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594 results • Page
1 of 12
Sort: Rank
Rank
Views
Votes
Replies
0
votes
0
replies
14
views
Error with TCGA package - TCGAbiolinks
TCGA
r
cancer
1 hour ago by
JACKY
▴ 10
0
votes
0
replies
12
views
Question about rMATS output files
rMATS
novel
2 hours ago by
Lillian
• 0
0
votes
0
replies
50
views
Searching for RNA-seq melanoma samples in TCGA or cBioPortal
TCGA
r
cBioPortal
cancer
7 hours ago by
JACKY
▴ 10
1
vote
0
replies
102
views
gene strand determined by flag in read1 and read2 should be intepreted differently?
bam
flag
samtools
RNA-seq
1 day ago by
tomas4482
▴ 140
0
votes
0
replies
98
views
Estimating gene family size changes for a large tree?
Phylogenetics
CAFE5
20 hours ago by
Rijan
▴ 30
0
votes
1
reply
99
views
converting a DocumentTermMatrix to factor
DocumentTermMatrix
convert
factor
offtopic
updated 1 day ago by
cpad0112
20k • written 1 day ago by
hasani.iut6
▴ 70
0
votes
0
replies
80
views
Rooting a tree based on taxonomy of species
phylogeny
1 day ago by
lagartija
▴ 150
1
vote
0
replies
114
views
VQSR bias against rare variants
GATK
variant
VQSR
callling
1 day ago by
drabiza1
▴ 10
0
votes
0
replies
117
views
How do blastp step in MCScanX
MCScanX
1 day ago by
carlosgonzalezcruz327
▴ 10
0
votes
0
replies
99
views
How to count the number of multimapped reads from a bam file?
rnaseq
mapping
bowtie2
featurecounts
1 day ago by
O.rka
▴ 600
0
votes
2
replies
205
views
Best pipeline for RNAseq assembly and analysis (or help with stringtie assembly)
human
assembly
transcriptome
1 day ago by
Katherine
• 0
0
votes
3
replies
156
views
Incomplete alignment EMBOSS needleall
Alignment
updated 1 day ago by
lieven.sterck
14k • written 2 days ago by
bioinformatics.queries
▴ 50
0
votes
0
replies
94
views
TCGA data downloaded from GDC
TCGA
2 days ago by
Mamta
▴ 500
4
votes
4
replies
194
views
what is better to use in single cell
genomics
offtopic
updated 2 days ago by
Friederike
8.4k • written 2 days ago by
Bioinfo
• 0
0
votes
4
replies
213
views
Difference in number of DEGs from Deseq2 and limma-voom
RNAseq
Deseq2
limma
2 days ago by
dzisis1986
▴ 40
0
votes
2
replies
143
views
Gene expression assay Yeast sample prep
in
cells
Live
study
2 days ago by
dshdixit
▴ 10
1
vote
1
reply
173
views
ggscatter, gene expression, correlation
RNA-Seq
plot
scatter
updated 2 days ago by
Trivas
▴ 370 • written 2 days ago by
Rob
▴ 120
1
vote
0
replies
94
views
Interpretation of '1|2' in GT field and '0|1' in PGT field
GVCF
calling
GATK
SNP
VCF
2 days ago by
Choi
▴ 10
0
votes
0
replies
98
views
Cell Type and Gene Specific Survival Analysis in scRNA-seq
TCGA
scRNA-seq
2 days ago by
Ergün
▴ 20
0
votes
3
replies
173
views
how to get a sequence from read fragments with ref sequence?
reads
fragments
reference
assembly
sequence
updated 2 days ago by
Pierre Lindenbaum
146k • written 2 days ago by
Aaron
• 0
0
votes
1
reply
128
views
metagenome binning using MaxBin2 for each dataset
Metagenome
Binning
MaxBin
updated 2 days ago by
andres.firrincieli
★ 2.5k • written 2 days ago by
serene.s
• 0
1
vote
4
replies
245
views
bcftools index error: [E::bgzf_read_block] Invalid BGZF header at offset 12889964510
bcftools
updated 2 days ago by
cmdcolin
★ 2.2k • written 2 days ago by
biostars
▴ 10
0
votes
1
reply
154
views
Get cleaved SARS-CoV-2 protein sequences (all major variants)?
