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99 results • Page
2 of 2
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605
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Forum:
make sense out of Whole Exome Sequencing on cancer cell lines
next-gen-sequencing
Whole-Exome-Sequencing
SNP
updated 20 minutes ago by
Ram
39k • written 3.1 years ago by
Francesco
▴ 20
0
votes
0
replies
593
views
Job:
HIRING: Data Wrangler, UCSC Genomics Institute
data-wrangler
genomics
updated 11 minutes ago by
Ram
39k • written 3.1 years ago by
genomicshr
▴ 30
0
votes
0
replies
571
views
News:
One Week Reminder for the precisionFDA Gaining New Insights by Detecting Adverse Event Anomalies Using FDA Open Data Challenge
R
updated 1 minute ago by
Ram
39k • written 3.1 years ago by
stephens_sarah
▴ 150
0
votes
1
reply
569
views
BLAST in Ubuntu
ubuntu
BLAST
updated 2 hours ago by
Ram
39k • written 3.1 years ago by
lexa_2294
• 0
0
votes
0
replies
568
views
News:
Training in Advanced Transcriptomics (RNA-Seq) Analysis@NIH, June 9-12,2020
RNA-Seq
Biomedical
next-gen
updated 2 minutes ago by
Ram
39k • written 3.1 years ago by
AABI
▴ 130
0
votes
0
replies
549
views
News:
Gene Ontology Virtual User Meeting
Ontology
GO
Enrichment
Gene-Ontology
updated 5 minutes ago by
Ram
39k • written 3.1 years ago by
laurent.albou
• 0
2
votes
0
replies
539
views
News:
Online course - Introduction to Machine Learning in R -13-16 July 2020
Machine-Learning
R
updated 2 hours ago by
Ram
39k • written 3.1 years ago by
carlopecoraro2
★ 2.3k
0
votes
0
replies
526
views
News:
Reminder for the precisionFDA Detecting Adverse Event Anomalies Using FDA Open Data Challenge
R
updated 3 hours ago by
Ram
39k • written 3.1 years ago by
stephens_sarah
▴ 150
2
votes
0
replies
525
views
News:
Tailored online courses in R
Machine-Learning
R
tidyverse
updated 3 hours ago by
Ram
39k • written 3.1 years ago by
carlopecoraro2
★ 2.3k
5
votes
7
replies
521
views
How to calculate TPM from featureCounts output
rna-seq
TPM
featurecounts
updated 18 hours ago by
rfran010
▴ 170 • written 5 days ago by
survive
• 0
0
votes
0
replies
518
views
News:
precisionFDA is launching the Truth Challenge V2: Calling Variants from Short and Long Reads in Difficult-to-Map Regions on May 1st!
gene
genome
updated 2 hours ago by
Ram
39k • written 3.1 years ago by
stephens_sarah
▴ 150
2
votes
0
replies
482
views
News:
Course online - Introduction to Deep Learning; 28th September - 1st October
Deep-Learning
Python
updated 5 minutes ago by
Ram
39k • written 3.1 years ago by
carlopecoraro2
★ 2.3k
1
vote
0
replies
462
views
Forum:
New to bioinformatics
R
Python
updated just now by
Ram
39k • written 3.1 years ago by
skusmle2013
▴ 10
2
votes
0
replies
419
views
News:
ONLINE COURSE-Advanced programming in R
R
updated 3 hours ago by
Ram
39k • written 3.2 years ago by
carlopecoraro2
★ 2.3k
1
vote
5
replies
402
views
Creating a local version of Clustered NR database
diamond
clustered-nr
blast
updated 6 hours ago by
GenoMax
130k • written 7 days ago by
Wilford203
• 0
2
votes
2
replies
397
views
General Tips On Using Optimal Alignment Flags For Specific Data
bowtie2
alignment
bwa
updated 2 hours ago by
Ram
39k • written 3.1 years ago by
blake.mcmurray
• 0
0
votes
0
replies
371
views
Calculate unique number of transcripts for each sample
R
RNA-Seq
updated 4 hours ago by
Ram
39k • written 3.2 years ago by
hannah.flintham
• 0
0
votes
6
replies
341
views
Getting same value for start and end position, "DNA methylation"
DNAmethylation
methylkit
8 hours ago by
Pankaj
• 0
1
vote
2
replies
288
views
Decoding MM and ML tags in .SAM file
Methylation
samtools
ML
MM
SAM
updated 23 hours ago by
Alex Reynolds
35k • written 17 days ago by
Ethan
• 0
1
vote
6
replies
284
views
How to get information about promoter from bulk-RNAseq?
promoter
R
RNA-seq
updated 15 hours ago by
rfran010
▴ 170 • written 1 day ago by
camillab.
