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815 results • Page
4 of 17
Sort: replies
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4
votes
4
replies
889
views
Forum:
Kubernetes in Production?
ci-cd
webservice
kubernetes
docker
updated 1 day ago by
Ram
39k • written 3.5 years ago by
acamukhin
• 0
3
votes
4
replies
279
views
Gene prediction software
pseudogene
gene
prediction
masking
updated 13 hours ago by
Darked89
4.4k • written 1 day ago by
subashini.fbtpb106
▴ 20
8
votes
4
replies
1.6k
views
News:
supporting vg users on biostars
variation-graph
genome
vg
vgteam
updated 5 days ago by
Ram
39k • written 3.7 years ago by
Erik Garrison
★ 2.4k
2
votes
4
replies
93
views
Differences in GTF files hg19 and hg38
hg38
hg19
GTF
updated 1 hour ago by
GenoMax
130k • written 5 hours ago by
heureuse
• 0
1
vote
4
replies
1.7k
views
Algorithm for clustering single cells based on SNPs?
10x-genomics
SNP
single-cell
updated 2 days ago by
Ram
39k • written 3.6 years ago by
A248
▴ 10
13
votes
4
replies
984
views
Forum:
The shortcoming of shiny app in building webserver
R
shiny
webserver
updated 5 days ago by
Ram
39k • written 3.7 years ago by
harrypotterandsbt
▴ 110
3
votes
4
replies
230
views
retaining only the clusters of interest
seurat
single-cell
scRna-seq
updated 8 hours ago by
Ram
39k • written 1 day ago by
shamza
• 0
1
vote
4
replies
39
views
How to get information about promoter from bulk-RNAseq?
promoter
R
RNA-seq
56 minutes ago by
camillab.
▴ 130
3
votes
4
replies
4.7k
views
How to get the gene expression matrix from GEO while getGEO returns 0 features?
GETquery
ATAC-seq
GEO
getGEO
RNA-Seq
updated 5 hours ago by
Ram
39k • written 3.9 years ago by
bioyas
▴ 10
1
vote
4
replies
229
views
Is hg38 on the multiz 30-way alignment inaccurate?
maf
UCSC
PHAST
multiz
2 days ago by
Daniel
▴ 30
1
vote
4
replies
466
views
best annotation approach for peaks
chipseeker
R
chippeakanno
updated 5 days ago by
rfran010
▴ 170 • written 23 days ago by
Chironex
▴ 40
6
votes
4
replies
971
views
How does long reads help in the repeated regions of the genome
Illumina
short-reads
long-reads
ONT
updated 4 hours ago by
Ram
39k • written 2.7 years ago by
Ashi
▴ 20
0
votes
4
replies
205
views
Difference between USCS exon coordinates and ensembl
exons
Ensembl
2 days ago by
amy__
▴ 50
2
votes
4
replies
263
views
Get relationships and hierarchies for GO terms
GO-terms
Gene-onthology
updated 1 day ago by
Alexander
▴ 70 • written 2 days ago by
DanielEB_fisk
▴ 10
2
votes
4
replies
823
views
Downloading all the heteroComplex protein PDB IDs in RCSB Protein Data Bank
python
biopython
updated 2 days ago by
Ram
39k • written 3.6 years ago by
Christian
• 0
0
votes
3
replies
129
views
Is PanCan data microarray or rna-seq
pancan
rna
rna-seq
microarray
data
updated 5 hours ago by
GenoMax
130k • written 15 hours ago by
survive
• 0
0
votes
3
replies
1.1k
views
Building multiple consensus sequences from multiple fasta sequences
next-gen-sequencing
sequence
alignment
updated 6 days ago by
Ram
39k • written 3.9 years ago by
miss
• 0
0
votes
3
replies
176
views
CNNScoreVariants Error
CNNscore
updated 2 days ago by
Pierre Lindenbaum
154k • written 2 days ago by
bestone
▴ 10
3
votes
3
replies
249
views
Gene enrichment analysis
Go
enrichment
r
gene
updated 2 days ago by
GenoMax
130k • written 2 days ago by
Eliza
• 0
0
votes
3
replies
326
views
(ERR): "index/Trinity.fa" does not exist or is not a Bowtie 2 index Exiting now ...
bowtie2
align_stats
trinity
3 days ago by
eimanpharmacist
▴ 20
4
votes
3
replies
726
views
What is the minimum number/threshold of gaps (NNNNNN) permitted in a draft genome?