motif
variant
Python
SARS-CoV-2
cleavage
updated 2 days ago by
GenoMax
117k • written 2 days ago by
ngarber
• 0
0
votes
0
replies
141
views
How do I run pal2nal.pl in batch mode ?
gene
genome
protein
2 days ago by
Ramana
• 0
0
votes
0
replies
137
views
Converting GP field in a VCF to GT
VCF
GT
GP
2 days ago by
Garan
▴ 680
0
votes
0
replies
112
views
ReLERNN error
Genomics
ReLERNN
Problem
Error
2 days ago by
Javier
• 0
0
votes
0
replies
112
views
Hi-C data analysis (Loop and domain calling)
calling
and
Hi-C
HOMER
domain
Loop
2 days ago by
arsala521
▴ 10
0
votes
8
replies
300
views
How to ensure all fonts are written in Arial?
EnhancedVolcano
updated 2 days ago by
GenoMax
117k • written 3 days ago by
junli1988
• 0
2
votes
4
replies
197
views
Pymol superimposition by python script
python
superimposition
pymol
2 days ago by
iamsmor
• 0
0
votes
3
replies
142
views
splitting a bam file by XA tag into multiple lines
bam
tag
XA
file
BWA
2 days ago by
eric.londin
▴ 50
0
votes
2
replies
96
views
Bedtools Getfasta in snakemake
Bedtools
Snakemake
Wildcards
3 days ago by
Fadwa
▴ 10
0
votes
2
replies
152
views
gffcompare
RNA-seq
1 day ago by
linjc.xmu
▴ 30
0
votes
1
reply
95
views
Wildcards Snakemake using bedtools
Bedtools
Snakemake
Wildcards
updated 2 days ago by
Shred
▴ 640 • written 3 days ago by
Fadwa
▴ 10
0
votes
2
replies
95
views
Distinguish technical duplicates from biological duplicates
Duplicates
WGS
PCR
3 days ago by
BioStar22
• 0
0
votes
2
replies
99
views
SNPolisher_v1.5.0
AffyPipe
APT
Affymetrix
SNPolisher
2 days ago by
BiostarNewbie
• 0
0
votes
0
replies
68
views
How to (or not to) interpret the annotation abundance result from metagenome
gene
abundance
GO
annotation
metagenome
3 days ago by
young_bioinformatician
▴ 170
0
votes
1
reply
78
views
bwa mem for multiple paired read samples FastQfiles
fastq
reads
mem
bwa
paired
updated 3 days ago by
Pierre Lindenbaum
146k • written 3 days ago by
salman_96
▴ 50
0
votes
0
replies
68
views
StringTie values do not match mapped reads in Geneious
gene
rnaseq
Stringtie
expression
geneious
3 days ago by
Jirka
• 0
1
vote
1
reply
167
views
GATK 4.2.4.0 & GRCH38, exome pipeline gives plenty of false call? Is it problem of Haplotype caller? or else anything?
GATK
updated 3 days ago by
Pierre Lindenbaum
146k • written 3 days ago by
rajesh.msch
• 0
1
vote
1
reply
98
views
A modification of Nussinov's algorithm
coding
folding
rna
updated 3 days ago by
Joe
20k • written 3 days ago by
Yonatan
▴ 10
0
votes
1
reply
82
views
Ambiguous sequence Translation
biopython
translation
updated 3 days ago by
Hugo
▴ 360 • written 3 days ago by
usr2
• 0
0
votes
1
reply
78
views
looking for orthologue and paralogue
orthologue
updated 3 days ago by
lieven.sterck
14k • written 3 days ago by
mohamadzare6022
• 0
0
votes
0
replies
57
views
Taxonomic information for diamond blastp results
Taxonomy
blastp
diamond
3 days ago by
Utkarsha
• 0
0
votes
0
replies
98
views
How to choose the substitution models for each partition ?