▴ 130
1
vote
2
replies
251
views
If I execute "AddOrReplaceReadGroups" on a sorted and duplicate-marked .bam file, do I have to re-sort and re-mark duplicates?
picard
AddOrReplaceReadGroups
bam
read-groups
updated 4 hours ago by
Ram
39k • written 20 hours ago by
javiflaja
• 0
0
votes
2
replies
238
views
How do I calculate differential expression for RNA-seq values with the "limma" package and the "ebayes" function?
differential-expression
TPM
GSEA
PANDA
22 hours ago by
John
• 0
5
votes
8
replies
219
views
conda: command not found: gatk.
conda
gatk
2 hours ago by
bestone
▴ 20
1
vote
3
replies
219
views
Reorder GO terms using R
enrichment-analysis
GO
17 hours ago by
hellokwmin
• 0
0
votes
1
reply
202
views
Job:
DevelopMed MSCA COFUND Fellowship Programme in Precision Oncology
postdoc
training
precision-oncology
5 hours ago by
developmed
• 0
0
votes
1
reply
163
views
synteny viewer
comparative-genomics
synteny
updated 23 hours ago by
cmdcolin
★ 3.0k • written 1 day ago by
shwetamgr1
▴ 10
2
votes
3
replies
142
views
Forum:
Mapping a predicted sequence to a genome
predicted
map
alignment
sequence
updated 12 hours ago by
Mensur Dlakic
★ 23k • written 14 hours ago by
Andy
• 0
2
votes
3
replies
127
views
A question about the raw RNA-seq processing workflow
GDC
RNA-seq
STAR
Ubuntu
updated 1 hour ago by
swbarnes2
13k • written 9 hours ago by
wyt1995
▴ 10
2
votes
1
reply
118
views
where to obtain hg19 reference transcriptome in gencode
hg19
salmon
gencode
updated 22 hours ago by
GenoMax
130k • written 22 hours ago by
Josh
▴ 20
0
votes
1
reply
114
views
GSEA application not launching
GSEA
updated 22 hours ago by
GenoMax
130k • written 22 hours ago by
Jen
▴ 40
0
votes
2
replies
108
views
No @hd header returned in sam file when running bwa mem
samtools
bam
bwa
updated 4 hours ago by
Ram
39k • written 14 hours ago by
MboiTui
▴ 10
1
vote
2
replies
107
views
I don't really understand what fold mean means
coverage
updated 4 hours ago by
Ram
39k • written 7 hours ago by
ManuelDB
▴ 40
0
votes
0
replies
96
views
How are the reads assigned to genes in strand-specific RNAseq libraries?
strand-specific
strandness
RNAseq
22 hours ago by
Sib
▴ 40
7
votes
7
replies
95
views
Why does Ensembl VEP provide HGVSg for some variants and not others?
vcf
VEP
1 hour ago by
Jeremy Leipzig
21k
1
vote
1
reply
94
views
How do I specify the Recalibration_data.table for "PrintReads" in GATK 4.3, if the tool does not admit -BQSR as an option anymore?
BaseRecalibrator
-BQSR
printreads
updated 7 hours ago by
Pierre Lindenbaum
155k • written 7 hours ago by
javiflaja
• 0
1
vote
1
reply
86
views
Filtering content in heatmap to highest values
R
heatmap
ggplot2
updated 4 hours ago by
Ram
39k • written 9 hours ago by
cthangav
▴ 50
0
votes
0
replies
86
views
Changing gap open and extension penalties in muscle (or any other command line tool that works on mac, really)
multiple-sequence-alignment
updated 4 hours ago by
Ram
39k • written 21 hours ago by
sodiumnitrate
▴ 20
2
votes
1
reply
80
views
Extract variants/SNPs with T>G substitution from a VCF file
VCF
SNP
updated 4 hours ago by
Ram
39k • written 9 hours ago by
Nanu
• 0
0
votes
1
reply
79
views
What's the correct way to store bam record in a vector and free them?
htslib
updated 6 hours ago by
Pierre Lindenbaum
155k • written 8 hours ago by
Belanov
▴ 10
0
votes
1
reply
71
views
multiqc: group samples by population + boxplot ?
multiqc
1 hour ago by
Pierre Lindenbaum
155k
0
votes
1
reply
54
views
Is NA12878 Illumina Platinum set wgs or wes dataset
wgs
wes
updated 5 hours ago by
GenoMax
130k • written 5 hours ago by
guntul
• 0
1
vote
5
replies
46
views
Forum:
The .vcf format: is there a file format better suited for the era of pangenomics, and what would it be like?
VCF
file-format
genomics
updated 1 minute ago by
Matthias Zepper
3.7k • written 1 hour ago by
Vincent Laufer
★ 3.0k
0
votes
0
replies
46
views
cannot find genes with abricate and VFDB
abricate
wgs
vfdb
7 hours ago by
luciano.g
• 0
0
votes
0
replies
43
views
how to ensure that embedding for the new data is initialized based on the embedding of the existing data in UMAP?
