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Limit : this week
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214 results • Page
4 of 5
Sort: Views
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Views
Votes
Replies
0
votes
3
replies
194
views
ATAC-seq troubleshoot - Just Noise
ATAC-seq
updated 23 hours ago by
Ram
40k • written 1 day ago by
vk
▴ 40
0
votes
1
reply
192
views
How to create structural variants ground truth for alignment of two long-read genome assemblies?
yeast
assembly
structural-variation
SV-callers
updated 9 hours ago by
Christophe
• 0 • written 3 days ago by
Thomas
• 0
0
votes
1
reply
189
views
Add samples IDs to Seurat object when integrating different samples to do differential expression analysis
scRNA-seq
Seurat
updated 4 days ago by
Ram
40k • written 4 days ago by
Sara
▴ 230
2
votes
2
replies
188
views
Kraken2 recovering only classified reads
Kraken2
taxonomy
classification
3 days ago by
SushiRoll
▴ 110
0
votes
2
replies
180
views
Identify genes for mapped reads with combined human-7HPV genome index
star
samtools
updated 4 days ago by
Ram
40k • written 5 days ago by
r.j.lock
• 0
5
votes
2
replies
180
views
kallisto normalized TPM values without bootstraps
tpm
cibersort
sleuth
kallisto
RNA-seq
1 day ago by
butterman16
▴ 20
0
votes
3
replies
178
views
Weirdness in annotation (missing allele frequencies)
allele-frequency
gnomad
annovar
updated 23 hours ago by
Ram
40k • written 1 day ago by
Can Abdullah
• 0
1
vote
1
reply
177
views
Read Counts from BAM file
bam
samtools
qualimap
readcount
updated 3 days ago by
ATpoint
76k • written 3 days ago by
Smriti
• 0
0
votes
1
reply
177
views
Esearch, Epost, and Efetch for Large Datasets in Biopython
eutils
biopython
entrez
updated 2 days ago by
Ram
40k • written 2 days ago by
Salem
• 0
0
votes
0
replies
175
views
What metrics to use to calculate variant library evenness or uniformity or bias
library-bias
variant
updated 6 days ago by
Ram
40k • written 7 days ago by
eli_bayat
▴ 90
4
votes
0
replies
174
views
Herald:
The Biostar Herald for Wednesday, September 20, 2023
herald
2 days ago by
Biostar
2.0k
2
votes
2
replies
170
views
How to check RNAseq support for annotated genes?
gene
RNA-seq
annotation
updated 22 hours ago by
Michael
53k • written 1 day ago by
BioinfoBee
• 0
3
votes
1
reply
169
views
What is the difference between norm --multiallelics -any versus --atomize?
bcftools
updated 2 days ago by
Ram
40k • written 2 days ago by
a615ebfb
▴ 30
0
votes
1
reply
168
views
Identify parent of each read in a GAF
gaf
vgteam
vg
updated 3 days ago by
Jordan M Eizenga
▴ 360 • written 4 days ago by
cfourps
▴ 10
0
votes
1
reply
167
views
gffread outputs empty gtf file
gff
gffread
gtf
updated 3 days ago by
inedraylig
▴ 20 • written 3 days ago by
sumitra.20
• 0
0
votes
0
replies
167
views
PopGenome: there are missing regions when calculating Tajima's D per gene
PopGenome
5 days ago by
Bing
• 0
0
votes
1
reply
162
views
PyMOL: how to list all selection names?
pymol
updated 5 days ago by
Wayne
★ 1.8k • written 5 days ago by
Ondina
▴ 90
2
votes
1
reply
162
views
Best Tools and Practices for CNV Variant Calling in WES Data
CNV
WES
updated 4 days ago by
Pierre Lindenbaum
157k • written 5 days ago by
Nadav
▴ 10
0
votes
1
reply
152
views
vg rna pantranscriptome
rna
vg
updated 4 days ago by
Jordan M Eizenga
▴ 360 • written 4 days ago by
z
• 0
0
votes
1
reply
148
views
Prank checkpoint and restore?
prank
alignment
updated 3 days ago by
Ram
40k • written 3 days ago by
Pit
• 0
0
votes
3
replies
143
views
Ties in reranked list
fgsea
GSEA
updated 35 minutes ago by
alserg
▴ 840 • written 19 hours ago by
Hamza
• 0
0
votes
0
replies
142
views
Why we are using filtering >0 for up and <0 for down after TopTags() to extract de genes ids?
