0
votes
10
replies
349
views
2
votes
9
replies
784
views
0
votes
8
replies
274
views
2
votes
7
replies
805
views
updated 6 days ago by
joe
▴
510
•
written 7 days ago by
quentinperriere
•
0
0
votes
6
replies
423
views
1
vote
5
replies
237
views
updated 2 days ago by
Ram
43k
•
written 2 days ago by
marco.barr
▴
80
2
votes
5
replies
674
views
0
votes
5
replies
5.9k
views
updated 4 days ago by
ndm1096
•
0
•
written 6.5 years ago by
ac.research
▴
30
0
votes
5
replies
259
views
2
votes
5
replies
426
views
2
votes
4
replies
236
views
0
votes
4
replies
572
views
3
votes
4
replies
3.4k
views
0
votes
4
replies
491
views
6 days ago by
feather-W
•
0
2
votes
4
replies
1.4k
views
2
votes
3
replies
3.0k
views
updated 6 days ago by
Ram
43k
•
written 6.9 years ago by
endikavarela
•
0
1
vote
3
replies
4.1k
views
1
vote
3
replies
225
views
23 hours ago by
Art1ess
•
0
1
vote
3
replies
2.4k
views
updated 2 days ago by
chrchang523
10k
•
written 6.1 years ago by
Ginevra
▴
10
3
votes
3
replies
461
views
0
votes
3
replies
3.1k
views
updated 6 days ago by
Ram
43k
•
written 9.0 years ago by
siddharth.patel.153
•
0
1
vote
3
replies
189
views
0
votes
2
replies
2.8k
views
updated 6 days ago by
Ram
43k
•
written 7.0 years ago by
endikavarela
•
0
0
votes
2
replies
370
views
5 days ago by
Ashok
•
0
0
votes
2
replies
531
views
0
votes
2
replies
536
views
0
votes
2
replies
311
views
6 days ago by
Sudip
•
0
1
vote
2
replies
1.9k
views
updated 6 days ago by
Ram
43k
•
written 6.5 years ago by
Alternative
▴
270
0
votes
2
replies
409
views
5 days ago by
Ashok
•
0
0
votes
2
replies
192
views
updated 5 hours ago by
Ram
43k
•
written 1 day ago by
v.berriosfarias
▴
140
0
votes
2
replies
176
views
0
votes
2
replies
136
views
1
vote
2
replies
226
views
updated 2 days ago by
Gordon Smyth
★
7.0k
•
written 16 days ago by
Netanel
•
0
0
votes
2
replies
417
views
5 days ago by
_quantum_girl_
▴
10
0
votes
2
replies
251
views
updated 3 days ago by
Ram
43k
•
written 4 days ago by
Matteo Ungaro
▴
100
0
votes
2
replies
365
views
6 days ago by
22211020193
•
0
0
votes
1
reply
398
views
0
votes
1
reply
109
views
updated 21 hours ago by
Mensur Dlakic
★
27k
•
written 1 day ago by
benguyarenbeyaz98
•
0
1
vote
1
reply
151
views
0
votes
1
reply
119
views
updated 1 day ago by
bk11
★
2.3k
•
written 1 day ago by
Susmita Mandal
▴
110
0
votes
1
reply
817
views
0
votes
1
reply
258
views
0
votes
1
reply
1.8k
views
updated 6 days ago by
Ram
43k
•
written 6.6 years ago by
boymin2020
▴
80
0
votes
1
reply
135
views
0
votes
1
reply
123
views
updated 1 day ago by
bk11
★
2.3k
•
written 1 day ago by
alphaflylizard
•
0
1
vote
1
reply
388
views
0
votes
1
reply
120
views
0
votes
1
reply
139
views
0
votes
1
reply
114
views
0
votes
1
reply
278
views
Recent Votes
Recent Locations •
All
United States, 1 minute ago
South Korea, 4 minutes ago
Athens, 6 minutes ago
United States, 7 minutes ago
Poland, 9 minutes ago
Ambler, 12 minutes ago
California, USA, 21 minutes ago
Recent Awards •
All
Popular Question to
Simone
▴
10
Popular Question to
carlopecoraro2
★
2.5k
Popular Question to
daffodil
▴
10
Popular Question to
Russ
▴
500
Popular Question to
camillab.
▴
160
Popular Question to
pramach1
▴
40
Popular Question to
Adyasha
•
0
Recent Replies
Answer: clustalo - align milions of short seq, memory issue
by
emmanouil.a
▴
120
Hi GenoMax and Mensur Dlakic, thank you for your feedback. I'm changing approach in the way to work with a smaller number of seq.
Comment: Annovar using R package
by
Pierre Lindenbaum
161k
duplicate of your previous question: https://www.biostars.org/p/9592646/
why using R when there are shell-based tools like VEP ? slivar , …
Comment: Trouble with Pilon installation
by
Lissa Cruz Saavedra
•
0
Hi
I am trying to install the software but I didn't find the way to clone from github. Could you help me with the pipeline of instruction…
Comment: Adding CB tag to bam file
by
swbarnes2
14k
I think both these programs are just adding the same tag to every line. I don't think either cares whether it's called RG or CB
Comment: PCA plot (Suggestions Needed)
by
swbarnes2
14k
Even if this was real data, the tiny numbers for the PC1 and PC2 indicate that the differences between the samples are all absolutely tiny.…
Comment: Creating Synthetic Sequences for a ML Model
by
biochugs
•
0
Thanks for the input. I'll continue to think of more ideas that may be achievable.
Comment: PCA plot (Suggestions Needed)
by
Jeremy
▴
890
You could also try kernel PCA, which is a non-linear form of PCA, although this will be more time-consuming.
Comment: Troubleshooting RNA-seq data with DNA contamination
by
DKA
▴
40
I have unstranded RNA-seq data. Please share the tool with me, if you do not mind.
Comment: PCA plot (Suggestions Needed)
by
Mensur Dlakic
★
27k
Everything that @f1f58aed said, plus that there may be no data manipulation that will give you the clusters you expected. Sometimes there a…
Answer: Creating Synthetic Sequences for a ML Model
by
Mensur Dlakic
★
27k
It is a good idea, though I don't see how it would work in practice.
I think you will encounter at least one additional problem on top of …
Answer: PCA plot (Suggestions Needed)
by
Jeremy
▴
890
Right now, the WT samples are clustering together, but you have one mutant sample off by itself, which could be an outlier. How did you sc…
Comment: Adding CB tag to bam file
by
GenoMax
141k
@yokofakun had written this: https://www.biostars.org/p/336965/
https://github.com/ruqianl/appendCB may also be useful.
Comment: Troubleshooting RNA-seq data with DNA contamination
by
GenoMax
141k
Please post information about your tool as a `tools` category post. Don't wait/ask other users to ask you.
Comment: Adding CB tag to bam file
by
Maria
•
0
Sorry for the delay in my answer, I got sidetracked.
I am using
```samtools addreplacerg -r "@RG\tID:$cell" -o $cell.bam -h $LINE```
bu…
Answer: Troubleshooting RNA-seq data with DNA contamination
by
haibol2017
▴
10
We just developed a R package to tackle this problem.
Do you have stranded or unstranded RNA-seq data contaminated with gDNA?
If you wou…
Traffic: 1999 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6