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201 results • Page
3 of 5
Sort: Votes
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Views
Votes
Replies
1
vote
0
replies
127
views
rMATS visualisation
R
rMATS
maser
updated 1 day ago by
Ram
40k • written 2 days ago by
Smriti
▴ 10
1
vote
1
reply
214
views
Imputation server failing to see samples in VCF files
imputation
VCF
updated 4 days ago by
Ram
40k • written 4 days ago by
Ben
• 0
1
vote
7
replies
412
views
biomaRT doesn't report uniprot id
R
Uniprot
biomaRt
updated 4 days ago by
Ram
40k • written 4 days ago by
H.Hasani
▴ 990
1
vote
4
replies
253
views
How to filter vcf file by MAF using bcftools?
bcftools
vcf
updated 6 days ago by
Ram
40k • written 6 days ago by
_quantum_girl_
▴ 10
1
vote
4
replies
259
views
Running Rockhopper from a command-line
rockhopper
java
6 days ago by
langziv
▴ 50
1
vote
5
replies
256
views
How to filter "productive" amino acid sequences
protein
fasta
updated 2 days ago by
GenoMax
135k • written 5 days ago by
sperezilvia
▴ 10
1
vote
3
replies
280
views
comparision of umap single cell
single-cell
5 days ago by
synat.keam
▴ 80
1
vote
1
reply
156
views
tensorQTL interaction issue
genomics
eqtl
genetics
qtl
57 minutes ago by
Solal
• 0
1
vote
2
replies
553
views
convert bed12 to sorted gtf
bed12
gtf
gff
awk
updated 15 minutes ago by
alejandrogzi
▴ 30 • written 14 months ago by
maria
• 0
1
vote
2
replies
300
views
OMA in AWS cloud
OMA
AWS
updated 6 days ago by
Adrian Altenhoff
★ 1.1k • written 12 days ago by
Ksel
• 0
1
vote
3
replies
1.1k
views
GenotypeGVCF too many genotypes from pooled samples
GenotypeGVCFs
GATK
VCF
updated 1 day ago by
Paula Andrea
• 0 • written 2.4 years ago by
Vic
▴ 90
1
vote
4
replies
868
views
Do you need a genome or can a transcriptome alone be used to identify biosynthetic pathways? Why sequence and assemble a genome?
de-novo-assembly
biosynthetic-pathways
plant
transcriptomics
updated 1 day ago by
Ram
40k • written 21 months ago by
kristina.mahan
▴ 160
1
vote
4
replies
408
views
How to perform hypothesis testing on contingency tables and compare with the null distribution?
hypothesis-testing
contingency-table
4 days ago by
RK
• 0
1
vote
3
replies
920
views
Genewiz gave me bam files instead of fasta files- can I convert bam to fasta to have a genome assembly?
bam
samtools
fasta
updated 1 day ago by
Ram
40k • written 2.1 years ago by
kristina.mahan
▴ 160
1
vote
4
replies
1.5k
views
How do you add gene annotations from a Diamond Blastx tsv file to a csv file with RNAseq differential expression data?
BLASTx
RNA-seq
diamond
updated 1 day ago by
Ram
40k • written 2.2 years ago by
kristina.mahan
▴ 160
1
vote
1
reply
226
views
Comparing multiple RNASeq studies
RNASeq
updated 5 days ago by
Zhenyu Zhang
▴ 980 • written 5 days ago by
Luke
▴ 10
0
votes
0
replies
58
views
DMRcate design
R
Epigenetics
EPIC
DMRcate
updated 9 hours ago by
Ram
40k • written 11 hours ago by
Bioinfonext
▴ 440
0
votes
0
replies
62
views
Detection of CNVs with copy number greater than 4 by PennCNV
copy-number
PennCNV
updated 9 hours ago by
Ram
40k • written 14 hours ago by
yamamoto.yasuyuki.n6
• 0
0
votes
0
replies
68
views
Algorithmic Solutions for Resolving Overlapping Sequences in Sanger Sequencing of PCR Segments
Sanger
pcr
updated 9 hours ago by
Ram
40k • written 19 hours ago by
Amior
• 0
0
votes
0
replies
57
views
Adding means cluster from fviz_cluster on the PCA
fviz_cluster
updated 8 hours ago by
Ram
40k • written 11 hours ago by
ayaosama2111
▴ 10
0
votes
2
replies
153
views
I need help with univariate logistic regression in a set of microarray data
R
microarray
odds-ratio
updated 8 hours ago by
Ram
40k • written 22 hours ago by
Pooria
• 0
0
votes
2
replies
118
views
Get discordant read pairs from bam
samtools
NGS
updated 7 hours ago by
benformatics
3.8k • written 9 hours ago by
paulaotero.sanchez
• 0
0
votes
3
replies
287
views
QC of genetic data
PLINK
updated 7 hours ago by
bk11
★ 1.4k • written 4 days ago by
kl
▴ 10
0
votes
4
replies
285
views
Sequence quality drops in the 3' end of the left sequence in paired end. What could be the cause?
