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327 results • Page
3 of 7
Sort: Views
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Views
Votes
Replies
1
vote
4
replies
329
views
Gene expression
RNA-seq
DESeq2
Heatmap
updated 27 days ago by
ATpoint
77k • written 27 days ago by
Ralph
• 0
0
votes
2
replies
327
views
Sentrix Array Genotyping
Illumina
25 days ago by
joo
• 0
1
vote
4
replies
326
views
Split reads along the genome in my samples
DNA
sequencing
20 days ago by
paulaotero.sanchez
• 0
1
vote
0
replies
324
views
Strand orientation and GC skew
CGView
strand-orientation
GC-skew
updated 12 days ago by
Ram
40k • written 13 months ago by
A_heath
▴ 140
1
vote
2
replies
322
views
Converting scMultiome data to loom using SEURAT
scMultiome
CCAF
Seurat
25 days ago by
halimaakhter014
• 0
1
vote
4
replies
321
views
rRNA filtering from human RNA-seq data
rRNA
RNA-seq
14 days ago by
deniz
▴ 10
2
votes
4
replies
317
views
Using STAR aligner to build index of hg38
hg38
STAR
alignment
RNAseq
index
9 days ago by
Grace
• 0
0
votes
1
reply
317
views
txt files in minfi
differential-methylation-analysis
updated 20 days ago by
Ram
40k • written 6 weeks ago by
ananta.kapoor
• 0
0
votes
2
replies
317
views
Sample size estimation with ssizeRNA in R
transcriptomics
power-analysis
RNA-seq
sample-size-estimation
27 days ago by
Nana
• 0
1
vote
3
replies
315
views
Requesting further clarification on interpreting relative gene expression strength
R
normalization
TPM
RNA-seq
DESeq2
updated 19 days ago by
ATpoint
77k • written 4 weeks ago by
Abhishek
• 0
0
votes
0
replies
314
views
Can plantiSMASH be used with a transcriptome instead of a genome?
plantiSMASH
updated 1 day ago by
Ram
40k • written 22 months ago by
kristina.mahan
▴ 160
0
votes
2
replies
312
views
NCBI Genome Workbench Sequence ID edit
NCBI
Workbench
Genome
Sequence
28 days ago by
Paige
• 0
0
votes
1
reply
310
views
how to determine n_cells_by_count
scanpy
updated 18 days ago by
yl759
▴ 40 • written 12 weeks ago by
dalibenam64
• 0
1
vote
5
replies
309
views
Enrichment of mitochondrial and ribosomal pathways - an artifact?
Pathway
Mitochondria
Ribosome
scRNA-seq
Enrichment
7 days ago by
omer.shomrat
• 0
2
votes
3
replies
309
views
Genewise output help
genewise
updated 14 days ago by
Jean-Karim Heriche
26k • written 15 days ago by
lorenzoedg
• 0
0
votes
2
replies
306
views
ClusterProfiler GeneRatio demoninator not the same for all values
enrichment-analysis
clusterprofiler
RNA-seq
deseq2
26 days ago by
Lily
• 0
0
votes
2
replies
302
views
Deseq2 colData for single condition
RNA-seq
17 days ago by
Petesview
• 0
1
vote
2
replies
302
views
functional analysis
16S
metabarcoding
functionalanalysis
1 day ago by
safeassli
• 0
0
votes
2
replies
299
views
miRNAseq - over-presented sequence in negative control
miRNAseq
adaptor
dimer
21 days ago by
MH85
• 0
2
votes
3
replies
299
views
Can I infer the fraction of replicating cells from bulk RNA-seq data?
replication
apoptosis
updated 13 days ago by
Ram
40k • written 13 days ago by
txema.heredia
▴ 80
1
vote
5
replies
299
views
CollectRnaSeqMetrics (Picard) output to convert FeatureCounts into TPM
R
Picard
CollectRnaSeqMetrics
updated 5 days ago by
Ram
40k • written 6 days ago by
camillab.
