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1,000 results • Page 2 of 20
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24
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44
replies
12k
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How to remove contamination from the transcriptome assembly
RNA-Seq contamination assembly blast Hiseq reads
updated 7 months ago by 38k • written 7.4 years ago by ★ 1.8k
152
votes
44
replies
28k
views
29 follow
Which Bioinformatic Friendly Pipeline Building Framework?
scripting
updated 8 months ago by 38k • written 9.2 years ago by ★ 2.0k
57
votes
43
replies
14k
views
8 follow
Forum: Best RNA-Seq aligner: A comparison of mapping tools
NGS alignment genome RNA-Seq sequence
updated 12 days ago by 38k • written 4.2 years ago by 9.9k
185
votes
43
replies
78k
views
29 follow
Drawing Chromosome Ideograms With Data
chromosome visualization ideogram
updated 6 months ago by 38k • written 13.0 years ago by ★ 1.6k
59
votes
43
replies
18k
views
18 follow
Tutorial: Polish PacBio assembly with latest PacBio tools : an affordable solution for everyone
pbalign pacbio arrow smrt-limk
updated 7 days ago by 38k • written 5.5 years ago by ★ 1.4k
151
votes
42
replies
174k
views
22 follow
How Do I Draw A Heatmap In R With Both A Color Key And Multiple Color Side Bars?
heatmap r
updated 10 months ago by 38k • written 11.0 years ago by 20k
122
votes
42
replies
8.7k
views
21 follow
News: Help Make Biostar Better! Add Your Feedback, Opinions And Suggestions.
meta biostars
updated 5 weeks ago by 38k • written 10.3 years ago by 96k
8
votes
42
replies
3.6k
views
NaS (Nanopore Synthetic-long) help
Assembly preprocessing
updated 11 weeks ago by 38k • written 7.8 years ago by ▴ 290
78
votes
41
replies
28k
views
24 follow
Tutorial: Extract Total Non-Overlapping Exon Length Per Gene With Bioconductor
fpkm rna-seq bioconductor
updated 3 months ago by • 0 • written 9.4 years ago by ★ 7.7k
136
votes
41
replies
20k
views
20 follow
Forum: Are We Rude/Do We Expect Too Much From People Asking Questions On This Forum?
meta
updated 25 days ago by 38k • written 9.8 years ago by ★ 1.6k
16
votes
41
replies
7.7k
views
How to extract information from headers of fasta file
sequence
updated 9 months ago by 38k • written 8.0 years ago by ▴ 70
23
votes
41
replies
13k
views
10 follow
WGCNA modules and categorical traits relationship
WGCNA
updated 4 months ago by • 0 • written 5.2 years ago by ▴ 110
167
votes
41
replies
85k
views
27 follow
Multiline Fasta To Single Line Fasta
sequence fasta
updated 7 weeks ago by • 0 • written 11.8 years ago by ▴ 240
18
votes
41
replies
10k
views
6 follow
paired end illumina reads
Assembly paired end
updated 8 months ago by 38k • written 7.4 years ago by ▴ 290
202
votes
41
replies
23k
views
20 follow
Forum: Genomics is not Special. Computational Biologists are reinventing the wheel for big data biology analysis
genomics cram gatk galaxy
updated 5 weeks ago by 38k • written 8.3 years ago by ★ 5.2k
17
votes
40
replies
16k
views
16 follow
Tutorial: Easy way to run easily orthoMCL (Copy & paste)
all-v-all orthomcl
updated 7 days ago by 38k • written 6.7 years ago by ▴ 160
6
votes
40
replies
9.0k
views
Fastx_Collapser Has No Fastq Output
fastx RNA-seq
updated 21 days ago by 38k • written 9.5 years ago by ▴ 240
151
votes
39
replies
22k
views
20 follow
Forum: A Farewell To Bioinformatics
bioinformatics
updated 26 days ago by 38k • written 10.2 years ago by 3.0k
25
votes
39
replies
4.4k
views
8 follow
Forum: Bioinformatics Study Group
study-group
updated 22 days ago by 38k • written 9.7 years ago by ▴ 440
226
votes
39
replies
281k
views
33 follow
Gene Id Conversion Tool
mapping conversion
updated 7 months ago by 38k • written 13.4 years ago by ▴ 620
276
votes
39
replies
164k
views
29 follow
Tutorial: How to download raw sequence data from GEO/SRA
