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247 results • Page
3 of 5
Sort: Views
Rank
Views
Votes
Replies
3
votes
3
replies
461
views
Free/open source 23andme-like analysis
SNPs
genomics
NGS
VCF
updated 4 days ago by
swbarnes2
14k • written 5 days ago by
joe
▴ 510
4
votes
11
replies
453
views
Redirection of Duplicate PMIDs
pubmed
pmid
updated 17 hours ago by
LauferVA
4.2k • written 1 day ago by
dominickd
• 0
7
votes
6
replies
441
views
Filtering sam or bam file with maximum matching region
crisper-edited
alignment
match
3 days ago by
analyst
▴ 30
2
votes
5
replies
422
views
Marking duplicates using UMIs
Deduplication
UMI
updated 2 days ago by
i.sudbery
19k • written 3 days ago by
Lipika
• 0
0
votes
6
replies
421
views
Use of Kraken output for functional analysis
metagenomics
kraken
shotgun
updated 9 hours ago by
gv
• 0 • written 23 days ago by
Srinka
▴ 20
3
votes
4
replies
418
views
biomaRt error for different R version
biomaRt
R
updated 6 days ago by
GenoMax
141k • written 7 days ago by
manaswwm
▴ 490
0
votes
2
replies
416
views
QC exclusion of A/T or G/C alleles to avoid strand issues
quality-control
SNV
QC
SNP
5 days ago by
_quantum_girl_
▴ 10
1
vote
1
reply
409
views
Plink merge errors.
cygwin64
bed
PLINK
5 days ago by
Dinmukhamed
• 0
1
vote
1
reply
408
views
Difference when using difference number of pathways score to compare between 3 groups
GSVA
Limma
updated 3 days ago by
Gordon Smyth
★ 7.0k • written 6 days ago by
Chris
▴ 260
0
votes
2
replies
407
views
miRNAs quantification using mirdeep2 tool
differential-expression
mirdeep2
miRNA
5 days ago by
Ashok
• 0
2
votes
3
replies
405
views
Sheep reference genome remapping coordinates
reference
remap
sheep
coordinates
genome
6 days ago by
valentinatsar
• 0
1
vote
4
replies
401
views
GTF file for Rhinolophus sinicus
Rhinolophus-sinicus
GTF
updated 14 hours ago by
atowns21
• 0 • written 5 months ago by
ara
• 0
0
votes
1
reply
398
views
How to add rowLinks, rowTree in SummarizedExperiment manually.
phylogenetic-tree
microbiome
updated 6 days ago by
Ram
43k • written 19 months ago by
Muhammad
• 0
2
votes
2
replies
390
views
Using vg gamsort with naive sorting algorithm
gamsort
vg
updated 1 day ago by
Pierre Lindenbaum
161k • written 8 months ago by
AshleeThomson
▴ 80
1
vote
1
reply
387
views
Load a full GFF3 into annotation track using arrow (Apollo)
arrow
python-apollo
Apollo
5 days ago by
renan.igor
• 0
3
votes
9
replies
375
views
Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
FASTQ
updated 5 hours ago by
Istvan Albert
100k • written 1 day ago by
Κοσμάς
• 0
2
votes
2
replies
369
views
differential gene expression analysis when not all samples have an untreated counterpart
differential-expression
edgeR
6 days ago by
nhaus
▴ 300
0
votes
2
replies
368
views
error in coding potential calculator CPC2
cpc
CPC2
coding-potential
5 days ago by
Ashok
• 0
0
votes
2
replies
365
views
How can I solve this error?
metal
6 days ago by
22211020193
• 0
1
vote
1
reply
365
views
Problem with Mirdeep2 and Randfold output
mirdeep2
Randfold
6 days ago by
otieno43
▴ 30
0
votes
3
replies
352
views
How to identify gaps in a genome?
awk
seqkit
assembly
fasta
genome
updated 1 day ago by
colindaven
6.4k • written 9 days ago by
rj.rezwan
• 0
0
votes
10
replies
339
views
How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq libraries?
