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1,000 results • Page
4 of 20
Sort: Votes
Rank
Views
Votes
Replies
65
votes
3
replies
281k
views
Understanding up and down regulated genes from LOG2 foldchange or foldchange
protein
gene-expression
RNA-Seq
updated 6 months ago by
Ram
40k • written 9.3 years ago by
Whoknows
▴ 960
65
votes
25
replies
14k
views
12 follow
Tool:
sra-explorer : find SRA and FastQ download URLs in a couple of clicks
fastq
SRA
updated 11 weeks ago by
Marquis
• 0 • written 4.6 years ago by
Phil Ewels
★ 1.3k
65
votes
9
replies
48k
views
7 follow
Tutorial:
Reference Assembly - Mapping Reads To A Reference Genome
reference
samtools
mapping
bwa
updated 7 months ago by
Ram
40k • written 10.3 years ago by
Joseph Hughes
★ 3.0k
64
votes
24
replies
2.5k
views
12 follow
Forum:
How much I must feel useless?
machine-learning
Programming
statistics
updated 4 months ago by
Ram
40k • written 5.0 years ago by
Molecular_genetics
▴ 80
64
votes
8
replies
8.0k
views
7 follow
Forum:
Isaac Asimov Accurately Predicts Woes Of Bioinformatician
bioinformatician
updated 7 months ago by
Ram
40k • written 10.4 years ago by
Obi Griffith
20k
64
votes
9
replies
102k
views
10 follow
Blog:
Gene expression units explained: RPM, RPKM, FPKM and TPM
gene-expression
RNA-Seq
updated 7 months ago by
Ram
40k • written 6.0 years ago by
Renesh
★ 2.1k
64
votes
5
replies
7.0k
views
10 follow
Tutorial:
Informatics of RNA-Seq.
next-gen
RNA-Seq
updated 11 months ago by
Ram
40k • written 8.1 years ago by
GouthamAtla
12k
64
votes
26
replies
12k
views
7 follow
Tutorial:
Parallel processing in R
biocparallel
mclapply
foreach
R
updated 6 months ago by
Ram
40k • written 6.0 years ago by
Kevin Blighe
86k
64
votes
29
replies
15k
views
15 follow
Forum:
Fundamentals Of Designing Biological Databases?
database
updated 7 months ago by
Ram
40k • written 12.9 years ago by
anon1234z
▴ 130
64
votes
19
replies
21k
views
12 follow
What Ngs Read Simulators Are Available For Paired-End Data?
next-gen
sequencing
fastq
simulation
paired
updated 10 months ago by
Ram
40k • written 13.1 years ago by
Aaronquinlan
12k
63
votes
28
replies
3.4k
views
11 follow
Forum:
Bioinformatics analyses & errors: need for better post-publication tools?
next-gen
RNA-Seq
updated 5 months ago by
Ram
40k • written 7.4 years ago by
ablanchetcohen
★ 1.2k
63
votes
9
replies
3.7k
views
Forum:
Should The "Post Closing" Option Exist? Vote Now!
meta
updated 7 months ago by
Ram
40k • written 10.3 years ago by
Istvan Albert
98k
63
votes
27
replies
26k
views
18 follow
Data Too Big To Be Stored In Memory: Common Options
python
updated 7 months ago by
Ram
40k • written 10.9 years ago by
Click downvote
▴ 720
63
votes
11
replies
15k
views
9 follow
Forum:
Web Based Interactive Resources To Learn Programming And/Or Bioinformatics
programming
updated 7 months ago by
Ram
40k • written 10.2 years ago by
Malachi Griffith
19k
62
votes
14
replies
189k
views
9 follow
How To Deal With Duplicate Row Names Error In R
r
updated 5 months ago by
zx8754
11k • written 10.6 years ago by
Diana
▴ 900
62
votes
22
replies
60k
views
16 follow
How To Convert Gencode Gtf Into Bed Format ?
