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Limit : this year
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1,000 results • Page
2 of 20
Sort: replies
Rank
Views
Votes
Replies
7
votes
12
replies
1.6k
views
How to pass arguments in a bash script to perform read alignment to the reference?
exome
bwa
bash
updated 5 months ago by
Ram
43k • written 4.2 years ago by
Karma
▴ 310
2
votes
12
replies
1.7k
views
gatk Funcotator
vcf
Funcotator
gatk
9 months ago by
Saurabh
• 0
6
votes
12
replies
2.0k
views
Help with error in cellranger
Cellranger
updated 11 months ago by
GenoMax
141k • written 11 months ago by
Chris
▴ 260
0
votes
12
replies
3.3k
views
comparative analysis of two strains of bacteria's genomes
genome
gene
updated 11 months ago by
Ram
43k • written 6.4 years ago by
fonteneaudam
▴ 20
4
votes
12
replies
7.7k
views
6 follow
BWA error: no ID within the read group line
bwa
readgroups
RG
parallel
updated 10 weeks ago by
mgdrnl
▴ 10 • written 3.3 years ago by
whb
▴ 60
0
votes
12
replies
2.4k
views
Differential gene expression analysis with no replicates using edgeR
edgeR
RNA-seq
DEG
updated 4 months ago by
Sajad
▴ 90 • written 16 months ago by
Apex92
▴ 280
3
votes
12
replies
473
views
struggle to get fasta files from ucsc goldenPath
ucsc
getfasta
fasta
16 days ago by
Lila M
★ 1.2k
0
votes
11
replies
1.6k
views
count file having zero
RNA-Seq
next-gen
alignment
updated 10 months ago by
Ram
43k • written 5.2 years ago by
BioBaby
▴ 20
2
votes
11
replies
728
views
Reference Guided genome assembly
tabix
updated 7 weeks ago by
Michael
54k • written 8 weeks ago by
sainavyav22
• 0
4
votes
11
replies
606
views
Remove X in row name with only number
GSVA
updated 10 weeks ago by
Ram
43k • written 10 weeks ago by
Chris
▴ 260
5
votes
11
replies
986
views
Make heatmap for RNA-seq with non replicate
RNA-seq
updated 8 months ago by
Ram
43k • written 8 months ago by
Chris
▴ 260
2
votes
11
replies
5.2k
views
How long it takes to run repeat masker on a full genome
Assembly
updated 3 months ago by
e.r.zakiev
▴ 190 • written 7.8 years ago by
CAnna
▴ 20
0
votes
11
replies
1.1k
views
Normalization for microarrays >1000+ samples?
microarray
normalization
oligo
7 months ago by
evmae
• 0
2
votes
11
replies
1.0k
views
Data preparation for a ML model
machine-learning
RNA-Seq
R
python
biomarkers
4 months ago by
sil_bioinfo
▴ 40
5
votes
11
replies
1.3k
views
Interpret IGV output inversion
variant-calling
IGV
alignment
updated 8 months ago by
cmdcolin
★ 3.8k • written 8 months ago by
pablo
▴ 300
0
votes
11
replies
769
views
Filtering VCF based on codon-transcript
vcf
bcftools
updated 4 weeks ago by
barslmn
★ 2.1k • written 6 weeks ago by
avelarbio46
▴ 30
1
vote
11
replies
1.2k
views
Quantification after transcriptome assembly with Trinity
De-novo-transcriptome-assembly
Trinity
RNA-seq-analysis
updated 6 months ago by
GenoMax
141k • written 7 months ago by
langziv
▴ 50
4
votes
11
replies
1.6k
views
Check Strandedness
Check_Strandedness
how_are_we_stranded_here
leafcutter
bash
updated 6 months ago by
Brian Bushnell
20k • written 8 months ago by
Y
• 0
4
votes
11
replies
1.7k
views
PCA WITH PLINK
PLINK
updated 8 months ago by
Kevin Blighe
87k • written 8 months ago by
Michelle Guerra
• 0
0
votes
11
replies
2.2k
views
i want to convert .ht2 files into FASTA file
GATK
HISAT2
FASTA
updated 10 months ago by
Ram
43k • written 4.9 years ago by
saumya.sharma1509
• 0
2
votes
10
replies
3.2k
views
6 follow
Split plink files by a number of SNPs
GWAS
SNP
plink
updated 6 months ago by
Raygozak
★ 1.4k • written 5.8 years ago by
kakukeshi
▴ 80
2
votes
10
replies
785
views
Only one read per run - Trying to use CellRangerv7
Fastq
CellRanger
updated 21 days ago by
Ram
43k • written 8 weeks ago by
Sky
▴ 10
0
votes
10
replies
922
views
ONT Raw Read Sequencing Pipeline
remora
dorado
ONT
Basecall
3 months ago by
turcoa1
• 0
1
vote
10
replies
964
views
file conversion from gtf to gff3 for evidence modeler
gff3
stringtie
gtf
6 months ago by
rj.rezwan
• 0
5
votes
10
replies
836
views
Error when viewing a bam file
samtools
updated 10 months ago by
Ram
43k • written 11 months ago by
Chris
▴ 260
1
vote
10
replies
1.2k
views
Coverage histogram query
Alignment
7 months ago by
prasundutta87
▴ 660
0
votes
10
replies
584
views
Nextflow ERROR : Timeout waiting for connection from pool
nextflow
17 days ago by
dzisis1986
▴ 70
1
vote
10
replies
1.1k
views
Do monoclonal antibodies have a blocking effect?
