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757 results • Page
3 of 16
Sort: replies
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Views
Votes
Replies
0
votes
7
replies
792
views
Mugsy error -directory must be a directory
Mugsy
updated 10 days ago by
Ram
40k • written 2.8 years ago by
A_heath
▴ 140
5
votes
7
replies
508
views
Elusive syntax error in Snakefile
snakemake
updated 11 days ago by
Ram
40k • written 12 days ago by
Giuseppe Giovanni
▴ 30
0
votes
7
replies
392
views
Troubles launch IGV on Linux(Debian)
IGV
updated 4 days ago by
barslmn
★ 1.8k • written 5 days ago by
PoscaXVIII
• 0
7
votes
7
replies
3.9k
views
Genes with promoter and enhancer regions as GTF
ChIP-Seq
featurecounts
gtf
promoter
genes
updated 9 days ago by
abis.1819104
• 0 • written 5.3 years ago by
hkarakurt
▴ 170
0
votes
7
replies
621
views
What is wrong with my NCBI esearch command for 16S rRNA?
16S
NCBI
Entrez
assembly
updated 16 days ago by
GenoMax
134k • written 18 days ago by
Morgan S.
▴ 80
8
votes
7
replies
2.3k
views
CWL rerunning completed output
Common-Workflow-Language
CWL
updated 20 days ago by
Ram
40k • written 6.1 years ago by
bruce.moran
▴ 950
0
votes
7
replies
698
views
build error with vg tools
ubuntu
vg
vgtools
updated 28 days ago by
z911741
• 0 • written 12 weeks ago by
rimo
▴ 10
0
votes
7
replies
2.7k
views
qPCR: Huge variation in fold change of genes between biological replicates
qpcr
fold-change
updated 16 days ago by
Ram
40k • written 5.3 years ago by
AP
▴ 80
4
votes
7
replies
731
views
How to subtract a number from protein IDs?
blast
updated 10 days ago by
Ram
40k • written 3.2 years ago by
A_heath
▴ 140
2
votes
7
replies
2.6k
views
CWL: Getting output file of a CommandLineTool using glob
Common-Workflow-Language
cwl
updated 20 days ago by
Ram
40k • written 5.2 years ago by
skanwal
▴ 50
1
vote
7
replies
371
views
biomaRT doesn't report uniprot id
R
Uniprot
biomaRt
updated 2 days ago by
Ram
40k • written 3 days ago by
H.Hasani
▴ 990
2
votes
7
replies
5.6k
views
Specific gene labeling in a heatmap
RNA-Seq
updated 27 days ago by
GenoMax
134k • written 3.2 years ago by
clizama
• 0
3
votes
7
replies
581
views
Identifying transcription factors for a list of genes
transcription-factor
updated 6 days ago by
Ram
40k • written 4 weeks ago by
lbombini
• 0
1
vote
7
replies
3.6k
views
Adjust width of annotations in pheatmap
R
heatmap
updated 11 hours ago by
Kevin Blighe
86k • written 4.9 years ago by
divya.nandakumar
▴ 30
0
votes
7
replies
1.5k
views
what does "total read count" means in fastqc file. how does it helpful for analysis
fastqc
updated 12 days ago by
Ram
40k • written 23 months ago by
Fizzah
▴ 30
3
votes
7
replies
2.2k
views
Corrupted FASTq files with missing "+" under some sequences.
fastq
RNAseq
corruption
recover
updated 22 days ago by
Tommaso
• 0 • written 2.4 years ago by
akh22
▴ 90
7
votes
7
replies
396
views
Filter human transcription factors
transcription-factor
RNA-seq
updated 5 days ago by
jared.andrews07
★ 15k • written 6 days ago by
Chris
▴ 200
0
votes
7
replies
530
views
Highly inflated p-values in GWAS by regenie
regenie
plink
gwas
7 days ago by
cwwong13
▴ 20
4
votes
7
replies
568
views
How is the format of the bed file for samtools ampliconclip
samtools
ampliconclip
updated 26 days ago by
aw7
▴ 210 • written 4 weeks ago by
ManuelDB
▴ 70
5
votes
7
replies
446
views
Salmon index not progressing
salmon
updated 9 days ago by
Michael
53k • written 10 days ago by
camillab.