STARSolo
scRNA-seq
STAR
snRNA-seq
MGI
15 hours ago by
atowns21
• 0
0
votes
2
replies
339
views
How to add motifs in cellranger-arc reference?
gtf
scRNAseq
fasta
cellranger
updated 6 days ago by
Ehsan
• 0 • written 9 weeks ago by
bioinfo
▴ 150
4
votes
2
replies
310
views
Assume "yes" as the answer to any prompts with bioconda
docker
container
bioconda
updated 6 days ago by
Juke34
8.5k • written 6 days ago by
njornet
▴ 20
0
votes
2
replies
306
views
GSEA analysis in R
GSEA
R
Arabidopsis
6 days ago by
Sudip
• 0
2
votes
3
replies
303
views
How to establish haplotype-specific gene expression levels
RNA-seq
Haplotype
updated 2 days ago by
dsull
★ 5.8k • written 15 days ago by
javanokendo
▴ 60
1
vote
1
reply
293
views
GTF file from Stringtie to R Studio for either Ballgown or DESeq2
Stringtie
Cufflinks
Ballgown
DESeq2
updated 4 days ago by
Ram
43k • written 4 days ago by
Nina
• 0
1
vote
5
replies
286
views
DYH17 Structure Prediction
BLAST
Protein-Structure-Prediction
1 day ago by
anasjamshed
▴ 120
2
votes
1
reply
281
views
Running blastp with BLAST+ 2.15.0 against custom database; need to identify hits
shotgun
metagenomics
blastp
taxonomy
updated 4 days ago by
GenoMax
141k • written 4 days ago by
rebecca.calvo
• 0
0
votes
1
reply
278
views
Could you please assist in identifying this cluster?
single-cell
updated 5 days ago by
Ram
43k • written 5 days ago by
Kazo
• 0
0
votes
6
replies
278
views
How to slice a gvcf file with tabix?
Tabix
bed
gvcf
22 hours ago by
Sd
• 0
0
votes
0
replies
272
views
Choice of relatedness cutoff for GWAS of a large cohort (>100K samples)
relatedness
IBD
GWAS
updated 1 day ago by
Ram
43k • written 14 months ago by
Oak
▴ 10
0
votes
8
replies
270
views
Error in cnetplot enrichplot package
R
updated 1 day ago by
Ram
43k • written 1 day ago by
Farhad
• 0
0
votes
1
reply
264
views
assembly using CCS, CLR, CCS_CLR sequences together?
assembly
updated 6 days ago by
GenoMax
141k • written 6 days ago by
ycts
• 0
0
votes
1
reply
262
views
10x VDJ (TCR/BCR) BAM to fastq
TCR
VDJ
BCR
10x
updated 6 days ago by
GenoMax
141k • written 6 days ago by
predeus
★ 1.9k
0
votes
1
reply
262
views
DEseq2
RNA-seq
DESeq2
updated 6 days ago by
Ram
43k • written 6 days ago by
rrehimi
• 0
2
votes
4
replies
261
views
What is the real meaning of relative enrichment/peak height of ChIP-seq tracks?
ChIP-seq
16 hours ago by
HyperEvo
• 0
0
votes
1
reply
258
views
Downloading the raw microarray data from GEO
.gpr
microarray
.CEL
updated 5 days ago by
GenoMax
141k • written 5 days ago by
bioyas
▴ 10
3
votes
2
replies
256
views
p-value and Boneferroni adjusted p-value the same in R
Bonferroni
R
Statistics
p-value
Fisher
2 days ago by
kristiewyleung
• 0
2
votes
1
reply
255
views
Pediococcus acidilactici sequence analysis
BLAST
Gene-Ontology
updated 6 days ago by
Ram
43k • written 6 days ago by
anasjamshed
▴ 120
0
votes
5
replies
252
views
Should I remove unlocalized scaffols in reference genome before alignment?
qualimap
alignment
minimap2
GRCh38
assembly
7 hours ago by
njornet
▴ 20
1
vote
1
reply
250
views
Removing Illumina microarray probes with no expression HumanHT-12v4
microarray
HumanHT-12v4
Illumina
updated 4 days ago by
Gordon Smyth
★ 7.0k • written 4 days ago by
Bioinformatician
• 0
0
votes
2
replies
249
views
bam merging for archaic samples
samtools
bam
updated 3 days ago by
Ram
43k • written 4 days ago by
Matteo Ungaro
▴ 100
1
vote
5
replies
236
views
Downsampling fastq file
downsample
fastq
updated 2 days ago by
Ram
43k • written 2 days ago by
marco.barr
▴ 80
2
votes
4
replies
234
views
interpretartion of a vcf file
calling
genotype
ONT
variant
diploid
10 hours ago by
samuelkalandarov2002
▴ 10
3
votes
5
replies
234
views
Per base sequence content error in RNAseq analysis
RNA-seq
analysis
updated 2 days ago by
GenoMax
141k • written 2 days ago by
ahmad.sajad4541
• 0
0
votes
4
replies
232
views
Should I use unpaired reads from trimmomatic
RNA-seq
QC
Trimmomatic
updated 20 hours ago by
swbarnes2
14k • written 1 day ago by
dxj294
• 0
4
votes
5
replies
226
views
To get p-values for the TPM
P-value
TPM
RSEM
updated 1 hour ago by
swbarnes2
14k • written 12 hours ago by
VITALA
• 0
1
vote
2
replies
225
views
permutation test in edgeR
rna-seq
edgeR
updated 2 days ago by
Gordon Smyth
★ 7.0k • written 16 days ago by
Netanel
• 0
1
vote
3
replies
224
views
some error in building kraken2 database
metagenome
kraken2
17 hours ago by
Art1ess
• 0
247 results • Page
3 of 5
Recent Votes
Comment: To get p-values for the TPM
A: Where To Download Pam50 Gene Set?
