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373 results • Page
2 of 8
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0
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0
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74
views
News:
Online course: DNA methylation
Pacbio
Nanopore
DnaMethylation
Evolution
1 day ago by
carlopecoraro2
★ 2.5k
0
votes
0
replies
99
views
Job:
Ensembl Plants Senior Bioinformatician
ebi
embl
genomics
ensembl
1 day ago by
Ben_Ensembl
★ 2.4k
1
vote
2
replies
154
views
What is the real meaning of relative enrichment/peak height of ChIP-seq tracks?
ChIP-seq
10 hours ago by
HyperEvo
• 0
0
votes
1
reply
128
views
RNA-seq bacteria contamination
RNA-seq
updated 1 day ago by
GenoMax
141k • written 1 day ago by
sh
• 0
0
votes
2
replies
165
views
Result of running of p3_in.pl
primer3
updated 1 day ago by
GenoMax
141k • written 1 day ago by
nasri
• 0
0
votes
1
reply
115
views
FAM FILE
file
Fam
1 day ago by
Eleonora
• 0
0
votes
0
replies
63
views
Imputation advice
imputation
1 day ago by
kl
▴ 10
2
votes
5
replies
219
views
Per base sequence content error in RNAseq analysis
RNA-seq
analysis
updated 1 day ago by
GenoMax
141k • written 1 day ago by
ahmad.sajad4541
• 0
0
votes
0
replies
79
views
Trimming best practices for SNP calling
trimming
SNP
trimmomatic
1 day ago by
Enrique
• 0
3
votes
5
replies
523
views
SLURM submission problem: Indexing the Reference Genome is not done in STAR
align
RNA-Seq
linux
STAR
1 day ago by
n_navy
• 0
0
votes
0
replies
66
views
Correct way of reducing predictive model complexity
lasso
glmnet
predictvemodeling
regression
1 day ago by
sativus
▴ 20
6
votes
3
replies
151
views
Interpretting IGV output
IGV
updated 1 day ago by
Carlo Yague
8.7k • written 1 day ago by
analyst
▴ 20
0
votes
1
reply
159
views
homology modelling using swiss model
homology-modeling
swiss-model
updated 1 day ago by
colindaven
6.4k • written 2 days ago by
Ayush
• 0
0
votes
0
replies
68
views
Difficulties getting **the adjacency file** necessary for **aracne2regulon** function.
VIPER
MARINA
ARACNe
1 day ago by
Biostars2200
• 0
1
vote
3
replies
207
views
DYH17 Structure Prediction
BLAST
Protein-Structure-Prediction
11 hours ago by
anasjamshed
▴ 120
0
votes
0
replies
103
views
CIRIquant: ValueError: file has no sequences defined (mode='rb') - is it SAM/BAM format? Consider opening with check_sq=False
Ciriquant
updated 1 day ago by
Carlo Yague
8.7k • written 1 day ago by
Atul K.
• 0
3
votes
2
replies
240
views
p-value and Boneferroni adjusted p-value the same in R
Bonferroni
R
Statistics
p-value
Fisher
1 day ago by
kristiewyleung
• 0
1
vote
5
replies
394
views
Marking duplicates using UMIs
Deduplication
UMI
updated 1 day ago by
i.sudbery
19k • written 2 days ago by
Lipika
• 0
0
votes
0
replies
131
views
How to extract the mutations specific to cancer after variant annotation
variant-annotation
updated 1 day ago by
Ram
43k • written 2 days ago by
sainavyav22
• 0
0
votes
0
replies
132
views
Bacterial genome alignment with mauve
Bacteria
mauve
genome
2 days ago by
rthapa
▴ 90
0
votes
0
replies
124
views
Reparametrization of .str (param penalty > 100) File from CGenFF for Ligand Simulation in GROMACS
Simulation
CGenFF
Gromacs
VMD
2 days ago by
Mamatha Y S
• 0
0
votes
0
replies
122
views
News:
upcoming online statistical courses
Statistics
R
Data-Analysis
updated 1 day ago by
Ram
43k • written 2 days ago by
carlopecoraro2
★ 2.5k
0
votes
4
replies
492
views
how to find genes that are significantly expressed in brain cortex samples
rna-seq
gene-expression
updated 2 days ago by
ATpoint
81k • written 3 days ago by
rheab1230
▴ 140
0
votes
2
replies
240
views
bam merging for archaic samples
samtools
bam
updated 1 day ago by
Ram
43k • written 3 days ago by
Matteo Ungaro
▴ 100
7
votes
6
replies
418
views
Filtering sam or bam file with maximum matching region
crisper-edited
alignment
match
1 day ago by
analyst
▴ 20
1
vote
1
reply
244
views
Removing Illumina microarray probes with no expression HumanHT-12v4
microarray
HumanHT-12v4
Illumina
updated 3 days ago by
Gordon Smyth
★ 7.0k • written 3 days ago by
Bioinformatician
• 0
1
vote
0
replies
518
views
The majority of the sequences in Trinity ID do not start from start codons. Can the sequences still be used for primer design and validation?