gtf
bed
updated 9 months ago by
leshaker
• 0 • written 10.9 years ago by
biorepine
★ 1.5k
62
votes
95
replies
17k
views
8 follow
How to extract the list of genes from TCGA CNV data
TCGA
CNV
genes
updated 7 months ago by
Di
▴ 10 • written 5.4 years ago by
Chaimaa
▴ 260
62
votes
16
replies
33k
views
18 follow
Job:
Bioinformatics Jobs You Can Do From Home?
python
education
updated 7 months ago by
Ram
40k • written 13.0 years ago by
Ivana
▴ 290
62
votes
17
replies
33k
views
7 follow
how to remove multiallelic from VCF
SNP
updated 7 months ago by
Ram
40k • written 8.4 years ago by
xinhui.wang
▴ 520
62
votes
24
replies
40k
views
12 follow
How to extract unique mapped results from Bowtie2 bam results?
alignment
updated 9 months ago by
Ram
40k • written 9.3 years ago by
zhwjmch
▴ 170
61
votes
6
replies
9.2k
views
Tutorial:
Minimum Standards For Bioinformatics Command Line Tools
command-line
updated 7 months ago by
Ram
40k • written 10.1 years ago by
Medhat
9.7k
61
votes
15
replies
13k
views
Tutorial:
Which human reference genome should I use?
assembly
genome
alignment
updated 4 months ago by
Ram
40k • written 5.0 years ago by
finswimmer
16k
61
votes
70
replies
12k
views
21 follow
Forum:
Biostar 2.0 released
meta
biostars
updated 7 months ago by
Ram
40k • written 9.5 years ago by
Istvan Albert
98k
61
votes
29
replies
6.9k
views
14 follow
Best bioinfo one-liners?
linux
updated 8 months ago by
Ram
40k • written 8.4 years ago by
Manu Prestat
4.1k
61
votes
51
replies
21k
views
9 follow
Tool:
Efficiently process (view, analize, clip ends, convert, demultiplex, dereplicate) SFF/FastQ files
sff
sequence
fastq
next-gen
updated 3 months ago by
Ram
40k • written 9.4 years ago by
BioApps
▴ 790
61
votes
14
replies
19k
views
13 follow
Why is Hadoop not used a lot in bio-informatics?
hadoop
updated 6 months ago by
Ram
40k • written 9.0 years ago by
William
★ 5.2k
61
votes
16
replies
7.1k
views
14 follow
Forum:
Rust or C++, what to learn after Go for high-performance bioinformatics tools?
library
updated 8 months ago by
Samuel Lampa
★ 1.3k • written 2.9 years ago by
shenwei356
8.2k
60
votes
2
replies
7.1k
views
Tutorial:
Table Of Contents To All Review Paper Compilations On Biostar
papers
updated 7 months ago by
Ram
40k • written 11.1 years ago by
Istvan Albert
98k
60
votes
18
replies
3.5k
views
10 follow
Are Super User Style Questions Appropriate In Biostar?
meta
biostars
updated 7 months ago by
Ram
40k • written 13.2 years ago by
Andrewjgrimm
▴ 460
60
votes
21
replies
2.9k
views
14 follow
Reproduce the article "The complete sequence of a human genome."
genome
assembly
updated 25 days ago by
Michael
53k • written 26 days ago by
sqshigg
▴ 60
60
votes
52
replies
41k
views
23 follow
Tutorial:
Creating Interactive Volcano Plots with R and Plot.ly
plotly
R
volcano-plot
updated 6 months ago by
Ram
40k • written 7.0 years ago by
steve
★ 3.3k
60
votes
28
replies
7.6k
views
27 follow
Forum:
Jim Robinson of the Integrative Genomics Viewer (IGV) uses this
uses-this
updated 6 months ago by
Ram
40k • written 9.0 years ago by
Istvan Albert
98k
60
votes
3
replies
32k
views
Paired-end reads - what is what?
