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950 results • Page
2 of 19
Sort: Votes
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Views
Votes
Replies
3
votes
4
replies
1.4k
views
How to get regions with mapped reads
bed
bam
RNA-Seq
bedtools
updated 6 days ago by
Ram
38k • written 4.5 years ago by
nanoide
▴ 100
3
votes
4
replies
3.5k
views
How Can I Extract All The Read Pairs In A Region From Bam File ?
bam
updated 1 day ago by
Ram
38k • written 9.9 years ago by
siyu
▴ 150
3
votes
3
replies
337
views
Retrieving information from FASTA file
NGS
FASTA
VCF
updated 7 days ago by
Ram
38k • written 12 months ago by
Maryam
▴ 10
3
votes
1
reply
197
views
analysing nanopore amplicon data
amplicon
genotyping
nanopore
ampliSAS
updated 28 days ago by
dthorbur
▴ 420 • written 28 days ago by
khoojj
▴ 20
3
votes
6
replies
4.3k
views
Why Do I Get A Makeblastdb Error: File Does Not Match Input Format Type, Default Input Type Is Fasta
genomics
blast-plus
updated 27 days ago by
Ram
38k • written 9.3 years ago by
alok.helix
▴ 120
3
votes
4
replies
269
views
Creating sample groups from a combination of genes for survival analysis
Survival
7 days ago by
krushnach80
★ 1.1k
3
votes
2
replies
353
views
A way to find which genome builds a package supports
Bioconductor
genome
R
build
updated 17 days ago by
ATpoint
70k • written 18 days ago by
dshcherbo
• 0
3
votes
5
replies
1.9k
views
Cellranger count command for single end fastq
RNA-Seq
sequencing
updated 28 days ago by
Ram
38k • written 2.3 years ago by
sidrah.maryam
▴ 40
3
votes
1
reply
2.1k
views
reference and effective allele
snp
updated 19 days ago by
Ram
38k • written 8.3 years ago by
alex.crimi
• 0
3
votes
5
replies
1.7k
views
htseq counts to make matrix in Rstudio
RNA-Seq
updated 19 days ago by
Ram
38k • written 2.6 years ago by
simplysarah
• 0
3
votes
2
replies
246
views
KM Plot for gene of interest (e.g. TP53) using TCGA-PAAD dataset
PAAD
KM
Plot
TCGA
6 days ago by
Manav
• 0
3
votes
2
replies
506
views
[bam-readcount v.0.8.0] Fail to open BAM file –q
bam-readcount
BAM
updated 6 days ago by
Ram
38k • written 23 months ago by
vanessagpds
▴ 10
3
votes
6
replies
1.7k
views
Methods and type datastructures use by Splign?
data-structure
splign
updated 7 days ago by
Ram
38k • written 6.4 years ago by
Esaie
▴ 160
3
votes
4
replies
336
views
How much reads per cell for fusion detection with Nanopore ?
single-cell
fusion
nanopore
5 days ago by
Evan
▴ 180
3
votes
0
replies
1.8k
views
Combined VCF file for all GTEx genotype calls?
GTExv7
GTEx
Genotype
dbGap
VCF
updated 6 days ago by
Ram
38k • written 5.5 years ago by
django.waddle
▴ 60
2
votes
3
replies
347
views
HaplotypeCaller GENOTYPE GIVEN ALLELES doesn't genotype given alleles
Gatk
HaplotypeCaller
VCF
updated 6 days ago by
dthorbur
▴ 420 • written 5 weeks ago by
kamanovae
▴ 80
2
votes
2
replies
1.2k
views
How to obtain lineage information from taxid?
euitls
taxid2lineage
accession2taxid
updated 11 days ago by
Ram
38k • written 2.4 years ago by
6schulte
▴ 30
2
votes
2
replies
690
views
Adjusting data for covariates
R
updated 11 days ago by
Ram
38k • written 2.1 years ago by
chutt
▴ 20
2
votes
2
replies
3.3k
views
dyld : Library not loaded: Reason: image not found
python
dyld
R
updated 11 days ago by
Ram
38k • written 22 months ago by
jjp55
▴ 20
2
votes
10
replies
1.2k
views
multiple FASTA sequnces in to one full sequnce according to their chromosome number
NGS
FASTA
bam
updated 11 days ago by
Ram
38k • written 4.1 years ago by
Anu
• 0
2
votes
1
reply
596
views
Bioinformatics exercise on extracting FASTA sequences corresponding to taxon from a microbiome sequencing experimet in another file (.TSV)
fasta
homework
updated 11 days ago by
Ram
38k • written 2.2 years ago by
flisosergio
• 0
2
votes
0
replies
1.5k
views
Any relation between pan genome and genome plasticity?
