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Limit : this year
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1,000 results • Page
2 of 20
Sort: replies
Rank
Views
Votes
Replies
44
votes
44
replies
23k
views
9 follow
bash loop for alignment RNA-seq data
RNA-Seq
next-gen
alignment
updated 8 months ago by
Ram
39k • written 9.1 years ago by
Paul
★ 1.5k
102
votes
44
replies
7.9k
views
22 follow
What Are Numbers Every Bioinformatician Should Know?
knowledge
career
updated 12 weeks ago by
Ram
39k • written 9.8 years ago by
brentp
24k
121
votes
44
replies
19k
views
21 follow
What Is Your Favorite Question To Ask When Interviewing Potential Bioinformaticians?
subjective
updated 3 months ago by
Ram
39k • written 12.9 years ago by
Madelaine Gogol
5.3k
152
votes
44
replies
29k
views
29 follow
Which Bioinformatic Friendly Pipeline Building Framework?
scripting
updated 11 months ago by
Ram
39k • written 9.4 years ago by
Carlos Borroto
★ 2.0k
196
votes
44
replies
69k
views
33 follow
Tutorial:
Analysing Microarray Data In Bioconductor
microarray-analysis
bioconductor
updated 4 months ago by
Ram
39k • written 10.7 years ago by
Obi Griffith
20k
57
votes
43
replies
14k
views
8 follow
Forum:
Best RNA-Seq aligner: A comparison of mapping tools
NGS
alignment
genome
RNA-Seq
sequence
updated 11 weeks ago by
Ram
39k • written 4.3 years ago by
David Langenberger
9.9k
170
votes
43
replies
16k
views
25 follow
Forum:
Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
updated 4 weeks ago by
zx8754
11k • written 10.2 years ago by
Istvan Albert
98k
1
vote
43
replies
2.5k
views
Database from .gbk files
database
gbk
ncbi
updated 6 weeks ago by
Raul
• 0 • written 3.0 years ago by
graysonford
▴ 10
185
votes
43
replies
79k
views
29 follow
Drawing Chromosome Ideograms With Data
chromosome
visualization
ideogram
updated 8 months ago by
Ram
39k • written 13.2 years ago by
Yuri
★ 1.6k
59
votes
43
replies
18k
views
18 follow
Tutorial:
Polish PacBio assembly with latest PacBio tools : an affordable solution for everyone
pbalign
pacbio
arrow
smrt-limk
updated 10 weeks ago by
Ram
39k • written 5.7 years ago by
Rox
★ 1.4k
138
votes
42
replies
20k
views
22 follow
Forum:
Are We Rude/Do We Expect Too Much From People Asking Questions On This Forum?
meta
updated 8 weeks ago by
Vincent Laufer
★ 2.8k • written 10.0 years ago by
Whetting
★ 1.6k
78
votes
42
replies
29k
views
25 follow
Tutorial:
Extract Total Non-Overlapping Exon Length Per Gene With Bioconductor
fpkm
rna-seq
bioconductor
updated 19 days ago by
Ram
39k • written 9.6 years ago by
Irsan
★ 7.7k
122
votes
42
replies
9.0k
views
21 follow
News:
Help Make Biostar Better! Add Your Feedback, Opinions And Suggestions.
meta
biostars
updated 3 months ago by
Ram
39k • written 10.5 years ago by
Istvan Albert
98k
8
votes
42
replies
3.8k
views
NaS (Nanopore Synthetic-long) help
Assembly
preprocessing
updated 4 months ago by
Ram
39k • written 8.0 years ago by
midox
▴ 290
202
votes
41
replies
23k
views
20 follow
Forum:
Genomics is not Special. Computational Biologists are reinventing the wheel for big data biology analysis
genomics
cram
gatk
galaxy
updated 3 months ago by
Ram
39k • written 8.5 years ago by
William
★ 5.2k
23
votes
41
replies
13k
views
10 follow
WGCNA modules and categorical traits relationship
WGCNA
updated 6 months ago by
lovelymaoqin
• 0 • written 5.4 years ago by
BrunoGiotti
▴ 110
16
votes
41
replies
7.9k
views
How to extract information from headers of fasta file
sequence
updated 11 months ago by
Ram
39k • written 8.2 years ago by
Crystal
▴ 70
13
votes
41
replies
1.7k
views
Is my file created completely
sam
BWA-MEM
BWA
updated 9 weeks ago by
Ram
39k • written 6.1 years ago by
micro32uvas
▴ 10
167
votes
41
replies
87k
views
27 follow
Multiline Fasta To Single Line Fasta
sequence
fasta
updated 3 months ago by
Onur
• 0 • written 12.0 years ago by
Palu
▴ 240
98
votes
41
replies
4.6k
views
17 follow
Forum:
I am really pissed off by the bioinformatics software world. Do/can we have a better solution?