Primers
TRINITY
3 days ago by
mathavanbioinfo
▴ 90
1
vote
1
reply
285
views
GTF file from Stringtie to R Studio for either Ballgown or DESeq2
Stringtie
Cufflinks
Ballgown
DESeq2
updated 3 days ago by
Ram
43k • written 3 days ago by
Nina
• 0
2
votes
5
replies
481
views
MEGA Analysis Issue on High-Performance Computer
phylogenetic-analysis
MEGA
updated 2 days ago by
Mensur Dlakic
★ 27k • written 3 days ago by
Giorgio
• 0
0
votes
1
reply
268
views
Running blastp with BLAST+ 2.15.0 against custom database; need to identify hits
shotgun
metagenomics
blastp
taxonomy
updated 3 days ago by
GenoMax
141k • written 3 days ago by
rebecca.calvo
• 0
0
votes
0
replies
201
views
Using samtools with GCS (google cloud storage) on a docker container seems to give "Protocol Not Supported" error
samtools
gcs
2 days ago by
abhishekghadge
• 0
2
votes
5
replies
657
views
what's the genomic location and name of gene responsible for orange and black patches in calico cats
genomes
updated 2 days ago by
Jesse
▴ 740 • written 3 days ago by
Ann
★ 2.4k
0
votes
0
replies
179
views
agilent microarray gpl data does not have gene symbol column
microarray
agilent
gpl
updated 3 days ago by
Ram
43k • written 3 days ago by
mja
• 0
0
votes
0
replies
181
views
scRNA Cluster frequency
CV
PCA
scRNA
Cluster-Frequency
3 days ago by
Nitin
• 0
1
vote
5
replies
528
views
Duplicate marking, read names, and the SRA
SRA
MarkDuplicates
updated 3 days ago by
GenoMax
141k • written 3 days ago by
Luka
• 0
0
votes
0
replies
181
views
absolute path for symbolic links in Snakefile
Snakemake
updated 3 days ago by
Ram
43k • written 3 days ago by
yifangt86
▴ 60
0
votes
0
replies
175
views
Chemical structure validation
structure
cap
validation
metabolite
3 days ago by
Rodolfo Adrián
• 0
3
votes
8
replies
1.2k
views
Cellranger count error: Input FASTQ file ended prematurely
scRNA-seq
Cellranger
STARsolo
10X
updated 3 days ago by
swbarnes2
14k • written 9 months ago by
bp22
▴ 80
0
votes
1
reply
250
views
Downloading the raw microarray data from GEO
.gpr
microarray
.CEL
updated 3 days ago by
GenoMax
141k • written 3 days ago by
bioyas
▴ 10
0
votes
0
replies
196
views
Copy number variation plot
Copy-number-variation
genomics
updated 3 days ago by
Ram
43k • written 3 days ago by
Emmi
• 0
1
vote
5
replies
678
views
RNA seq differential expression analysis
RNA-seq
normalization
updated 3 days ago by
BioinfGuru
★ 1.7k • written 5 days ago by
rrehimi
• 0
0
votes
0
replies
170
views
SNP calling with ANGSD and ngsLD. How many SNPs?
lcwgs
ngsTools
ANGSD
genomics
3 days ago by
DanielEB_fisk
▴ 20
0
votes
1
reply
271
views
Could you please assist in identifying this cluster?
single-cell
updated 3 days ago by
Ram
43k • written 4 days ago by
Kazo
• 0
4
votes
9
replies
2.6k
views
Tool:
CANEapp, a user-friendly application for automated next generation transcriptomic data analysis
RNA-Seq
written 8.2 years ago by
dvelmeshev
• 0
2
votes
7
replies
5.2k
views
6 follow
Memory usage of picard Samsort
Picard
SortSam
updated 4 days ago by
ATpoint
81k • written 7.1 years ago by
boymin2020
▴ 80
5
votes
13
replies
1.0k
views
Tutorial:
Installing/switching between versions of R/Rstudio/Bioconductor on personal machine (Linux | Ubuntu)
R
Ubuntu
Linux
Bioconductor
Rstudio
updated 3 days ago by
ATpoint
81k • written 4 days ago by
BioinfGuru
★ 1.7k
1
vote
1
reply
403
views
Plink merge errors.
