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88 results • Page
2 of 2
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Votes
Replies
0
votes
1
reply
898
views
Issue interpreting plasmidSPAdes output
plasmidSPAdes
updated 2 days ago by
Ram
40k • written 2.9 years ago by
A_heath
▴ 140
2
votes
1
reply
162
views
Best Tools and Practices for CNV Variant Calling in WES Data
CNV
WES
updated 5 days ago by
Pierre Lindenbaum
157k • written 5 days ago by
Nadav
▴ 10
0
votes
1
reply
376
views
Close genome comparison
Mauve
Mugsy
genome-comparison
GSAlign
updated 2 days ago by
Ram
40k • written 19 months ago by
A_heath
▴ 140
1
vote
1
reply
177
views
Read Counts from BAM file
bam
samtools
qualimap
readcount
updated 3 days ago by
ATpoint
76k • written 3 days ago by
Smriti
• 0
0
votes
1
reply
141
views
How to download genomes and proteins from JGI in bulk via the command line?
jgi
cli
updated 1 day ago by
Ram
40k • written 1 day ago by
O.rka
▴ 680
0
votes
1
reply
143
views
OMA in AWS cloud
OMA
AWS
updated 1 day ago by
Ram
40k • written 2 days ago by
Ksel
• 0
0
votes
1
reply
129
views
Modify the code to take most abundant reads from a cluster and process it.
cd-hit-est
clustering
updated 4 hours ago by
Mensur Dlakic
★ 24k • written 17 hours ago by
Mohd
▴ 40
0
votes
1
reply
152
views
vg rna pantranscriptome
rna
vg
updated 4 days ago by
Jordan M Eizenga
▴ 360 • written 4 days ago by
z
• 0
0
votes
1
reply
133
views
High amount of intronic/intergenic reads in SMARTer stranded total bulk RNAseq
RNA-seq
DNA
SMARTer
updated 1 day ago by
Ram
40k • written 1 day ago by
Mat
▴ 60
0
votes
1
reply
754
views
How to identify specific region using Mauve?
Mauve
primers
updated 2 days ago by
Ram
40k • written 2.8 years ago by
A_heath
▴ 140
0
votes
1
reply
105
views
Understanding TCGA barcodes with dot in the fieldname
tcga
updated 19 hours ago by
Ram
40k • written 20 hours ago by
Tahsin
• 0
0
votes
0
replies
167
views
PopGenome: there are missing regions when calculating Tajima's D per gene
PopGenome
6 days ago by
Bing
• 0
1
vote
0
replies
319
views
Strand orientation and GC skew
CGView
strand-orientation
GC-skew
updated 2 days ago by
Ram
40k • written 13 months ago by
A_heath
▴ 140
0
votes
0
replies
89
views
Why dbConnect GEOmetadb_demo.sqlite shows some information while dbConnect GEOmetadb.sqlite shows no information
R
GEO
GEOmetadb
updated 1 day ago by
Pierre Lindenbaum
157k • written 1 day ago by
nonaddldy
▴ 10
0
votes
0
replies
112
views
Genetic distance in cM from VCF of non-reference species to run Beagle
plink
beagle
vcf
updated 4 days ago by
Ram
40k • written 4 days ago by
AndrMod
• 0
0
votes
0
replies
32
views
My rMATS output file is not showing gene symbols and only XLOC gene ID are being shown.
rMATS
rMATS-turbo
2 hours ago by
Nipan
• 0
0
votes
0
replies
75
views
bedGraph for Coverage of Insert from Paired-End Data?
genome
awk
coverage
bed
paired-end
22 hours ago by
vanbelj
▴ 40
0
votes
0
replies
117
views
Data for pathview() (KEGG Pathway Map) after EdgeR
EdgeR
LogFC
pathview
KEGG
5 days ago by
Ann
▴ 10
0
votes
0
replies
142
views
Why we are using filtering >0 for up and <0 for down after TopTags() to extract de genes ids?
EdgeR
logfc
DEG
5 days ago by
Ann
▴ 10
0
votes
0
replies
132
views
Homozygous reference genotype for a GIAB genome
GIAB
IDs
reference
rs
Homozygous
6 days ago by
New2R
▴ 60
0
votes
0
replies
138
views
PRSice - Phenotype File Not Read Correctly
PRSice2
6 days ago by
V
• 0
0
votes
0
replies
356
views
Distinction of two very close bacterial strains
mutations
bacteria
updated 2 days ago by
Ram
40k • written 2.3 years ago by
A_heath
▴ 140
0
votes
0
replies
131
views
Illumina methylation EPIC V2 array
ewas
methylation
enrichment
missMethyl
EPIC
2 days ago by
juliviglino
• 0
0
votes
0
replies
104
views
Any way to predict hormone biosynthesis pathways in understudied organisms?
genomics
orthologs
biosynthesis-pathway
updated 3 days ago by
Ram
40k • written 3 days ago by
Mark
• 0
0
votes
0
replies
263
views
GTF/Reference genome adjustments? Gene-based differential expression analysis of genetically modified mouse line
RNA-seq
DGE
updated 5 days ago by
Ram
40k • written 5 days ago by
alex
• 0
0
votes
0
replies
121
views
maftools - median survival time doesn't match plot
R
survival
maftools
K-M
4 days ago by
BioGuy
• 0
0
votes
0
replies
78
views
Compare peaks between clusters in sc-ATAC
Peak-Calling
Epigenome
scATAC-seq
updated 19 hours ago by
Ram
40k • written 20 hours ago by
Ahmed.waraky
▴ 10
2
votes
0
replies
132
views
the dispersion estimation of edgeR and DESeq2
estimation
dipersion
edgeR
DESeq2
4 days ago by
tommy
▴ 30
0
votes
0
replies
110
views
Current landscape of approaches to scRNA-seq with nanopore sequencers?
