fasta
updated 1 day ago by
Michael
• 0 • written 3.6 years ago by
Tomm
• 0
3
votes
2
replies
5.7k
views
Forum:
Best book for learning Next-Generation Sequencing Data Analysis?
ngs
updated 18 days ago by
Ram
39k • written 5.3 years ago by
Abdul Rafay Khan
★ 1.2k
3
votes
3
replies
5.7k
views
How to convert from a .txt to a .maf file?
maf
R
txt
TCGA
updated 15 days ago by
Ram
39k • written 5.2 years ago by
ksanchez1296
• 0
7
votes
4
replies
5.7k
views
Best/right way to quantify small RNA transcripts
small RNA-seq
RPM
multi-mapping
updated 19 days ago by
Sergio
• 0 • written 5.3 years ago by
Chirag Nepal
★ 2.3k
1
vote
7
replies
5.6k
views
Human reference files in HG38 GATK resource bundle
VCF
updated 7 days ago by
dare_devil
★ 3.1k • written 5.4 years ago by
win
▴ 940
6
votes
6
replies
5.6k
views
6 follow
How To Retrieve Data From Jgi Automatically Given A Set Of Ids?
r
python
api
xml
updated 26 days ago by
GenoMax
129k • written 9.5 years ago by
Manu Prestat
4.1k
11
votes
5
replies
5.5k
views
Forum:
Slurm, Son of Grid Engine, Mesos, and Hadoop YARN vs HTCondor and Torque
PBS
HPC
SDSC
Torque
updated 13 days ago by
Ram
39k • written 4.8 years ago by
Shicheng Guo
★ 9.3k
18
votes
2
replies
5.5k
views
Tutorial:
Update kraken databases
database
kraken
k-mer
python
updated 25 days ago by
Ram
39k • written 6.1 years ago by
Sej Modha
5.2k
18
votes
3
replies
5.5k
views
How to process (seems) Agilent microarrry data?
microarray
rna
updated 10 days ago by
survive
• 0 • written 3.9 years ago by
MatthewP
★ 1.2k
12
votes
13
replies
5.5k
views
9 follow
Mauve installation fails in Windows 8
software error
alignment
updated 22 days ago by
Neonny86
• 0 • written 6.8 years ago by
Bioblazer
▴ 50
5
votes
10
replies
5.4k
views
7 follow
Time Course RNA-Seq
Time-Course
Bacteria
RNA-Seq
updated 7 days ago by
Ram
39k • written 6.7 years ago by
buthercup_ch
▴ 30
6
votes
7
replies
5.4k
views
6 follow
Forum:
Bioinformatic or bioinformatics?
spelling
updated 20 days ago by
Ram
39k • written 5.7 years ago by
jon.brate
▴ 290
14
votes
11
replies
5.4k
views
7 follow
Forum:
Do you need a deduplication tool for FASTQ data in fastp?
deduplication
fastp
updated 6 days ago by
Ram
39k • written 4.0 years ago by
chen
★ 2.4k
4
votes
11
replies
5.4k
views
7 follow
GeneBank accession 2 Entrez gene id
NCBI
Genbank
updated 29 days ago by
Dast0840
• 0 • written 7.6 years ago by
盛夏
▴ 10
1,000 results • Page
2 of 20
Recent Votes
A: 1/2 in VCF genotype field?
1/2 in VCF genotype field?
C: How To Fix A Bam-Readcount Sm Error: "Couldn'T Grab Single-End Mapping Quality F
C: Regarding filter primary alignment
Alternative approach to rarefying in 16S rRNA analysis
Answer: Gene prediction software
Comment: retaining only the clusters of interest
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Recent Replies
Comment: calculate p value and associated z score for snp-gene pair
by
rheab1230
▴ 140
Thank you so much for the information.
Comment: Gviz Coverage Plots
by
Ram
39k
I understand that but stick to one place at least for a while and always link to other places you've posted (reasonable places, we don't ne…
Comment: Gviz Coverage Plots
by
Researcher
• 0
Sorry, I didn't know that posting it on another forum was rude but I really need help with this.
Comment: Correct way to remove Nextera adapters from ITS sequences
by
mattze731
▴ 10
Trimmomatic allows to enter custom adapter sequences, but I don't see how this would change the outcome. Similarly, trimmomatic also allows…
Comment: phage genome submission in ncbi genebank
by
acvill
▴ 290
When running prokka, did you call the `--compliant` parameter to enforce Genbank compliance? https://github.com/tseemann/prokka#ncbi-genban…
Comment: Correct way to remove Nextera adapters from ITS sequences
by
Trivas
★ 1.2k
Some of these programs have a minimum size threshold for a read to be retained. You could play around with that to keep all trimmed reads 5…
Comment: retaining only the clusters of interest
by
seidel
11k
Could it be that idents needs a character string instead of integers? Also, you have an extra comma in your call after c(0:16).
Comment: retaining only the clusters of interest
by
Ram
39k
`idents` is probably a factor so even though you've removed cells with those level values, the factor level still remains. Don't worry abou…
Comment: Contigs to chromosomes annotation
by
GenoMax
129k
I would still recommend using `minimap2` and aligning against reference. It should go pretty fast. You can either work with PAF (default) f…
Comment: Diff Bind Questions
by
Ram
39k
I've moved this to a comment as it does not directly answer OP's question.
Comment: Diff Bind Questions
by
Daniel
▴ 30
I'm very new myself and there's plenty of people who can answer your question better than I can, but here is a resource I found extremely h…
Comment: Contigs to chromosomes annotation
by
alexandru.bologa.marian
▴ 50
If the number of contigs was so small I would manually check each alignment and edit the header of each contig,inserting the corresponding …
Comment: Gviz Coverage Plots
by
Ram
39k
Do not post on multiple fora just because you want a fast response, it's plain rude. At least point to the other post so we know you're ask…
Comment: Limma returned only positive logFC values
by
melissachua90
▴ 40
No the library sizes across conditions should be relatively homogenous because it's from the same dataset. I clustered the samples into sub…
Comment: Limma returned only positive logFC values
by
LChart
2.6k
It shouldn't, unless there's a misspecification in design. Is it the case that the library sizes are very different between the conditions …
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