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1,000 results • Page
2 of 20
Sort: Rank
Rank
Views
Votes
Replies
1
vote
5
replies
232
views
bcftools error merging two VCFs: REF prefixes differ
bcftools
VCF
6 days ago by
Shane
• 0
0
votes
2
replies
878
views
RSEM not giving .genes.results and isoforms.results ; Plase check if you provide correct parameters/options for the pipeline!
genomics
transcriptome
rsem
ngs
4 days ago by
kat.bi
• 0
0
votes
2
replies
212
views
how to create a loop in R
R
offtopic
updated 6 days ago by
Ram
40k • written 6 days ago by
mthm
▴ 50
0
votes
1
reply
169
views
GO analysis after DESeq2
GO
updated 7 days ago by
Ram
40k • written 7 days ago by
oduduabasi.isaiah
• 0
3
votes
2
replies
202
views
Program for Overlapping DMRs (Differentially Methylated Regions) Between Groups
methylation
DMR
updated 6 days ago by
Basti
★ 1.7k • written 7 days ago by
epianalysis
• 0
0
votes
1
reply
152
views
Download an example of fully-imputed VCF files ?
VCF
impute
updated 6 days ago by
barslmn
★ 1.9k • written 7 days ago by
Ben
• 0
0
votes
0
replies
124
views
How to annotate BED for tissue expression?
BED
annotation
7 days ago by
Fabio_bie88
• 0
0
votes
1
reply
1.7k
views
error when running local chlorop1.1
chlorop
updated 7 days ago by
Ram
40k • written 7.3 years ago by
gerrychang1985
▴ 40
0
votes
6
replies
288
views
gene correlations in between two groups
gene-expression
correlation
6 days ago by
biology_inform
▴ 40
0
votes
1
reply
127
views
Running Ibex on both BCR chains?
BCR
ibex
single-cell
updated 7 days ago by
Ram
40k • written 7 days ago by
actinia94
• 0
2
votes
9
replies
499
views
Calculation of TMB on gene level
genomics
updated 6 days ago by
svp
▴ 590 • written 7 days ago by
smrutimayipanda
▴ 20
0
votes
0
replies
118
views
Spike-in normalization in ATAC-Seq with DiffBind
ATAC-Seq
spike-in
DiffBind
7 days ago by
frueher
• 0
1
vote
2
replies
165
views
Is it normal if regress out the cell cycle effects but the DEGs are quite the similar (no big changes)
regress_out
Cell_cycle
Seurat
7 days ago by
alwayshope
▴ 30
0
votes
0
replies
118
views
filtering variants in a Strelka2 VCF file based on AD and AF
Filter
VCF
7 days ago by
Bogdan
★ 1.4k
2
votes
4
replies
280
views
Integrated Seurat object change name of the two conditions
R
integrated
Seurat
7 days ago by
camillab.
▴ 140
1
vote
5
replies
303
views
Enrichment of mitochondrial and ribosomal pathways - an artifact?
Pathway
Mitochondria
Ribosome
scRNA-seq
Enrichment
6 days ago by
omer.shomrat
• 0
0
votes
1
reply
212
views
Merging the filename with tsv files for master file
genomics
updated 6 days ago by
barslmn
★ 1.9k • written 9 days ago by
smrutimayipanda
▴ 20
4
votes
6
replies
386
views
Hisat2 index and alignment question
ubuntu
rna-seq
index
hisat2
updated 5 days ago by
Istvan Albert
98k • written 9 days ago by
Athena
• 0
0
votes
0
replies
157
views
My rMATS output file is not showing gene symbols and only XLOC gene ID are being shown.
rMATS
rMATS-turbo
9 days ago by
Nipan
• 0
2
votes
4
replies
315
views
Using STAR aligner to build index of hg38
hg38
STAR
alignment
RNAseq
index
8 days ago by
Grace
• 0
0
votes
0
replies
163
views
Convert RNASeq V2 data in cBioPortal having RSEM normalized results to TPM values
bioinformatics
TPM
RNASeqV2
cbioportal
RSEM
updated 9 days ago by
4galaxy77
2.8k • written 9 days ago by
shakyaram079
• 0
0
votes
2
replies
281
views
Modify the code to take most abundant reads from a cluster and process it.
