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Limit : this week
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208 results • Page
2 of 5
Sort: Views
Rank
Views
Votes
Replies
12
votes
6
replies
479
views
Problematic fastq files...How can we trust them?
fastq
5 days ago by
blackadder
▴ 30
4
votes
6
replies
472
views
From TPM to raw counts
tpm
Deconvolution
rnaseq
4 days ago by
Gama313
▴ 120
0
votes
1
reply
470
views
How to determine E. coli MLST (Multi Locus Sequence Typing ) from the sequencing of the PCR product of 7 housekeeping genes
MLST
phylogenetic
nanopre
updated 1 day ago by
Joe
21k • written 1 day ago by
Ralph
• 0
3
votes
6
replies
468
views
scRNA-seq: Consistent low number of cells and low fraction reads across the samples
scRNA-seq
cells
mRNA
expression
gene
updated 5 days ago by
jv
★ 1.3k • written 7 days ago by
newbee
▴ 40
0
votes
1
reply
450
views
Loftee no splice site annotations
Loftee
VEP
updated 6 days ago by
AMARU
• 0 • written 21 months ago by
Filago
▴ 90
0
votes
4
replies
445
views
Can I use TCGA-LUAD RNAseq count that had already normalized by RSEM in Limma-voom
RNAseq
differential-gene-expession
limma
batch-effect
updated 1 day ago by
Ram
41k • written 5 days ago by
fluke
• 0
3
votes
3
replies
425
views
How to obtain data on the coordinates of the Exon region from UCSC
hg19
UCSC
exon
5 days ago by
ayasu
• 0
3
votes
4
replies
400
views
Is bisulfite-seq data from different studies comparable with each other?
Methylation
updated 4 days ago by
NextGenSeek
▴ 10 • written 7 days ago by
CTLong
▴ 30
0
votes
1
reply
391
views
The number of variations in the pan-genome is reduced compared to the variations in the input VCF file
vg
updated 5 days ago by
Jordan M Eizenga
▴ 410 • written 12 days ago by
Wenke
• 0
0
votes
0
replies
390
views
Job:
Postdoc in comparative genomics of plant-associated bacteria (Polytechnic University of Madrid, SPAIN)
evolution
genomics
mutation
bacteria
genomes
5 days ago by
Alejandro
• 0
5
votes
5
replies
380
views
Using metagenome assembly and binning to identify and mitigate contamination in a genome
genomes
contamination
metagenomics
2 days ago by
btc347
• 0
1
vote
5
replies
373
views
How can I map coordinates between two strains of yeast?
coordinates
yeast
genome
mapping
fasta
6 days ago by
Jimmy
▴ 10
1
vote
1
reply
366
views
Trinity 'genes' too much
Trinity
RNA-seq
updated 1 day ago by
Ram
41k • written 2.1 years ago by
Hou
• 0
2
votes
5
replies
354
views
FastQC without yellow box
fastqc
updated 1 day ago by
Ram
41k • written 2 days ago by
lobelino
• 0
4
votes
3
replies
353
views
GO categorization
Ontology
Gene
updated 5 days ago by
geneontologyhelp
▴ 290 • written 6 days ago by
m.habib
• 0
0
votes
5
replies
344
views
HTseq reports missing attribute name
htseq
updated 6 days ago by
Istvan Albert
99k • written 9 days ago by
Bjorn
• 0
2
votes
4
replies
336
views
How Can I move the scattered dots more closer into the center of box ?
R
Boxplots
geom_jitter
4 days ago by
ohtang7
▴ 40
0
votes
3
replies
336
views
RNA seq
Differential-gene-expression
updated 1 day ago by
jv
★ 1.3k • written 3 days ago by
Byonkesh
• 0
1
vote
3
replies
336
views
Bam files generated with STAR cause a segmentation fault core dump error when used with another tool
RNA-Seq
mapping
updated 3 days ago by
GenoMax
136k • written 3 days ago by
bkffadia
• 0
1
vote
3
replies
335
views
Indexing by bowtie2 but aligning with STAR
STAR
mm10
Bowtie2
updated 2 days ago by
Ram
41k • written 11 days ago by
Anojan
• 0
6
votes
5
replies
334
views
Calculate GC content for entire chromosome
bam
GC
assembly
bwa-mem
updated 18 hours ago by
colindaven
5.8k • written 1 day ago by
skilleta0527
• 0
4
votes
4
replies
329
views
Is there a need to batch correct FPKM or TPM values for within sample comparison
Batch
correction
6 days ago by
CTLong
▴ 30
2
votes
3
replies
317
views
Finding human .vcf files online to download
human
findvcf
vcf
updated 2 days ago by
cmdcolin
★ 3.4k • written 5 days ago by
eren.idil
▴ 10
2
votes
2
replies
316
views
Best method for batch correction of three datasets
RNA-seq
updated 1 day ago by
ATpoint
78k • written 1 day ago by
CTLong
▴ 30
0
votes
2
replies
313
views
Cellcyclescoring did not work in Seurat V5
singlecell
updated 6 days ago by
fracarb8
★ 1.3k • written 7 days ago by
synat.keam
▴ 80
0
votes
3
replies
307
views
Can FPKM be used to create bar graphs for DEGs?
