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522 results • Page
2 of 11
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Votes
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0
votes
5
replies
486
views
seg fault, core dumped with manta 1.6.0
manta
gdb
updated 10 days ago by
Ram
43k • written 14 days ago by
Greg
• 0
0
votes
5
replies
313
views
BUSCO analysis failed due to Duplicate of sequence in Input genome fasta. How to solve this problem ?
duplicate
BUSCO.
updated 14 days ago by
GenoMax
141k • written 14 days ago by
Sony
▴ 10
2
votes
5
replies
1.6k
views
Adding functional annotation and meta-data of MAKER/BRAKER GFF
metadata
functional-annotation
BRAKER
MAKER
updated 17 days ago by
andorjkiss
▴ 40 • written 15 months ago by
javanokendo
▴ 60
0
votes
5
replies
475
views
How to assure that sequence consensus is genuine?
genome
12 days ago by
marongiu.luigi
▴ 710
0
votes
5
replies
312
views
Incongruencies in GT assignment by Freebayes
depth
freebayes
heterozygous
genotype
13 days ago by
virginia.baraja
• 0
5
votes
5
replies
7.2k
views
Converting scRNA-Seq to Bulk-RNASeq
scRNASeq
bulk-RNASeq
RNA-Seq
single-cell
updated 8 days ago by
veldahan86
• 0 • written 3.8 years ago by
Assa Yeroslaviz
★ 1.8k
0
votes
5
replies
402
views
Error running local blastn in R using system2
blastn
NCBI
R
20 days ago by
Harrison
• 0
0
votes
5
replies
1.2k
views
RNAseq differential gene expression analysis yield results which come from digestive content
differential-gene-expression
RNA-Seq
updated 9 days ago by
Ram
43k • written 6.4 years ago by
pablo61991
▴ 90
0
votes
5
replies
462
views
Deeptools scale issue for plotHeatmap
deeptools
computeMatrix
plotHeatmap
27 days ago by
Learner
• 0
0
votes
5
replies
372
views
Used featureCounts to generate a count matrix for sc RNA-seq and it has all 0s.
scRNA-seq
updated 8 days ago by
GenoMax
141k • written 9 days ago by
Shay
• 0
1
vote
5
replies
324
views
samtools filtering
samtools
updated 8 days ago by
jkbonfield
★ 1.2k • written 15 days ago by
sansan_96
▴ 80
0
votes
5
replies
1.8k
views
Demultiplexing multiple amplicons from one fastq based on primer sequence
demultiplexing
illumina
updated 10 days ago by
Arup Ghosh
3.2k • written 3.4 years ago by
WhiteN8
• 0
2
votes
5
replies
346
views
Where to find the homopolymer regions bed file for Hg002 genome?
simple-repeats
hg002
bed
homopolymer-regions
2 days ago by
Bikram Kumar
• 0
0
votes
5
replies
361
views
Use of Kraken output for functional analysis
metagenomics
kraken
shotgun
15 days ago by
Srinka
▴ 20
0
votes
4
replies
256
views
Adaptive sampling for whole chromosomes
T2T
Nanopore
AdaptiveSampling
aneuploidy
centromeres
8 days ago by
njornet
▴ 20
0
votes
4
replies
288
views
Questions about transfering wgs file to reference genome file
fasta
cram
genome
7 days ago by
me
• 0
1
vote
4
replies
785
views
I want to calculate if the sample has whole genome duplication event or not?
sequencing
wgs
9 days ago by
Hyper_Odin
▴ 310
2
votes
4
replies
272
views
Addmetadata to seurat obj
seurat
8 days ago by
synat.keam
▴ 100
0
votes
4
replies
420
views
How to reduce the variation of CIBERSORTx results?
CIBERSORTx
deconcolution
1 day ago by
feather-W
• 0
1
vote
4
replies
2.8k
views
removing confounding batch effect with RUVseq
RUVseq
RNA-Seq
batch-effect
DESeq2
updated 1 day ago by
Ram
43k • written 5.1 years ago by
grant.hovhannisyan
★ 2.6k
3
votes
4
replies
3.4k
views
any recommended tools for batch effect and normalization not written in R?