RNA-Seq
updated 8 months ago by
Ram
40k • written 8.3 years ago by
darxsys
▴ 220
60
votes
12
replies
38k
views
10 follow
Get chromosome sizes from fasta file
genome
ucsc
updated 10 months ago by
jamesogilvie1
• 0 • written 7.7 years ago by
rioualen
▴ 710
60
votes
17
replies
32k
views
16 follow
Scientific Names In Blast Output And Databases
blast
blast+
updated 9 months ago by
Ram
40k • written 10.2 years ago by
Carlos Borroto
★ 2.1k
60
votes
14
replies
11k
views
16 follow
Forum:
Interesting bioinformatics blogs (2017 edition)
blogs
updated 7 months ago by
Ram
40k • written 6.5 years ago by
WouterDeCoster
47k
59
votes
43
replies
19k
views
18 follow
Tutorial:
Polish PacBio assembly with latest PacBio tools : an affordable solution for everyone
pbalign
pacbio
arrow
smrt-limk
updated 6 months ago by
Ram
40k • written 6.0 years ago by
Rox
★ 1.4k
59
votes
31
replies
4.1k
views
12 follow
News:
Example of how bioinformaticians can publish in Scientific Reports (by nature publishing group) using publicly available NGS data
publication
NGS
updated 5 months ago by
Ram
40k • written 6.7 years ago by
David Langenberger
10k
58
votes
25
replies
38k
views
15 follow
Tutorial:
Protocol To Downlad TCGA Data From GDC
GDC
methylation
TCGA
updated 6 months ago by
Ram
40k • written 7.2 years ago by
Shicheng Guo
★ 9.3k
57
votes
15
replies
5.6k
views
9 follow
Forum:
Good practices while dealing with very large datasets
Big-Data
Programming
updated 4 months ago by
Ram
40k • written 6.4 years ago by
VHahaut
★ 1.1k
57
votes
19
replies
27k
views
6 follow
Forum:
Attention: Bowtie2 And Multiple Hits
next-gen
bowtie
mapping
updated 7 months ago by
Ram
40k • written 10.9 years ago by
David Langenberger
10k
57
votes
9
replies
5.4k
views
14 follow
Forum:
Handbooks for Bioinformatics Ninjas
gallery
updated 5 months ago by
Ram
40k • written 7.4 years ago by
Shicheng Guo
★ 9.3k
57
votes
15
replies
4.4k
views
9 follow
Forum:
Entice Biologists To Learn R
r
updated 7 months ago by
Ram
40k • written 12.0 years ago by
Madelaine Gogol
5.3k
57
votes
43
replies
15k
views
8 follow
Forum:
Best RNA-Seq aligner: A comparison of mapping tools
NGS
alignment
genome
RNA-Seq
sequence
updated 6 months ago by
Ram
40k • written 4.7 years ago by
David Langenberger
10k
56
votes
22
replies
23k
views
7 follow
Batch correction in DESeq2
DESeq2
Batch Effect
Combat
updated 6 months ago by
Kevin Blighe
86k • written 4.0 years ago by
Arindam Ghosh
▴ 510
56
votes
23
replies
4.5k
views
10 follow
Tool:
ASCIIGenome: Text Only Genome Viewer!
terminal
genome-browser
java
ASCIIGenome
updated 3 months ago by
Ram
40k • written 7.2 years ago by
dariober
14k
56
votes
7
replies
7.5k
views
8 follow
What is the best way to combine machine learning algorithms for feature selection such as Variable importance in Random Forest with differential expr…
RNA-Seq
rna-seq
updated 6 weeks ago by
Kevin Blighe
86k • written 5.5 years ago by
elmahy2005
▴ 150
56
votes
19
replies
3.7k
views
9 follow
Forum:
How to apply for a job
resume
cover-letter
application
CV
updated 4 months ago by
Ram
40k • written 6.2 years ago by
Emily
23k
56
votes
25
replies
3.7k
views
8 follow
Forum:
Am I crazy, or are most published RNA-seq studies vastly underpowered?
