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34 results • Page
1 of 1
Sort: Votes
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25
votes
13
replies
25k
views
8 follow
Bowtie2 parameters for best alignment
alignment
Assembly
genome
SNP
updated 14 hours ago by
kalavattam
▴ 90 • written 8.8 years ago by
cvu
▴ 180
7
votes
6
replies
312
views
6 follow
split fasta file to train deep learning model
fasta
split
bash
R
updated 3 hours ago by
shenwei356
7.9k • written 18 hours ago by
pinheirofabiano
▴ 10
5
votes
3
replies
253
views
Where do find virulence gene information in a gff/gtf file?
microbial
genomics
gff
annotation
gtf
updated 5 hours ago by
oschwengers
▴ 90 • written 1 day ago by
sovrappensiero
▴ 60
5
votes
3
replies
341
views
News:
March 13-16: Virtual Nextflow and nf-core training event in 5 languages
nf-core
pipeline
Nextflow
updated 4 hours ago by
Maxime Garcia
▴ 220 • written 4 weeks ago by
Matthias Zepper
3.3k
5
votes
6
replies
413
views
Nextflow: No space left on device, process using output directory as input?
nextflow
updated 2 hours ago by
Maxime Garcia
▴ 220 • written 2 days ago by
Eliveri
▴ 320
4
votes
7
replies
498
views
Calculate RPKM
RPKM
12 hours ago by
Chris
▴ 70
3
votes
7
replies
2.2k
views
7 follow
Converting an output de-novo transcriptome assembled with Trinity to a .gff3 file
Trinity
updated 1 hour ago by
EkHe
• 0 • written 3.5 years ago by
Raito92
▴ 80
3
votes
8
replies
5.7k
views
Help with designing limma contrast matrix
limma
software error
updated 22 hours ago by
Kevin Blighe
84k • written 5.0 years ago by
fi1d18
★ 4.1k
2
votes
4
replies
448
views
msigdbr returns the same genesets for mouse as in for human,
msigdbr
14 hours ago by
e.r.zakiev
▴ 30
2
votes
2
replies
1.8k
views
BWA alignment score calculation
score
SAM
BWA
alignment
updated 17 hours ago by
kmzhou4
• 0 • written 21 months ago by
Donovan
▴ 20
2
votes
2
replies
175
views
FastQ file not recognized by hisat2 allignment
hisat2
rnaseq
rna-seq
nextflow
updated 4 hours ago by
Maxime Garcia
▴ 220 • written 15 hours ago by
selenaajohn
• 0
1
vote
2
replies
180
views
Handling single-sample VCF after haplotypecaller
VCF
13 hours ago by
Y.H.P
• 0
1
vote
2
replies
156
views
FeatureCounts >edgeR > GO
RNA-SEQ
14 hours ago by
Pegasus
▴ 80
1
vote
2
replies
257
views
Human Population Reference Genomes (+Neanderthal?)
comparative
genomics
15 hours ago by
emm008
• 0
1
vote
1
reply
181
views
What is the difference between agilent_wholegenome and agilent_wholegenome_4x44k_v1 in biomart?
bioinformatics
biomaRt
biomart
genetics
gene
updated 4 hours ago by
Mike Smith
★ 2.0k • written 1 day ago by
solarchan7
• 0
0
votes
0
replies
6
views
Converting Abricate output (.tsv) to gff3 format
gff3
convert
tsv
Abricate
30 minutes ago by
ghataksnd
▴ 10
0
votes
2
replies
15
views
how to get pfam id of proteins belonging to a particular family along with sequences in that family?
pfam
protein
id
interpro
sequence
updated 33 minutes ago by
GenoMax
126k • written 41 minutes ago by
max
• 0
0
votes
0
replies
9
views
How to calculate the Z value of infernal cmscan?
cmscan
ncRNA
infernal
28 minutes ago by
shelvenlone
• 0
0
votes
0
replies
102
views
MethylR on bam files Gives Error
MethylR
bismark
23 hours ago by
anasjamshed
▴ 120
0
votes
4
replies
37
views
samtools idxstats not removing ChrM
samtools
ChIPseq
updated just now by
Pierre Lindenbaum
153k • written 1 hour ago by
Jen
▴ 40
0
votes
0
replies
102
views
TCGA Methylation Data and Gene Mapping
Methylation
Cancer
TCGA
21 hours ago by
James
▴ 30
0
votes
5
replies
485
views
Bad quality fastq files for analysis
quality
bad
DNA-seq
fastq
alignment
updated 20 hours ago by
GenoMax
126k • written 2 days ago by
Gene_MMP8
▴ 220
0
votes
1
reply
175
views
just one plot appears in the shiny application not all
plot
source
shiny
code
updated 15 hours ago by
bkleiboeker
▴ 310 • written 1 day ago by
mrashad
▴ 60
0
votes
0
replies
77
views
How would I assess evolutionary estimates between duplicated vs. non-duplicated genes in PAML?