block
antibody
offtopic
updated 8 months ago by
Jeremy
▴ 880 • written 8 months ago by
jinyu
▴ 10
0
votes
10
replies
843
views
Problem running scPred
scPred
scPredict
updated 5 weeks ago by
Ram
43k • written 3 months ago by
Nitin
• 0
3
votes
10
replies
1.2k
views
STAR index not working
STAR
RNA-Seq
7 months ago by
camillab.
▴ 160
5
votes
10
replies
1.2k
views
the distribution of log2Fc and t value are not similar for bulk RNA-seq
decoupleR
RNA-seq
7 months ago by
alwayshope
▴ 40
1
vote
10
replies
1.7k
views
Deepvariant variant calling by singularity
deepvariant
singularity
conda
docker
updated 6 months ago by
jeffhsu3
• 0 • written 9 months ago by
fra.r.silvestro
▴ 10
2
votes
10
replies
3.4k
views
RepeatMasker: createLib() Error
RepeatMasker
updated 1 day ago by
Ram
43k • written 7.4 years ago by
linda
• 0
14
votes
10
replies
880
views
How to remove center population from seurat cluster
single-cell
4 months ago by
synat.keam
▴ 100
1
vote
10
replies
3.1k
views
How to specify/calculate subject coverage of the alignment (alignment of query[protein sequence] and subject[Nucleotide sequence]) in tBLASTn?
alignment
BLAST
tBLASTn
updated 10 months ago by
Ram
43k • written 5.1 years ago by
Kumar
▴ 120
8
votes
10
replies
2.0k
views
PyWGCNA
WGCNA
10 months ago by
yoshifumimiya
▴ 40
6
votes
10
replies
1.7k
views
Integration of Microarray datasets with different platforms and biological groups
limma
microarray
DEA
batch-effect
updated 1 day ago by
Ram
43k • written 4.7 years ago by
asalimih
▴ 60
8
votes
10
replies
652
views
bash script
whole-genome-sequencing
updated 3 days ago by
Ram
43k • written 10 days ago by
bestone
▴ 30
0
votes
9
replies
463
views
Low number of both surviving reads after trimming
trimmomatic
trimming
21 days ago by
Jay
• 0
6
votes
9
replies
2.0k
views
Error when runing Bowtie2: (ERR): bowtie2-align exited with value 1
BAM
Bowtie
updated 11 months ago by
Ram
43k • written 14 months ago by
luzglongoria
▴ 50
0
votes
9
replies
544
views
99.9999% of Q30 bases is normal?
RNA-seq
fastp
updated 7 days ago by
LauferVA
4.2k • written 8 days ago by
Aki
▴ 10
0
votes
9
replies
1.5k
views
Chipseq analysis on repeat genes
galaxy
repeatmasker
ChIP-seq
repeat-elements
ATAC-seq
updated 6 months ago by
rfran010
▴ 900 • written 7 months ago by
pb11
▴ 10
2
votes
9
replies
1.2k
views
Salmon (or other pseudo-mappers) for multi-species RNAseq read filtering
STAR
Salmon
mapper
aligner
RNAseq
4 months ago by
ian.will
▴ 30
4
votes
9
replies
1.1k
views
Snakemake issue with wrappers
Python
Snakemake
5 months ago by
Begonia_pavonina
▴ 150
1
vote
9
replies
2.1k
views
kallisto bootstrap / condo installation problem
hfd5
kallisto
condo
updated 11 months ago by
dsull
★ 5.8k • written 11 months ago by
micecanfly
▴ 10
0
votes
9
replies
5.4k
views
Calculating FPKM and TPM by hand from htseq-count output?