R
RNA-Seq
combat
python
batch-effect
updated 17 hours ago by
Ram
43k • written 7.1 years ago by
dyollluap
▴ 310
3
votes
4
replies
334
views
Single cell analysis with "meta".tsv.gz file
Single-cell
16 days ago by
Estevão
• 0
4
votes
4
replies
348
views
Average expression of a sample in single-cell data
single-cell
rna-seq
16 days ago by
newuser2024
• 0
2
votes
4
replies
272
views
SnpEff annotates coding duplication as intronic?
snpeff
2 days ago by
kirill.zaslavsky
• 0
0
votes
4
replies
304
views
Coverage observed in introns in the Knockdown genes but not in knockout genes
RNA-seq
STAR
IGV
22 days ago by
rohitsatyam102
▴ 840
2
votes
4
replies
1.4k
views
How to fix Error in dat[, batch == batch_level] : (subscript) logical subscript too long when removing batch effects
microarray
combat
batch-effect
median-normalization
updated 17 hours ago by
Ram
43k • written 17 months ago by
Dominique
• 0
0
votes
4
replies
389
views
Is it reasonable to discard reads that show variation of quality across its length?
Trimming
21 days ago by
BRENO
• 0
0
votes
4
replies
1.8k
views
Correcting batch effect in unevenly distributed groups
RNA-Seq
batch-effect
R
updated 1 day ago by
Ram
43k • written 4.8 years ago by
jperezboza
▴ 20
0
votes
4
replies
1.1k
views
Getting biological replicates from SRA run NCBI
fastq
AWS
SRA
RNAseq
17 days ago by
Andres
▴ 20
0
votes
4
replies
456
views
Per base sequence content failed miserably
fastqc
sequence
trimmomatic
NGS
assembly
5 hours ago by
Kai Xin
• 0
0
votes
4
replies
318
views
Compute bootstrap values on consensus tree
phylogeny
tree
raxml
3 days ago by
pablo
▴ 300
0
votes
4
replies
320
views
What is the samtools flag used in bamPEFragment?
deeptools
updated 7 days ago by
GenoMax
141k • written 10 days ago by
Soohyun
• 0
2
votes
4
replies
2.6k
views
DESeq2 inconsistent results
differential-gene-expression
RNA-Seq
DESeq2
updated 9 days ago by
Ram
43k • written 8.0 years ago by
Chris Gene
▴ 80
2
votes
4
replies
295
views
Number of CPUs to use by DRAP for transcriptomic assmbly
DRAP
CPU
transcriptomics
20 days ago by
Mohamed Abderrahmane
▴ 10
0
votes
4
replies
2.7k
views
MACS2 BAMPE and BEDPE gave dramatically different "mean fragment size"
ChIP-Seq
updated 25 days ago by
axol0tl
• 0 • written 6.5 years ago by
Xinwei Han
• 0
1
vote
4
replies
1.3k
views
Splitting long reads into shorter chunks
long-read
updated 13 days ago by
Ram
43k • written 5.8 years ago by
pennakiza
▴ 60
1
vote
4
replies
270
views
Removing batch effect using combat-seq
batch
effect
RNA-Seq
combat-seq
updated 17 days ago by
BioinfGuru
★ 1.7k • written 18 days ago by
Netanel
• 0
1
vote
4
replies
2.2k
views
Remove batch effect for prediction of RNA-seq data
RNA-Seq
batch-effect
Random-Forest
updated 1 day ago by
Ram
43k • written 4.7 years ago by
Vlad
▴ 10
1
vote
4
replies
2.4k
views
Batch effect control in GWAS data.
Batch-effect
omniexpress
GWAS
GSA
updated 1 day ago by
Ram
43k • written 4.8 years ago by
saksis.rihards
▴ 10
1
vote
4
replies
373
views
what is the normal range of htseq-count output for homo sapiens RNA-Seq
Transcriptomics
htseq-count
RNA-Seq
NGS
hisat2
updated 6 days ago by
BioinfGuru
★ 1.7k • written 7 days ago by
Morteza
• 0
1
vote
4
replies
343
views
Molecular docking with Autodock
Docking
proteomics
updated 13 days ago by
Ram
43k • written 11 weeks ago by
shwetamgr1
▴ 10
2
votes
4
replies
375
views
Per Base Sequence Content - continuous raise of G%
fastqc
updated 8 days ago by
GenoMax
141k • written 9 days ago by
pl.terzian
• 0
1
vote
4
replies
338
views
How to get human-mouse conservation score from UCSC
conservation
mouse
IGV
human
score
13 days ago by
diqixiaoyaoer
▴ 10
0
votes
3
replies
440
views
How do I get the gene annotations as a text file from a genbank file?
