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1,000 results • Page
6 of 20
Sort: Votes
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Views
Votes
Replies
72
votes
15
replies
20k
views
12 follow
Counting N'S Within Fasta
fasta
counts
perl
updated 5.9 years ago by
Biostar
20 • written 12.0 years ago by
PoGibas
5.1k
72
votes
12
replies
4.2k
views
7 follow
Does Pre-Publishing Open Source Algorithms Hurt Chances Of Getting Published?
publication
algorithm
updated 20 months ago by
Ram
43k • written 12.6 years ago by
Martijn Van Iersel
▴ 570
72
votes
28
replies
6.2k
views
10 follow
Forum:
Bioinformatics commercial services as a risk factor for scientific misconduct
commercial-services
ethics
misconduct
updated 21 months ago by
Ram
43k • written 9.2 years ago by
Angle45
▴ 90
72
votes
48
replies
8.3k
views
18 follow
What Kind Of Bioinformatics Tutorials Would You Like To See Online?
education
updated 13 months ago by
Ram
43k • written 13.0 years ago by
User 59
13k
72
votes
71
replies
30k
views
17 follow
Tool:
EnhancedVolcano: Publication-ready volcano plots with enhanced colouring and labeling
volcano-plot
bioconductor
ggplot2
ggrepel
updated 9 months ago by
Ram
43k • written 5.6 years ago by
Kevin Blighe
87k
72
votes
18
replies
55k
views
13 follow
Extracting Multiple Fasta Sequences At A Time From A File Containing Many Sequences
fasta
sequence
parsing
perl
updated 13.5 years ago by
Peter
6.0k • written 13.5 years ago by
Sarah
▴ 110
72
votes
20
replies
87k
views
15 follow
How To Get Ensembl Id (Gene, Transcript, Protein) Mapping Information?
ensembl
identifiers
mapping
updated 14 months ago by
Ram
43k • written 13.4 years ago by
Unode
▴ 180
71
votes
16
replies
90k
views
12 follow
What Does Samtools Flagstat Results Mean?
rna
samtools
mouse
updated 2.0 years ago by
Pierre Lindenbaum
160k • written 12.5 years ago by
Sakti
▴ 510
71
votes
15
replies
48k
views
10 follow
Definition of minor and major allele and connection with risk, effect, wildtype and reference allele
risk
minor
major
reference
alleles
written 5.9 years ago by
m98
▴ 420
70
votes
19
replies
5.5k
views
13 follow
Forum:
Thrown Into A Bioinformatics Position
computational-analysis
updated 20 months ago by
Ram
43k • written 8.5 years ago by
System
▴ 170
70
votes
15
replies
16k
views
12 follow
Transcription Factor Enrichment
transcription
transcript
sequence
enrichment
updated 7.4 years ago by
jin
▴ 80 • written 13.5 years ago by
Dave Bridges
★ 1.4k
70
votes
33
replies
17k
views
18 follow
Choosing Random Set Of Seqs From Larger Set
random
fasta
updated 23 months ago by
Ram
43k • written 12.0 years ago by
razor
▴ 190
70
votes
5
replies
11k
views
8 follow
Tutorial:
Bioinformatics For Metagenomic Analyses
QC
taxonomy
metagenomics
updated 24 months ago by
Ram
43k • written 10.0 years ago by
umer.zeeshan.ijaz
★ 1.8k
70
votes
29
replies
15k
views
13 follow
Tutorial:
Generating consensus sequence from bam file
fasta
consensus
bam
updated 15 days ago by
jkbonfield
★ 1.2k • written 5.1 years ago by
finswimmer
16k
70
votes
16
replies
9.9k
views
11 follow
Creating A New Bioinformatics Unit
core
career
updated 13 months ago by
Ram
43k • written 12.8 years ago by
Israel Barrantes
▴ 790
69
votes
25
replies
6.9k
views
16 follow
How Do You Explain What You Do To The Guy On The Street Or Your Mum?