PAML
12 hours ago by
codoncity
• 0
0
votes
0
replies
196
views
Simes method
simes
method
SNP
R
7 hours ago by
Eliza
▴ 10
0
votes
2
replies
137
views
GIAB Truth Set
GIAB
6 hours ago by
sheryl
▴ 180
0
votes
0
replies
74
views
got error while running Virusfinder, Please find the attached image for the details of the error, also blat_out_candidate_singlelane.fa is 0 in size.…
Step2
Virusfinder
4 hours ago by
DC
• 0
0
votes
0
replies
78
views
Fine mapping with GWAS
PAINTOR
GWAS
FINE-MAP
POST-GWAS
updated 3 hours ago by
Nicolas Rosewick
10k • written 7 hours ago by
Swati
• 0
0
votes
2
replies
79
views
UnicodeDecodeError: 'utf-8' codec can't decode byte 0x8b in position 1: invalid start byte
vcf.gz
UnicodeDecodeError
ancIBD
3 hours ago by
wangjincheng
• 0
0
votes
2
replies
95
views
Trying to install Roary with Conda results in error message
error
roary
conda
3 hours ago by
tobiwan
• 0
0
votes
3
replies
370
views
Practical Haplotype Graph -- Pathing Error
PHG
updated 2 hours ago by
lcj34
▴ 220 • written 25 days ago by
wcs98
• 0
0
votes
2
replies
186
views
Nextflow Stringtie: command not found Error
nextflow
rna-seq
stringtie
updated 1 hour ago by
ATpoint
70k • written 1 day ago by
selenaajohn
• 0
0
votes
0
replies
18
views
samtools mpileup - bases string explanation
samtools
mpileup
1 hour ago by
igorm
▴ 20
0
votes
2
replies
67
views
Problem in converting SAM to BAM file by DROP SEQ
SAMtoBAM
updated 40 minutes ago by
benformatics
3.4k • written 3 hours ago by
VANSHIKA
• 0
34 results • Page
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Recent Votes
Answer: What is the difference between agilent_wholegenome and agilent_wholegenome_4x44k
C: Converting an output de-novo transcriptome assembled with Trinity to a .gff3 fil
unplaced, unlocalized, ... scaffolds from hg19 to GRCh38
C: extracting heterozygous snp from a vcf file
Comment: March 13-16: Virtual Nextflow and nf-core training event in 5 languages
Answer: Where do find virulence gene information in a gff/gtf file?
Comment: split fasta file to train deep learning model
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Recent Replies
Answer: samtools idxstats not removing ChrM
by
Jen
▴ 40
![enter image description here][1] I think I got it to work using "MT" rather than "ChrM" (code below) samtools idxstats chipse…
Answer: how to get pfam id of proteins belonging to a particular family along with seque
by
GenoMax
126k
Visit: https://www.uniprot.org/uniprotkb?query=(taxonomy_id:9606) Click on `customize columns`. Search with keyword `pfam` and check the…
Comment: how to get pfam id of proteins belonging to a particular family along with seque
by
Pierre Lindenbaum
153k
use the data in the download section ? $ wget -qO - "https://ftp.ebi.ac.uk/pub/databases/interpro/current_release/protein2ipr.dat.gz" …
Comment: Problem in converting SAM to BAM file by DROP SEQ
by
benformatics
3.4k
i think picard is expecting read groups. you may need to add them: https://gatk.broadinstitute.org/hc/en-us/articles/360037226472-AddOrRepl…
Comment: samtools idxstats not removing ChrM
by
benformatics
3.4k
i'm not sure this is doing what you expect? you should try filtering your bam directly and then running idx stats samtools view -h yo…
Comment: samtools idxstats not removing ChrM
by
Jeremy Leipzig
21k
can we see the output from this? `samtools idxstats chipseq/results/bowtie2/PPARg/PPARg_Input_Rep1_aln_sorted.bam` conversely just as an e…
Comment: Nextflow Stringtie: command not found Error
by
ATpoint
70k
It's bad practice to delete posts that received an answer ( = a user invested time - in this case me but it of course applies generally). D…
Comment: Converting an output de-novo transcriptome assembled with Trinity to a .gff3 fil
by
EkHe
• 0
Hi @juke34, if you're able, can you please clarify something for me (based on the answer you've given here)? Thanks a lot in advance. I als…
Answer: Practical Haplotype Graph -- Pathing Error
by
lcj34
▴ 220
FYI - The latest PHG docker version (tag 1.4) has a fix for this issue.
Comment: Nextflow: No space left on device, process using output directory as input?
by
Maxime Garcia
▴ 220
Nextflow should only link inputs (unless stated otherwise), so even if you use outdir as input it should not be much of an issue, but still…
Comment: Problem in converting SAM to BAM file by DROP SEQ
by
lieven.sterck
14k
Hi https://www.biostars.org/u/126271/, welcome. it might be good to add some more info on your "question". Now it only shows an error mes…
Comment: Trying to install Roary with Conda results in error message
by
tobiwan
• 0
Thank you for your answer I'm using Ubuntu 22.04.2 LTS, command line used: conda install roary. Or do you need a screenshot of the whole …
Comment: Nextflow: No space left on device, process using output directory as input?
by
colindaven
4.5k
Yes, don't use the outdir as an input. You might get infinite loops.
Comment: UnicodeDecodeError: 'utf-8' codec can't decode byte 0x8b in position 1: invalid
by
wangjincheng
• 0
I tried unzipped file, it shows the same error
Comment: UnicodeDecodeError: 'utf-8' codec can't decode byte 0x8b in position 1: invalid
by
Pierre Lindenbaum
153k
> use python package to load and analysis vcf.gz files looks like it doesn't like gzipped file. Are you sure this software can read gzip…
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