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315
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90
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220
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28 follow
updated 4 hours ago by
Ram
37k
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written 9.6 years ago by
Malachi Griffith
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193
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44
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66k
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33 follow
updated 6 days ago by
Ram
37k
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written 10.0 years ago by
Obi Griffith
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182
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50
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41 follow
updated 3 hours ago by
Ram
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written 11.9 years ago by
Zhangleisdau
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105
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updated 3 hours ago by
Ram
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written 9.7 years ago by
henryvuong
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updated 1 day ago by
Ram
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written 7.1 years ago by
Ishtiaq Ahmad Khan
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43
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8
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33k
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updated 17 hours ago by
Asan Emirsale
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written 5.8 years ago by
Abdul Rafay Khan
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10
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33k
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updated 6 days ago by
Ram
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written 11.6 years ago by
Ketan Padiya
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89
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19
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30k
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updated 5 hours ago by
Ram
37k
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written 8.9 years ago by
Obi Griffith
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3
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29
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20k
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updated 5 days ago by
Ram
37k
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written 10.4 years ago by
Istvan Albert
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77
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22
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20k
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14 follow
updated 4 hours ago by
Ram
37k
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written 10.3 years ago by
Prakki Rama
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2.6k
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14
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13 follow
7
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written 11.1 years ago by
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14k
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23
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18
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14k
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written 14 months ago by
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written 8.8 years ago by
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13k
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updated 2 days ago by
Ram
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written 7.1 years ago by
Jeroen Van Goey
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28
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6
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13k
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updated 6 days ago by
ATpoint
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written 5.0 years ago by
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updated 1 day ago by
Ram
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written 7.2 years ago by
colin.kern
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22
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Forum:
Nextseq for analysts
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written 7.1 years ago by
Chris Gene
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updated 3 hours ago by
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written 7.1 years ago by
ssukumaran
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updated 2 days ago by
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written 4.4 years ago by
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15
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written 8.7 years ago by
microbeatic
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written 7.1 years ago by
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updated 1 day ago by
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written 7.1 years ago by
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Tutorial:
Informatics of RNA-Seq.
updated 5 days ago by
Ram
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written 7.1 years ago by
GouthamAtla
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updated 5 days ago by
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written 7.1 years ago by
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Recent Replies
Comment: FastQ: quality string length is not equal to sequence length
by
GenoMax
120k
Can you try aligning the data using `minimap2`? This may be a bug in `STARlong`.
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See what the mapping says. Align your data to a reference.
To be honest, your data looks a little suspect, you have too many full adapters…
Comment: What do Over-represented Sequences mean in FastQC using RNA-Seq?
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> A sample wouldnt have two different types of Adapters so this confuses
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One is a primer and other is an adapter. Those are two diff…
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If it can rescue a read it will do so without discarding it, so try realigning now to see if it worked.
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use [the `chunksize` option](https://pandas.pydata.org/pandas-docs/stable/user_guide/io.html#iterating-through-files-chunk-by-chunk) when y…
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It's 20 months late, but today I encountered the same problem. Now, I know this problem is caused by annotation files. I use the new versio…
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Thanks for the reply, I used seqkit sana but all the lines passed and it discarded 0 lines.
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no there are pleny of fast optimal aligners (just that none are suited for large sequences)
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The green circled proteins duplicated in the common ancestor of the species in question, so they are out-paralogs. While the blue circled p…
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![in&out paralogs][1]
This is the schematic diagram of in-paralogs and out-paralogs provided in the lecture. The question only say design …
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Well, what does that entry look like in the fastq?
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find /path/to/root -type f -name "*.db" > out.list
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