sequencing
updated 6 days ago by
GenoMax
135k • written 6 days ago by
eggrandio
▴ 40
0
votes
3
replies
193
views
Bug of vg surject
vg
updated 6 days ago by
Jordan M Eizenga
▴ 360 • written 6 days ago by
Qi
• 0
0
votes
2
replies
151
views
Generating a 3D model from 2D slices of a nucleus
imaging
modeling
6 hours ago by
jachimowicz9002
▴ 10
0
votes
1
reply
143
views
Calculating haplotype and nucleotide diversity with deep amplicon sequencing data
haplotype
diversity
Pegas
Arlequin
HTS_data
6 days ago by
deorugz
• 0
0
votes
0
replies
47
views
Adding custom annotation to VEP output VCF
bcftools
VEP
vcf
5 hours ago by
avelarbio46
▴ 30
0
votes
0
replies
33
views
Help on dendrimer building
dendrimer
gromacs
molecular-dynamics
4 hours ago by
v.berriosfarias
▴ 130
0
votes
1
reply
68
views
WGCNA plotEigengeneNetworks error (coercion to logical)
WGCNA
plotEigengeneNetworks
RNA-seq
RStudio
updated 4 hours ago by
Ram
40k • written 5 hours ago by
Victor
• 0
0
votes
0
replies
41
views
News:
Course :MACHINE LEARNING - A HANDS-ON INTRODUCTION IN R
R
Statistical-Learning
Omics
Machine-Learning
updated 4 hours ago by
Ram
40k • written 6 hours ago by
carlopecoraro2
★ 2.4k
0
votes
1
reply
450
views
Low percentage of 'Fraction Antibody Reads Usable' in Feature Barcode Cell Ranger output
scCITE-seq
feature-barcode
10x
scRNA-seq
updated 4 hours ago by
Ram
40k • written 5 months ago by
rocio.castellanos
• 0
0
votes
1
reply
221
views
Determine kit used for library preparation for whole exome sequencing from fastq files
Agilent
NGS
library-preparation
updated 4 hours ago by
Ram
40k • written 19 hours ago by
Harinder
• 0
0
votes
9
replies
224
views
Deseq2 error
R
deseq2
3 hours ago by
sgadila
• 0
0
votes
4
replies
102
views
Understanding mother and father alleles in VCF file
snp
vcf
3 hours ago by
c.
• 0
0
votes
2
replies
1.4k
views
Converting hdf5 file to loom file
single-cell
python
scvelo
RNA
updated 5 days ago by
bk11
★ 1.4k • written 2.4 years ago by
sidrah.maryam
▴ 50
0
votes
1
reply
104
views
Differentially Expressed Genes between two conditions (scRNA, single GEO dataset with multiple samples and no cell annotations)
scRNA-seq
RNA-Seq
Seurat
updated 3 hours ago by
bk11
★ 1.4k • written 4 hours ago by
prietto
• 0
0
votes
1
reply
43
views
Kimura% of calcDivergenceFromAlign output
repeatlandscape
repeatmasker
2 hours ago by
睿紘
• 0
0
votes
3
replies
726
views
to annotate BEDPE files
bedtools
annotate
updated 6 days ago by
Lhl
▴ 760 • written 2.6 years ago by
Bogdan
★ 1.4k
0
votes
0
replies
158
views
Convert from limma voom normalized matrix each gene to high/low
limma-voom
RNA-SEQ
updated 5 days ago by
Ram
40k • written 5 days ago by
Manuel Sokolov Ravasqueira
▴ 100
0
votes
0
replies
14
views
ATAC-Seq and RPKM
RPKM
ATAC-Seq
and
1 hour ago by
qudrat.nii
• 0
0
votes
10
replies
231
views
Rockhoppper - Suspiciously low percentage of perfectly aligned reads
Rockhopper
RNA-seq
1 hour ago by
langziv
▴ 50
0
votes
0
replies
151
views
Can optimal cofactors for arcsinh transformation be calculated in parallel?
parallel
R
flowcytometry
flowCore
flowVS
5 days ago by
gmiller
• 0
0
votes
0
replies
150
views
Is the concept of a cofactor in an arcsinh transformation specific to Flow Cytometry?
