Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : all time
all time
today
this week
this month
this year
1,000 results • Page
1 of 20
Sort: Views
Rank
Views
Votes
Replies
23
votes
22
replies
38k
views
6 follow
Converting VCF to PLINK .bed binary fileset to check for pedigree errors with KING: How do conversion tools make the PLINK .fam file, without asking …
plink
vcf
fam
gatk
vcftools
updated 3 months ago by
Nicole
• 0 • written 4.9 years ago by
gaelgarcia
▴ 250
4
votes
4
replies
33k
views
How to convert a URL into a DOI programmatically
pubmed
doi
url
written 6.5 years ago by
entheologist33
▴ 100
20
votes
15
replies
25k
views
8 follow
Converting SNP from chr:pos to rs number using PLINK?
plink
SNP
updated 8 months ago by
Ram
38k • written 7.2 years ago by
dam4l
▴ 190
4
votes
13
replies
25k
views
6 follow
[main_samview] fail to read the header from sample.bam
alignment
updated 8 months ago by
Qboy
• 0 • written 5.1 years ago by
sambioinfo2018
▴ 20
4
votes
6
replies
23k
views
terminate called after throwing an instance of 'std::bad_alloc?
bedtools
linux
data
3.9 years ago by
star
▴ 330
1
vote
3
replies
23k
views
How to extract vcf.gz and vcf.gz.tbi
SNP
sequence
gene
updated 4.7 years ago by
Pierre Lindenbaum
153k • written 4.7 years ago by
elhlalisoufiane
▴ 20
3
votes
4
replies
21k
views
Using nr database for BLAST search
nr
database
blast
blastp
updated 6.6 years ago by
Biostar
20 • written 6.7 years ago by
cookm346
▴ 20
9
votes
4
replies
20k
views
What is the definition of "read depth" vs "coverage"? (again...)
coverage
depth
read depth
5.9 years ago by
ariel.balter
▴ 190
2
votes
4
replies
20k
views
Error in file(file, "rt") : cannot open the connection
R
MicroRNA TXT FORMAT
updated 7.0 years ago by
GenoMax
127k • written 7.0 years ago by
31sharmajittu1991
• 0
1
vote
5
replies
17k
views
Calculating the sequence similarity and percentage of matched sequences
alignment
sequence
updated 5.3 years ago by
eteleeb
• 0 • written 5.4 years ago by
ago1mutant
▴ 10
7
votes
16
replies
17k
views
featureCounts: Low percentage of assigned fragments
RNA-Seq
rna-seq
6.7 years ago by
aggregatibacter
▴ 170
27
votes
17
replies
16k
views
12 follow
Dotplot for filtered pathways result
pathways
ggplot2
clusterProfiler
r
dotplot
15 months ago by
Leite
★ 1.2k
2
votes
8
replies
16k
views
Using vcfutils.pl command
alignment
updated 11 months ago by
Ram
38k • written 8.1 years ago by
lcc1844
▴ 40
2
votes
10
replies
16k
views
How do you use the Aspera ascp command?
aspera
gigascience
gigaDb
updated 18 months ago by
Ram
38k • written 7.5 years ago by
cyril-cros
▴ 940
16
votes
38
replies
15k
views
Differential gene Analysis by Limma
Limma
Microarray
R
updated 11 months ago by
Ram
38k • written 8.1 years ago by
Mo
▴ 920
2
votes
13
replies
15k
views
convert text file to fasta file
blast
updated 6.6 years ago by
GouthamAtla
12k • written 6.6 years ago by
sukesh1411
▴ 30
6
votes
2
replies
15k
views
Pacbio Ccs Vs Subreads Explained ?
updated 8.9 years ago by
Biostar
20 • written 9.4 years ago by
curious.genome
▴ 40
0
votes
8
replies
15k
views
find ORF in sequence python
sequence
updated 6.3 years ago by
natasha.sernova
★ 3.9k • written 6.3 years ago by
elisheva
▴ 120
2
votes
2
replies
15k
views
How to calculate the insert size for Paired-end reads
SSPACE
insert-size
6.7 years ago by
Ric
▴ 430
2
votes
3
replies
15k
views
Subsetting - Raw Counts from Seurat Object
seurat
raw counts
RNA-Seq
2.9 years ago by
David_emir
▴ 460
4
votes
10
replies
14k
views
How to plot a multiple line graph with Mean and Std Error for following dataset?