cd-hit-est
clustering
9 days ago by
Mo
▴ 40
1
vote
2
replies
264
views
Understanding TCGA barcodes with dot in the fieldname
tcga
updated 7 days ago by
Zhenyu Zhang
▴ 980 • written 10 days ago by
Tahsin
• 0
0
votes
0
replies
164
views
Compare peaks between clusters in sc-ATAC
Peak-Calling
Epigenome
scATAC-seq
updated 10 days ago by
Ram
40k • written 10 days ago by
Ahmed.waraky
▴ 10
2
votes
7
replies
689
views
Weirdness in annotation (missing allele frequencies)
allele-frequency
gnomad
annovar
updated 11 hours ago by
Ram
40k • written 10 days ago by
Can Abdullah
• 0
1
vote
3
replies
245
views
Finding sequences in unannotated genomes using reference coordinates
consensus
VCF
updated 10 days ago by
Ram
40k • written 10 days ago by
Prangan
▴ 20
0
votes
1
reply
222
views
High amount of intronic/intergenic reads in SMARTer stranded total bulk RNAseq
RNA-seq
DNA
SMARTer
updated 10 days ago by
Ram
40k • written 10 days ago by
Mat
▴ 60
0
votes
0
replies
352
views
Why dbConnect GEOmetadb_demo.sqlite shows some information while dbConnect GEOmetadb.sqlite shows no information
R
GEO
GEOmetadb
updated 10 days ago by
Pierre Lindenbaum
157k • written 10 days ago by
nonaddldy
▴ 10
2
votes
5
replies
386
views
ATAC-seq troubleshoot - Just Noise
ATAC-seq
updated 6 days ago by
ATpoint
77k • written 10 days ago by
vk
▴ 40
0
votes
0
replies
178
views
High pvalues when using clusterProfiler for seurat
clusterProfiler
11 days ago by
Ahmed
• 0
5
votes
7
replies
447
views
Salmon index not progressing
salmon
updated 10 days ago by
Michael
53k • written 11 days ago by
camillab.
▴ 140
0
votes
0
replies
170
views
scanpy problem for empty cells
scanpy
updated 11 days ago by
Ram
40k • written 11 days ago by
Andy
▴ 90
0
votes
0
replies
174
views
Current landscape of approaches to scRNA-seq with nanopore sequencers?
scRNA-seq
nanopore
single-cell
8 days ago by
LauferVA
3.7k
0
votes
1
reply
219
views
How to download genomes and proteins from JGI in bulk via the command line?
jgi
cli
updated 11 days ago by
Ram
40k • written 11 days ago by
O.rka
▴ 680
0
votes
5
replies
470
views
genome data downloads for various strains
gisaid
hass-marr
updated 7 days ago by
Ram
40k • written 11 days ago by
iftikharmaryam123
• 0
0
votes
0
replies
188
views
Illumina methylation EPIC V2 array
ewas
methylation
enrichment
missMethyl
EPIC
11 days ago by
juliviglino
• 0
0
votes
1
reply
213
views
Esearch, Epost, and Efetch for Large Datasets in Biopython
eutils
biopython
entrez
updated 11 days ago by
Ram
40k • written 12 days ago by
Salem
• 0
3
votes
10
replies
542
views
STAR index not working
STAR
RNA-Seq
11 days ago by
camillab.
▴ 140
2
votes
3
replies
299
views
Can I infer the fraction of replicating cells from bulk RNA-seq data?
replication
apoptosis
updated 12 days ago by
Ram
40k • written 12 days ago by
txema.heredia
▴ 80
0
votes
1
reply
177
views
Prank checkpoint and restore?
prank
alignment
updated 12 days ago by
Ram
40k • written 12 days ago by
Pit
• 0
0
votes
13
replies
604
views
STAR Intron Motif Script Gives Segmentation fault Error
STAR
Linux
9 days ago by
Y
• 0
1
vote
3
replies
256
views
Sub-sampleing bam files based on sequencing_summary.txt (guppy output)
samtools
guppy
updated 12 days ago by
GenoMax
134k • written 12 days ago by
anika.john
• 0
0
votes
0
replies
123
views
Any way to predict hormone biosynthesis pathways in understudied organisms?
genomics
orthologs
biosynthesis-pathway
updated 12 days ago by
Ram
40k • written 12 days ago by
Mark
• 0
2
votes
4
replies
334
views
Refseq annotation | Populus trichocarpa
R
RNA-seq
plants
Refseq
updated 12 days ago by
Ram
40k • written 12 days ago by
P
• 0
1
vote
1
reply
208
views
Read Counts from BAM file
bam
samtools
qualimap
readcount
updated 12 days ago by
ATpoint
77k • written 12 days ago by
Smriti
▴ 10
0
votes
2
replies
246
views
Is it okay to just average the log2FC values across different cell types in pseudobulk scRNA-seq data?