cygwin64
bed
PLINK
4 days ago by
Dinmukhamed
• 0
0
votes
0
replies
189
views
Conversion to tree format
PLINK
bed
Figtree
SplitsTree
updated 3 days ago by
Ram
43k • written 4 days ago by
Dinmukhamed
• 0
0
votes
0
replies
188
views
miRDeep2: How to get the read counts
mirdeep2
4 days ago by
Atul K.
• 0
3
votes
4
replies
624
views
Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (without Blat)
mapping
22 hours ago by
rahu
• 0
373 results • Page
2 of 8
Recent Votes
The Biostar Herald for Monday, April 15, 2024
The Biostar Herald for Monday, April 15, 2024
Filter BAM to keep only alignments at an interval, ignoring reads spliced over
Answer: Gene set enrichment analysis differences between 2020 and 2024
Answer: Fast way to extract specific sequences from large fasta
Answer: Fast way to extract specific sequences from large fasta
Comment: Intersect multiple BED files
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Recent Awards •
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Scholar
to
anovak
▴ 110
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to
pfee418
▴ 10
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sarahmanderni
▴ 100
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Michael
54k
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Mensur Dlakic
★ 27k
Recent Replies
Comment: Using vg gamsort with naive sorting algorithm
by
Pierre Lindenbaum
161k
> Don't forget to follow up on your threads. If an answer was helpful, you should upvote it; if the answer resolved your question, you shou…
Comment: Redirection of Duplicate PMIDs
by
dominickd
• 0
The scope is all publications related to grants from an NIH award program. There are currently ~120k publications in total (about a few hun…
Answer: Using vg gamsort with naive sorting algorithm
by
anovak
▴ 110
The `-d` sorting mode does the entire sort in memory, so it will only work if you have enough memory to load your whole GAM file. To fin…
Comment: Redirection of Duplicate PMIDs
by
dominickd
• 0
I have a list of PMIDs for publications linked to a list of grants, exported from NIH RePORTER.
Comment: Plot DNA methylation level of DMR on the basis of certain genes
by
Pierre Lindenbaum
161k
> Don't forget to follow up on your threads. If an answer was helpful, you should upvote it; if the answer resolved your question, you shou…
Comment: Should I remove unlocalized scaffols in reference genome before alignment?
by
GenoMax
141k
Yes you can remove those if you are only interested in annotated genes.
Comment: Help for coding: Trinity for differential gene expression studies
by
mchour
• 0
[This could help][1] [1]: https://github.com/trinityrnaseq/RNASeq_Trinity_Tuxedo_Workshop/wiki/Trinity-De-novo-Transcriptome-Assembly-W…
Answer: How to compare the quality of assemblies
by
colindaven
6.4k
Are you sure you have looked into the HiFiasm output files (fasta and GFA) in detail and excluded non-primary contigs, hap1, hap2 and other…
Answer: How to identify gaps in a genome?
by
colindaven
6.4k
You can use seqtk to find gaps in a fasta https://github.com/lh3/seqtk seqtk gap Usage: seqtk gap [-l 50] <in.fa>
Comment: some error in building kraken2 database
by
GenoMax
141k
> Obviously, running kraken2 after the build is complete does not produce the correct results What do you get instead? Error/no output? Ha…
Comment: Failed kmer content
by
GenoMax
141k
Can you post a screenshot of what you are referring to? Why did you choose to "clean" your data? FastQC analysis needs to be done keeping…
Comment: Help for coding: Trinity for differential gene expression studies
by
rhossen
• 0
It should be great if I have any assistance in my build up command using trinity package...
Comment: Filter BAM to keep only alignments at an interval, ignoring reads spliced over
by
WouterDeCoster
47k
I want reads in a specific short interval, but if I do that with samtools view I also get reads that are spliced over that interval.
Answer: Help for coding: Trinity for differential gene expression studies
by
mchour
• 0
Hello, I would recommend following RSEM wiki for more information about the options, or even run RSEM directly, not with the script provi…
Answer: WGCNA preservation analysis
by
Michael
54k
This seems like a model case for why we need better sustainable bioinformatics data and services. I don't know the exact background behind …
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