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1,000 results • Page 4 of 20
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65
votes
24
replies
16k
views
14 follow
Mapping Snps To Pathways
snp genotyping pathway gene enrichment
updated 7 weeks ago by 41k • written 13.8 years ago by 158k
65
votes
26
replies
13k
views
11 follow
Tutorial: Generating consensus sequence from bam file
fasta consensus bam
updated 9 months ago by 41k • written 4.8 years ago by 16k
65
votes
27
replies
6.9k
views
14 follow
Forum: What is the reason for most software errors in Bioinformatics according to you?
software-error blast sequencing SNP R
updated 8 months ago by 41k • written 7.7 years ago by ▴ 110
65
votes
10
replies
3.2k
views
6 follow
Incentive For Learning Emboss
emboss
updated 5 months ago by 41k • written 12.7 years ago by 11k
65
votes
26
replies
19k
views
13 follow
Forum: Macosx For Bioinformatics?
linux
updated 9 months ago by 41k • written 10.0 years ago by 11k
65
votes
9
replies
48k
views
7 follow
Tutorial: Reference Assembly - Mapping Reads To A Reference Genome
reference samtools mapping bwa
updated 10 months ago by 41k • written 10.5 years ago by ★ 3.0k
64
votes
24
replies
2.7k
views
12 follow
Forum: How much I must feel useless?
machine-learning Programming statistics
updated 6 months ago by 41k • written 5.2 years ago by ▴ 80
64
votes
8
replies
8.1k
views
7 follow
Forum: Isaac Asimov Accurately Predicts Woes Of Bioinformatician
bioinformatician
updated 9 months ago by 41k • written 10.6 years ago by 20k
64
votes
26
replies
13k
views
7 follow
Tutorial: Parallel processing in R
biocparallel mclapply foreach R
updated 8 months ago by 41k • written 6.2 years ago by 86k
64
votes
9
replies
105k
views
10 follow
Blog: Gene expression units explained: RPM, RPKM, FPKM and TPM
gene-expression RNA-Seq
updated 10 months ago by 41k • written 6.2 years ago by ★ 2.2k
64
votes
29
replies
15k
views
15 follow
Forum: Fundamentals Of Designing Biological Databases?
database
updated 9 months ago by 41k • written 13.1 years ago by ▴ 130
63
votes
15
replies
13k
views
Tutorial: Which human reference genome should I use?
assembly genome alignment
updated 6 months ago by 41k • written 5.2 years ago by 16k
63
votes
27
replies
26k
views
18 follow
Data Too Big To Be Stored In Memory: Common Options
python
updated 9 months ago by 41k • written 11.1 years ago by ▴ 720
63
votes
11
replies
15k
views
9 follow
Forum: Web Based Interactive Resources To Learn Programming And/Or Bioinformatics
programming
updated 9 months ago by 41k • written 10.4 years ago by 19k
63
votes
9
replies
3.8k
views
Forum: Should The "Post Closing" Option Exist? Vote Now!
meta
updated 9 months ago by 41k • written 10.5 years ago by 99k
63
votes
28
replies
3.6k
views
11 follow
Forum: Bioinformatics analyses & errors: need for better post-publication tools?
next-gen RNA-Seq
updated 8 months ago by 41k • written 7.6 years ago by ★ 1.2k
62
votes
16
replies
33k
views
18 follow
Job: Bioinformatics Jobs You Can Do From Home?
python education
updated 9 months ago by 41k • written 13.2 years ago by ▴ 290
62
votes
17
replies
34k
views
7 follow
how to remove multiallelic from VCF
SNP
updated 10 months ago by 41k • written 8.6 years ago by ▴ 530
62
votes
29
replies
7.2k
views
14 follow
Best bioinfo one-liners?
linux
updated 11 months ago by 41k • written 8.6 years ago by 4.1k
62
votes
24
replies
41k
views
12 follow
How to extract unique mapped results from Bowtie2 bam results?
alignment
updated 11 months ago by 41k • written 9.5 years ago by ▴ 170
62
votes
14
replies
192k
views
9 follow
How To Deal With Duplicate Row Names Error In R
r
updated 7 months ago by 11k • written 10.8 years ago by ▴ 900
62
votes
95
replies
18k
views
8 follow
How to extract the list of genes from TCGA CNV data
TCGA CNV genes
updated 10 months ago by ▴ 10 • written 5.6 years ago by ▴ 260
61
votes
6
replies
9.3k
views
Tutorial: Minimum Standards For Bioinformatics Command Line Tools
command-line
updated 9 months ago by 41k • written 10.3 years ago by 9.7k
61
votes
16
replies
7.4k
views
14 follow
Forum: Rust or C++, what to learn after Go for high-performance bioinformatics tools?