RNA-seq
updated 2 days ago by
Ram
41k • written 20 days ago by
junli1988
• 0
3
votes
2
replies
305
views
Should I scale all genes in single cell Seurat?
singlecell
5 days ago by
synat.keam
▴ 80
2
votes
4
replies
304
views
An issue with gtf file (ballgownrsem)
Ballgown
RNA-seq
GTF
RSEM
18 hours ago by
cucindarko51
• 0
1
vote
7
replies
301
views
How to download multiple genome files using command line (MacOS) using datasets
ncbi-datasets
Bacteria
Genome
updated 1 day ago by
Ram
41k • written 2 days ago by
scholaraspect2008
• 0
0
votes
3
replies
300
views
String App in Cytoscape
App
String
cytoscape
3 days ago by
hellokwmin
• 0
1
vote
3
replies
299
views
best BLAST/alignment hits - which criteria is more important?
blast
1 day ago by
min
• 0
0
votes
5
replies
296
views
calculate nucleotide diversity from whole-genome-sequence data for individual genes
genomics
nucleotide_diversity
vcf
updated 2 days ago by
manaswwm
▴ 490 • written 7 days ago by
J
• 0
3
votes
2
replies
292
views
Mapping quality in `vg giraffe`
vg
6 days ago by
Wenhai
• 0
0
votes
2
replies
289
views
Guidance on Crucial Parameters for Bioinformatic Analysis of Metabolomic Data
metabolomic
bioinformatic
updated 6 days ago by
Kevin Blighe
86k • written 6 days ago by
Giovana
• 0
0
votes
6
replies
289
views
Resolving over clustered NGS with Q-scores
NGS
phred
Q-score
overclustering
11 hours ago by
sam.himes92
• 0
0
votes
1
reply
288
views
Gene Ontology enrichment with BINGO and ClueGO
BINGO
Cytoscape
ClueGO
Gene
Ontology
updated 6 days ago by
Scooter
▴ 180 • written 20 days ago by
David R
▴ 20
1
vote
1
reply
287
views
Forum:
want to work in open source project/project in bioinformatics
project
updated 2 days ago by
Ram
41k • written 11 days ago by
Afeef
• 0
4
votes
0
replies
282
views
Tool:
NOEL: An extremely fast Non-Overlapping Exon Length calculator written in Rust
gene-annotation
exon-length
4 days ago by
alejandrogzi
▴ 110
1
vote
3
replies
277
views
An incomprehensible error with R package gggenes
R
gggenes
visualize
updated 5 days ago by
ATpoint
78k • written 6 days ago by
alice005201314
• 0
0
votes
3
replies
273
views
using RSEM with non Trinity assembly
SPAdes
Trinity
RSEM
updated 2 days ago by
Dunois
★ 2.4k • written 9 days ago by
jen
• 0
4
votes
2
replies
272
views
KEGG DATABASE
DATABASE
KEGG
updated 1 day ago by
jv
★ 1.3k • written 4 days ago by
Sijjil
• 0
0
votes
2
replies
269
views
How should I run ssgsea analysis ?
functional
TPM
enrichment
gsea
ssgsea
updated 2 days ago by
Ram
41k • written 8 days ago by
DareDevil
★ 3.3k
0
votes
4
replies
267
views
Filling gaps in BAM file
bamtools
samtools
gene
bam
updated 1 day ago by
seidel
11k • written 2 days ago by
hemr3
• 0
2
votes
3
replies
266
views
Error in blast+
PowerShell
blast
Windows
updated 2 days ago by
Ram
41k • written 3 days ago by
Alexis
• 0
2
votes
4
replies
262
views
error when installing gistic2 using conda
conda
gistic2
updated 1 day ago by
Ram
41k • written 2 days ago by
luckyday1661
• 0
5
votes
2
replies
263
views
Fastest way to convert BED to GTF/GFF with gene_ids?
gff
convert
bed
gtf
updated 4 days ago by
cmdcolin
★ 3.4k • written 4 days ago by
alejandrogzi
▴ 110
0
votes
3
replies
261
views
In what case there are RR reads but no LL reads when detect inversions using IGV?