software-error
next-gen-sequencing
updated 7 weeks ago by
Ram
39k • written 6.7 years ago by
moxu
▴ 500
23
votes
41
replies
2.5k
views
6 follow
I want to correct the erroneous barcode file, and the Python code that I've written, using Biopython, is very slow. How can I make this process fast…
NGS
biopython
illumina
WGS
updated 17 days ago by
Ram
39k • written 5 weeks ago by
Vijith
▴ 30
18
votes
41
replies
11k
views
6 follow
paired end illumina reads
Assembly
paired end
updated 10 months ago by
Ram
39k • written 7.6 years ago by
midox
▴ 290
18
votes
40
replies
16k
views
16 follow
Tutorial:
Easy way to run easily orthoMCL (Copy & paste)
all-v-all
orthomcl
updated 10 weeks ago by
Ram
39k • written 6.9 years ago by
Esaie
▴ 170
6
votes
40
replies
9.3k
views
Fastx_Collapser Has No Fastq Output
fastx
RNA-seq
updated 12 weeks ago by
Ram
39k • written 9.7 years ago by
nbvasani
▴ 240
151
votes
39
replies
23k
views
20 follow
Forum:
A Farewell To Bioinformatics
bioinformatics
updated 3 months ago by
Ram
39k • written 10.3 years ago by
Martin A Hansen
3.0k
36
votes
39
replies
2.8k
views
11 follow
Forum:
Survey/Vote: If you could double the speed of any three commandline tools, which three would they be?
blast
RNA-Seq
alignment
next-gen-sequencing
updated 10 days ago by
Ram
39k • written 5.6 years ago by
dhbradshaw
▴ 130
18
votes
39
replies
3.0k
views
Tool:
CBioInfCpp.h as a C++ lib containing some functions for bioinformatics
cpp
updated 6 days ago by
Ram
39k • written 4.1 years ago by
chernouhov sergey
▴ 50
226
votes
39
replies
282k
views
33 follow
Gene Id Conversion Tool
mapping
conversion
updated 9 months ago by
Ram
39k • written 13.6 years ago by
Renee
▴ 620
91
votes
39
replies
133k
views
19 follow
Extract Reads From A Bam File That Fall Within A Given Region
bam
updated 5 weeks ago by
Dan
▴ 120 • written 10.9 years ago by
abi
▴ 370
25
votes
39
replies
4.7k
views
8 follow
Forum:
Bioinformatics Study Group
study-group
updated 12 weeks ago by
Ram
39k • written 9.9 years ago by
Olivier
▴ 440
69
votes
39
replies
3.4k
views
14 follow
Forum:
Preparation for Bioinformatics.SE closing
meta
biostars
updated 15 days ago by
Ram
39k • written 6.0 years ago by
John
13k
277
votes
39
replies
168k
views
29 follow
Tutorial:
How to download raw sequence data from GEO/SRA
fastq
SRA
bam
GEO
updated 9 weeks ago by
Ram
39k • written 8.8 years ago by
Obi Griffith
20k
81
votes
39
replies
6.2k
views
11 follow
News:
State Of Biostar - Future Directions (January 2013)
biostars
updated 3 months ago by
Ram
39k • written 10.4 years ago by
Istvan Albert
98k
39
votes
38
replies
7.2k
views
9 follow
DNA composition - all k-mers and their frequency in some sequencing data
sequencing
updated 9 months ago by
Ram
39k • written 7.5 years ago by
John
13k
100
votes
38
replies
28k
views
34 follow
Forum:
List of cloud genomics companies
cloud-genomics
updated 20 days ago by
Jeremy Leipzig
21k • written 9.5 years ago by
14134125465346445
★ 3.6k
48
votes
38
replies
15k
views
8 follow
Forum:
Eukaryotic Genome Annotation in 2016
software
genome
annotation
updated 7 weeks ago by
Ram
39k • written 7.2 years ago by
Eric Normandeau
11k
224
votes
38
replies
136k
views
29 follow
Tool:
Converting Genome Coordinates From One Genome Version To Another (Ucsc Liftover, Ncbi Remap, Ensembl Api)
genome
coordinates
conversion
liftover
ensembl
Tool
updated 5 months ago by
harelarik
▴ 90 • written 10.2 years ago by
Malachi Griffith
19k
47
votes
37
replies
7.4k
views
12 follow
Forum:
The Organisation Of Really Contrived Acronyms (Orca)
acronyms
updated 12 weeks ago by
Ram
39k • written 9.8 years ago by
Niallhaslam
2.3k
110
votes
37
replies
7.1k
views
13 follow
Bioinformatics Career Survey 2011/2012
career
updated 3 months ago by
Ram
39k • written 11.7 years ago by
Pierre Lindenbaum
154k
43
votes
37
replies
1.7k
views
7 follow
Forum:
Checking the quality and reliability in bioinformatics
reproducibility
updated 11 days ago by
Ram
39k • written 5.6 years ago by
fi1d18
★ 4.2k
244
votes
36
replies
8.0k
views
32 follow
Forum:
Good Habit for Bioinformatics Analyst or Scientist
Analyst
Scientist
updated 7 weeks ago by
Ram
39k • written 7.1 years ago by
Shicheng Guo
★ 9.3k
49
votes
36
replies
7.4k
views
11 follow
Forum:
New features and bug fixes for Biostar to work on during Google Summer of Code
meta
biostars
gsoc
updated 3 months ago by
Ram
39k • written 9.1 years ago by
Paolo
▴ 110
251
votes
36
replies
31k
views
27 follow
Forum:
How Do You Manage Your Files & Directories For Your Projects ?