EdgeR
logfc
DEG
5 days ago by
Ann
▴ 10
6
votes
1
reply
142
views
How to retrieve sample informations from given ID from Sequence Read Archives?
eutils
ncbi
SRA
updated 4 days ago by
Ram
40k • written 4 days ago by
DareDevil
★ 3.3k
0
votes
1
reply
142
views
OMA in AWS cloud
OMA
AWS
updated 1 day ago by
Ram
40k • written 1 day ago by
Ksel
• 0
1
vote
1
reply
141
views
Simulate short-read RNA-seq data from long-read RNA-seq data
polyester
single-cell
simulation
short-read
long-read
updated 1 day ago by
Mensur Dlakic
★ 24k • written 1 day ago by
rhonddaskl
• 0
0
votes
1
reply
140
views
How to download genomes and proteins from JGI in bulk via the command line?
jgi
cli
updated 1 day ago by
Ram
40k • written 1 day ago by
O.rka
▴ 680
0
votes
0
replies
137
views
PRSice - Phenotype File Not Read Correctly
PRSice2
6 days ago by
V
• 0
1
vote
3
replies
136
views
Finding sequences in unannotated genomes using reference coordinates
consensus
VCF
updated 23 hours ago by
Ram
40k • written 1 day ago by
Prangan
▴ 20
0
votes
0
replies
136
views
Selecting SNPs for two-sample Mendelian randomization study/analysis
Two-sample
SNPs
Mendelian
randomization
5 days ago by
huynguyenacademia
• 0
2
votes
0
replies
132
views
the dispersion estimation of edgeR and DESeq2
estimation
dipersion
edgeR
DESeq2
4 days ago by
tommy
▴ 30
0
votes
1
reply
132
views
High amount of intronic/intergenic reads in SMARTer stranded total bulk RNAseq
RNA-seq
DNA
SMARTer
updated 23 hours ago by
Ram
40k • written 1 day ago by
Mat
▴ 60
0
votes
0
replies
132
views
Homozygous reference genotype for a GIAB genome
GIAB
IDs
reference
rs
Homozygous
6 days ago by
New2R
▴ 60
0
votes
0
replies
130
views
Illumina methylation EPIC V2 array
ewas
methylation
enrichment
missMethyl
EPIC
2 days ago by
juliviglino
• 0
0
votes
0
replies
130
views
News:
Course - GLMMs in R
GLMM
Mixed-Models
updated 4 days ago by
Ram
40k • written 5 days ago by
carlopecoraro2
★ 2.3k
0
votes
0
replies
130
views
News:
Online Course - A Practical Introduction to NGS Data Analysis - October 23-25, 2023
DNA-seq
RNA-seq
Workshop
updated 4 days ago by
Ram
40k • written 4 days ago by
David Langenberger
10k
0
votes
0
replies
128
views
News:
Introduction to Nextflow (2 seats left)
Nextflow
Workflow
Reproducibility
updated 4 days ago by
Ram
40k • written 5 days ago by
carlopecoraro2
★ 2.3k
0
votes
0
replies
127
views
News:
Genomic Prediction course
Bioinformatics
GWAS
GenomicPrediction
6 days ago by
carlopecoraro2
★ 2.3k
0
votes
0
replies
127
views
cosine simialrity of mutation signature analysis
similarity
Mutational
analysis
signature
cosine
5 days ago by
probioquestioner
• 0
0
votes
1
reply
125
views
Modify the code to take most abundant reads from a cluster and process it.
cd-hit-est
clustering
updated 3 hours ago by
Mensur Dlakic
★ 24k • written 16 hours ago by
Mohd
▴ 40
0
votes
0
replies
125
views
News:
Research Breakthrough in Identifying Viral Escape Mutations for Improved Therapeutic Design
sequence-analysis
sars-cov-2
spike-protein
updated 2 days ago by
Ram
40k • written 2 days ago by
Prem
• 0
1
vote
1
reply
122
views
How to find out what adapters to remove after FastQC of RNAseq data?