general
updated 4 months ago by
Ram
43k • written 14.0 years ago by
Dave Gerrard
▴ 190
69
votes
17
replies
38k
views
12 follow
Transform a GTF file into a data frame in R
R
RNA-Seq
GTF
updated 13 months ago by
Ram
43k • written 6.5 years ago by
biomagician
▴ 410
69
votes
22
replies
39k
views
17 follow
Plotting Density Of Snps On Chromosomes
snp
snp
plot
updated 6.7 years ago by
kaixian110
• 0 • written 12.0 years ago by
Leszek
4.2k
69
votes
39
replies
4.5k
views
14 follow
Forum:
Preparation for Bioinformatics.SE closing
meta
biostars
updated 10 months ago by
Ram
43k • written 6.8 years ago by
John
13k
69
votes
13
replies
37k
views
8 follow
How Do People Go About Pubmed Text Mining
pubmed
text
updated 12.7 years ago by
Chris Evelo
10k • written 12.7 years ago by
Lyco
★ 2.3k
68
votes
22
replies
7.7k
views
16 follow
Forum:
Learning Python: list of resources
python
updated 11 months ago by
Ram
43k • written 7.4 years ago by
dago
★ 2.8k
68
votes
12
replies
30k
views
8 follow
Drug to Target/Gene/Protein Interaction Databases
protein
target
database
drug
gene
updated 2.6 years ago by
Ram
43k • written 12.1 years ago by
Wayne
★ 1.0k
68
votes
5
replies
22k
views
8 follow
Can someone please explain in simple terms how DESeq2 works?
DESeq2
updated 24 months ago by
Ram
43k • written 9.2 years ago by
BioStars1993
▴ 130
68
votes
18
replies
128k
views
14 follow
from .BAM to .BAI using samtools
next-gen-sequencing
samtools
updated 8 weeks ago by
Pierre Lindenbaum
160k • written 9.5 years ago by
Kizuna
▴ 870
68
votes
23
replies
36k
views
16 follow
How Do I Do Simple Go Term Lookup Given A Gene (Or Mrna) Identifier?
gene
annotation
database
updated 3.0 years ago by
Ram
43k • written 13.8 years ago by
Ryan Thompson
★ 3.6k
68
votes
40
replies
6.2k
views
11 follow
Can We Agree On A Short Twitter Hashtag For Nextgen Sequencing?
next-gen
sequencing
sequencing
updated 12.4 years ago by
pmenzel
▴ 310 • written 12.5 years ago by
Samuel Lampa
★ 1.3k
68
votes
48
replies
8.6k
views
16 follow
Forum:
Stranger Things: unexpected limitations of popular tools
samtools
hisat2
updated 10 months ago by
Ram
43k • written 6.7 years ago by
Istvan Albert
100k
67
votes
9
replies
12k
views
Rna-Seq: Novel Transcripts Found. What Next?
rna-seq
updated 10.8 years ago by
Devon Ryan
104k • written 10.8 years ago by
jobinv
★ 1.1k
67
votes
18
replies
73k
views
17 follow
Project For A Beginner Bioinformatics Student
project
java
updated 20 months ago by
Ram
43k • written 12.4 years ago by
And
▴ 230
67
votes
25
replies
15k
views
12 follow
Tool:
sra-explorer : find SRA and FastQ download URLs in a couple of clicks
fastq
SRA
updated 5 months ago by
GenoMax
140k • written 5.1 years ago by
Phil Ewels
★ 1.4k
67
votes
40
replies
9.6k
views
12 follow
Forum:
I want to re-open the old debate: python or perl ?
perl
python
updated 2.1 years ago by
Ram
43k • written 9.4 years ago by
Gabriel R.