library
updated 11 months ago by ★ 1.3k • written 3.1 years ago by 8.3k
61
votes
14
replies
11k
views
11 follow
Recommend Easy To Use Microarray Clustering Software
microarray clustering
updated 7 weeks ago by 41k • written 14.2 years ago by 99k
61
votes
51
replies
21k
views
9 follow
Tool: Efficiently process (view, analize, clip ends, convert, demultiplex, dereplicate) SFF/FastQ files
sff sequence fastq next-gen
updated 5 months ago by 41k • written 9.6 years ago by ▴ 790
61
votes
23
replies
54k
views
15 follow
How Can I Know The Length Of Mapped Reads From Bam File?
bam mapping
updated 18 days ago by ★ 1.2k • written 10.8 years ago by ★ 1.3k
61
votes
70
replies
13k
views
21 follow
Forum: Biostar 2.0 released
meta biostars
updated 9 months ago by 41k • written 9.7 years ago by 99k
61
votes
14
replies
19k
views
13 follow
Why is Hadoop not used a lot in bio-informatics?
hadoop
updated 9 months ago by 41k • written 9.2 years ago by ★ 5.2k
60
votes
14
replies
12k
views
16 follow
Forum: Interesting bioinformatics blogs (2017 edition)
blogs
updated 9 months ago by 41k • written 6.7 years ago by 47k
60
votes
28
replies
7.9k
views
27 follow
Forum: Jim Robinson of the Integrative Genomics Viewer (IGV) uses this
uses-this
updated 9 months ago by 41k • written 9.2 years ago by 99k
60
votes
17
replies
33k
views
16 follow
Scientific Names In Blast Output And Databases
blast blast+
updated 11 months ago by 41k • written 10.4 years ago by ★ 2.1k
60
votes
52
replies
42k
views
23 follow
Tutorial: Creating Interactive Volcano Plots with R and Plot.ly
plotly R volcano-plot
updated 8 months ago by 41k • written 7.2 years ago by ★ 3.4k
60
votes
2
replies
7.2k
views
Tutorial: Table Of Contents To All Review Paper Compilations On Biostar
papers
updated 9 months ago by 41k • written 11.3 years ago by 99k
60
votes
21
replies
3.3k
views
14 follow
Reproduce the article "The complete sequence of a human genome."
genome assembly
updated 3 months ago by 53k • written 3 months ago by ▴ 60
60
votes
18
replies
3.7k
views
10 follow
Are Super User Style Questions Appropriate In Biostar?
meta biostars
updated 9 months ago by 41k • written 13.4 years ago by ▴ 460
60
votes
3
replies
32k
views
Paired-end reads - what is what?
RNA-Seq
updated 11 months ago by 41k • written 8.5 years ago by ▴ 220
60
votes
25
replies
21k
views
17 follow
Which Operating System Do You Prefer For Bioinformatics?
general subjective
updated 7 weeks ago by 41k • written 13.9 years ago by 28k
59
votes
25
replies
4.3k
views
8 follow
Forum: Am I crazy, or are most published RNA-seq studies vastly underpowered?
