updated 5 days ago by
Raony Guimarães
★
1.2k
•
written 12.2 years ago by
Jeremy Leipzig
21k
1.2k
votes
206
replies
50k
views
108 follow
160
votes
63
replies
76k
views
38 follow
129
votes
22
replies
52k
views
15 follow
updated 1 day ago by
Kevin Blighe
85k
•
written 5.5 years ago by
Mike
★
1.8k
129
votes
38
replies
5.9k
views
21 follow
updated 6 days ago by
Ram
39k
•
written 4.2 years ago by
Istvan Albert
98k
113
votes
53
replies
5.2k
views
16 follow
updated 6 days ago by
Ram
39k
•
written 4.2 years ago by
pet.bioinformatician
▴
170
66
votes
19
replies
47k
views
17 follow
updated 6 days ago by
Sara
▴
10
•
written 10.3 years ago by
14134125465346445
★
3.6k
34
votes
8
replies
2.2k
views
9 follow
31
votes
16
replies
1.5k
views
8 follow
updated 17 hours ago by
Ram
39k
•
written 3.6 years ago by
Antonio R. Franco
★
5.1k
27
votes
17
replies
20k
views
8 follow
21
votes
15
replies
1.5k
views
9 follow
Forum:
Working abroad without a PhD
19
votes
11
replies
1.5k
views
7 follow
updated 18 hours ago by
Ram
39k
•
written 3.7 years ago by
27atcggcta27
▴
170
17
votes
8
replies
5.1k
views
updated 4 days ago by
Jeremy Leipzig
21k
•
written 11.0 years ago by
Nikolay Vyahhi
★
1.3k
14
votes
11
replies
5.4k
views
7 follow
14
votes
5
replies
6.1k
views
Forum:
Seurat vs Monocle
updated 4 days ago by
Ram
39k
•
written 3.9 years ago by
Arup Ghosh
3.2k
14
votes
10
replies
1.6k
views
updated 4 days ago by
Ram
39k
•
written 3.9 years ago by
Shicheng Guo
★
9.3k
13
votes
4
replies
7.9k
views
13
votes
2
replies
1.2k
views
News:
Thank you Biostars!
updated 6 days ago by
Ram
39k
•
written 4.1 years ago by
Glory Basumata
▴
140
13
votes
3
replies
25k
views
13
votes
6
replies
940
views
updated 6 days ago by
Ram
39k
•
written 3.9 years ago by
David Langenberger
9.9k
13
votes
4
replies
983
views
updated 3 days ago by
Ram
39k
•
written 3.7 years ago by
harrypotterandsbt
▴
110
13
votes
25
replies
3.0k
views
7 follow
12
votes
4
replies
1.3k
views
12
votes
0
replies
1.6k
views
12
votes
2
replies
2.6k
views
12
votes
13
replies
1.8k
views
6 follow
updated 5 days ago by
Ram
39k
•
written 4.0 years ago by
Gabriel Wajnberg
▴
70
11
votes
8
replies
8.3k
views
9 follow
11
votes
13
replies
3.5k
views
6 follow
11
votes
4
replies
340
views
updated 4 days ago by
Pierre Lindenbaum
154k
•
written 5 days ago by
Mohd
▴
20
11
votes
5
replies
1.2k
views
Forum:
What bioinformatic training?
updated 5 days ago by
Ram
39k
•
written 4.0 years ago by
Peter Horbatz
▴
150
11
votes
7
replies
5.1k
views
11
votes
8
replies
1.3k
views
updated 6 days ago by
Ram
39k
•
written 4.2 years ago by
Bioinfo_2006
▴
160
11
votes
5
replies
4.2k
views
11
votes
8
replies
527
views
7 follow
updated 4 days ago by
rfran010
▴
140
•
written 5 days ago by
hashim.rana11
▴
20
10
votes
7
replies
1.4k
views
Forum:
Is trimming always better?