scrna-seq
rna-seq
log2fc
12 days ago by
JLee
• 0
0
votes
1
reply
197
views
Identify parent of each read in a GAF
gaf
vgteam
vg
updated 12 days ago by
Jordan M Eizenga
▴ 360 • written 13 days ago by
cfourps
▴ 10
0
votes
3
replies
273
views
Errors while trying to run Scenic
Scenic
updated 13 days ago by
GenoMax
134k • written 13 days ago by
Stavroula
• 0
0
votes
3
replies
285
views
How to find target genes From RNA seq data?
Cotton
DGE
RNA-seq
updated 13 days ago by
Ram
40k • written 13 days ago by
Fizzah
▴ 30
0
votes
0
replies
142
views
maftools - median survival time doesn't match plot
R
survival
maftools
K-M
13 days ago by
BioGuy
• 0
1,000 results • Page
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Comment: How to convert data in r?
C: Convert list of Accession Numbers to Full Taxonomy
Convert list of Accession Numbers to Full Taxonomy
A: FASTA file with taxid, want to obtain lineage and put into header
Comment: How to convert data in r?
Answer: The RNA velocity are opposite in 'dynamical' and 'stochastic' modes.
Answer: The RNA velocity are opposite in 'dynamical' and 'stochastic' modes.
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Answer: How to assign gene names after kallisto when I add GFP?
by
dsull
★ 4.8k
kallisto relies on a transcriptome index, so simply add the GFP sequence to the transcriptome FASTA file and index it. The name in the outp…
Comment: How to convert data in r?
by
Ram
40k
While `%>%` is indeed exported by magrittr, `dplyr` is the most popular package to use them. dplyr imports magrittr, it does not define the…
Comment: Why should ATAC-seq mapped reads be shifted +4 and -5 for +strand and -strand, r
by
suragnair
• 0
So long as one's pipeline is consistent throughout, the shift is +4/-4 regardless of 1-based or 0-based indexing. But you're right that shi…
Comment: Why should ATAC-seq mapped reads be shifted +4 and -5 for +strand and -strand, r
by
suragnair
• 0
Each fragment is two cuts (one on + and - strands), which you capture if you do paired-end sequence. Conversely, each cut gives rise to two…
Comment: How to convert data in r?
by
kalavattam
▴ 190
Ah, thanks for pointing that out. `%>%` is made available with `magrittr`—are pipes in the `dplyr` namespace too? I wasn't aware of that. …
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LChart
3.4k
This question doesn't fit in nicely with the other set of questions (which tend to focus more on omics). However this sounds an awful lot l…
Answer: GOSt Plot
by
bk11
★ 1.3k
> How do I change the point size on the plot? I know how to do this in > ggplot, but can't find a command here to do this, even though the…
Answer: The RNA velocity are opposite in 'dynamical' and 'stochastic' modes.
by
james.hawley
▴ 40
Conflicting directions in RNA velocity analysis is not uncommon. [Gorin _et al._, 2022](https://journals.plos.org/ploscompbiol/article?id=…
Comment: counting from a single BAM file with multiple samples
by
LChart
3.4k
I think you can use UMI-tools to do this with something like `--extract_method=regex --bc-pattern=(?P<cell_1>8)$ --ignore-umi`. However I d…
Comment: counting from a single BAM file with multiple samples
by
bk11
★ 1.3k
You can do sth like that has been discussed [here][1]. [1]: https://www.10xgenomics.com/resources/analysis-guides/tutorial-navigating-…
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by
dr-device
• 0
Yes, its from a plate-based protocol but with literally only 20 cells. Im therefore planning on just using edgeR for my analysis rather tha…
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by
bk11
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Sure thing! If it worked, please accept the answer.
Comment: Help with error running velocyto
by
Chris
▴ 200
The tutorial doesn't mention we need to load `samtools`. Thank you so much! You are awesome. It is running now.
Comment: How to extract the first and last 2 nt from multiple sequences in a FASTA file?
by
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40k
Accept this answer to mark the post as solved, and please don't ask the same question again.
Comment: extract first and last n bp from fasta file from multiple fasta file in R
by
Ram
40k
OP asked [the exact same question 2 years ago][1] and got the seqkit subseq answer from shenwei himself. Really odd behavior. [1]: https…
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