sample-size statistical-power RNA-Seq
updated 6 months ago by 41k • written 3.3 years ago by ▴ 220
59
votes
31
replies
4.5k
views
12 follow
News: Example of how bioinformaticians can publish in Scientific Reports (by nature publishing group) using publicly available NGS data
publication NGS
updated 8 months ago by 41k • written 6.9 years ago by 10k
59
votes
43
replies
20k
views
18 follow
Tutorial: Polish PacBio assembly with latest PacBio tools : an affordable solution for everyone
pbalign pacbio arrow smrt-limk
updated 8 months ago by 41k • written 6.2 years ago by ★ 1.4k
58
votes
30
replies
11k
views
13 follow
Content Management Systems For Bioinformatics Websites
web
updated 25 days ago by 41k • written 13.7 years ago by 18k
58
votes
25
replies
39k
views
15 follow
Tutorial: Protocol To Downlad TCGA Data From GDC
GDC methylation TCGA
updated 8 months ago by 41k • written 7.4 years ago by ★ 9.3k
57
votes
15
replies
4.6k
views
9 follow
Forum: Entice Biologists To Learn R
r
updated 9 months ago by 41k • written 12.2 years ago by 5.3k
57
votes
15
replies
5.8k
views
9 follow
Forum: Good practices while dealing with very large datasets
Big-Data Programming
updated 7 months ago by 41k • written 6.6 years ago by ★ 1.1k
57
votes
19
replies
27k
views
6 follow
Forum: Attention: Bowtie2 And Multiple Hits
next-gen bowtie mapping
updated 9 months ago by 41k • written 11.1 years ago by 10k
57
votes
22
replies
25k
views
7 follow
Batch correction in DESeq2
DESeq2 Batch Effect Combat
updated 9 months ago by 86k • written 4.2 years ago by ▴ 510
57
votes
9
replies
5.5k
views
14 follow
Forum: Handbooks for Bioinformatics Ninjas
gallery
updated 8 months ago by 41k • written 7.6 years ago by ★ 9.3k
57
votes
43
replies
15k
views
8 follow
Forum: Best RNA-Seq aligner: A comparison of mapping tools
NGS alignment genome RNA-Seq sequence
updated 9 months ago by 41k • written 4.9 years ago by 10k
56
votes
23
replies
4.6k
views
10 follow
Tool: ASCIIGenome: Text Only Genome Viewer!
terminal genome-browser java ASCIIGenome
updated 5 months ago by 41k • written 7.4 years ago by 14k
1,000 results • Page 4 of 20
Recent Votes
Comment: GATK GetPileupSummaries Java heap space
Answer: How to subtract variants from one VCF file to another?
What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
Answer: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 202
Answer: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 202
Answer: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 202
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Recent Replies
Comment: which statistical test and which package for comapre datasets? by Jeremy ▴ 860
I think you will need at least three replicates to be able to do statistics.
Comment: GetPileupSummaries intervals-list with Targeted Sequencing? by Pierre Lindenbaum 158k
> Asking for bed file is not a suitable option in the current scenario. ???????
Comment: GetPileupSummaries intervals-list with Targeted Sequencing? by Manuel Sokolov Ravasqueira ▴ 100
they only provided me the sorted.bam files aligned with hg19. I converted bam into fastq and started variant calling from there. Asking for…
Comment: GetPileupSummaries intervals-list with Targeted Sequencing? by Pierre Lindenbaum 158k
> kit is proprietary I can't imagine you get a targeted sequencing without having a BED file....
Comment: GetPileupSummaries intervals-list with Targeted Sequencing? by Manuel Sokolov Ravasqueira ▴ 100
thanks for the suggestion. how can i obtain the bedfile of my targets (kit is proprietary - can I obtain bed from fasta? if yes how?)
Comment: Is interleaved Fastq files the same as interlaced fastq by rpolicastro 12k
This may indicate that your R1 and R2 reads were processed in a way that didn't maintain the 1:1 order of matching forward and reverse read…
Comment: Is interleaved Fastq files the same as interlaced fastq by RCMC • 0
Sorry I accidentally hit enter BWA-MEM2 has occurring some error and states that paired reads have different names so I am trying to com…
Comment: GetPileupSummaries intervals-list with Targeted Sequencing? by Pierre Lindenbaum 158k
how about using the bed file of your targets....
Answer: LCA from BLAST output by Dunois ★ 2.4k
You should probably be using `MMseqs2`'s `taxonomy` module for this: [https://github.com/soedinglab/MMseqs2#taxonomy][1] . [1]: https:/…
Comment: autodock vina on mac by iamsmor • 0
thank you but ı am already follow this tutorial steps but at mgltools steps at the page but ı dont undertsand there isnot no enough explana…
Comment: How to create interval list from reference fasta or dict file? by Manuel Sokolov Ravasqueira ▴ 100
Hello @MatthewP , were you able to that for a fasta file for example? If you have a fasta file containing a targeted region, how to you ge…
Comment: WGCNA Dendrogram Branch missing from modules by andres.firrincieli 3.5k
I am not familiar with hdWGCNA but perhaps some modules are excluded because their genes are not expressed in a certain proportion of cells.
Comment: LCA from BLAST output by Michael 53k
If you know the lineage information is complete you could compare which columns are identical, however, there may be unranked levels in bet…
Comment: Is GeSeq currently unavailable? by GenoMax 136k
Email the developers listed on contact page: https://chlorobox.mpimp-golm.mpg.de/contact.html
Comment: Alternative to Gentrome? by GenoMax 136k
Curious as to what is `gentrome`? Genome decoy + transcriptome?
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