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1,000 results • Page
2 of 20
Sort: Rank
Rank
Views
Votes
Replies
1
vote
1
reply
157
views
Is it possible to convert 3 prime sequencing read counts into TPMs?
RNAseq
TPM
expression
updated 2 days ago by
Trivas
★ 1.5k • written 2 days ago by
Mat
▴ 60
2
votes
5
replies
276
views
Mapping SNP rsIds/ positions to genes coding for proteins
SNP
rsid
1 day ago by
Harsh
• 0
0
votes
0
replies
112
views
Select minimum set of SNPs from VCF file to identify a individual
selection
SNP
2 days ago by
Bioxbio
• 0
1
vote
5
replies
490
views
Number of accessible regions included by DESeq2 to generate the PCA plot with the nf-core ATACseq pipeline ?
nf-core
atac-seq
PCA
DESeq2
updated 1 day ago by
Michael Love
★ 2.5k • written 2 days ago by
Alexandre Eeckhoutte
• 0
5
votes
3
replies
214
views
Where to find info on VCF file format?
bcftools
vcf
updated 2 days ago by
Ram
40k • written 2 days ago by
_quantum_girl_
▴ 10
0
votes
2
replies
220
views
ptt and rnt files that were created from assembly rather than a genome
rockhopper
genbank
2 days ago by
langziv
▴ 50
0
votes
0
replies
95
views
How to obtain de pvalue of my DMRs using Bumphunter function from minfi
DMRs
2 days ago by
Irene
• 0
1
vote
4
replies
220
views
How to filter vcf file by MAF using bcftools?
bcftools
vcf
updated 2 days ago by
Ram
40k • written 2 days ago by
_quantum_girl_
▴ 10
9
votes
6
replies
297
views
Software Tool to Translate DNA to Protein
protein
DNA
fasta
updated 2 days ago by
GenoMax
134k • written 2 days ago by
sperezilvia
▴ 10
1
vote
2
replies
294
views
OMA in AWS cloud
OMA
AWS
updated 2 days ago by
Adrian Altenhoff
★ 1.1k • written 8 days ago by
Ksel
• 0
0
votes
3
replies
203
views
What is the correct procedure to generate a consensus bacterial sequence?
consensus
samtools
illumina
fastq
22 hours ago by
marongiu.luigi
▴ 690
0
votes
2
replies
179
views
Detect STRs in illumina library
repeats
annotation
STR
2 days ago by
kirillkirilenko
▴ 20
3
votes
3
replies
226
views
Fetch Fastq files directly for SRA data
NCBI
FASTQ
SRA
updated 3 days ago by
GenoMax
134k • written 3 days ago by
Chintan
• 0
0
votes
2
replies
166
views
chromosome location
visualization
updated 2 days ago by
Ram
40k • written 3 days ago by
Farzana
• 0
1
vote
3
replies
318
views
How to determine the total count for each gene in lymphotype B
scRNAseq
Seurat
1 day ago by
dalibenam64
• 0
0
votes
4
replies
227
views
Sequence quality drops in the 3' end of the left sequence in paired end. What could be the cause?
sequencing
updated 3 days ago by
GenoMax
134k • written 3 days ago by
eggrandio
▴ 40
15
votes
16
replies
719
views
ncbi error report log for validate fastq issue
sra-tools
updated 2 days ago by
GenoMax
134k • written 3 days ago by
1769mkc
★ 1.1k
1
vote
4
replies
245
views
Running Rockhopper from a command-line
rockhopper
java
3 days ago by
langziv
▴ 50
2
votes
4
replies
275
views
Which function is best for pathway analysis?
pathway
2 days ago by
Chris
▴ 200
0
votes
3
replies
177
views
Bug of vg surject
vg
updated 3 days ago by
Jordan M Eizenga
▴ 360 • written 3 days ago by
Qi
• 0
0
votes
1
reply
154
views
GO ENRICHMENT ANALYSIS- DESEQ
GO
updated 3 days ago by
Basti
★ 1.7k • written 3 days ago by
oduduabasi.isaiah
• 0
8
votes
6
replies
445
views
Low Alignment rate
alignment
low_alignment_rate
Bowtie2
updated 3 days ago by
dsull
★ 4.8k • written 5 days ago by
Mehwish
▴ 10
0
votes
6
replies
338
views
Bacterial Pangenome Analysis
Prokka
Pangenome
bacteria
Roary
2 days ago by
kirankumareripogu
▴ 10
0
votes
8
replies
440
views
Violin plot (Monocle 3) - Troubleshooting
Monocle3
updated 3 days ago by
Ram
40k • written 4 days ago by
seattlescientist_01
• 0
1
vote
8
replies
331
views
How to plot proportion of cells in each cluster with scanpy?