R
updated 4.9 years ago by
GenoMax
127k • written 4.9 years ago by
WUSCHEL
▴ 720
0
votes
3
replies
14k
views
How to choose a TPM cut-off point
RNA-Seq
transcript
tpm
updated 7 months ago by
Ram
38k • written 7.4 years ago by
amyfm
▴ 10
16
votes
14
replies
14k
views
Which truseq trimmomatic adapters file to use when removing truseq adapters?
adapters
trimmomatic
RNA-seq
4.8 years ago by
salamandra
▴ 530
5
votes
4
replies
14k
views
glmnet package lasso error
glmnet
lasso
multilinear regression
5.1 years ago by
demoraesdiogo2017
▴ 90
0
votes
3
replies
14k
views
Error in (function (classes, fdef, mtable): unable to find an inherited method for function ‘annotation’ for signature ‘"character
inSilicoMerging
8.8 years ago by
ira_grich
• 0
2
votes
4
replies
14k
views
TRIMMOMATRIC Nextera trimming
next-gen
updated 5 months ago by
Ram
38k • written 7.7 years ago by
meekadi
▴ 10
3
votes
1
reply
13k
views
How do you normalize Transcript per Million TPM to compare between samples ?
RNA-Seq
TPM
STAR
salmon
quantification
6.2 years ago by
ZheFrench
▴ 500
1
vote
8
replies
13k
views
HTSeq-count high number of no_feature
htseq-count
rna-seq
updated 11 months ago by
Ram
38k • written 8.1 years ago by
cafelumiere12
▴ 80
8
votes
5
replies
13k
views
The comparison between HISAT2 and Tophat2
RNA-Seq
Tophat2
HISAT2
updated 4.6 years ago by
Ram
38k • written 7.1 years ago by
Peng Huang
▴ 50
9
votes
13
replies
13k
views
GC Content of Fasta file --- Python Help
sequence
6.1 years ago by
Patrick Brennan
▴ 50
7
votes
4
replies
13k
views
Use of if-else statement in snakemake rule
snakemake
if-else
python
updated 3.4 years ago by
schroder.julia
• 0 • written 5.7 years ago by
Jokhe
▴ 130
0
votes
2
replies
13k
views
Error in -delete: invalid argument to unary operator
RNA-Seq
R
3.1 years ago by
ovariohisterectomia
▴ 40
1
vote
5
replies
12k
views
pheatmap: how to use legend information as row annotation?
R
pheatmap
legend
annotation
5.0 years ago by
n85825
• 0
3
votes
8
replies
12k
views
Error when running pheatmap
pheatmap
Error in hclust
4.0 years ago by
luzglongoria
▴ 40
6
votes
13
replies
12k
views
Fastq file is truncated error message
RNA-Seq
error
fastq
6.8 years ago by
rob.costa1234
▴ 310
0
votes
2
replies
12k
views
How to download pfam database
sequence
updated 6.2 years ago by
Medhat
9.5k • written 6.2 years ago by
Sanchita Gupta
▴ 30
6
votes
13
replies
12k
views
Showing FDR and log2FC in Volcano plot using R
R
RNA-Seq
volcano plot
updated 3.8 years ago by
zx8754
11k • written 5.3 years ago by
Farbod
★ 3.4k
0
votes
4
replies
12k
views
Unable to install XLconnect package in R studio version 3.2.1
R
updated 5 months ago by
Ram
38k • written 7.6 years ago by
Debbie
▴ 10
4
votes
1
reply
12k
views
Converting BigBed to Bed format
Installing BigFile Support
7.8 years ago by
sumaira.zaman10
▴ 20
9
votes
13
replies
12k
views
How to interpret Per sequence GC content module in FastQC for RNA-seq data?
RNA-Seq
FastQC
QC
GC-content
3.6 years ago by
Arindam Ghosh
▴ 470
3
votes
11
replies
12k
views
What is the acceptable % of reads that map to no feature (in RNASeq)?
htseq
RNA-Seq
STAR
updated 11 months ago by
Ram
38k • written 8.2 years ago by
noushin.farnoud
▴ 120
2
votes
4
replies
12k
views
Run BLAST on Linux terminal
ncbi
nBLAST
linux
blast
updated 6 months ago by
Ram
38k • written 7.5 years ago by
Abdul Rafay Khan
★ 1.2k
9
votes
13
replies
12k
views
Sort bam file by coordinates using samtools
sequencing
next-gen
gene
rna-seq
Tool
updated 4.5 years ago by
finswimmer
16k • written 4.5 years ago by
Shahzad
▴ 30
4
votes
18
replies
12k
views
Alignment with BWA and SAMtools
BWA
SAM-file
SAMtools
updated 2.4 years ago by
Biostar
20 • written 4.6 years ago by
Shelle
▴ 30
1
vote
2
replies
12k
views
kart file in prefetch
rna-seq
updated 6.4 years ago by
Biostar
20 • written 6.6 years ago by
zh.khodadadi
▴ 20
0
votes
1
reply
11k
views
PLINK 1.9 - Converting PED/MAP to BED/BIM/FAM with missing values
plink
updated 6.7 years ago by
Biostar
20 • written 6.7 years ago by
rednalf
▴ 80
0
votes
3
replies
11k
views
Extracting Specific Columns from Multiple Files & Writing to File Python
python
file-handeling
6.9 years ago by
BioICoder
▴ 40
0
votes
2
replies
11k
views
GSEA error - After pruning, none of the gene sets passed size thresholds
RNA-Seq
GSEA
gene
genome
updated 3.7 years ago by
Morris_Chair
▴ 330 • written 3.7 years ago by
limjustinj
• 0
4
votes
12
replies
11k
views
STAR alignment error- Segmentation fault (core dumped)
alignment
software error
RNA-Seq
STAR
4.7 years ago by
EagleEye
7.4k
3
votes
2
replies
11k
views
ExAC PLI score calculation
DNA-Seq
6.1 years ago by
jonessara770
▴ 240
1,000 results • Page
1 of 20
Recent Votes
Answer: How can I use bcftools mpileup or an alternative to find ALL variants without an
Answer: What is the NCBI's definition of an "atypical genome"?