▴ 140
0
votes
6
replies
354
views
Bacterial Pangenome Analysis
Prokka
Pangenome
bacteria
Roary
4 days ago by
kirankumareripogu
▴ 10
3
votes
6
replies
11k
views
Retrieving data from NCBI GEO and RNA-Seq Data Analysis
RNA-Seq
R
GEO
Normalization
Count
updated 6 days ago by
Karma
▴ 310 • written 6.1 years ago by
hkarakurt
▴ 170
3
votes
6
replies
492
views
summarize consecutive columns over a data frame
tidyverse
r
offtopic
updated 9 days ago by
Ram
40k • written 21 days ago by
Assa Yeroslaviz
★ 1.8k
24
votes
6
replies
13k
views
How To Convert Uniprot Ids To Ensembl Gene/Transcript Ids
uniprot
updated 2 days ago by
Ram
40k • written 11.0 years ago by
learnerforever
▴ 520
8
votes
6
replies
455
views
Low Alignment rate
alignment
low_alignment_rate
Bowtie2
updated 5 days ago by
dsull
★ 4.8k • written 7 days ago by
Mehwish
▴ 10
6
votes
6
replies
5.4k
views
How is logFC and p-value estimated (for volcano plots)?
R
edgeR
fold-Change
updated 16 days ago by
Ram
40k • written 6.5 years ago by
LRStar
▴ 200
3
votes
6
replies
519
views
7 follow
How to convert data in r?
R
offtopic
updated 19 hours ago by
kalavattam
▴ 190 • written 3 days ago by
star
▴ 350
9
votes
6
replies
1.3k
views
6 follow
Forum:
GTF files from Ensembl Releases 105 and 106 unsorted
Ensembl
bug
GTF
updated 19 days ago by
ATpoint
77k • written 10 months ago by
dlaehnemann
▴ 30
2
votes
6
replies
524
views
Installing ensemble VEP by running INSTALL.pl takes too long.
Ensembl
VEP
updated 23 days ago by
Ben_Ensembl
★ 2.3k • written 25 days ago by
lsy9
▴ 20
2
votes
6
replies
312
views
obtaining circular RNAs' sequences from circBase
circular-RNA
4 days ago by
aminijavad4
▴ 10
3
votes
6
replies
326
views
Encountering Error while Running 'make vcf' in Biostar Handbook
bugfix
biostarhandbook
vcf
16 hours ago by
Tully
• 0
7
votes
6
replies
3.2k
views
CWL: word counting
Common-Workflow-Language
cwl
updated 20 days ago by
Ram
40k • written 6.3 years ago by
gultagr
▴ 10
3
votes
6
replies
650
views
BWA | SAMtools | GATK debugging help
alignment
mapping
vcf
19 days ago by
TheScriptOfGilgamesh
▴ 20
4
votes
6
replies
2.7k
views
IDT vs primer 3 - PCR dimerisation
PCR
primer3
IDT
primer
updated 17 days ago by
Emrah
• 0 • written 3.1 years ago by
timothy.kirkwood
▴ 130
1
vote
6
replies
3.3k
views
Extracting sequence, not reads, by position from bam
sequence
updated 12 days ago by
manu
• 0 • written 5.0 years ago by
geneware
▴ 10
8
votes
6
replies
552
views
Short Read Data Genome Assembly
spades
genome-assembly
updated 22 days ago by
ccstaats
▴ 30 • written 25 days ago by
Umer
▴ 50
7
votes
6
replies
10k
views
7 follow
Blastn Segmentation Fault
blast
updated 26 days ago by
Amirhossein Hajianpour
▴ 40 • written 11.8 years ago by
Srihari
▴ 30
1
vote
6
replies
605
views
Batch correction in DESeq2 - limited impact for unsupervised downstream analyses
deseq2
19 days ago by
Jane
• 0
9
votes
6
replies
317
views
Software Tool to Translate DNA to Protein
protein
DNA
fasta
updated 4 days ago by
GenoMax
134k • written 4 days ago by
sperezilvia
▴ 10
5
votes
6
replies
401
views
From where can I obtain vcf files of healthy exomes
exome
VCF
updated 26 days ago by
Ram
40k • written 27 days ago by
christinacanavati2014
• 0
7
votes
6
replies
6.1k
views
7 follow
ATAC-seq +4 -5 shift
atac-seq
updated 3 days ago by
ATpoint
77k • written 3.5 years ago by
Jingyue
▴ 70
15
votes
6
replies
1.0k
views
Annotating TSS: By Transcript or by Gene? Code Validation Help Needed!
genome
bed
transcript
TSS
6 days ago by
Rafael Soler
★ 1.2k
8
votes
6
replies
665
views
Problems using ERCC spike-ins for normalization in DESeq2
Spike-ins
Differential-expression
DESeq2
RNA-seq
19 days ago by
manuel.fernandez
▴ 40
0
votes
6
replies
570
views
How to get top 10 genes expressed in a cluster ( for cell type identification)?
seurat
18 days ago by
Chris
▴ 200
0
votes
6
replies
286
views
gene correlations in between two groups
gene-expression
correlation
5 days ago by
biology_inform
▴ 40
0
votes
6
replies
962
views
How to run delly with multi-threading mode?