scRNA-seq
scanpy
single-cell
3 days ago by
bioinfo
▴ 110
0
votes
6
replies
333
views
Troubles launch IGV on Linux(Debian)
IGV
1 day ago by
PoscaXVIII
• 0
0
votes
4
replies
240
views
Comparing multiple columns from two files using AWK
awk
updated 3 days ago by
Ram
40k • written 3 days ago by
emiliomastriani
▴ 30
0
votes
1
reply
139
views
DESeq2 error - converting result object into dataframe
R
DESeq2
Shiny
updated 3 days ago by
Ram
40k • written 3 days ago by
shasabhi1
• 0
0
votes
1
reply
134
views
Calculating haplotype and nucleotide diversity with deep amplicon sequencing data
haplotype
diversity
Pegas
Arlequin
HTS_data
3 days ago by
deorugz
• 0
3
votes
3
replies
2.2k
views
WGCNA Labeled Heatmap
heatmap
wgcna
updated 3 days ago by
Ram
40k • written 2.0 years ago by
Anand
▴ 40
0
votes
0
replies
102
views
How to remove chimera from 16S Sanger contigs
sanger-sequencing
chimera
uchime
updated 3 days ago by
Ram
40k • written 3 days ago by
eimanpharmacist
▴ 20
3
votes
0
replies
131
views
Herald:
The Biostar Herald for Tuesday, September 26, 2023
herald
3 days ago by
Biostar
2.0k
1
vote
5
replies
219
views
bcftools error merging two VCFs: REF prefixes differ
bcftools
VCF
3 days ago by
Shane
• 0
0
votes
1
reply
311
views
Gene enrichment analysis of prokaryotes gene
prokaryotes
GO
enrichment
DEG
updated 4 days ago by
dthorbur
▴ 550 • written 8 days ago by
Genta
• 0
0
votes
1
reply
145
views
GWAS phenotype
R
GWAS
phenotype
updated 4 days ago by
dthorbur
▴ 550 • written 4 days ago by
sooni
▴ 10
0
votes
2
replies
684
views
RSEM not giving .genes.results and isoforms.results ; Plase check if you provide correct parameters/options for the pipeline!
genomics
transcriptome
rsem
ngs
2 days ago by
kat.bi
• 0
0
votes
2
replies
203
views
how to create a loop in R
R
offtopic
updated 3 days ago by
Ram
40k • written 4 days ago by
mthm
▴ 50
0
votes
4
replies
208
views
BED files
BED
NGS
Chromosomes
updated 3 days ago by
Ram
40k • written 4 days ago by
adarsh_pp
▴ 30
5
votes
7
replies
326
views
How the first sequencing template removed in pair end sequencing
NGS
RNA-seq
WGS
sequence
updated 3 days ago by
GenoMax
134k • written 4 days ago by
octpus616
▴ 90
0
votes
4
replies
261
views
Saving the output of LD pruning from SNPRelate package as a new GDS file
LD-pruning
SNPRelate
updated 3 days ago by
DBScan
▴ 200 • written 4 days ago by
Patrick
• 0
0
votes
2
replies
485
views
Average Coverage after Assembly (Spades)
coverage
average
assembly
updated 4 days ago by
taniapsduarte
• 0 • written 13 months ago by
davidmaimoun
▴ 50
0
votes
5
replies
740
views
Can vg take MUMmer output as input?
vg
updated 3 days ago by
Ram
40k • written 2.8 years ago by
ac2278
▴ 20
2
votes
5
replies
1.9k
views
6 follow
Samtools Failing to Install through Conda
samtools
conda
updated 3 days ago by
Ram
40k • written 17 months ago by
jjp55
▴ 20
3
votes
10
replies
468
views
Should I Learn Docker to Run Command Line Bioinformatics Tool?
