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1,000 results • Page
1 of 20
Sort: Rank
Rank
Views
Votes
Replies
0
votes
2
replies
22
views
bam merging for archaic samples
samtools
bam
ancient
merge
8 minutes ago by
Matteo Ungaro
▴ 100
1
vote
0
replies
126
views
The majority of the sequences in Trinity ID do not start from start codons. Can the sequences still be used for primer design and validation?
Primers
TRINITY
6 hours ago by
mathavanbioinfo
▴ 90
0
votes
0
replies
114
views
Using samtools with GCS (google cloud storage) on docker a docker container seems to give "Protocol Not Supported" error
samtools
gcs
16 hours ago by
abhishekghadge
• 0
0
votes
3
replies
311
views
how to find genes that are significantly expressed in brain cortex samples
rna-seq
gene-expression
updated 10 hours ago by
Ram
43k • written 16 hours ago by
rheab1230
▴ 140
1
vote
4
replies
403
views
what's the genomic location and name of gene responsible for orange and black patches in calico cats
genomes
updated 16 hours ago by
Matthias Zepper
4.5k • written 18 hours ago by
Ann
★ 2.4k
0
votes
0
replies
101
views
agilent microarray gpl data does not have gene symbol column
microarray
agilent
gpl
updated 17 hours ago by
Ram
43k • written 19 hours ago by
mja
• 0
0
votes
0
replies
104
views
scRNA Cluster frequency
CV
PCA
scRNA
Cluster-Frequency
19 hours ago by
Nitin
• 0
0
votes
0
replies
110
views
absolute path for symbolic links in Snakefile
Snakemake
updated 19 hours ago by
Ram
43k • written 20 hours ago by
yifangt86
▴ 60
0
votes
0
replies
100
views
Chemical structure validation
structure
cap
validation
metabolite
20 hours ago by
Rodolfo Adrián
• 0
0
votes
1
reply
172
views
Downloading the raw microarray data from GEO
.gpr
microarray
.CEL
updated 20 hours ago by
GenoMax
141k • written 20 hours ago by
bioyas
▴ 10
0
votes
0
replies
119
views
Copy number variation plot
Copy-number-variation
genomics
updated 21 hours ago by
Ram
43k • written 21 hours ago by
Emmi
• 0
0
votes
0
replies
102
views
SNP calling with ANGSD and ngsLD. How many SNPs?
lcwgs
ngsTools
ANGSD
genomics
22 hours ago by
DanielEB_fisk
▴ 20
0
votes
1
reply
198
views
Could you please assist in identifying this cluster?
single-cell
updated 21 hours ago by
Ram
43k • written 23 hours ago by
Kazo
• 0
0
votes
0
replies
123
views
Conversion to tree format
PLINK
bed
Figtree
SplitsTree
updated 21 hours ago by
Ram
43k • written 1 day ago by
Dinmukhamed
• 0
0
votes
0
replies
124
views
miRDeep2: How to get the read counts
mirdeep2
1 day ago by
Atul K.
• 0
0
votes
0
replies
131
views
Lncipedia GTF file error
Proteomics
updated 21 hours ago by
Ram
43k • written 1 day ago by
atharvakarkare14
▴ 10
3
votes
3
replies
352
views
Free/open source 23andme-like analysis
SNPs
genomics
NGS
VCF
updated 5 hours ago by
swbarnes2
14k • written 1 day ago by
joe
▴ 510
0
votes
2
replies
437
views
How to get the reference panel for UKBB
UKBB
GWAS
clump
1 day ago by
航太郎
• 0
0
votes
0
replies
143
views
create genewise sync file in popoolation
popoolation
updated 1 day ago by
GenoMax
141k • written 1 day ago by
N.Y.Wiyana-Hewage
• 0
0
votes
2
replies
302
views
python file for coding potential calculator
cpc2.py
1 day ago by
Ashok
• 0
0
votes
2
replies
294
views
error in coding potential calculator CPC2
cpc
CPC2
coding-potential
1 day ago by
Ashok
• 0
0
votes
2
replies
329
views
miRNAs quantification using mirdeep2 tool
differential-expression
mirdeep2
miRNA
1 day ago by
Ashok
• 0
0
votes
0
replies
148
views
Variant Generation Using Longshot
long-reads
variant-calling
ONT
minion
longshot
updated 1 day ago by
Ram
43k • written 1 day ago by
samuelkalandarov2002
• 0
0
votes
2
replies
349
views
QC exclusion of A/T or G/C alleles to avoid strand issues
quality-control
SNV
QC
SNP
21 hours ago by
_quantum_girl_
▴ 10
0
votes
0
replies
153
views
traying to make a maf from an already annotated vcf
vcf2maf
VEP
maftools
updated 1 day ago by
Ram
43k • written 1 day ago by
Javier
• 0
0
votes
0
replies
149
views
Pruning with plink finds a majority of SNPs in very high LD
LD
SNP
plink
pruning
1 day ago by
enferdeflame
• 0
0
votes
0
replies
162
views
Difference when using difference number of pathways score to compare between 3 groups
GSVA
Limma
1 day ago by
Chris
▴ 260
0
votes
1
reply
393
views
How to add rowLinks, rowTree in SummarizedExperiment manually.
phylogenetic-tree
microbiome
updated 1 day ago by
Ram
43k • written 19 months ago by
Muhammad
• 0
0
votes
0
replies
147
views
How can I obtain the tissue or sample name alongside the Tau score in tspex?
tau-score
tspex
updated 1 day ago by
Ram
43k • written 2 days ago by
bioinfo223
▴ 10
0
votes
0
replies
160
views
How to root an unrooted tree with a known root tree?
phylogenetics
rooting
R
2 days ago by
P.