sample-size
statistical-power
RNA-Seq
updated 3 months ago by
Ram
40k • written 3.1 years ago by
telroyjatter
▴ 210
56
votes
4
replies
66k
views
What is difference between soft-clipped and hard-clipped in SAM specification?
sequence
next-gen-sequencing
updated 11 months ago by
Ram
40k • written 8.9 years ago by
mangfu100
▴ 800
1,000 results • Page
4 of 20
Recent Votes
How to assign read groups to bam files?
C: sra-explorer : find SRA and FastQ download URLs in a couple of clicks
C: sra-explorer : find SRA and FastQ download URLs in a couple of clicks
Answer: Which function is best for pathway analysis?
Comment: Hisat2 index and alignment question
Answer: Low Alignment rate
Answer: Unable to build applet in DNAnexus, .jar file not found
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Answer: Fetch Fastq files directly for SRA data
by
Rob
6.2k
What is the accession for the project? Many — though not all — of the projects present in the NCBI SRA are also hosted in the European Nuc…
Comment: Dataset's name in BioMart for S. pombe
by
YUYANG.OON
• 0
Works with `useMart(biomart="fungi_mart", dataset="spombe_eg_gene", host="https://fungi.ensembl.org")`
Comment: Bug of vg surject
by
Jordan M Eizenga
▴ 360
This is still hard to interpret without the commands. What would help is if you literally copied the full UNIX commands along with their re…
Comment: Which function is best for pathway analysis?
by
Chris
▴ 180
Thank you for your reply! Do you have code that I and others can implement? I am not sure how to customize the gmt file from my differentia…
Comment: Bug of vg surject
by
Qi
• 0
Thanks your reply! my resulte show below: 5x_pan_sv.bam 1347587601 #converted from gam to bam F1 indel=0.830392 snp=0.932511 5…
Comment: Bug of vg surject
by
Jordan M Eizenga
▴ 360
It might help us to diagnose the problem if you included the exact commands that you used and showed the results that you are concerned abo…
Answer: Which function is best for pathway analysis?
by
Yogi
▴ 20
Few things to mention. Pathway Enrichment Analysis can be split into two categories. 1. Functional Class Scoring Methods 2. Overrepr…
Answer: identify DEGs across all conditions and per specific conditions
by
Yogi
▴ 20
Couple of things. (Feel free to correct if I'm misunderstanding). "DESeq2" is meant for 1v1 comparisons. If you're trying to compar…
Comment: Hisat2 index and alignment question
by
Istvan Albert
98k
yes correct you have to pass the full prefix and not the directory
Comment: Low Alignment rate
by
dsull
★ 4.7k
Wow! Didn't realize the repeat-masked file could have such a large influence on alignment. Very good to know -- I guess I'll need to dive i…
Comment: Troubles launch IGV on Linux(Debian)
by
morovatunc
▴ 550
Disclaimer: My comment could be totally unrelated. I would give these a try if you have noone comment/answer your problem. I have had this…
Comment: How to check RNAseq support for annotated genes?
by
Michael
53k
> I tried it using STAR to map the transcript to the annotation gtf file That is not what STAR is for. STAR is for aligning short reads in…
Comment: Low Alignment rate
by
Mehwish
• 0
Thanks alot! This worked out for me https://ftp.ensembl.org/pub/release-110/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.toplevel.fa.gz …
Comment: Violin plot (Monocle 3) - Troubleshooting
by
Ram
40k
The error message tells me there is the possibility of a conflicting function called `plot_genes_violin` that accepts one parameter and err…
Comment: Violin plot (Monocle 3) - Troubleshooting
by
bk11
★ 1.2k
I m not sure why you have problem there. I do not encounter any problem. May be your gene list are not present in your source object. Pleas…
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