fastq SRA download GEO bam
updated 10 months ago by 38k • written 8.6 years ago by 20k
81
votes
39
replies
6.0k
views
11 follow
News: State Of Biostar - Future Directions (January 2013)
biostars
updated 5 weeks ago by 38k • written 10.2 years ago by 96k
39
votes
38
replies
7.0k
views
9 follow
DNA composition - all k-mers and their frequency in some sequencing data
sequencing
updated 7 months ago by 38k • written 7.4 years ago by 13k
90
votes
38
replies
128k
views
18 follow
Extract Reads From A Bam File That Fall Within A Given Region
bam
updated 6 months ago by ▴ 10 • written 10.7 years ago by ▴ 370
223
votes
38
replies
134k
views
29 follow
Tool: Converting Genome Coordinates From One Genome Version To Another (Ucsc Liftover, Ncbi Remap, Ensembl Api)
genome coordinates conversion liftover ensembl Tool
updated 3 months ago by ▴ 90 • written 10.1 years ago by 19k
16
votes
38
replies
15k
views
Differential gene Analysis by Limma
Limma Microarray R
updated 11 months ago by 38k • written 8.1 years ago by ▴ 920
159
votes
38
replies
15k
views
22 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
updated 26 days ago by 38k • written 10.0 years ago by 96k
47
votes
37
replies
7.3k
views
12 follow
Forum: The Organisation Of Really Contrived Acronyms (Orca)
acronyms
updated 22 days ago by 38k • written 9.6 years ago by 2.3k
110
votes
37
replies
6.9k
views
13 follow
Bioinformatics Career Survey 2011/2012
career
updated 5 weeks ago by 38k • written 11.5 years ago by 153k
49
votes
36
replies
7.2k
views
11 follow
Forum: New features and bug fixes for Biostar to work on during Google Summer of Code
meta biostars gsoc
updated 5 weeks ago by 38k • written 8.9 years ago by ▴ 110
59
votes
36
replies
34k
views
27 follow
Pca From Vcf Files
pca genome vcf
updated 7 months ago by ▴ 40 • written 10.9 years ago by ▴ 820
8
votes
36
replies
9.9k
views
18 follow
Convert Gene IDs of Tomato Sol Database
tomato gene
updated 3 months ago by 38k • written 7.7 years ago by ▴ 160
251
votes
36
replies
30k
views
27 follow
Forum: How Do You Manage Your Files & Directories For Your Projects ?
file
updated 6 months ago by 84k • written 12.9 years ago by 153k
9
votes
36
replies
2.6k
views
6 follow
GPL6883_HumanRef-8_V3_0_R0_11282963_A (illumina expression beadchip)
R
updated 4 months ago by ★ 1.8k • written 2.3 years ago by ▴ 30
99
votes
36
replies
27k
views
32 follow
Forum: List of cloud genomics companies
cloud-genomics
updated 8 weeks ago by 21k • written 9.4 years ago by ★ 3.6k
146
votes
35
replies
58k
views
33 follow
Tutorial: Tools For Metagenomic Data Analysis
metagenomics next-gen
updated 26 days ago by 38k • written 10.3 years ago by ★ 1.6k
115
votes
35
replies
36k
views
20 follow
Forum: Snakemake vs. Nextflow: strengths and weaknesses
snakemake nextflow Forum
updated 11 days ago by ▴ 30 • written 5.8 years ago by ▴ 760
22
votes
35
replies
4.6k
views
Removing identifier with "unavailable sequence" from FASTA file
sequence genome blast RNA-Seq
updated 11 months ago by 38k • written 8.2 years ago by ★ 1.8k
45
votes
35
replies
4.6k
views
12 follow
Forum: looking for a bioinformatics project to do during military rotation
military
updated 8 months ago by 38k • written 5.5 years ago by ▴ 60
25
votes
35
replies
29k
views
16 follow
Calculate The Frequency Of Nucleotides At Each Position In An Mpileup File
mpileup
updated 7 weeks ago by ▴ 10 • written 9.0 years ago by ★ 4.1k
4
votes
35
replies
7.8k
views
Is They Any Software Or Technique To Find The Number Of Reads Mapped To Each Contig After Aligment
mapping RNA-seq
updated 19 days ago by 38k • written 9.2 years ago by ▴ 50
65
votes
35
replies
18k
views
17 follow
Which Dna Compression Algorithms Are Actually Used?
dna
updated 6 months ago by 153k • written 11.8 years ago by ▴ 270
116
votes
35
replies
7.0k
views
18 follow
Forum: Is Biostar Killing The Bioinformatics Core?