genome
gene
sequence
updated 12 days ago by
Ram
38k • written 8.1 years ago by
Loub
▴ 20
2
votes
3
replies
420
views
Genomics regions that common to atleast 10 cell types
bedtools
bedops
genome
bed
sequence
updated 5 days ago by
Ram
38k • written 2.8 years ago by
rohitsatyam102
▴ 690
2
votes
4
replies
447
views
Trouble with transcripts_id when using how_are_we_stranded_here python package
bash
sequencing
RNA-seq
11 days ago by
ashleymb1116
• 0
2
votes
8
replies
531
views
Depth of Coverage in Mitochondrial Genome with Samtools
samtools
depth-of-coverage
Mitochondrial-genome
10 days ago by
Lida
• 0
2
votes
2
replies
315
views
Bioinformatics recursion practical applications
python
updated 21 days ago by
Ram
38k • written 6 weeks ago by
R.T. Canterbury
▴ 10
2
votes
3
replies
219
views
RNAseq: same reads + more complete scaffold = fewer DEGs?
RNAseq
updated 5 days ago by
Darked89
4.2k • written 7 days ago by
phillip.brassington
▴ 10
2
votes
3
replies
508
views
BWA generating a sam file without alignment information
sam
bwa
updated 7 days ago by
Ram
38k • written 13 months ago by
kelsey.e.witt
• 0
2
votes
2
replies
255
views
cant get FASTQ from basespace, only option is SAV
basespace
RNAseq
illumina
updated 18 days ago by
GenoMax
127k • written 18 days ago by
gkarabiyik
• 0
2
votes
0
replies
380
views
GWAS and QTL analysis bioinformatics tutorial from scratch
GWAS
pipeline
updated 19 days ago by
Ram
38k • written 8 months ago by
Dr.Animo
▴ 50
2
votes
8
replies
9.1k
views
Size of typical genomic data
genomic
updated 1 day ago by
Ram
38k • written 4.9 years ago by
Nicolas Rosewick
10k
2
votes
0
replies
2.0k
views
Kegg/Kaas Alternative Tool
kegg
updated 29 days ago by
Ram
38k • written 9.8 years ago by
anika.menon22
▴ 190
2
votes
10
replies
5.9k
views
fq.gz file use
BioEdit
R
updated 11 days ago by
Ram
38k • written 4.1 years ago by
choi.yisoo.hi
• 0
2
votes
2
replies
910
views
Supplier Mix Of Bioinformatics Service Providers
data-analysis
updated 29 days ago by
Ram
38k • written 9.7 years ago by
mathini.ilancheran
▴ 10
2
votes
5
replies
572
views
msigdbr returns the same genesets for mouse as in for human,
msigdbr
updated 7 days ago by
igor
13k • written 11 days ago by
e.r.zakiev
▴ 30
2
votes
2
replies
317
views
Rscript Nextflow Error: unable to load shared object
HPC
R
Nextflow
15 days ago by
Eliveri
▴ 340
2
votes
0
replies
490
views
3D distance between two chromosomal coordinates in 3D human genome?
3d
hi-C
genome
chia-pet
updated 5 days ago by
Ram
38k • written 2.0 years ago by
robert.mclaughlin
▴ 20
2
votes
4
replies
250
views
Error with a function in ATAC-seq analysis
ATAC-seq
5 days ago by
Chris
▴ 70
2
votes
12
replies
549
views
how to get to a VCF from bam files
Mpileup
Samtools
BAM
bcftools
VCF
updated 4 days ago by
Ram
38k • written 6 days ago by
Human
• 0
2
votes
2
replies
1.3k
views
Perl script problem: Remove duplicates from my fasta file with help of perl
perl
Fasta
updated 6 days ago by
Ram
38k • written 5.0 years ago by
ArusjakGevorgyan
▴ 20
2
votes
3
replies
2.4k
views
What is the Difference Between Within Gene Isoform Frequency and Global Isoform Frequency?
genomic
updated 25 days ago by
Ram
38k • written 9.1 years ago by
jack
▴ 500
2
votes
1
reply
196
views
is this good atac seq counts
ATAC-seq
updated 27 days ago by
Ram
38k • written 28 days ago by
alwayshope
• 0
2
votes
2
replies
79
views
STAR solo set parameters for scrna-seq
solo
STAR
2 hours ago by
t.ru
▴ 20
2
votes
1
reply
1.4k
views
Example data to verify of RNA-Seq Analysis pipeline
hisat2
RNA-Seq
pipeline
updated 14 days ago by
Ram
38k • written 5.2 years ago by
hkarakurt
▴ 150
2
votes
3
replies
1.9k
views
Does A Protein Interaction Network Created Directly From Literature Correspond To A Top Down Or A Bottom Up Approach?