sequence-annotation
genbank
updated 20 days ago by
Ram
43k • written 21 days ago by
eae6d2e7
• 0
0
votes
3
replies
327
views
Resources regarding Proteomics (In-Silico)
molecular-simulation
docking
protein
proteomics
updated 17 days ago by
Ram
43k • written 4 weeks ago by
Saurabh
• 0
0
votes
3
replies
257
views
rRNA depletion of RIP-seq samples
rRNA
RIP-seq
updated 9 days ago by
joe
▴ 510 • written 10 days ago by
CrisRisu
• 0
0
votes
3
replies
1.8k
views
How to remove batch effect in copy number segment mean
Combat
CNA
Batch-Effect
updated 1 day ago by
Ram
43k • written 5.5 years ago by
sugus
▴ 150
0
votes
3
replies
693
views
PCR duplicates in FFPE RNASeq
PCR-duplicates
RNA-Seq
FFPE
Variant-Calling
updated 23 days ago by
Ram
43k • written 21 months ago by
Gama313
▴ 120
1
vote
3
replies
341
views
how to do the repeatability filtering of vcf files?
snp
vcf.
updated 17 days ago by
Pierre Lindenbaum
161k • written 19 days ago by
Max
• 0
2
votes
3
replies
247
views
P-value Threshold Consideration in Multi-Sample RNA-Seq Experiment
statistics
RNA-Seq
p-value
updated 16 days ago by
Ram
43k • written 17 days ago by
Netanel
• 0
522 results • Page
2 of 11
Recent Votes
Answer: Plink merge errors.
Gene Set Enrichment Analysis
Gene Set Enrichment Analysis
A: Counting Number Of Bases In A Fastq File
Free/open source 23andme-like analysis
Comment: Free/open source 23andme-like analysis
Answer: Missing protein (VEGF-A) in String db
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i.sudbery
19k
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biwdpang
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Michael
54k
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Recent Replies
Answer: Plink merge errors.
by
Dinmukhamed
• 0
I found answer https://www.biostars.org/p/101191/.
Comment: Ciriquant not configuring hisat2 indexed files
by
Atul K.
• 0
it worked. Thank you. Now my terminal is crashing, seems 16gb of RAM and 4 cores isn't enough. Can you suggest something?
Comment: Adding CB tag to bam file
by
Maria
• 0
I checked Picard ```AddOrReplaceReadsGroups``` and ```AddOATag```. Is that what you're referring to?
Comment: Free/open source 23andme-like analysis
by
Michael
54k
Noted that 'accurate' wasn't among your criteria ;) Anyway, most variant callers are free open-source software. However, for ancestry ana…
Answer: Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (wi
by
Pierre Lindenbaum
161k
> server so I cannot use Blat for this purpose. blat is avilable as a standane tool: http://hgdownload.soe.ucsc.edu/admin/exe/linux.x86…
Answer: Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (wi
by
b.contreras.moreira
▴ 160
My favourite for this task would be [GMAP]( http://research-pub.gene.com/gmap ), which can produce GFF format with option `-f 2`. It is…
Answer: Prediction of Ribosomal RNA Genes Using RNAmmer Software
by
colindaven
6.3k
Another alternative is the truly free implementation barrnap https://github.com/tseemann/barrnap. This is also installable via conda so qui…
Comment: Filtration Of Reads With Length Lower Than 30 From Bam
by
Hagen
▴ 10
thanks! This saved me quite some time, I was about to write code to calculate the aligned length from the CIGAR. But this can be done with …
Comment: Per base sequence content failed miserably
by
Kai Xin
• 0
will look into that. Thank you very much
Comment: Korean human genome reference file
by
SeoGyun
• 0
Thanks..... But I want to do imputation, so I have to make it as a vcf file, but the site only has a fatsa file....
Comment: miRNAs quantification using mirdeep2 tool
by
Ashok
• 0
thank you for your reply https://github.com/rajewsky-lab/mirdeep2/blob/master/TUTORIAL.md i saw this github tutorial for miRNA quantifica…
Comment: Load a full GFF3 into annotation track using arrow (Apollo)
by
cmdcolin
★ 3.8k
Hi there, I let a developer know of this thread and they said they can check it out next week. Feel free to post to the https://github.com/…
Comment: How to get the reference panel for UKBB
by
航太郎
• 0
Thanks for your guide!
Comment: Removing duplicates
by
joe
▴ 510
I'd try those OP @samuel recommends My guess is you're new to ONT, fast5 is the 'raw data' which is current over time...other than generat…
Comment: RNA seq differential expression analysis
by
swbarnes2
14k
No. Find a tutorial, try it, then if you have specific questions, ask of the galaxy help site.
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