Comment: GO enrichment analysis
Comment: GO enrichment analysis
Answer: What is the NCBI's definition of an "atypical genome"?
A: Why is RNA-Seq or cDNA used to detect gene fusions instead of gDNA?
Answer: Somatic truth set
Recent Locations •
All
Boston,
8 minutes ago
United States,
10 minutes ago
South Korea,
12 minutes ago
Brazil,
13 minutes ago
Australia,
15 minutes ago
University of Utah,
18 minutes ago
Canada,
23 minutes ago
Recent Awards •
All
Popular Question
to
pavelasquezv
▴ 50
Popular Question
to
Anand Rao
▴ 550
Scholar
to
andres.firrincieli
3.2k
Scholar
to
Jesse
• 0
Popular Question
to
chaco001
▴ 10
Scholar
to
Jaykumar
▴ 50
Scholar
to
GenoMax
127k
Recent Replies
Answer: Align miRNA library (small RNA-seq) without trimming
by
Ming Tommy Tang
★ 2.9k
bowtie 1 is good for short read < 50bp, how long is your read? bowtie2 is better for reads > 50 bp
Comment: Differences in RNAseq Variant Calling and Allele Specific Expression
by
afei
• 0
Hi! I'm also confused by this. And the site (https://gatkforums.broadinstitute.org/gatk/categories/gatk-support-forum) is closed. Have you …
Comment: Variant caller reports a homozygous variant genotype, but more reads are associa
by
rebeliscu
▴ 50
Indeed, I thought the quality might be a factor. Still, given the information, it seems odd to me that that call was made. Thanks for you…
Answer: How can I use bcftools mpileup or an alternative to find ALL variants without an
by
cfos4698
▴ 730
To generate a VCF file one would normally pipe the input of an `mpileup` command into an actual `call` command. For example: ``` bcftools …
Answer: PTM-aware protein folding / docking
by
Mensur Dlakic
★ 23k
Your assumption is correct - AlphaFold pTM has nothing to do with post-translational modifications. See [**here**][1]. Not to discourage…
Answer: Admixture cv error
by
Declan
• 0
I had this issue today and was able to resolve it by changing the random seed Admixture uses by adding `-s time` (which sets the random see…
Answer: Total No of Genes of GENCODE Release 43
by
ahmad
• 0
The following is the correct code gtf_43<-rtracklayer::import('gencode.v43.primary_assembly.annotation.gtf') dtgtf_43<-data.frame(gtf_4…
Comment: Total No of Genes of GENCODE Release 43
by
ahmad
• 0
Hello, thanks for replying, by mistake I paste the wrong code. the following is the corrected gtf_43<-rtracklayer::import('gencode.v43…
Comment: Kallisto bustools for scRNA-seq
by
dsull
★ 4.0k
I have no idea what that is besides a bunch of jumbled sequences. You'll need look up the structure of the reads / library from wherever yo…
Comment: Aberrant splicing in bulk RNAseq
by
dsull
★ 4.0k
First thing: kallisto cannot interface with UMI-tools (kallisto doesn't use read headers and therefore you're stuck with "the kallisto way"…
Comment: Using Copy Number Alterations detected in other studies for the same tumor cell
by
lethalfang
▴ 90
There is some somatic SV study on these HCC1395: https://doi.org/10.1186/s13059-022-02816-6. There is also a CNA preprint from years ago b…
Comment: Somatic truth set
by
lethalfang
▴ 90
Just in case things are hard to find, for the WGS/WES, these are the data (what the file names mean: https://sites.google.com/view/seqc2/ho…
Comment: How to get subset of a Seurat object based on metadata?
by
jv
★ 1.0k
Hmm, not sure why `subset` doesn't match to '0:CD8 T cell' but maybe it's the ":"? An alternative option is the following: ``` idx <- whi…
Comment: GO enrichment analysis
by
Jeremy
▴ 780
g:Profiler has an Arabidopsis option: [g:Profiler][1] [1]: https://biit.cs.ut.ee/gprofiler/gost
Answer: Any tips in landing a bioinformatic job?
by
Carambakaracho
★ 3.2k
The first job is a nightmare and you will need some luck. I understand you're looking for an industry offering. Unfortunately I've worked o…
Traffic: 1679 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6