A: Where To Download Pam50 Gene Set?
Answer: Fail to install umap-learn in R
Answer: Fail to install umap-learn in R
Answer: Fail to install umap-learn in R
What are Tiles in Sequencing
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Recent Replies
Comment: Troubleshooting RNA-seq data with DNA contamination
by
GenoMax
141k
Please post information about your tool as a `tools` category post. Don't wait/ask other users to ask you.
Comment: Adding CB tag to bam file
by
Maria
• 0
Sorry for the delay in my answer, I got sidetracked. I am using ```samtools addreplacerg -r "@RG\tID:$cell" -o $cell.bam -h $LINE``` bu…
Answer: Troubleshooting RNA-seq data with DNA contamination
by
haibol2017
▴ 10
We just developed a R package to tackle this problem. Do you have stranded or unstranded RNA-seq data contaminated with gDNA? If you wou…
Comment: How to trim transcripts using information from NCBI contamination screen report
by
GenoMax
141k
`bbduk.sh` can use the transcripts as input. There is a `adapters.fa` included in the `resources` directory of the distribution. You can do…
Comment: PCA plot (Suggestions Needed)
by
swbarnes2
14k
Cross posted here (including the code used to make up the counts): https://support.bioconductor.org/p/9157911/#9157959
Comment: To get p-values for the TPM
by
swbarnes2
14k
RSEM returns a kind of p-value if you run it with the "prior-enhanced" option. Did you do that? Are you completely sure that's the value y…
Comment: How to trim transcripts using information from NCBI contamination screen report
by
Lada
▴ 30
i checked both of these programs (`bbduk` and `fastp`) and it seems they use reads as inputs (fastq formats)... how can I trim my transcrip…
Comment: AGeNT LocatIt
by
barslmn
★ 2.1k
He is already giving 32G, 320G for marking duplicates for exome is excessive. Is there a specific reason for using the tool?
Comment: How to convert plink files to Hapmap Format
by
bk11
★ 2.3k
This looks good. Please try with using `--allow-no-sex flag` plink --bfile your_data_phenoUP --assoc --allow-no-sex --out your_data_ph…
Comment: How to convert plink files to Hapmap Format
by
Sofia
• 0
SC899359_PC75420_A05 SC899359_PC75420_A05 0 0 0 2 SC899360_PC75415_D01 SC899360_PC75415_D01 0 0 0 2 SC899361_PC75425_H05 SC899361_PC75425_H…
Comment: How to convert plink files to Hapmap Format
by
bk11
★ 2.3k
Please show me a few lines of phenotype updated .fam file. You can do `head your_data_phenoUP.fam` and paste result here. Please do not us…
Comment: GEMMA GWAS how to specify factor or numerical for covariates
by
Sofia
• 0
Hi, I don't have a solution to your question but I followed this tutorial and was helpful to me in my analysis https://youtu.be/NusV6duyvXs…
Comment: Is it possible to get a list of representative genomes from a past RefSeq releas
by
GenoMax
141k
Catalog file lists the genomes included. There seem to be 71365 unique items in field 2. 2 Bacteria WP_000018326.1 4459…
Comment: How to convert plink files to Hapmap Format
by
Sofia
• 0
yes sure ! ![enter image description here][1] [1]: /media/images/f0f94f6c-4387-4188-a1aa-19152a89
Answer: AGeNT LocatIt
by
Greg
• 0
Give it more memory. ~10x more memory, or whatever you can give it, I had samples that weren't even close to being done after 3.5 da…
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