10
votes
15
replies
1.0k
views
updated 4 days ago by
Jeremy Leipzig
21k
•
written 3 months ago by
Maximilian Haeussler
★
1.5k
10
votes
8
replies
8.3k
views
7 follow
updated 5 days ago by
dare_devil
★
3.1k
•
written 3.4 years ago by
dec986
▴
360
10
votes
4
replies
5.4k
views
updated 6 days ago by
Ram
39k
•
written 4.1 years ago by
MEHAR ALI RAZA
•
0
9
votes
8
replies
2.6k
views
9
votes
31
replies
11k
views
6 follow
9
votes
3
replies
4.5k
views
9
votes
4
replies
3.8k
views
updated 4 days ago by
jena
▴
260
•
written 5.1 years ago by
beausoleilmo
▴
530
8
votes
7
replies
390
views
updated 4 days ago by
Vincent Laufer
★
2.9k
•
written 6 days ago by
pilargmarch
▴
100
8
votes
4
replies
1.6k
views
updated 3 days ago by
Ram
39k
•
written 3.7 years ago by
Erik Garrison
★
2.4k
8
votes
7
replies
1.1k
views
updated 18 hours ago by
Ram
39k
•
written 3.6 years ago by
pedro.raposo3
▴
20
8
votes
0
replies
1.1k
views
updated 4 days ago by
Ram
39k
•
written 3.9 years ago by
David Langenberger
9.9k
8
votes
5
replies
355
views
7
votes
6
replies
2.8k
views
updated 21 hours ago by
dre.gaudio
•
0
•
written 6.0 years ago by
msimmer92
▴
300
7
votes
13
replies
3.3k
views
8 follow
7
votes
7
replies
4.4k
views
7
votes
14
replies
558
views
Recent Votes
Recent Locations •
All
United Kingdom, just now
United States, 1 minute ago
Japan, 1 minute ago
United States, 2 minutes ago
India, 2 minutes ago
Austria, 2 minutes ago
UCLA, 3 minutes ago
Recent Awards •
All
Popular Question to
Chris
▴
100
Popular Question to
v.berriosfarias
▴
90
Teacher to
Charles Plessy
★
2.9k
Recent Replies
Comment: too many zeros in 16S rRNA amplicon sequencing data
by
zhangdengwei
▴
180
Exactly. I agree that this is due to the NovaSeq which is not very compatible with qimme2 DADA2 pipeline. I finally surrendered to other ap…
Comment: Same GEO Accession, different SRR number, how to download this RNA-seq paired-en
by
ev97
•
0
Thanks for your quick reply! How would you merge them?
Comment: Same GEO Accession, different SRR number, how to download this RNA-seq paired-en
by
Trivas
★
1.2k
My guess is that some samples were resequenced. I'd recommend just merging the respective R1 and R2s and treat them as one sample.
Comment: Get protein information from ensemblbacteria using interpro
by
sgiorgetti
▴
10
@GenoMax Apologies for my belated reply.
Agreed, the REST endpoint above supports Ensembl IDs only. Mentioned because both the initial que…
Comment: VEP tab/vcf - Different output
by
Ben_Ensembl
★
2.2k
Ah, I see. Then, no, I don't believe that this is possible with the VEP
Comment: VEP tab/vcf - Different output
by
Jeremy Leipzig
21k
I don't think that is in VCF-style coordinates which show deletions like chr1:99:AG -> A instead of chr1:100:G -> -
Comment: Help with running ATAC using Encode pipeline
by
Chris
▴
100
Sure, here is my json file, I have 8 fastq files:
{
"atac.title" : “atac)”,
"atac.description" : “Encode”,
…
Comment: Differential protein expression analysis
by
dsull
★
4.2k
Yes, you need to log-transform.
I've had a good experience using limma for proteomics analyses (limma uses a moderated t-test).
Answer: Assigned taxonomy after dada2 only "NAs" or "Mitochondria"
by
mattze731
▴
10
Never mind. Someone had put all the 16S files into the ITS folder and vice versa. After using the 16S dada2 pipeline on the actual 16S file…
Comment: Getting Pairwise Sequence Alignment Score With Biopython
by
Jonathan Lefebre
▴
70
Dear Alexander,
to answer 2) first: This is just a fasta file that you can obtain essentially from every multiple sequence alignment soft…
Comment: Stratified sample assignment for analysis
by
ATpoint
72k
Batches for what? Processing/sequencing, what data are we talking about? If this is indeed paired then you basically must keep samples of a…
Answer: Visualizing Graph Alignment Format via minigraph
by
colindaven
4.8k
That's a really good question. Short version: not much to my knowledge, aside from looking at it, or filtering it (as it's a PAF like forma…
Comment: Alignment of case vs. control from different origin
by
i.sudbery
17k
BTW - Most TCGA cancer types do have normals amoungst the samples.
Comment: Alignment of case vs. control from different origin
by
i.sudbery
17k
The assumption of these studies is that any batch effect within a batch affects both treatment and control equally, so that when you divide…
Answer: Stratified sample assignment for analysis
by
Vincent Laufer
★
2.9k
First, you didn't tell us what kind of data you are working with. For arguments sake, I'll assume bulk RNA-seq.
Generally, your options …
Traffic: 2761 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6