updated 3 hours ago by
Lissa Cruz Saavedra
•
0
•
written 7.3 years ago by
Rox
★
1.4k
14
votes
2
replies
7.0k
views
5
votes
4
replies
612
views
20 hours ago by
kirillkirilenko
▴
40
4
votes
5
replies
263
views
3
votes
6
replies
290
views
3
votes
13
replies
622
views
3
votes
9
replies
385
views
updated 12 hours ago by
Istvan Albert
100k
•
written 2 days ago by
Κοσμάς
•
0
2
votes
4
replies
266
views
2
votes
2
replies
150
views
updated 21 hours ago by
analyst
▴
30
•
written 1 day ago by
lorena9132
•
0
2
votes
4
replies
236
views
1
vote
4
replies
401
views
1
vote
4
replies
210
views
1
vote
10
replies
823
views
6 hours ago by
DKA
▴
40
0
votes
6
replies
423
views
0
votes
2
replies
169
views
0
votes
0
replies
95
views
0
votes
0
replies
53
views
11 hours ago by
David Langenberger
11k
0
votes
1
reply
93
views
updated 10 hours ago by
Sofia
•
0
•
written 14 hours ago by
mawigoj318
•
0
0
votes
3
replies
145
views
updated 10 hours ago by
GenoMax
141k
•
written 11 hours ago by
Bertalan_Takacs
▴
90
0
votes
0
replies
58
views
0
votes
2
replies
733
views
0
votes
0
replies
48
views
8 hours ago by
hemantcnaik
•
0
0
votes
0
replies
66
views
updated 6 hours ago by
Ram
43k
•
written 11 hours ago by
glaciya2018
•
0
0
votes
2
replies
138
views
0
votes
0
replies
67
views
updated 6 hours ago by
Ram
43k
•
written 16 hours ago by
newuser2024
•
0
0
votes
1
reply
105
views
updated 6 hours ago by
Ram
43k
•
written 17 hours ago by
ahmad.sajad4541
•
0
0
votes
0
replies
63
views
updated 6 hours ago by
Ram
43k
•
written 18 hours ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
58
views
0
votes
2
replies
194
views
updated 6 hours ago by
Ram
43k
•
written 1 day ago by
v.berriosfarias
▴
140
0
votes
1
reply
111
views
updated 21 hours ago by
Mensur Dlakic
★
27k
•
written 1 day ago by
benguyarenbeyaz98
•
0
0
votes
6
replies
603
views
0
votes
2
replies
108
views
updated 3 hours ago by
Mensur Dlakic
★
27k
•
written 6 hours ago by
biochugs
•
0
0
votes
10
replies
351
views
0
votes
1
reply
68
views
updated 3 hours ago by
Pierre Lindenbaum
161k
•
written 5 hours ago by
DKA
▴
40
0
votes
0
replies
75
views
16 hours ago by
salias
•
0
0
votes
0
replies
24
views
0
votes
5
replies
261
views
0
votes
14
replies
3.4k
views
6 follow
updated 12 hours ago by
drstalinantony28
•
0
•
written 2.2 years ago by
ymj
▴
10
Recent Votes
Recent Locations •
All
South Korea, 1 minute ago
USA, 1 minute ago
United States, 4 minutes ago
United States, 4 minutes ago
Singapore, 5 minutes ago
Hong Kong, 8 minutes ago
United States, 10 minutes ago
Recent Awards •
All
Popular Question to
Simone
▴
10
Popular Question to
carlopecoraro2
★
2.5k
Popular Question to
daffodil
▴
10
Popular Question to
Russ
▴
500
Popular Question to
camillab.
▴
160
Popular Question to
pramach1
▴
40
Popular Question to
Adyasha
•
0
Recent Replies
Comment: clustalo - align milions of short seq, memory issue
by
emmanouil.a
▴
120
Hi GenoMax and Mensur Dlakic, thank you for your feedback. I'm changing approach in the way to work with a smaller number of seq.
Comment: Annovar using R package
by
Pierre Lindenbaum
161k
duplicate of your previous question: https://www.biostars.org/p/9592646/
why using R when there are shell-based tools like VEP ? slivar , …
Comment: Trouble with Pilon installation
by
Lissa Cruz Saavedra
•
0
Hi
I am trying to install the software but I didn't find the way to clone from github. Could you help me with the pipeline of instruction…
Comment: Adding CB tag to bam file
by
swbarnes2
14k
I think both these programs are just adding the same tag to every line. I don't think either cares whether it's called RG or CB
Comment: PCA plot (Suggestions Needed)
by
swbarnes2
14k
Even if this was real data, the tiny numbers for the PC1 and PC2 indicate that the differences between the samples are all absolutely tiny.…
Comment: Creating Synthetic Sequences for a ML Model
by
biochugs
•
0
Thanks for the input. I'll continue to think of more ideas that may be achievable.
Comment: PCA plot (Suggestions Needed)
by
Jeremy
▴
890
You could also try kernel PCA, which is a non-linear form of PCA, although this will be more time-consuming.
Comment: Troubleshooting RNA-seq data with DNA contamination
by
DKA
▴
40
I have unstranded RNA-seq data. Please share the tool with me, if you do not mind.
Comment: PCA plot (Suggestions Needed)
by
Mensur Dlakic
★
27k
Everything that @f1f58aed said, plus that there may be no data manipulation that will give you the clusters you expected. Sometimes there a…
Answer: Creating Synthetic Sequences for a ML Model
by
Mensur Dlakic
★
27k
It is a good idea, though I don't see how it would work in practice.
I think you will encounter at least one additional problem on top of …
Answer: PCA plot (Suggestions Needed)
by
Jeremy
▴
890
Right now, the WT samples are clustering together, but you have one mutant sample off by itself, which could be an outlier. How did you sc…
Comment: Adding CB tag to bam file
by
GenoMax
141k
@yokofakun had written this: https://www.biostars.org/p/336965/
https://github.com/ruqianl/appendCB may also be useful.
Comment: Troubleshooting RNA-seq data with DNA contamination
by
GenoMax
141k
Please post information about your tool as a `tools` category post. Don't wait/ask other users to ask you.
Comment: Adding CB tag to bam file
by
Maria
•
0
Sorry for the delay in my answer, I got sidetracked.
I am using
```samtools addreplacerg -r "@RG\tID:$cell" -o $cell.bam -h $LINE```
bu…
Answer: Troubleshooting RNA-seq data with DNA contamination
by
haibol2017
▴
10
We just developed a R package to tackle this problem.
Do you have stranded or unstranded RNA-seq data contaminated with gDNA?
If you wou…
Traffic: 1945 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6