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316 results • Page
3 of 7
Sort: replies
Rank
Views
Votes
Replies
1
vote
2
replies
417
views
Ranked fold changes for non-replicated data
expression
metagenomics
LogFC
fold-change
updated 8 days ago by
Ram
40k • written 18 months ago by
Tom
▴ 540
0
votes
2
replies
278
views
Deseq2 colData for single condition
RNA-seq
7 days ago by
Petesview
• 0
1
vote
2
replies
255
views
Multiple testing adjustment for stepwise model selection
statistics
5 days ago by
mel22
▴ 100
0
votes
2
replies
205
views
I am interested in creating bar graphs from the outcomes of my BLAST analysis
Blastn
updated 17 days ago by
Ram
40k • written 17 days ago by
hashim.rana11
▴ 20
0
votes
2
replies
266
views
Gene Ontology - How to do Transcript Ontology?
gene-ontology
GO
transcripts
updated 12 days ago by
Ram
40k • written 13 days ago by
Lakritz the LabRat
• 0
0
votes
2
replies
1.5k
views
Question about REDItools
REDItools
updated 7 days ago by
Ethan Lee
• 0 • written 4.1 years ago by
tujuchuanli
▴ 100
0
votes
2
replies
216
views
Is it okay to just average the log2FC values across different cell types in pseudobulk scRNA-seq data?
scrna-seq
rna-seq
log2fc
3 days ago by
JLee
• 0
0
votes
2
replies
1.9k
views
CWL :File created on fly by one step of a workflow as input to another step
Common-Workflow-Language
CWL
updated 11 days ago by
Ram
40k • written 6.5 years ago by
akashmdr90
• 0
1
vote
2
replies
220
views
Searching a tool to modify annotation files.
annotation
updated 12 days ago by
GenoMax
134k • written 12 days ago by
Charles Plessy
★ 2.9k
0
votes
2
replies
282
views
ClusterProfiler GeneRatio demoninator not the same for all values
enrichment-analysis
clusterprofiler
RNA-seq
deseq2
16 days ago by
Lily
• 0
0
votes
2
replies
244
views
Change mounted drive on GCP Batch nextflow process
google-cloud-platform
unix
nextflow
27 days ago by
dthorbur
▴ 550
0
votes
2
replies
249
views
Extract reference and alternate SNPs for different samples
vcftools
bcftools
SNPs
updated 15 days ago by
GenoMax
134k • written 16 days ago by
gubrins
▴ 270
0
votes
2
replies
287
views
Sentrix Array Genotyping
Illumina
15 days ago by
joo
• 0
0
votes
2
replies
305
views
Confusion about transcript ablation
Ensembl
Variation
VEP
updated 29 days ago by
Ben_Ensembl
★ 2.3k • written 4 weeks ago by
Heiha
• 0
0
votes
2
replies
678
views
pal2nal -nogap -nomismatch options not working
pal2nal
updated 7 days ago by
wangjb702
• 0 • written 10 months ago by
pkfsantos
• 0
0
votes
2
replies
248
views
circRNA isolation
circular-RNA
circRNA
cDNA
8 days ago by
dbagmerve
• 0
0
votes
2
replies
548
views
Network analysis for two Factors
chip-seq
network-analysis
updated 22 days ago by
Ram
40k • written 7 months ago by
Ankit
▴ 390
0
votes
2
replies
230
views
Segmentation fault error in CONTROL-FREEC
copy-number-variants
FREEC
11 days ago by
DdogBoss
• 0
1
vote
2
replies
292
views
Converting scMultiome data to loom using SEURAT
scMultiome
CCAF
Seurat
14 days ago by
halimaakhter014
• 0
0
votes
2
replies
236
views
Volunteer projects in 2023 for new bioinformaticians
junior
volunteer
projects
15 days ago by
gallardodiazmiriam
▴ 20
0
votes
2
replies
329
views
What is happening in the Zuker-Algorithm
R
folding
RNA
updated 21 days ago by
4galaxy77
2.8k • written 21 days ago by
Serij´s
• 0
1
vote
2
replies
278
views
Adjusting covariate in regression model
regression
statistics
23 days ago by
Jonathan Yoou
▴ 60
0
votes
2
replies
221
views
How to run TRUST4 for BCR/TCR detection using 10X data?