SV
calling
delly
updated 12 days ago by
bio-jr
▴ 10 • written 8 months ago by
Xi
• 0
4
votes
6
replies
383
views
Hisat2 index and alignment question
ubuntu
rna-seq
index
hisat2
updated 5 days ago by
Istvan Albert
98k • written 8 days ago by
Athena
• 0
1
vote
5
replies
498
views
Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempting To Use CuffDiff
CuffDiff
Bash
Cufflinks
2 days ago by
Y
• 0
4
votes
5
replies
392
views
identify DEGs across all conditions and per specific conditions
R
DEseq2
3 days ago by
camillab.
▴ 140
3
votes
5
replies
1.0k
views
Running purge_dups on a hybrid assembly (optical mapping + reads)
pacbio
purge_dups
Assembly
bionano
updated 18 days ago by
colindaven
5.5k • written 2.7 years ago by
pablo
▴ 280
757 results • Page
3 of 16
Recent Votes
Comment: Convert amino acid sequences into nucleotide sequences
A: Translation Of Nucleotide To Amino Acid
Answer: Translation Of Nucleotide To Amino Acid
Comment: How to filter "productive" amino acid sequences
Comment: bedtools intersect by position & stand not working even with common regions
Comment: How to convert data in r?
Answer: Search RCSB with a list of protein names?
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Comment: Convert amino acid sequences into nucleotide sequences
by
sperezilvia
▴ 10
okay, I will try it. Thank you so much for your help!
Answer: Convert amino acid sequences into nucleotide sequences
by
Michael
53k
There is backtranseq, also in EMBOSS. You can choose a codon model to deal with the ambiguities in the genetic code. However, that will not…
Comment: Convert amino acid sequences into nucleotide sequences
by
sperezilvia
▴ 10
I translated all the nucleotides sequences in all 6 frames. And then, I filtered the ones with the complete reading frame (with Metionine i…
Comment: Convert amino acid sequences into nucleotide sequences
by
Joe
21k
The steps would be as follows: 1. Have nucleotide sequence fasta 2. Translatie nucleotide seqs to protein sequence fasta 3. Apply filters …
Comment: Convert amino acid sequences into nucleotide sequences
by
sperezilvia
▴ 10
sorry, how can I filter proteins without translating nucleotides into proteins? Could you give me some code sketch for this you say please?…
Comment: Convert amino acid sequences into nucleotide sequences
by
Joe
21k
If you have the nucleotides sequences, just filter the proteins, extract a list of the IDs that pass your filter, then just re-extract the …
Comment: How To Split A Bam File By Chromosome
by
Jorge Amigo
14k
`bamtools split` is intended to split the whole bam file. What you are describing is definititely not what it was designed for, and it woul…
Comment: Convert amino acid sequences into nucleotide sequences
by
sperezilvia
▴ 10
oh, I understand. I was afraid of that... In my case, I have a fasta file with multiple nucleotide sequences, which I then translated into …
Comment: Convert amino acid sequences into nucleotide sequences
by
Joe
21k
You can't *really* do this, because of the codon redundancy there is no single correct answer. That said, I did write some code to do this…
Comment: Convert amino acid sequences into nucleotide sequences
by
sperezilvia
▴ 10
Hello, I made a mistake. I just edited the question, sorry. I need to convert amino acid sequences with frames into nucleotide sequences. T…
Comment: Convert nucleotide sequences into amino acid sequences
by
Michael
53k
The sequences in your example look like amino acid sequences already. In general, you should look for the process called translation, and y…
Comment: Quality Control of VCFs that used different genotyping arrays
by
Shane
• 0
Thanks for your reply. Yes the genotyping data is in the same build. Trying to understand this: "get rid of AT,CG GC and TA SNPs". "While…
Comment: bedtools intersect by position & stand not working even with common regions
by
Alewa
▴ 130
thanks for hint!
Comment: Is it possible to predict functional profile of microbiome through 16S rRNA gene
by
safeassli
• 0
Helolo Nitin, thank you very much , However is it normal that Tax4Fun2 github repo is not awaible ? same thing for piphillin ? otherwise ba…
Answer: DEG gene list and find common TF from public ChlP-Seq data
by
Ian
6.0k
Hi. If this is human or mouse data you could try using [EnrichR][1], which is free. Try comparing against 'Transcription_factor_PPIs' and…
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