gene
genome
protein
3 days ago by
sunnykevin97
▴ 760
1
vote
3
replies
198
views
Getting Organism information from PDB data
python
biopython
pdb
protein
updated 3 days ago by
cpad0112
20k • written 3 days ago by
drbioengineer
• 0
0
votes
0
replies
117
views
Mean Genotype File Format from PLINK PED (.bed+.bim+.fam) file set for gemma-wrapper
gemma
plink
qtl
gemma-wrapper
bimbam
3 days ago by
abc
• 0
1
vote
2
replies
111
views
FASTA file contains an unknown character error by rsem
an
rsem
error
character
unknown
updated 3 days ago by
Malcolm.Cook
★ 1.3k • written 4 days ago by
Lihe
▴ 10
0
votes
0
replies
59
views
smartpca from the Eigenstrat package
smartpca
EIG
MT
4 days ago by
Stefania
• 0
2
votes
12
replies
248
views
how to align an intron from a complex
genomic
updated 3 days ago by
WouterDeCoster
46k • written 4 days ago by
Bioinfo
• 0
0
votes
0
replies
69
views
Understanding ABI Sanger File Format
Sequencing
Sanger
Python
ABI
4 days ago by
oludhe
▴ 20
594 results • Page
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Recent Votes
Answer: ESTIMATE tumor purity
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Answer: Junior bioinformatician in italy
Junior bioinformatician in italy
A: Thoughts on switching from Biology to Bioinformatics
The Biostar Handbook. A bioinformatics e-book for beginners.
The Biostar Handbook. A bioinformatics e-book for beginners.
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▴ 10
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Recent Replies
Comment: Computing allele frequencies per individual for a set of SNPs in PLINK
by
chrchang523
9.3k
If 1kgtot.bim contains doubled IDs (e.g. "HG00096 HG00096", you need to provide a doubled ID to `--indv`. The log file shows that your scr…
Answer: ESTIMATE tumor purity
by
JACKY
▴ 10
Just in case anyone faces this problem in the future, here is the link for the updated `estimate package` on github: https://github.com/…
Answer: Exctract unmapped reads from BLAST
by
GenoMax
117k
> Is there a way to tell blast to output the unmapped reads into a > separate file? No there is not. You will need to use `-outfmt 7` whe…
Comment: Resfams Database usage
by
Neel
▴ 10
Hi, i have annotated genome by prokka and o want to search amr agaisnt resfams could you please tell me how to do it steps by steps.
Comment: Exctract unmapped reads from BLAST
by
Pierre Lindenbaum
146k
those are two distinct commands https://linux.die.net/man/1/comm and https://linux.die.net/man/1/join extract the names of your queries, s…
Comment: Exctract unmapped reads from BLAST
by
Pilar
• 0
Thanks for answering :), sorry, I'm new at this. These are commands I should use when I launch Blast in terminal?
Comment: Exctract unmapped reads from BLAST
by
Pierre Lindenbaum
146k
have a look at `comm` or `join`.
Comment: Computing allele frequencies per individual for a set of SNPs in PLINK
by
pifferdavide
▴ 100
Sorry I forgot to mention I used it in this loop, which worked with another plink file, but not with this 1KG file. for popfile in $(l…
Comment: Computing allele frequencies per individual for a set of SNPs in PLINK
by
chrchang523
9.3k
`--indv` takes a single ID, not a filename. So `--indv IDlist.txt` shouldn't work.
Answer: Quast Ouput
by
Ying
• 0
The "# contigs 47484" is based on contig of size >= 500 bp (see the first line of your screenshot). The "# contigs (>= 0 bp)" is the raw nu…
Answer: SRA obtained metagenomic reads appears to corrupt - cannot work on SingleM
by
Mensur Dlakic
★ 19k
Not sure why: 1) you are splitting reads; 2) you are fixing reads when they seem fine. I suggest something like this instead after `prefetc…
Comment: Quick way to get a field such as QNAME from the last read in a bam file?
by
kalavattam
▴ 70
Thank you. Do you know of any strategies for non-coordinated-sorted bam files?
Comment: analyzing a single cell and searching for a specific gene using single cell data
by
jared.andrews07
★ 13k
@rpolicastro gave you several helpful links for using the h5ad file, in addition to how to explore and plot data. The links in my answer al…
Comment: Computing allele frequencies per individual for a set of SNPs in PLINK
by
pifferdavide
▴ 100
I was able to run the --indv command successfully on a file with a bed file by simply listing the IDs in single column. However, with anoth…
Answer: Get nucleotide sequence of assembled RNA transcripts
by
tomas4482
▴ 140
1. design a RNA-seq experiment specifically seuqencing non-coding RNA/circRNA sequencing. 2. subset to interested region -> extract chi…
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