• 0
0
votes
0
replies
153
views
Issues with SnpEff Assuming Circular Chromosomes in Eukaryotic Genome Analysis
snpEff
2 days ago by
ekirsch
• 0
1
vote
1
reply
339
views
Load a full GFF3 into annotation track using arrow (Apollo)
arrow
python-apollo
Apollo
20 hours ago by
renan.igor
• 0
0
votes
0
replies
152
views
filter the most significant ligand and receptors in netVisual_bubble in cell chat
cellchat
2 days ago by
synat.keam
▴ 100
0
votes
1
reply
242
views
assembly using CCS, CLR, CCS_CLR sequences together?
assembly
updated 2 days ago by
GenoMax
141k • written 2 days ago by
ycts
• 0
0
votes
4
replies
543
views
Per base sequence content failed miserably
fastqc
sequence
trimmomatic
NGS
assembly
1 day ago by
Kai Xin
• 0
1
vote
1
reply
207
views
Retrieve a % coverage for each transcript
RNA-seq
updated 2 days ago by
Ram
43k • written 2 days ago by
jammydodger123456
▴ 40
0
votes
2
replies
334
views
How can I solve this error?
metal
2 days ago by
22211020193
• 0
0
votes
0
replies
157
views
Struggling to lift genome builds
PLINK
liftover
GWAS
updated 2 days ago by
GenoMax
141k • written 2 days ago by
srimmer
• 0
1
vote
2
replies
244
views
Can I readmap short reads to rDNA references?
rDNA
assembly
updated 2 days ago by
Ram
43k • written 2 days ago by
aniigodwinn
• 0
0
votes
0
replies
310
views
Looking for an explanation as to what the Peak Score is from HOMER, not finding any answers on Biostars yet
Homer
ATAC-seq
3 days ago by
Ronin
• 0
2
votes
7
replies
742
views
Removing duplicates
duplicates
ONT
minimap2
updated 1 day ago by
joe
▴ 510 • written 3 days ago by
quentinperriere
• 0
0
votes
1
reply
175
views
Annotating a dataset with gnomAD v4 using Hail
Python
Sequencing
Hail
Exome
Annotation
updated 3 days ago by
DKA
▴ 40 • written 3 days ago by
james.melhorn
• 0
0
votes
1
reply
164
views
Freyja plot error
Freyja
updated 2 days ago by
Ram
43k • written 3 days ago by
Adyasha
• 0
0
votes
0
replies
142
views
BIOM format can contain tabular data ?
BIOM
phyloseq
biomformat
R
3 days ago by
chemokine-1
▴ 10
0
votes
4
replies
452
views
How to reduce the variation of CIBERSORTx results?
CIBERSORTx
deconcolution
2 days ago by
feather-W
• 0
0
votes
2
replies
189
views
Bedmethyl file format
bedmethyl
methylation
updated 3 days ago by
GenoMax
141k • written 3 days ago by
njornet
▴ 20
0
votes
0
replies
158
views
sequence table for DADA2 chimera filtering
OTU
sequencetable
DADA2
3 days ago by
Ali
• 0
2
votes
4
replies
285
views
SnpEff annotates coding duplication as intronic?
snpeff
3 days ago by
kirill.zaslavsky
• 0
0
votes
2
replies
219
views
Calculating percentage of cells proliferating in single cell rna seq data
statistics
scRNA-seq
proliferation
3 days ago by
mropri
▴ 150
0
votes
0
replies
133
views
Input into WGCNA and Error on constructNetworks on RNA-Seq data - unique rownames
rownames
RNA-Seq
WGCNA
unique
3 days ago by
shasabhi1
• 0
1,000 results • Page
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The majority of the sequences in Trinity ID do not start from start codons. Can the sequences still be used for primer design and validation?
Answer: Cellranger count error: Input FASTQ file ended prematurely
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Recent Replies
Comment: bam merging for archaic samples
by
Matteo Ungaro
▴ 100
@Pierre Lindenbaum I see. So, I can actually manipulate just one single RG tag at the time with `samtools`? Which flag should I use, or sho…
Comment: bam merging for archaic samples
by
Pierre Lindenbaum
161k
RG tag "L9302" is missing the the BAM header. You should have something like this in the BAM header ``` @RG ID:L9302 SM:L9302 ``` may be…
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161k
> . Is there any way to find through sam or bam file that which chromosomal position are maximum reads aligning to? samtools depth i…
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by
BioinfGuru
★ 1.7k
That makes a lot more sense. Thank you.
Answer: Removing Illumina microarray probes with no expression HumanHT-12v4
by
Gordon Smyth
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Type ``` help("read.idat") ``` and read the example at the bottom of the page, which shows you how to set the `other$Detection` component o…
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Accurately assessing a few QC metrics is trivially easy. Assessing the significance of variants in a clinically responsible way is really…
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If _1 and _2 really are the same library, you should rename the fastqs, or make symlinks, such that they have the same sample name, but dif…
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by
ATpoint
81k
Just delete the index read files. They just take up space and will never be used.
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by
swbarnes2
14k
Yes. Why wouldn't you use, say, AddOrReplaceReadsGroups?
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Thank you. Decreased the threads to 2 (changed the threads: 2 in the config file; but it was somehow using 4). It did run for sometime but …
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Comment: how to find genes that are significantly expressed in brain cortex samples
by
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43k
Can you explain the normalization or give me a link that describes it, please? Comparison across samples will always be caveated as RNA-seq…
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Sorry, I see how harsh I come across. It's not good practice to use multiple versions of R in the same project. I can understand legacy pro…
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> I have ensured that I am using the latest version of MEGA and that other applications are not consuming excessive resources during the an…
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