RNA-Seq
rna-seq
htseq
FPKM
TPM
updated 8 months ago by
ATpoint
81k • written 5.0 years ago by
Joel Wallenius
▴ 210
0
votes
9
replies
828
views
Deseq2 error
R
deseq2
6 months ago by
sgadila
• 0
1
vote
9
replies
843
views
Python3 or Ubuntu, not perl: Have Primers with Degenerate Bases, Need tool or way to List all Possible Nucleotide Sequences
degenerate-bases
python
primers
biopython
updated 5 weeks ago by
Dave Carlson
★ 1.7k • written 5 weeks ago by
Matthew
• 0
4
votes
9
replies
983
views
bcl2fastq troubleshooting all reads dumped to "Undetermined"
demultiplex
illumina
sequencing
updated 4 months ago by
GenoMax
141k • written 4 months ago by
MaxF
▴ 120
7
votes
9
replies
1.1k
views
Calculation of TMB on gene level
genomics
updated 6 months ago by
svp
▴ 680 • written 6 months ago by
smrutimayipanda
▴ 20
1,000 results • Page
2 of 20
Recent Votes
Answer: Korean reference file
Comment: Korean human genome reference file
C: samtools sort bam file
A: samtools sort bam file
Answer: Downloaded pdb's on rcsb.org
Comment: Downloaded pdb's on rcsb.org
Comment: Downloaded pdb's on rcsb.org
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★ 2.0k
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Recent Replies
Comment: Load a full GFF3 into annotation track using arrow (Apollo)
by
cmdcolin
★ 3.8k
Hi there, I let a developer know of this thread and they said they can check it out next week. Feel free to post to the https://github.com/…
Comment: How to get the reference panel for UKBB
by
航太郎
• 0
Thanks for your guide!
Comment: Removing duplicates
by
joe
▴ 500
I'd try those OP @samuel recommends My guess is you're new to ONT, fast5 is the 'raw data' which is current over time...other than generat…
Comment: RNA seq differential expression analysis
by
swbarnes2
14k
No. Find a tutorial, try it, then if you have specific questions, ask of the galaxy help site.
Answer: Adding CB tag to bam file
by
swbarnes2
14k
Shouldn't you use PicardTools for this?
Comment: Downloaded pdb's on rcsb.org
by
Ram
43k
Sure. Or, one could do: bash try.bash "Homo sapiens" AHR | xargs -I v_pdb curl -s -o v_pdb.pdb https://files.rcsb.org/view/v_pdb.pdb
Comment: Downloaded pdb's on rcsb.org
by
iamsmor
• 0
thank you so much
Comment: Downloaded pdb's on rcsb.org
by
GenoMax
141k
This may be the best option. Get the PDB ID's $ ./get.sh "Mus musculus" AHR 4M4X 5NJ8 5V0L 8H77 Then use `curl` to g…
Comment: Downloaded pdb's on rcsb.org
by
Ram
43k
It gives you the first 25 results though. I'll see if I can change that. EDIT: I've updated that number to 250. I'm hoping you won't need …
Comment: Downloaded pdb's on rcsb.org
by
iamsmor
• 0
thank you very much
Answer: Downloaded pdb's on rcsb.org
by
Ram
43k
I'm going to build off of OP's query and give them a simple script: ```bash organism=$(echo $1 | sed 's/ /%20/g') gene=$2 curl -s https:/…
Comment: miRNAs quantification using mirdeep2 tool
by
GenoMax
141k
This question does not make complete sense. If you have your own data files why do you need deep sequencing reads? Genome (can be download…
Comment: Downloaded pdb's on rcsb.org
by
GenoMax
141k
For a non-programmer using the search builder link included above may be the best option. Even that is not very user friendly.
Comment: Downloaded pdb's on rcsb.org
by
Ram
43k
That's how they seem to have written their query - automating that is a bit of a pain though as it takes a crazy JSON as input.
Comment: Downloaded pdb's on rcsb.org
by
GenoMax
141k
You can use the "Advanced query" builder (https://www.rcsb.org/search/advanced ) to create a query like: https://www.rcsb.org/search?requ…
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