Docker
updated 3 days ago by
ATpoint
77k • written 4 days ago by
arriyaz.nstu
▴ 30
0
votes
1
reply
164
views
GO analysis after DESeq2
GO
updated 4 days ago by
Ram
40k • written 4 days ago by
oduduabasi.isaiah
• 0
3
votes
2
replies
194
views
Program for Overlapping DMRs (Differentially Methylated Regions) Between Groups
methylation
DMR
updated 4 days ago by
Basti
★ 1.7k • written 4 days ago by
epianalysis
• 0
0
votes
0
replies
109
views
News:
New NCBI Datasets APIs to Replace Old Ones
NCBI
API
Datasets
4 days ago by
PeterC_NCBI
▴ 330
1
vote
3
replies
243
views
miRDeep2 - identifying miRNAs from deep sequencing data
microRNA
miRDeep2
updated 4 days ago by
Ram
40k • written 4 days ago by
Hasan
• 0
3
votes
4
replies
216
views
Unable to build applet in DNAnexus, .jar file not found
dnanexus
applet
updated 4 days ago by
LChart
3.4k • written 4 days ago by
_quantum_girl_
▴ 10
1
vote
4
replies
273
views
geom_signif() uses t-test to compare between more than 3 groups... Isn't this wrong?
ggplot2
ANOVA
t-test
4 days ago by
RM123
▴ 10
1,000 results • Page
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Comment: ncbi error report log for validate fastq issue
Comment: ncbi error report log for validate fastq issue
A: Bowtie2 MAPQ difference using local vs end-to-end alignment
C: Bowtie2 MAPQ difference using local vs end-to-end alignment
Answer: TPM RNA-seq data for differential expression analysis
A: Error When Installing Emboss
Answer: Subclustering of intergated cells from scRNA-seq data
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Recent Replies
Comment: Getting the overlap between two GTF files
by
GenoMax
134k
Something in `AGAT` should work: https://agat.readthedocs.io/en/latest/?badge=latest
Answer: Automate the Splitting of a VCF File by Sample (bcftools)
by
Pierre Lindenbaum
157k
> My problem has been with trying to loop the process. ok here is a **nextflow** based solution,(NOT tested) workflow { …
Comment: Assessing Rockhopper's output
by
langziv
▴ 50
I see. I tried using the GUI Rockhopper for RNA-seq analysis of a K. pneumoniae strain, but the program didn't find the strain's assembly…
Answer: Getting the overlap between two GTF files
by
Pierre Lindenbaum
157k
bedtools intersect \ -a <(awk '/^[^#]/ {printf("%s\t%d\t%s\t%s\n",$1,int($4)-1,$5,$0);}' file1.gtf | sort -t $'\t' -k1,1 -k2…
Comment: Assessing Rockhopper's output
by
shelkmike
▴ 980
I used Rockhopper only for bacterial genomes assembled into circular contigs or circular scaffolds.
Comment: Unable to install HorvathMammalMethylChip40manifest packages
by
ATpoint
77k
There is no package of that name in CRAN or Bioconductor. Where do you have it from, so which tutorial you follow?
Comment: Subclustering of intergated cells from scRNA-seq data
by
fifty_fifty
▴ 60
thank you. This is exactly what I was looking for
Answer: Search RCSB with a list of protein names?
by
Mensur Dlakic
★ 25k
This is not the most elegant solution, but it should work with previous suggestions. In this remote directory: http://ftp.wwpdb.org/p…
Answer: Automate the Splitting of a VCF File by Sample (bcftools)
by
Ram
40k
The manual says you can split samples out at once using the `+split` plugin. So, `bcftools +split -Oz -o <PATH> file.vcf.gz` should do the …
Comment: differences between trajectories in conditions with Monocle3 or other tools
by
Amitm
★ 2.2k
Phate (or Monocle) doesn't do UMAP. If you have done UMAP already (using seurat) then you could use the cell barcode to UMAP cluster label …
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I figured out how to make it work. The bams have to be comma separated not just by space: cd "${input_directory_with_associated_bam_fi…
Comment: Search RCSB with a list of protein names?
by
Joseph
• 0
Hey, this is so close to what I'm looking for - I clarified in my edit. This is a great way to batch download lots of PDBs in RCSB by their…
Comment: Search RCSB with a list of protein names?
by
Joseph
• 0
This is a really neat package, but I don't think it does exactly what I want, so I've clarified my question. This seems like it grabs a PD…
Comment: How to get the gft file to run velocyto for velocity analysis?
by
Chris
▴ 200
Yes, thank you! I figured it out but I got an error: velocyto run10x -m Hg38_rmsk.gtf WT/outs /cellranger/reference/refdata-cellranger…
Comment: How to get the gft file to run velocyto for velocity analysis?
by
bk11
★ 1.3k
You can get from here- [UCSC][1]. Just click on get output botton. [1]: https://genome.ucsc.edu/cgi-bin/hgTables?hgsid=611454127_NtvlaW…
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