network
model
updated 26 days ago by
Ram
38k • written 9.1 years ago by
Ashmit
• 0
2
votes
4
replies
586
views
Noob question: I want to use TCGA data to compare survival with mutations, how should I do it?
cancer
mutations
tcga
updated 11 days ago by
Ram
38k • written 10 months ago by
cdeantoneo31
▴ 10
2
votes
0
replies
1.2k
views
How To Screen Genomes For Compositional Studies?
genomics
updated 28 days ago by
Ram
38k • written 9.5 years ago by
SRKR
▴ 180
2
votes
3
replies
2.3k
views
Logic To Get Consensus Sequence
consensus
genomics
updated 28 days ago by
Ram
38k • written 9.4 years ago by
SRKR
▴ 180
2
votes
3
replies
333
views
FeatureCounts >edgeR > GO
RNA-SEQ
updated 8 days ago by
Basti
★ 1.4k • written 9 days ago by
Pegasus
▴ 80
2
votes
3
replies
277
views
mRNA Seq
NGS
updated 12 days ago by
Ram
38k • written 13 days ago by
Yaseen
▴ 10
950 results • Page
2 of 19
Recent Votes
Heatmap deseq2
A: Extract Base Based On Position From Bam File
PyComplexHeatmap: a Python package to visualize multimodal genomics data
pulling snps from vcf file using ids
A: pulling snps from vcf file using ids
Answer: Scaling RNA-Seq data before clustering?
Answer: How to use CAFE from Orthofinder Results
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Comment: Suggestion for approaching multiple conditions scRNA-seq
by
rpolicastro
11k
Do you have 2 biological or technical replicates for each condition? Also, you mentioned you didn't find dramatic changes with your analysi…
Comment: GO enrichment analysis
by
Antonio R. Franco
★ 5.0k
I had the same situation that I solved using the R package TopGO. You have already the list of GO terms. Need to follow the instructions i…
Comment: demultiplexing with guppy_basecaller and guppy_barcoder using --detect_mid_stran
by
andres.firrincieli
3.1k
Hi @6f8262bb I still have no idea what could be the cause but switching to guppy v6.2 solved this problem
Answer: Dealing with very large gene-lists in GSEA
by
Trivas
▴ 710
IMO, in those cases you could look at ES instead of NES. Regardless, within the function `fgsea`, you can set the parameters `minSize` and …
Comment: merge vcf.gz file
by
Mali
• 0
file1 = '/sci/labs/orzuk/orzuk/projects/Skin/data/Ichilov/vcfFiles/vcfFiles/eb3_1001_748-gatk-haplotype.final.vcf.gz' file2 = '/sci/labs/or…
Comment: Which GPU for local Colabfold
by
matt_arnold_bio
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Another giveaway is that when you hit run, it will say either "Running on GPU" or "Running on CPU" in the log output... ![enter image desc…
Answer: Which GPU for local Colabfold
by
matt_arnold_bio
• 0
Hi Arjen, This is a little while ago now so maybe this is not that helpful but... You should be able to tell if the GPU is being seen…
Comment: STAR solo set parameters for scrna-seq
by
t.ru
▴ 20
it's single cell mars-seq. Yes sorry its a fastq file.
Answer: boxplot issue
by
yura.grabovska
▴ 50
First of all, you're not outputting the result of your scale to anything... t(apply(mat, 1, scale)) colnames(mat) <- rownames(cold…
Comment: STAR solo set parameters for scrna-seq
by
GenoMax
127k
> a fasta file similar to this This is not a fasta file It is a fastq format file. Are you sure this is single cell RNAseq data? Looking…
Answer: heatmap issue
by
yura.grabovska
▴ 50
Your controls clearly show variable expression for the genes you're plotting so to get them to stick together on the left, you would need t…
Comment: How to demultiplex single end - dual index run - NextSeq 500
by
GenoMax
127k
Correct. This is a single-end dual indexed (8 bp each) run.
Comment: genome annotation [sstart] [send] - how to get protein sequence from gene
by
danfarkas
• 0
Hi shenwei356, Thanks for the clarification. However, I did try this, and it was not yielding the expected amino acid sequences. This is w…
Comment: Artificial reads - remove multiple mapped reads against reference genome, and on
by
Agamemnon
▴ 60
I ran ```samtools view -F 4 -q 42 test.bam | wc -l``` and I get ```6159141```, why is there a discrepancy, as originally only ```5200484```…
Comment: differential gene analysis
by
kalyani
• 0
okay thankks!
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