★ 2.9k
67
votes
17
replies
23k
views
15 follow
What Are The 'Copy Number Detection' Tools Out There For Exome Capture Ngs Data.
cnv
copynumber
next-gen
sequencing
variant
structural
updated 2.1 years ago by
Ram
43k • written 12.4 years ago by
Prateek
★ 1.0k
67
votes
21
replies
6.0k
views
8 follow
Forum:
Meta: Markdown in Biostars (now enabled)
meta
12 months ago by
Ram
43k
67
votes
13
replies
12k
views
12 follow
Good Books And Resources For Figuring Out Biostatistics?
statistics
books
updated 2.8 years ago by
Ram
43k • written 12.8 years ago by
Adamc
▴ 680
67
votes
6
replies
26k
views
7 follow
Tutorial:
Rna-Seq Review Papers
RNA-seq
papers
updated 13 months ago by
Ram
43k • written 11.6 years ago by
Istvan Albert
100k
67
votes
15
replies
33k
views
14 follow
Rna-Seq Pipeline
pipeline
next-gen-sequencing
rna
rna-seq
updated 10 months ago by
Ram
43k • written 13.9 years ago by
brentp
24k
67
votes
6
replies
28k
views
8 follow
What Is A "Spot" In Sra Format
sra
fastq
format
updated 14 months ago by
Ram
43k • written 12.5 years ago by
Daniel Standage
4.1k
66
votes
19
replies
50k
views
17 follow
Tool To Find Out If Fastq Is In Sanger Or Phred64 Encoding?
fastq
updated 9 months ago by
Ram
43k • written 11.1 years ago by
14134125465346445
★ 3.6k
66
votes
25
replies
6.5k
views
13 follow
Data Selection With/Without Databases (Large Data Sets, Orms, And Speed)
database
python
data
updated 11.1 years ago by
kwete90
• 0 • written 13.0 years ago by
Sequencegeek
▴ 740
66
votes
23
replies
48k
views
8 follow
extract dendrogram cluster from pheatmap
R
updated 12 days ago by
Getnet Tesfaw
• 0 • written 6.3 years ago by
1769mkc
★ 1.2k
66
votes
9
replies
109k
views
10 follow
Blog:
Gene expression units explained: RPM, RPKM, FPKM and TPM
gene-expression
RNA-Seq
updated 13 months ago by
Ram
43k • written 6.5 years ago by
Renesh
★ 2.2k
66
votes
29
replies
16k
views
15 follow
Forum:
Fundamentals Of Designing Biological Databases?
database
updated 13 months ago by
Ram
43k • written 13.4 years ago by
anon1234z
▴ 130
66
votes
39
replies
7.3k
views
13 follow
What Do You Consider The Most Trivial And The Most Challenging Tasks In Your Particular Field Of Work?
career
subjective
updated 3.0 years ago by
Ram
43k • written 14.1 years ago by
Marcos De Carvalho
▴ 310
66
votes
43
replies
27k
views
23 follow
Ngs - Huge (Fastq) File Parsing - Which Language For Good Efficiency ?
next-gen
sequencing
parsing
fastq
programming
updated 2.2 years ago by
Ram
43k • written 13.2 years ago by
toni
★ 2.2k
66
votes
21
replies
6.7k
views
18 follow
Forum:
Is there anyone who was a pure biologist?
next-gen
updated 12 months ago by
Ram
43k • written 9.9 years ago by
Chong Tang
▴ 100
66
votes
22
replies
24k
views
9 follow
Finding Gene Symbol Synonyms
annotation
updated 2.1 years ago by
Ram
43k • written 13.8 years ago by
Mike Dewar
★ 1.6k
66
votes
23
replies
10k
views
9 follow
Using Hdf5 To Store Bio-Data
hdf5
storage
database
updated 5 months ago by
Ram
43k • written 14.1 years ago by
Pierre Lindenbaum
160k
66
votes
9
replies
49k
views
7 follow
Tutorial:
Reference Assembly - Mapping Reads To A Reference Genome
reference
samtools
mapping
bwa
updated 13 months ago by
Ram
43k • written 10.8 years ago by
Joseph Hughes
★ 3.0k
66
votes
23
replies
11k
views
13 follow
How To Get Started In Bioinformatics?