project-management
file-management
updated 9 weeks ago by
Ram
39k • written 13.1 years ago by
Pierre Lindenbaum
154k
9
votes
36
replies
10k
views
18 follow
Convert Gene IDs of Tomato Sol Database
tomato
gene
updated 5 months ago by
Ram
39k • written 7.9 years ago by
kevluv93
▴ 170
14
votes
36
replies
3.0k
views
6 follow
GPL6883_HumanRef-8_V3_0_R0_11282963_A (illumina expression beadchip)
R
updated 6 months ago by
seta
★ 1.8k • written 2.5 years ago by
daniela.paola.s.p
▴ 50
59
votes
36
replies
35k
views
27 follow
Pca From Vcf Files
pca
genome
vcf
updated 9 months ago by
hewm2008
▴ 40 • written 11.1 years ago by
Rubal7
▴ 820
148
votes
35
replies
59k
views
33 follow
Tutorial:
Tools For Metagenomic Data Analysis
metagenomics
next-gen
updated 3 months ago by
Ram
39k • written 10.5 years ago by
vijay
★ 1.6k
115
votes
35
replies
38k
views
20 follow
Forum:
Snakemake vs. Nextflow: strengths and weaknesses
snakemake
nextflow
updated 15 days ago by
Ram
39k • written 5.9 years ago by
ropolocan
▴ 760
76
votes
35
replies
26k
views
13 follow
Illumina Instrument Type from fastq?
fastq
updated 26 days ago by
Nathan
▴ 20 • written 6.9 years ago by
andrew.j.skelton73
6.5k
65
votes
35
replies
19k
views
17 follow
Which Dna Compression Algorithms Are Actually Used?
dna
updated 9 months ago by
Pierre Lindenbaum
154k • written 12.0 years ago by
Anthony Labarre
▴ 270
1,000 results • Page
2 of 20
Recent Votes
Comment: DEgs RNAseq
Answer: ScRNA data
Answer: extract all fasta sequence from db v5
Does the RNAseq data normal if the TPM value 3rd Qutile expression is near 10, but the Max expression are near 20,000
Comment: Does the RNAseq data normal if the TPM value 3rd Qutile expression is near 10, b
Answer: Does the RNAseq data normal if the TPM value 3rd Qutile expression is near 10, b
A: extract dendrogram cluster from pheatmap
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Recent Replies
Comment: Single cell chemistry
by
David
• 0
Unlikely, this data came from a experienced sequencing team, I 'm the inexperienced data analyst. I'm not sure if these samples are 10x, …
Comment: Single cell chemistry
by
David
• 0
Sorry, I did not explain it well. I did it before with other group of samples and different chemistry, so cellranger is working fine in my…
Comment: Do bioinformaticians often break molecular biologists' hearts by being the first
by
jli.ww
• 0
Based on my personal experience as a computational biologist, I was not a co-first author even if I prioritized the candidates from the pub…
Comment: DEgs RNAseq
by
Juan
• 0
Dear Delaney Sullivan, thanks, finlly I did my analisys
Answer: ScRNA data
by
Dr William Klubinski
▴ 20
It seems like your single cell RNAseq data does not already contain the Cell Barcodes or Unique Molecular Identifiers in the standard way -…
Comment: Demultiplexing bam file
by
GenoMax
129k
> Part of the header of the bam file So perhaps there are more samples. https://www.biostars.org/u/21477/: `grep "SM*" header_of_bam`. Is…
Answer: extract all fasta sequence from db v5
by
GenoMax
129k
If you need the fasta sequence download from NCBI here (146 GB zip compressed file) : https://ftp.ncbi.nih.gov/blast/db/FASTA/nr.gz
Comment: ScRNA data
by
GenoMax
129k
Is this from a SRA dataset? If so it is possible that you can get the correct information in `DataAccess` tab. Provide the accession number…
Comment: extract all fasta sequence from db v5
by
Pierre Lindenbaum
154k
is version 4 compatible with blast v5 ? try to use version 4....
Comment: Pre-processing for Agilent microarray data?
by
solarchan7
• 0
thank you!
Comment: Does the RNAseq data normal if the TPM value 3rd Qutile expression is near 10, b
by
alwayshope
▴ 20
Thanks a lot! Very helpful detailed guidance! Appreciate it a lot!
Answer: Does the RNAseq data normal if the TPM value 3rd Qutile expression is near 10, b
by
Dr William Klubinski
▴ 20
The values you are getting, with a third quartile of appx 10 and a max of appx 20,000, seem quite standard for RNA-Seq analysis. The RNA-se…
Comment: Demultiplexing bam file
by
Pierre Lindenbaum
154k
you don't talk about the size in your question
Comment: Demultiplexing bam file
by
hasani.iut6
▴ 60
so why the size of bam file is 40 gig?
Comment: Demultiplexing bam file
by
Pierre Lindenbaum
154k
> I want to separate every sample I don't understand. In your header there is only one sample : "`13.02.1402_POOL2"`
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