core biostars
updated 5 weeks ago by 38k • written 10.0 years ago by 21k
83
votes
34
replies
15k
views
21 follow
Tutorial: Enrichment Analysis, Clustering and Scoring with pathfindR
active-subnetwork enrichment pathway R
updated 12 days ago by 38k • written 4.7 years ago by ★ 1.3k
54
votes
34
replies
3.6k
views
13 follow
Forum: Should We Release Database Dumps Of All Questions On Biostar?
meta biostars
updated 5 weeks ago by 38k • written 12.3 years ago by 96k
80
votes
34
replies
3.9k
views
11 follow
Forum: Authorship issues- how to resolve?
authorship
updated 10 weeks ago by 38k • written 7.8 years ago by ▴ 520
15
votes
34
replies
10k
views
Improving efficiency of awk for large files
awk
updated 4 months ago by 38k • written 7.7 years ago by ▴ 350
16
votes
34
replies
11k
views
6 follow
Why am I unable to load my data from a tab separated file into R?
microarray programming R
updated 11 months ago by 38k • written 8.1 years ago by ▴ 920
145
votes
34
replies
51k
views
17 follow
Tutorial: Working with MAF files (Mutation Annotation Format) from the TCGA (The Cancer Genome Atlas)
music cancer tcga mutation maf
updated 25 days ago by 38k • written 9.9 years ago by 5.9k
42
votes
33
replies
6.8k
views
8 follow
Tutorial: How to Use Biostars, Part-I: Questions, Answers, Comments and Replies
meta how-to documentation
7 days ago by Ram 38k
1,000 results • Page 2 of 20
Recent Votes
Answer: RNA-SEQ
Comment: RNA-SEQ
Comment: RNA-SEQ
Answer: RNA-SEQ
Gene Set Enrichment Analysis
Can I sort my bam files with Picard MergeSamFiles?
Answer: Problem generating a .vcf after upgrade of samtools and bcftools
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Recent Replies
Comment: Can someone help me with searching overlapping values between two files? by mxm189 • 0
I tried some stuff but it didnt work. I used ChatGPT, because I'm not good at this. I tried sorting of the two files first # Sort the inp…
Comment: Survival analysis with gene expression by krushnach80 ★ 1.1k
Hello @kevinblighe I would like to get your help in [this][1] [1]: https://www.biostars.org/p/9558148/
Comment: activity of unloaded/unassembled Tn5 by callumjcparr • 0
![enter image description here][1] ![enter image description here][2] [1]: /media/images/72273297-373f-4654-9ddb-19b8859c [2]: /med…
Answer: How to implement this two-stage one-to-many workflow using WDL? by Ruben • 0
> Unfortunately, as far as I can tell, WDL provides no support for iterating over the contents of a directory. (I find this shocking. I con…
Answer: activity of unloaded/unassembled Tn5 by callumjcparr • 0
So I hope someone is still interested in this. We have taken to preparing recombinant Tn5 now as it's much cheaper than commercial suppli…
Comment: RNA-SEQ by ali • 0
thanks for your helping
Comment: TSS of protein coding genes by ConvolutedGenome ▴ 10
I am quite confused with GENCODE GTF file, So, within the GENCODE GTF file, I noticed that each (protein-coding) gene has multiple "transc…
Comment: Recommendation for (Alternative) Splicing analysis packages for bulk RNA-seq da by iraun 5.8k
Hi! I personally use [SplAdder][1], in case you want to add it to your list :). [1]: https://github.com/ratschlab/spladder
Answer: Problem generating a .vcf after upgrade of samtools and bcftools by Andy • 0
Really the only needed, that help worked, thank's
Comment: Active enhancers for mm39 by seidel 11k
Just curious what you mean by "active" enhancers. Enhancers are active in a given context (e.g. cell type developmental state, etc.), and m…
Comment: Creating loop for read groups using Picard by ntsopoul ▴ 20
try absolute paths navigate to the directory with cd and use pwd to get the absolute directory.
Comment: how to create interacting Hi-C peaks by rheab1230 ▴ 140
Thank you. I will use this package since I am more familiar with R
Comment: how to create interacting Hi-C peaks by rheab1230 ▴ 140
thank you. i will go through it
Comment: Fastqc report analysis by npavliukovec • 0
Yeah, after trimming and fastqc I will have to generate MultiQC plots and after do mapping with reference genome. As I know, for eukaryotes…
Comment: My fastq files(paired end) have different read number by kimgeng • 0
Oh, I see, Thank you however, I want to make vcf files with gatk haplotypecaller and GenotypeGVCFs. But I got some problems I have about 2.…
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