UMAP
6 hours ago by
Raheleh
▴ 250
0
votes
4
replies
30
views
GISTIC from ASCAT
alteration
copy-number
sequencing
updated 53 minutes ago by
Ram
39k • written 2 hours ago by
Hyper_Odin
▴ 280
0
votes
4
replies
29
views
BED files
Chip-seq
BED
just now by
lela2314
• 0
0
votes
0
replies
29
views
using non-matched read counts for differential expression analysis (ncRNA)?
sRNAbench
RNA-seq
expression-matrix
sRNAtoolbox
updated 3 hours ago by
Ram
39k • written 3 hours ago by
danazeid3
• 0
0
votes
0
replies
15
views
How does kallisto genomebam handle multi mapping reads?
kallisto
updated 57 minutes ago by
Ram
39k • written 1 hour ago by
bioinfo
▴ 80
0
votes
0
replies
11
views
Why some insertions are symbolic in ALT column?
vcf
sniffles
updated 16 minutes ago by
Pierre Lindenbaum
155k • written 37 minutes ago by
Maxine
• 0
99 results • Page
2 of 2
Recent Votes
Answer: Visualizing Graph Alignment Format via minigraph
Comment: Why does Ensembl VEP provide HGVSg for some variants and not others?
The .vcf format: is there a file format better suited for the era of pangenomics, and what would it be like?
ChatGPT optimized for bioinformatics questions
Trying to use hmmer to search genes over metagenome assembled genomes
Trying to use hmmer to search genes over metagenome assembled genomes
Comment: Why does Ensembl VEP provide HGVSg for some variants and not others?
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Recent Replies
Answer: The .vcf format: is there a file format better suited for the era of pangenomics
by
Matthias Zepper
3.7k
[I can't help but be reminded of this XKCD strip][1]. 1. Disagree. In my humble opinion, there is no such thing as an ideal data forma…
Answer: ChatGPT optimized for bioinformatics questions
by
Vincent Laufer
★ 3.0k
@358a1a47 This is really timely work - two thoughts for consideration: 1) I think that a major focus here should be on devising metr…
Answer: ChatGPT optimized for bioinformatics questions
by
Ram
39k
I tested it again today, on a bit of a meta level. I asked it for its opinion of biostars and it assumed I had some random context and went…
Comment: BED files
by
Pierre Lindenbaum
155k
have a look at options `-wa` and `-wb`
Comment: BED files
by
lela2314
• 0
That is what I am doing but it does not seem to be working. I am doing: bedtools intersect -a enhancer_muscle2.bed -b chip_tfbs.bed > over…
Comment: The .vcf format: is there a file format better suited for the era of pangenomics
by
Pierre Lindenbaum
155k
shameless LLM : https://ai.tinybio.cloud/chat > The statement "VCF is the ideal file format for genomic information; no better file format…
Comment: BED files
by
Pierre Lindenbaum
155k
> I have been trying to solve what did you try ? (... and your answer will contain `bedtools intersect`)
Comment: The .vcf format: is there a file format better suited for the era of pangenomics
by
Vincent Laufer
★ 3.0k
@ram - this was as bit of a dirty tactic on my part: I stated it in the extreme to provoke disagreement. I agree with you - the irony is, d…
Comment: The .vcf format: is there a file format better suited for the era of pangenomics
by
Ram
39k
> .vcf is the ideal file format for genomic information; no better file format can be conceived whether in the era of singular linear refer…
Comment: GISTIC from ASCAT
by
Ram
39k
You can use pastebin or GitHub gist to paste more lines, maybe you're being restricted because you're a new user. Did GISTIC give you somet…
Comment: Why does Ensembl VEP provide HGVSg for some variants and not others?
by
Jeremy Leipzig
21k
you're right it's an off-by-one-error on my part. thanks
Comment: A question about the raw RNA-seq processing workflow
by
swbarnes2
13k
are files a,b and c all from the same sample? Or are you trying to align three different samples all together?
Comment: multiqc: group samples by population + boxplot ?
by
GenoMax
130k
Tagging: https://www.biostars.org/u/14100/ Rest of this line is required by biostars code.
Comment: Why does Ensembl VEP provide HGVSg for some variants and not others?
by
Vincent Laufer
★ 3.0k
@genomax thank you for clarifying that - end of an era!
Comment: Why does Ensembl VEP provide HGVSg for some variants and not others?
by
GenoMax
130k
FYI: Emily no longer works for/at Ensembl. That is the reason she has taken out `_ensembl` from her profile. https://www.biostars.org/u/23…
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