immunology
trust4
illumina
10x
11 days ago by
O.rka
▴ 680
0
votes
2
replies
373
views
log2fC from limma on methylation analysis
methylation
limma
minfi
fold-change
updated 8 days ago by
Ram
40k • written 11 months ago by
adeizadavid
▴ 10
2
votes
2
replies
343
views
Currently lost between contigs, scaffolds and chromosomes due to positionally sorted scRNAseq bam files
bam
contigs
scRNAseq
updated 18 days ago by
ATpoint
76k • written 18 days ago by
nyxtoviopouli
• 0
1
vote
2
replies
212
views
Problem with Mageck paired analysis
Mageck
updated 12 days ago by
Meisam
▴ 200 • written 12 days ago by
toma.85
• 0
2
votes
2
replies
253
views
Pangenome using Orthofinder
OrthoFinder
Pangenome
bacteria
5 days ago by
kirankumareripogu
▴ 10
0
votes
2
replies
240
views
Assigning GO ids to blast hits
Linux
GO
blast
15 days ago by
Mousumi Akter
• 0
0
votes
2
replies
180
views
Identify genes for mapped reads with combined human-7HPV genome index
star
samtools
updated 4 days ago by
Ram
40k • written 5 days ago by
r.j.lock
• 0
0
votes
1
reply
166
views
MAKER - How to set weight for merge legacy annotations
maker
annotation
updated 23 days ago by
Juke34
8.2k • written 23 days ago by
1300189805
• 0
0
votes
1
reply
168
views
Bowtie2 Error: reads file does not look like a FASTQ file
bowtie2
alignment
updated 17 days ago by
Ram
40k • written 17 days ago by
SO_Bio
• 0
0
votes
1
reply
104
views
Understanding TCGA barcodes with dot in the fieldname
tcga
updated 18 hours ago by
Ram
40k • written 19 hours ago by
Tahsin
• 0
1
vote
1
reply
236
views
Bioconductor Package Installation
bioconductor
updated 8 days ago by
Ram
40k • written 8 days ago by
oduduabasi.isaiah
• 0
0
votes
1
reply
173
views
Drawing label around clusters
cells
Single
updated 11 days ago by
Mensur Dlakic
★ 24k • written 11 days ago by
synat.keam
▴ 80
0
votes
1
reply
229
views
Data normalization and analysis
linux
drug
SARS-Cov2
updated 16 days ago by
1769mkc
★ 1.1k • written 3 months ago by
Adyasha
• 0
0
votes
1
reply
193
views
Annotation of L1 from short read (single-strand) RNA
short-read
Line1
fastq
annotation
updated 25 days ago by
rfran010
▴ 790 • written 25 days ago by
Kilian
• 0
0
votes
1
reply
1.0k
views
Downstream analysis on multi-sample or single-sample VCF files?
next-gen
VCF
updated 7 days ago by
Ram
40k • written 2.8 years ago by
NGSCanBioinf
▴ 10
0
votes
1
reply
1.4k
views
NS, AC, AN and AF in VCF file
VCF
updated 18 days ago by
Mwangana
• 0 • written 14 months ago by
wangdp123
▴ 310
1
vote
1
reply
200
views
Inclusive vs. Separate Approaches for Gene Ontology Enrichment Analysis
Gene-Ontology
GO-terms
Enrichment
updated 24 days ago by
rfran010
▴ 790 • written 24 days ago by
Biomed-jeh
• 0
0
votes
1
reply
140
views
How to download genomes and proteins from JGI in bulk via the command line?
jgi
cli
updated 1 day ago by
Ram
40k • written 1 day ago by
O.rka
▴ 680
0
votes
1
reply
308
views
txt files in minfi
differential-methylation-analysis
updated 9 days ago by
Ram
40k • written 5 weeks ago by
ananta.kapoor
• 0
0
votes
1
reply
67
views
Hugo_Symbol to Entrez ID
hugo
bioinformatics
entrez
ids
symbols
updated 30 minutes ago by
GenoMax
134k • written 12 hours ago by
shakyaram079
• 0
0
votes
1
reply
265
views
computeMatrix error: [pyBwOpen] bw is NULL!
