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Tutorial:
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updated 3.5 years ago by
Renesh
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Recent Replies
Comment: retaining only the clusters of interest
by
seidel
11k
Could it be that idents needs a character string instead of integers? Also, you have an extra comma in your call after c(0:16).
Comment: retaining only the clusters of interest
by
Ram
39k
`indents` is probably a factor so even though you've removed cells with those level values, the factor level still remains. Don't worry abo…
Comment: Contigs to chromosomes annotation
by
GenoMax
129k
I would still recommend using `minimap2` and aligning against reference. It should go pretty fast. You can either work with PAF (default) f…
Comment: Diff Bind Questions
by
Ram
39k
I've moved this to a comment as it does not directly answer OP's question.
Comment: Diff Bind Questions
by
Daniel
▴
30
I'm very new myself and there's plenty of people who can answer your question better than I can, but here is a resource I found extremely h…
Comment: Contigs to chromosomes annotation
by
alexandru.bologa.marian
▴
50
If the number of contigs was so small I would manually check each alignment and edit the header of each contig,inserting the corresponding …
Comment: Gviz Coverage Plots
by
Ram
39k
Do not post on multiple fora just because you want a fast response, it's plain rude. At least point to the other post so we know you're ask…
Comment: Limma returned only positive logFC values
by
melissachua90
▴
40
No the library sizes across conditions should be relatively homogenous because it's from the same dataset. I clustered the samples into sub…
Comment: Limma returned only positive logFC values
by
LChart
2.6k
It shouldn't, unless there's a misspecification in design. Is it the case that the library sizes are very different between the conditions …
Comment: Contigs to chromosomes annotation
by
Darked89
4.3k
For a pure contig to Dmel chromosome BLAST should be OK.
I am fan of LAST because it is fairly easy to convert MAF to SAM/BAM and then vi…
Comment: Contigs to chromosomes annotation
by
GenoMax
129k
How many contigs do you have? If the contigs are ~2x number of Drosophila chromosomes (meaning you have long/large contigs) then using a lo…
Comment: Contigs to chromosomes annotation
by
alexandru.bologa.marian
▴
50
Thank you for your answer!
It would be wrong just to BLAST all the contigs from the draft assembly against each reference chromosome of D.…
Comment: Limma returned only positive logFC values
by
melissachua90
▴
40
Several hundred to 10000 DEGs.
Would normalization affect logFC?
Answer: Contigs to chromosomes annotation
by
Darked89
4.3k
Not sure if it is the easiest, but you can map your new assembly to i.e. Dmel genome using LAST:
* www: https://gitlab.com/mcfrith/last …
Answer: Gene prediction software
by
Darked89
4.3k
How complete/polished is your catfish genome assembly?
Also: do you have some good quality RNA-Seq?
With a draft genome it would be…
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