FlowCytometry
flowCore
FlowVS
5 days ago by
gmiller
• 0
0
votes
1
reply
197
views
Issue with merging in plink and eigensoft.
Eigensoft
Eigenstrat
Plink
updated 5 days ago by
bk11
★ 1.4k • written 5 days ago by
Jd
• 0
0
votes
1
reply
192
views
Lower alignment rate when using collapsed reads
short_rna
RNA-Seq_mapping
collapsed_reads
updated 4 days ago by
LChart
3.4k • written 5 days ago by
blz
▴ 30
0
votes
0
replies
140
views
Guide for ICD-10 to EFO conversion?
icd10
efo
5 days ago by
optimistsso4co3
▴ 100
0
votes
0
replies
137
views
Software for chimera detection of amplicon (e.g. 16S) nanopore reads
chimera
nanopore
amplicon
5 days ago by
rob_DNA
▴ 10
0
votes
3
replies
222
views
What is the correct procedure to generate a consensus bacterial sequence?
consensus
samtools
illumina
fastq
4 days ago by
marongiu.luigi
▴ 690
0
votes
1
reply
536
views
Recommendations for extending contigs from denovo assembly to identify SV insertion sites on chromosome
Nanopore
WGS
Long-Read
SV
Assembly
4 days ago by
dk0319
▴ 70
201 results • Page
3 of 5
Recent Votes
Answer: tensorQTL interaction issue
Answer: Script for getting summary statistic of any genome using GTF or GFF3 ?
Comment: How do you validate and verify your pipeline's software updates?
Comment: How do you validate and verify your pipeline's software updates?
Answer: GSEA with GO and KEGG datasets
GSEA with GO and KEGG datasets
Answer: Exporting DEGs obtained from DESeq2
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Recent Replies
Answer: Converting from BED to SAF/GFF
by
alejandrogzi
▴ 30
I recently developed [bed2gff](https://github.com/alejandrogzi/bed2gff) to quickly convert .bed files to a gff3 format, a tool written in R…
Comment: Convert bed12 to GFF
by
alejandrogzi
▴ 30
Now there is [bed2gff](https://github.com/alejandrogzi/bed2gff), if you want to work only with a gff file!
Comment: Converting different annotation file formats (GTF/GFF/BED) to each other
by
alejandrogzi
▴ 30
now there is also [bed2gff](https://github.com/alejandrogzi/bed2gff) if you want to convert .bed files to .gff3 files!
Answer: How Do I Convert From Bed Format To Gff Format?
by
alejandrogzi
▴ 30
I recently developed [bed2gff](https://github.com/alejandrogzi/bed2gff) to quickly convert .bed files to a gff3 format, a tool written in R…
Answer: tensorQTL interaction issue
by
Solal
• 0
Writing a solution for anyone interested. The issue stems from a dosage error, some variant contains only heterozygous individuals and no h…
Answer: The Biostar Handbook. A bioinformatics e-book for beginners.
by
Qboy
• 0
Great book! I love using it to help my mentees to learn bioinformatics better. It would be also fantastic to compile all other necessary Pr…
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
Thanks. I'll try.
Comment: How to get sample names and genotype for SNP in multi-sample VCF file
by
avelarbio46
▴ 30
Hi! I'm trying to parallelize your code (to run on a bunch of files), but it is not working because of multiple processes spawning. Do you …
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
GenoMax
135k
> so I don't think the genome size is available. Perhaps not for your specific strain but plenty of other Kpn genomes are available and th…
Comment: Kimura% of calcDivergenceFromAlign output
by
睿紘
• 0
For additional information, "Coverage for each repeat class and divergence (Kimura)" is also the result from calcDivergenceFromAlign.pl
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
By the way, I run Rockhopper on the untrimmed files, and the full match percentage grew to 20%-30%, and there are 1552 transcripts in the u…
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
Here are the total bases in 4 fastq files (after adapter removal): 1,027,852,411, 1,041,895,083, 1,121,786,520, 1,299,721,929. I just rem…
Comment: Understanding mother and father alleles in VCF file
by
c.
• 0
actually they didn't tell me which relatives were from mother side and which from father side, you are right. But how can you find related …
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
GenoMax
135k
Correct. Data that went into the assembly.
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
Total bases (number of reads * read length) should be calculated after removal of adapters, right?
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