▴ 140
0
votes
3
replies
298
views
Extracting/assembling organellar reads from RNAseq data
transcriptome
star
alignment
mapping
updated 15 days ago by
LChart
3.4k • written 22 days ago by
Miles
• 0
0
votes
3
replies
297
views
Assigning GO ids to blast hits
Linux
GO
blast
updated 7 days ago by
gv
• 0 • written 27 days ago by
Mousumi Akter
• 0
0
votes
4
replies
297
views
Analyzing time-patterns in RNA Seq data only having results_apeglm
R
sequencing
20 days ago by
Paul
• 0
5
votes
3
replies
295
views
Visualisation of accessible regions in single cell atacseq data
visualization
scATAC-seq
single-cell
updated 19 days ago by
Ram
40k • written 19 days ago by
sarahmanderni
▴ 90
2
votes
2
replies
292
views
bedtools intersect by position & stand not working even with common regions
bedtools
genomic
intersect
bedops
intervals
1 day ago by
Alewa
▴ 140
1
vote
3
replies
292
views
Question regarding MACS2 approach of peak calling
MACS2
sequencing-depth
chipseq
updated 13 days ago by
ATpoint
77k • written 22 days ago by
rkc5
• 0
0
votes
6
replies
292
views
gene correlations in between two groups
gene-expression
correlation
7 days ago by
biology_inform
▴ 40
0
votes
3
replies
289
views
How to find target genes From RNA seq data?
Cotton
DGE
RNA-seq
updated 14 days ago by
Ram
40k • written 14 days ago by
Fizzah
▴ 30
1
vote
2
replies
288
views
Dotplot error: subscript out of bound
R
scRNA-seq
seurat
9 days ago by
Xuhao
• 0
0
votes
2
replies
287
views
Gene Ontology - How to do Transcript Ontology?
gene-ontology
GO
transcripts
updated 22 days ago by
Ram
40k • written 24 days ago by
Lakritz the LabRat
• 0
0
votes
3
replies
287
views
QC of genetic data
PLINK
updated 7 hours ago by
bk11
★ 1.4k • written 4 days ago by
kl
▴ 10
0
votes
2
replies
287
views
STAR mapping two pass mode for multiple samples with split steps or one step ?
STAR
RNA-seq
22 days ago by
octpus616
▴ 90
1
vote
3
replies
286
views
differences between trajectories in conditions with Monocle3 or other tools
trajectory
scRna-seq
monocle3
single-cell
updated 4 days ago by
Amitm
★ 2.2k • written 4 days ago by
Chironex
▴ 40
0
votes
2
replies
285
views
Modify the code to take most abundant reads from a cluster and process it.
cd-hit-est
clustering
10 days ago by
Mo
▴ 40
0
votes
4
replies
285
views
Sequence quality drops in the 3' end of the left sequence in paired end. What could be the cause?
sequencing
updated 6 days ago by
GenoMax
135k • written 6 days ago by
eggrandio
▴ 40
0
votes
2
replies
284
views
circRNA isolation
circular-RNA
circRNA
cDNA
18 days ago by
dbagmerve
• 0
3
votes
3
replies
284
views
Clustering in single cell
seurat
single-cell
5 days ago by
Chris
▴ 200
2
votes
2
replies
284
views
Pangenome using Orthofinder
OrthoFinder
Pangenome
bacteria
15 days ago by
kirankumareripogu
▴ 10
1
vote
2
replies
284
views
Multiple testing adjustment for stepwise model selection
statistics
15 days ago by
mel22
▴ 100
0
votes
3
replies
284
views
Genomic location of MUC16
VNTR
genome
updated 26 days ago by
GenoMax
135k • written 28 days ago by
fi1d18
★ 4.2k
2
votes
4
replies
283
views
Integrated Seurat object change name of the two conditions
R
integrated
Seurat
8 days ago by
camillab.