Trimmomatic
FastQC
NGS
RNA-seq
updated 23 hours ago by
Ram
40k • written 1 day ago by
ella
• 0
0
votes
0
replies
121
views
maftools - median survival time doesn't match plot
R
survival
maftools
K-M
4 days ago by
BioGuy
• 0
0
votes
0
replies
120
views
News:
Course: Data Manipulation and Visualisation with Python
Python
Data-Visualization
Data-Manipulation
updated 4 days ago by
Ram
40k • written 4 days ago by
carlopecoraro2
★ 2.3k
0
votes
0
replies
119
views
News:
Workshop - Single-Cell RNA-Seq Data Analysis: A Practical Introduction (November 8-10, 2023 in Berlin)
workshop
scRNA-Seq
RNA-Seq
single-cell
updated 2 days ago by
Ram
40k • written 2 days ago by
David Langenberger
10k
0
votes
0
replies
117
views
Data for pathview() (KEGG Pathway Map) after EdgeR
EdgeR
LogFC
pathview
KEGG
5 days ago by
Ann
▴ 10
0
votes
0
replies
112
views
Genetic distance in cM from VCF of non-reference species to run Beagle
plink
beagle
vcf
updated 4 days ago by
Ram
40k • written 4 days ago by
AndrMod
• 0
0
votes
0
replies
110
views
News:
course - Population Genomics Using Ancient DNA Data
Bioinformatics
PopulationGenomics
Genomics
aDNA
6 days ago by
carlopecoraro2
★ 2.3k
0
votes
0
replies
109
views
Current landscape of approaches to scRNA-seq with nanopore sequencers?
scRNA-seq
nanopore
single-cell
updated 1 day ago by
Ram
40k • written 1 day ago by
LauferVA
3.7k
0
votes
0
replies
108
views
High pvalues when using clusterProfiler for seurat
clusterProfiler
1 day ago by
Ahmed
• 0
1
vote
0
replies
106
views
News:
Successful NCBI NIAID Codeathon on VCF Files in SARS-CoV-2 Genomics
NCBI
Codeathon
1 day ago by
PeterC_NCBI
▴ 330
214 results • Page
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Recent Votes
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
Comment: Error in bowtie2
Recommended Tools For Alternative Splicing Detection From Rna-Seq Data
Comment: Using STAR aligner to build index of hg38
Answer: How to sort using samtools
The Biostar Handbook. A bioinformatics e-book for beginners.
The Biostar Handbook. A bioinformatics e-book for beginners.
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Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I tried the commands you recommended in the bash/command line. When I tried `find "${bam_directory}" -type f -name "*.bam" | xargs samtools…
Comment: Hugo_Symbol to Entrez ID
by
GenoMax
134k
Can you provide some examples of HUGO ID's you are unable to convert?
Comment: Using STAR aligner to build index of hg38
by
GenoMax
134k
GTF file includes gene models/annotation information. You could also use a program like `salmon/kallisto` with just human transcriptome to…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
then test each files. find "${bam_directory}" -type f -name "*.bam" | xargs samtools quickcheck file "${reference_gen…
Answer: Ties in reranked list
by
alserg
▴ 840
The problem here is not the ties, but that your gene IDs in the pathway list (ensemble human genes) does not match the names of the stats v…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I did not use the space between the `-u` flag and the `-o` flag (the blank line) but I did do what you recommended: # Run CuffDiff wit…
Answer: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
how about simply trying: ``` cuffdiff -u "${annotation_gtf}" \ -o "${output_directory}" \ -b "${reference_genome}" \ -p "${num_th…
Comment: Using STAR aligner to build index of hg38
by
Grace
• 0
Yes, I know also need a GTF file. So the GTF file will include RNA information? Thanks for your answer!!
Comment: STAR Intron Motif Script Gives Segmentation fault Error
by
Y
• 0
I will try and figure it out on my own given what you all have mentioned. Thank you for your time.
Comment: Using STAR aligner to build index of hg38
by
Amitm
★ 2.2k
Have you gone through the manual [here][1]. In addition to the genome fasta file, you would also need a GTF file (of gene annotations) to b…
Comment: Highly inflated p-values in GWAS by regenie
by
4galaxy77
2.8k
You still definitely need to include principle components as covariates, even if your data is from the same ethnicity. Not including those …
Answer: How to create structural variants ground truth for alignment of two long-read ge
by
Christophe
• 0
Hi, D-genies is using minimap2 to align both genomes and minimap2 is chaining local alignments to produce a global one. If the SV are sm…
Comment: Ties in reranked list
by
Hamza
• 0
In this case there are no results in my fgsea object unfortunately
Comment: Genes with promoter and enhancer regions as GTF
by
abis.1819104
• 0
how to generate promoters.bed and enhancer.bed files from gtf file
Comment: Deepvariant variant calling by singularity
by
jeffhsu3
• 0
I am having a similar issue was this ever resolved? Getting this error: I0922 22:22:08.794076 140356687390528 make_examples_core.py:257] O…
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