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Comment: Correct way to remove Nextera adapters from ITS sequences
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Some of these programs have a minimum size threshold for a read to be retained. You could play around with that to keep all trimmed reads 5…
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Could it be that idents needs a character string instead of integers? Also, you have an extra comma in your call after c(0:16).
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`indents` is probably a factor so even though you've removed cells with those level values, the factor level still remains. Don't worry abo…
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I would still recommend using `minimap2` and aligning against reference. It should go pretty fast. You can either work with PAF (default) f…
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I've moved this to a comment as it does not directly answer OP's question.
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I'm very new myself and there's plenty of people who can answer your question better than I can, but here is a resource I found extremely h…
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If the number of contigs was so small I would manually check each alignment and edit the header of each contig,inserting the corresponding …
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No the library sizes across conditions should be relatively homogenous because it's from the same dataset. I clustered the samples into sub…
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It shouldn't, unless there's a misspecification in design. Is it the case that the library sizes are very different between the conditions …
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For a pure contig to Dmel chromosome BLAST should be OK.
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How many contigs do you have? If the contigs are ~2x number of Drosophila chromosomes (meaning you have long/large contigs) then using a lo…
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Thank you for your answer!
It would be wrong just to BLAST all the contigs from the draft assembly against each reference chromosome of D.…
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Several hundred to 10000 DEGs.
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Not sure if it is the easiest, but you can map your new assembly to i.e. Dmel genome using LAST:
* www: https://gitlab.com/mcfrith/last …
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