IGV
inversion
updated 6 days ago by
Istvan Albert
99k • written 6 days ago by
Jingfang
• 0
2
votes
4
replies
257
views
Can you help me to download list of miRNA from a SRA under a bioproject ?
bioproject
mirna
updated 6 days ago by
Nitin Narwade
★ 1.5k • written 7 days ago by
abourrieau
• 0
0
votes
2
replies
256
views
Low coverage whole genome sequencing reveal excess heterozygosity for multiple SNPs. How to filter?
lcWGS
WGS
heterozygosity
5 days ago by
beausoleilmo
▴ 560
0
votes
2
replies
255
views
How to identify CG, CHG, or CHH from MeDIP data
MethylationStudy
CHG
CHH
CG
MeDIP
2 days ago by
Tm
★ 1.1k
208 results • Page
2 of 5
Recent Votes
Answer: Calculate GC content for entire chromosome
STAR ALIGNMENT
STAR ALIGNMENT
Best method for batch correction of three datasets
CITEseq tutorial
C: PCA vs ICA prior to t-SNE or UMAP
C: PCA vs ICA prior to t-SNE or UMAP
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Comment: absolute running error: Error in if (!is.na(res)) { : the condition has length >
by
qiuting
• 0
this is the error.log: Detected error in sample P01_1 Error message: the condition has length > 1 Where: if !is.na(res) { mode.tab[(i…
Comment: STAR ALIGNMENT
by
Hải Đăng
• 0
Sis, where are you currently working? How can I get in touch with you?
Answer: RNA-Seq Data Analysis Workshop (March 11 - 14, 2024 in Berlin, Germany)
by
David Langenberger
10k
We already have an incredible number of registrations for this time. If you still want to be there, then you should register soon!
Comment: scRNA data analysis , how to compare pattern in multiple samples
by
ATpoint
78k
Can you please ask a precise question rather than posting a wall of code? What is the main problem you have, what did you try? Did you foll…
Comment: vcftools
by
sevda
• 0
Hello, Thank you for your attention. It ran successfully and I got no errors or warnings but no new files were created.
Comment: Resolving over clustered NGS with Q-scores
by
sam.himes92
• 0
The system that we used was [Aviti][1]. Our sequencing core was the one who quantified the loading/library concentration. Sorry I don't hav…
Comment: Where can I get a list of SNPs mapping overlapping genes in humans?
by
Alex Reynolds
35k
I modified the answer with an approach for that use case.
Comment: Where can I get a list of SNPs mapping overlapping genes in humans?
by
Ram
41k
OP is looking for overlapping genes - genes with presumably different gene IDs that share some loci. I think the `genes.bed` creation logic…
Comment: Where can I get a list of SNPs mapping overlapping genes in humans?
by
Alex Reynolds
35k
Note: The file `snps.bed` will be very large. You'll need sufficient disk space for this step.
Answer: Where can I get a list of SNPs mapping overlapping genes in humans?
by
Alex Reynolds
35k
Given files `genes.bed` and `snps.bed`, you could do something like: ``` $ bedmap --echo --echo-map-id --delim '\t' genes.bed snps.bed > a…
Answer: How to add exon annotations to genome annotation
by
alex.zaccaron
▴ 380
You can use [AGAT][1] to process your annotation file to add the missing exons. I believe `agat_convert_sp_gxf2gxf.pl -g no_exons.gff -o wi…
Comment: Annotation Visualization IGV
by
Pierre Lindenbaum
158k
and you got answer that should be validate (green tick), or people asked you questions but got no answers.
Comment: Annotation Visualization IGV
by
awhale01
• 0
Correct I have asked those questions before.
Comment: Annotation Visualization IGV
by
Pierre Lindenbaum
158k
you asked questions before: review, comment and/or validate them please: https://www.biostars.org/p/9530777/ ; https://www.biostars.org/p/9…
Comment: Ambient RNA removal method that generates whole (integer) counts
by
EK
• 0
Thanks, Jared. Sounds like rounding counts is the simplest approach for now. :)
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