books
updated 7.3 years ago by
jp
▴ 120 • written 14.0 years ago by
Oli
▴ 180
66
votes
19
replies
23k
views
13 follow
Online Bioinformatics Training Options
training
updated 11.2 years ago by
parvan.zahra
• 0 • written 12.1 years ago by
John
★ 1.5k
1,000 results • Page
6 of 20
Recent Votes
Announcement: Research Topic "Mechanistic, Machine Learning and Hybrid Models of the 'Other' Endocrine Regulatory Systems in Health and Disease", Volume II
Comment: Number of CPUs to use by DRAP for transcriptomic assmbly
Comment: Gene reads all zero for each sample
Synthetic lethality and cancer database
Answer: log2(TPM+1)
Comment: Error in openning FastQC
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
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Comment: Mapping ortholog human and mouse transcript
by
1769mkc
★ 1.2k
for hgnc and ensembl that can be done for transcript id for human to mouse ortholog its difficult to parse and map the exact version from g…
Comment: Announcement: Research Topic "Mechanistic, Machine Learning and Hybrid Models of
by
Mensur Dlakic
★ 26k
Take it from someone who has hosted both a successful and a failed research topic: these deadlines are unrealistic. I am guessing you are p…
Answer: Tissue specific mRNA expression dataset in human and mouse
by
BioinfGuru
★ 1.6k
Awesome, years ago I wrote my thesis on the use of Tau to calculate tissue specificity, enjoy! The supplementary materials in the paper is…
Answer: Mapping ortholog human and mouse transcript
by
BioinfGuru
★ 1.6k
That error is on the server side, nothing to do with you, and is very common. Usually the fix is to try again in an hour, or to insert host…
Comment: Nanopore multisample variant calling
by
emilydolivo97
• 0
hello @colindaven , Did you find a suitable tool for this purpose please ? I'm dealing with fungal dataset
Comment: How do I get the gene annotations as a text file from a genebank file?
by
BioinfGuru
★ 1.6k
Can you upload or post a link to the genbank file please?
Comment: Mapping ortholog human and mouse transcript
by
manaswwm
▴ 490
I usually perform biomaRt queries with their `getBM()` function where I list the input using `filter` and `values` option and my request in…
Comment: DESeq2 and normalization
by
JH
• 0
Thank you for your response. I have often seen commands like dds <- estimateSizeFactors(dds) normalized_counts <- counts(dds, normalized=T…
Comment: Number of CPUs to use by DRAP for transcriptomic assmbly
by
Mohamed Abderrahmane
▴ 10
It's the case that there's no indication of multi-threading in the documentation. However, it made me wonder why the developers would restr…
Answer: Is RNAseq data from a monoclonal cell line technically considered scRNAseq?
by
Soheil
▴ 90
Bulk RNAseq from any sample is still considered a bulk sample. Running bulk RNA sequencing on a sample with any level of cell type/state c…
Comment: Is it reasonable to discard reads that show variation of quality across its leng
by
BRENO
• 0
Yikes. Well, this is from an Illumina MiniSeq. Sure, I will use alignment, but only to remove host reads. Then the remaining reads will go …
Comment: Is it reasonable to discard reads that show variation of quality across its leng
by
GenoMax
140k
This is going to be tough to diagnose. Are you going to align to a reference? If so go ahead with the alignments to see if alignments turn …
Comment: Is it reasonable to discard reads that show variation of quality across its leng
by
BRENO
• 0
Thank you for replying. I have separated the reads with at least one N on them and they account for 0.2% of the total reads in the file (8'…
Comment: Error running local blastn in R using system2
by
Mensur Dlakic
★ 26k
The `-db data/nt_euk` switch means that in your current directory you have a subdirectory called `data`, and all the `nt_euk` files are in …
Comment: Low number of both surviving reads after trimming
by
Jay
• 0
Thank you for your opinion. I will try with lower LEADING and TRAILING bases. By the way, I tried to set `keepBothReads:true` through sear…
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