Assembly
genome
next-gen-sequencing
updated 6 days ago by
Ram
39k • written 3.9 years ago by
Kumar
▴ 100
1
vote
3
replies
336
views
What is the benefit of knowing the source of contamination when we have RNAseq data with less than 70% mapping?
alignment
mapping
STAR
contamination
RNAseq
3 days ago by
Sib
▴ 40
0
votes
3
replies
791
views
The Tophta2 ERR, [ERRno 2]
software-error
genome
Tophat2
updated 4 hours ago by
Ram
39k • written 3.3 years ago by
ynau_kanghuadu
• 0
0
votes
3
replies
2.4k
views
Generate GTF/GFF file (coordinates) from a FASTA annotated file.
Assembly
genome
updated 5 days ago by
Ram
39k • written 3.7 years ago by
marquezg48
• 0
0
votes
3
replies
180
views
DEGseq for multiple samples
DEGseq
DEG
updated 6 days ago by
Ram
39k • written 6 days ago by
ALOUSH ALI
• 0
3
votes
3
replies
358
views
Make a BedGraph file
BedGraph
RepeatMasker
BED
2 days ago by
kirillkirilenko
▴ 20
1
vote
3
replies
933
views
News:
4th Berlin Summer School in NGS Data Analysis - October 5-9, 2020
NGS
RNA-Seq
DNA-Seq
updated 3 hours ago by
Ram
39k • written 3.3 years ago by
David Langenberger
9.9k
1
vote
3
replies
225
views
Differential protein expression analysis
R
proteomic
statistics
updated 10 hours ago by
dsull
★ 4.2k • written 4 days ago by
Ribo
▴ 40
1
vote
3
replies
194
views
How to get a comperative result of 2 bed files?
bam
cnv
bed
updated 6 days ago by
Pierre Lindenbaum
154k • written 7 days ago by
herh
• 0
3
votes
3
replies
1.3k
views
Bridge Amplification error rate and duplicates in NGS
NGS
PCR-duplicates
bridge-pcr
next-gen
updated 6 days ago by
Ram
39k • written 3.9 years ago by
KirGen
▴ 20
0
votes
3
replies
2.1k
views
Picard's Tool MarkDuplicates problem
picard
updated 4 hours ago by
Ram
39k • written 3.3 years ago by
harmadikemil
• 0
2
votes
3
replies
415
views
What is the best way to clean bulk RNA-seq data?
normalization
TPM
r
updated 5 days ago by
rfran010
▴ 170 • written 9 days ago by
JACKY
▴ 100
0
votes
3
replies
1.9k
views
METAL (gwas meta-analysis tool)
meta-analysis
gwas
metal
genomics
updated 6 hours ago by
Ram
39k • written 3.4 years ago by
bigfoot
▴ 10
3
votes
3
replies
321
views
Why is coordinate sort required before findng read depths?
wgs
sambamba
updated 2 days ago by
zhang yi xing
▴ 10 • written 5 days ago by
guntul
• 0
2
votes
3
replies
227
views
Same GEO Accession, different SRR number, how to download this RNA-seq paired-end data?
sra-toolkit
fastq
RNA-seq
SRA
updated 1 day ago by
GenoMax
130k • written 1 day ago by
ev97
• 0
0
votes
3
replies
237
views
Gviz Coverage Plots
gviz
scrnaseq
cellranger
r
updated 23 hours ago by
Ram
39k • written 1 day ago by
Researcher
• 0
4
votes
3
replies
5.4k
views
ATACseq alignment issues
BWA
alignment
ATAC-seq
updated 5 hours ago by
Ram
39k • written 7.5 years ago by
kaivalya.shevade
• 0
4
votes
3
replies
309
views
What does canonical transcript mean in the context of VEP
vep
updated 4 days ago by
Vincent Laufer
★ 2.9k • written 5 days ago by
curious
▴ 720
1
vote
3
replies
1.4k
views
Is there any tool available for sequence alignment concatenation and supermatrix file generation other than Fastconcat-G?
Assembly
alignment
software-error
updated 2 days ago by
Ram
39k • written 3.6 years ago by
Kumar
▴ 100
1
vote
3
replies
210
views
My kernel is killing the pairtools dedup script, how to prevent that?
ubuntu
hic
pairtools
aws
updated 6 days ago by
GenoMax
130k • written 6 days ago by
NikhilP
▴ 20
3
votes
3
replies
2.9k
views
Normalize BigWig's for # of reads in peaks and sequencing depth
Deeptools
ATAC-seq
updated 5 hours ago by
Ram
39k • written 3.3 years ago by
Zeel
• 0
2
votes
3
replies
1.2k
views
mutational signatures in different tumor samples
somatic mutation
mutational signature
updated 6 days ago by
Vincent Laufer
★ 2.9k • written 4.4 years ago by
lzy
▴ 20
3
votes
3
replies
352
views
Retrieving allele-specific information for a variant using VEP annotation
variant-calling
VEP
COSMIC
5 days ago by
bt_cepo
▴ 20
1
vote
3
replies
944
views
Job:
DADA2/R Programmer Needed for One-Time Job
R
microbiome
dada2
updated 2 days ago by
Ram
39k • written 3.6 years ago by
espop23
▴ 60
3
votes
3
replies
906
views
Forum:
Large Multi-omics Project -- Dev Approach
snp
RNA-Seq
sequencing
updated 5 days ago by
Ram
39k • written 3.8 years ago by
CK
▴ 10
1
vote
3
replies
722
views
Forum:
The pStacks module of Stacks has been removed?