scRNA-seq
nanopore
single-cell
updated 1 day ago by
Ram
40k • written 1 day ago by
LauferVA
3.7k
0
votes
0
replies
99
views
Gene enrichment analysis of prokaryotes gene
prokaryotes
GO
enrichment
DEG
1 day ago by
Genta
• 0
0
votes
0
replies
63
views
Convert RNASeq V2 data in cBioPortal having RSEM normalized results to TPM values
bioinformatics
TPM
RNASeqV2
cbioportal
RSEM
updated 2 hours ago by
4galaxy77
2.8k • written 11 hours ago by
shakyaram079
• 0
0
votes
0
replies
423
views
after gatk VariantAnnotator -V *_com_norm.vcf -A AlleleFraction -O *_norm_AB.vcf There "nan,nan" or "nan" in my vcf file
gatk
AlleleFraction
VariantAnnotator
updated 4 days ago by
Ram
40k • written 4 days ago by
zhuo
• 0
0
votes
0
replies
128
views
cosine simialrity of mutation signature analysis
similarity
Mutational
analysis
signature
cosine
5 days ago by
probioquestioner
• 0
0
votes
0
replies
109
views
High pvalues when using clusterProfiler for seurat
clusterProfiler
1 day ago by
Ahmed
• 0
0
votes
0
replies
175
views
What metrics to use to calculate variant library evenness or uniformity or bias
library-bias
variant
updated 6 days ago by
Ram
40k • written 7 days ago by
eli_bayat
▴ 90
0
votes
0
replies
136
views
Selecting SNPs for two-sample Mendelian randomization study/analysis
Two-sample
SNPs
Mendelian
randomization
5 days ago by
huynguyenacademia
• 0
0
votes
0
replies
588
views
Contig extension using PRICE
price
updated 2 days ago by
Ram
40k • written 3.0 years ago by
A_heath
▴ 140
0
votes
0
replies
105
views
scanpy problem for empty cells
scanpy
updated 1 day ago by
Ram
40k • written 1 day ago by
Andy
▴ 90
88 results • Page
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Recent Votes
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
Comment: Error in bowtie2
Recommended Tools For Alternative Splicing Detection From Rna-Seq Data
Comment: Using STAR aligner to build index of hg38
Answer: How to sort using samtools
The Biostar Handbook. A bioinformatics e-book for beginners.
The Biostar Handbook. A bioinformatics e-book for beginners.
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Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I tried the commands you recommended in the bash/command line. When I tried `find "${bam_directory}" -type f -name "*.bam" | xargs samtools…
Comment: Hugo_Symbol to Entrez ID
by
GenoMax
134k
Can you provide some examples of HUGO ID's you are unable to convert?
Comment: Using STAR aligner to build index of hg38
by
GenoMax
134k
GTF file includes gene models/annotation information. You could also use a program like `salmon/kallisto` with just human transcriptome to…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
then test each files. find "${bam_directory}" -type f -name "*.bam" | xargs samtools quickcheck file "${reference_gen…
Answer: Ties in reranked list
by
alserg
▴ 840
The problem here is not the ties, but that your gene IDs in the pathway list (ensemble human genes) does not match the names of the stats v…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I did not use the space between the `-u` flag and the `-o` flag (the blank line) but I did do what you recommended: # Run CuffDiff wit…
Answer: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
how about simply trying: ``` cuffdiff -u "${annotation_gtf}" \ -o "${output_directory}" \ -b "${reference_genome}" \ -p "${num_th…
Comment: Using STAR aligner to build index of hg38
by
Grace
• 0
Yes, I know also need a GTF file. So the GTF file will include RNA information? Thanks for your answer!!
Comment: STAR Intron Motif Script Gives Segmentation fault Error
by
Y
• 0
I will try and figure it out on my own given what you all have mentioned. Thank you for your time.
Comment: Using STAR aligner to build index of hg38
by
Amitm
★ 2.2k
Have you gone through the manual [here][1]. In addition to the genome fasta file, you would also need a GTF file (of gene annotations) to b…
Comment: Highly inflated p-values in GWAS by regenie
by
4galaxy77
2.8k
You still definitely need to include principle components as covariates, even if your data is from the same ethnicity. Not including those …
Answer: How to create structural variants ground truth for alignment of two long-read ge
by
Christophe
• 0
Hi, D-genies is using minimap2 to align both genomes and minimap2 is chaining local alignments to produce a global one. If the SV are sm…
Comment: Ties in reranked list
by
Hamza
• 0
In this case there are no results in my fgsea object unfortunately
Comment: Genes with promoter and enhancer regions as GTF
by
abis.1819104
• 0
how to generate promoters.bed and enhancer.bed files from gtf file
Comment: Deepvariant variant calling by singularity
by
jeffhsu3
• 0
I am having a similar issue was this ever resolved? Getting this error: I0922 22:22:08.794076 140356687390528 make_examples_core.py:257] O…
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