▴ 140
0
votes
0
replies
282
views
GTF/Reference genome adjustments? Gene-based differential expression analysis of genetically modified mouse line
RNA-seq
DGE
updated 15 days ago by
Ram
40k • written 15 days ago by
alex
• 0
1
vote
3
replies
280
views
comparision of umap single cell
single-cell
5 days ago by
synat.keam
▴ 80
1
vote
1
reply
279
views
Which reference genome, DNA or cDNA and why?
or
DNA
cDNA
updated 29 days ago by
GenoMax
135k • written 4 weeks ago by
وفاء
• 0
0
votes
3
replies
275
views
Errors while trying to run Scenic
Scenic
updated 14 days ago by
GenoMax
135k • written 14 days ago by
Stavroula
• 0
0
votes
2
replies
275
views
Extract reference and alternate SNPs for different samples
vcftools
bcftools
SNPs
updated 26 days ago by
GenoMax
135k • written 26 days ago by
gubrins
▴ 270
1
vote
2
replies
270
views
Understanding TCGA barcodes with dot in the fieldname
tcga
updated 8 days ago by
Zhenyu Zhang
▴ 980 • written 11 days ago by
Tahsin
• 0
0
votes
3
replies
269
views
CNV using GISTIC
CNV
GISTIC
updated 20 days ago by
Ram
40k • written 20 days ago by
ananta.kapoor
• 0
0
votes
2
replies
269
views
Issues with featureCounts
featurecounts
rna-seq
differential-expression
updated 11 hours ago by
biofalconch
★ 1.0k • written 4 days ago by
Aime
• 0
327 results • Page
3 of 7
Recent Votes
Answer: tensorQTL interaction issue
Answer: Script for getting summary statistic of any genome using GTF or GFF3 ?
Comment: How do you validate and verify your pipeline's software updates?
Comment: How do you validate and verify your pipeline's software updates?
Answer: GSEA with GO and KEGG datasets
GSEA with GO and KEGG datasets
Answer: Exporting DEGs obtained from DESeq2
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Answer: Converting from BED to SAF/GFF
by
alejandrogzi
▴ 30
I recently developed [bed2gff](https://github.com/alejandrogzi/bed2gff) to quickly convert .bed files to a gff3 format, a tool written in R…
Comment: Convert bed12 to GFF
by
alejandrogzi
▴ 30
Now there is [bed2gff](https://github.com/alejandrogzi/bed2gff), if you want to work only with a gff file!
Comment: Converting different annotation file formats (GTF/GFF/BED) to each other
by
alejandrogzi
▴ 30
now there is also [bed2gff](https://github.com/alejandrogzi/bed2gff) if you want to convert .bed files to .gff3 files!
Answer: How Do I Convert From Bed Format To Gff Format?
by
alejandrogzi
▴ 30
I recently developed [bed2gff](https://github.com/alejandrogzi/bed2gff) to quickly convert .bed files to a gff3 format, a tool written in R…
Answer: tensorQTL interaction issue
by
Solal
• 0
Writing a solution for anyone interested. The issue stems from a dosage error, some variant contains only heterozygous individuals and no h…
Answer: The Biostar Handbook. A bioinformatics e-book for beginners.
by
Qboy
• 0
Great book! I love using it to help my mentees to learn bioinformatics better. It would be also fantastic to compile all other necessary Pr…
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
Thanks. I'll try.
Comment: How to get sample names and genotype for SNP in multi-sample VCF file
by
avelarbio46
▴ 30
Hi! I'm trying to parallelize your code (to run on a bunch of files), but it is not working because of multiple processes spawning. Do you …
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
GenoMax
135k
> so I don't think the genome size is available. Perhaps not for your specific strain but plenty of other Kpn genomes are available and th…
Comment: Kimura% of calcDivergenceFromAlign output
by
睿紘
• 0
For additional information, "Coverage for each repeat class and divergence (Kimura)" is also the result from calcDivergenceFromAlign.pl
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
By the way, I run Rockhopper on the untrimmed files, and the full match percentage grew to 20%-30%, and there are 1552 transcripts in the u…
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
Here are the total bases in 4 fastq files (after adapter removal): 1,027,852,411, 1,041,895,083, 1,121,786,520, 1,299,721,929. I just rem…
Comment: Understanding mother and father alleles in VCF file
by
c.
• 0
actually they didn't tell me which relatives were from mother side and which from father side, you are right. But how can you find related …
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
GenoMax
135k
Correct. Data that went into the assembly.
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
Total bases (number of reads * read length) should be calculated after removal of adapters, right?
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