deeptools
bigWig
computeMatrix
updated 19 days ago by
rfran010
▴ 790 • written 21 days ago by
bp22
▴ 30
0
votes
1
reply
215
views
Biopython and Fab detection
biopython
fab-detection
antibody
updated 22 days ago by
Jesse
▴ 720 • written 23 days ago by
PamCraven
• 0
0
votes
1
reply
180
views
Merge microbiota data
microbiota
OTU
updated 16 days ago by
Ram
40k • written 16 days ago by
shamila.ismael
• 0
0
votes
1
reply
174
views
Replace ambiguous characters in fasta MSA
maximum-likelihood
DNA
updated 16 days ago by
Joe
21k • written 17 days ago by
Alexandre
• 0
0
votes
1
reply
168
views
Identify parent of each read in a GAF
gaf
vgteam
vg
updated 3 days ago by
Jordan M Eizenga
▴ 360 • written 4 days ago by
cfourps
▴ 10
0
votes
1
reply
898
views
Issue interpreting plasmidSPAdes output
plasmidSPAdes
updated 2 days ago by
Ram
40k • written 2.9 years ago by
A_heath
▴ 140
0
votes
1
reply
220
views
cuffmerge erorr "ValueError: can't have unbuffered text I/O"
cuffmerge
updated 26 days ago by
Ram
40k • written 27 days ago by
khaldari.i
• 0
1
vote
1
reply
476
views
Haplogrep classify error
haplogroup
mitochondria
haplogrep
updated 12 days ago by
angelika_95
• 0 • written 24 months ago by
jdru
▴ 10
316 results • Page
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Recent Votes
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
Comment: Error in bowtie2
Recommended Tools For Alternative Splicing Detection From Rna-Seq Data
Comment: Using STAR aligner to build index of hg38
Answer: How to sort using samtools
The Biostar Handbook. A bioinformatics e-book for beginners.
The Biostar Handbook. A bioinformatics e-book for beginners.
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Recent Replies
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I tried the commands you recommended in the bash/command line. When I tried `find "${bam_directory}" -type f -name "*.bam" | xargs samtools…
Comment: Hugo_Symbol to Entrez ID
by
GenoMax
134k
Can you provide some examples of HUGO ID's you are unable to convert?
Comment: Using STAR aligner to build index of hg38
by
GenoMax
134k
GTF file includes gene models/annotation information. You could also use a program like `salmon/kallisto` with just human transcriptome to…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
then test each files. find "${bam_directory}" -type f -name "*.bam" | xargs samtools quickcheck file "${reference_gen…
Answer: Ties in reranked list
by
alserg
▴ 840
The problem here is not the ties, but that your gene IDs in the pathway list (ensemble human genes) does not match the names of the stats v…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I did not use the space between the `-u` flag and the `-o` flag (the blank line) but I did do what you recommended: # Run CuffDiff wit…
Answer: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
how about simply trying: ``` cuffdiff -u "${annotation_gtf}" \ -o "${output_directory}" \ -b "${reference_genome}" \ -p "${num_th…
Comment: Using STAR aligner to build index of hg38
by
Grace
• 0
Yes, I know also need a GTF file. So the GTF file will include RNA information? Thanks for your answer!!
Comment: STAR Intron Motif Script Gives Segmentation fault Error
by
Y
• 0
I will try and figure it out on my own given what you all have mentioned. Thank you for your time.
Comment: Using STAR aligner to build index of hg38
by
Amitm
★ 2.2k
Have you gone through the manual [here][1]. In addition to the genome fasta file, you would also need a GTF file (of gene annotations) to b…
Comment: Highly inflated p-values in GWAS by regenie
by
4galaxy77
2.8k
You still definitely need to include principle components as covariates, even if your data is from the same ethnicity. Not including those …
Answer: How to create structural variants ground truth for alignment of two long-read ge
by
Christophe
• 0
Hi, D-genies is using minimap2 to align both genomes and minimap2 is chaining local alignments to produce a global one. If the SV are sm…
Comment: Ties in reranked list
by
Hamza
• 0
In this case there are no results in my fgsea object unfortunately
Comment: Genes with promoter and enhancer regions as GTF
by
abis.1819104
• 0
how to generate promoters.bed and enhancer.bed files from gtf file
Comment: Deepvariant variant calling by singularity
by
jeffhsu3
• 0
I am having a similar issue was this ever resolved? Getting this error: I0922 22:22:08.794076 140356687390528 make_examples_core.py:257] O…
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