software-error
updated 5 days ago by
Ram
39k • written 3.8 years ago by
nbarthelemy01
▴ 20
0
votes
3
replies
838
views
meta-analysis of geo dataset
R
microarray
gene
updated 7 hours ago by
Ram
39k • written 3.4 years ago by
firstorthopedicdoctor
▴ 30
1
vote
3
replies
418
views
NanoSim Error "Please specify the training reads and its reference genome!"
NanoSim
RNA-seq
LongRead
2 days ago by
akainth
▴ 10
1
vote
3
replies
133
views
Masking before RNA-Seq Alignment and Gene Prediction in Plant Genomes
RNA-evidence
genome
prediction
masking
updated 9 hours ago by
Darked89
4.4k • written 12 hours ago by
Ayish
• 0
1
vote
3
replies
188
views
Obtain a --gene-trans-map file after rnaSPAdes
rnaSPAdes
Trinity
2 days ago by
UserA
• 0
815 results • Page
4 of 17
Recent Votes
Converting Genome Coordinates From One Genome Version To Another (Ucsc Liftover, Ncbi Remap, Ensembl Api)
Converting Genome Coordinates From One Genome Version To Another (Ucsc Liftover, Ncbi Remap, Ensembl Api)
A: Mouse promoter regions
Answer: How to get information about promoter from bulk-RNAseq?
A: Extracting Flanking Regions across TSS using R scripts
A: Mouse promoter regions
A: Finding zebrafish promoters ENSEMBL
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Comment: How to get information about promoter from bulk-RNAseq?
by
camillab.
▴ 130
and what about enhancer? is it possible to do it from a bulk-RNAseq?
Comment: How to get information about promoter from bulk-RNAseq?
by
camillab.
▴ 130
yes! clearly the terminology on my side needs to improved!
Comment: How to get information about promoter from bulk-RNAseq?
by
rpolicastro
12k
Generally speaking, most promoters only initiate transcription for a single gene, with a single gene potentially having multiple promoters …
Answer: How to get information about promoter from bulk-RNAseq?
by
Trivas
★ 1.2k
I'd probably select the genes of interest from your bulk RNA-seq results then use the UCSC table browser to get the sequence upstream of th…
Comment: How to add annotation columns with specific colors to the heatmap?
by
Ram
39k
Show us your exact code - it is quite easy to tweak colors in ComplexHeatmap with the `col=` argument.
Comment: VCF file CSQ flag
by
Ram
39k
True, but it's not technically impossible to annotate GVCFs, which is why I did not address that point.
Comment: Creating a local version of Clustered NR database
by
PeterC_NCBI
▴ 260
@genomax Sorry for the delay in reply. We're still working out the details of what exactly to provide. One idea is to provide just the rep…
Comment: Shannon's Entropy by Sliding Window in R
by
rohitsatyam102
▴ 690
Hi Were u able to achieve this?
Comment: Differences in GTF files hg19 and hg38
by
GenoMax
130k
Since this is not an answer to the original question do not add it as such. I moved your last comment to the correct spot it should have be…
Comment: VCF file CSQ flag
by
Pierre Lindenbaum
154k
if your talking about the gvcf produced by HaplotypeCaller with ERC=GVCF, you don't annote g.vcf files , you must first merge the g.vcf.f…
Comment: [python]sklearn.ensemble.RandomForestClassifier(TypeError: string indices must b
by
Ram
39k
https://www.biostars.org/u/127538/ : Please delete this question or it will be deleted by moderators in a few days.
Comment: VCF file CSQ flag
by
Ram
39k
A gvcf files has blocks for both variant loci and non-variant loci, so you won't see a CSQ for each record, only for those variant loci wit…
Comment: Differences in GTF files hg19 and hg38
by
heureuse
• 0
Thank you very much. I get it from UCSC.
Comment: [python]sklearn.ensemble.RandomForestClassifier(TypeError: string indices must b
by
Arup Ghosh
3.2k
This post does not fit the theme of this forum.
Comment: CellRanger problem
by
Arup Ghosh
3.2k
Try with atleast 32 GB of Memory rather than 16 GB specified by `limitGenomeGenerateRAM` option.
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