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1,000 results • Page
2 of 20
Sort: Rank
Rank
Views
Votes
Replies
2
votes
3
replies
135
views
Will flags in sam files be updated after filtering?
sam
alignment
samtools
4 days ago by
Zhang Yun
• 0
0
votes
0
replies
83
views
Need help in explanation about article's binary hypothesis
nipt
t-test
4 days ago by
khanhlpbao
• 0
0
votes
2
replies
166
views
Cellranger count pipestance failed: The read lengths are incompatible with all the chemistries
10x
cellranger
single-cell
4 days ago by
rmf
★ 1.4k
0
votes
0
replies
82
views
Can we get the results for Student t test and Welch t test by using limma?.
t-test
limma
4 days ago by
Minushi
• 0
0
votes
0
replies
73
views
Generating embeddings for antibody data
ML
fab
Alphafold
mab
antibody
4 days ago by
tom5
• 0
2
votes
2
replies
164
views
Snakemake MissingExceptionOutput
python
snakemake
RNA-seq
updated 4 days ago by
cpad0112
20k • written 4 days ago by
ohu20a
▴ 10
0
votes
0
replies
98
views
Cross-covariance LD matrix
LD
cross-covariance
4 days ago by
yasaman
• 0
0
votes
0
replies
75
views
ESTIMATION OF LEVEL OF CYTOKINES FROM BULK RNAseq
TCGA
CIBERSORT
TCELL
RNA-Seq
R
4 days ago by
leticia
▴ 20
0
votes
0
replies
90
views
How to extract the CDS from a fasta file using the protein IDS ?
gene
genome
protein
4 days ago by
sunnykevin97
▴ 760
0
votes
0
replies
85
views
Why do these samples have low UMI's and ngenes?
scrnaseq
seurat
single-cell
4 days ago by
crx6xw
• 0
0
votes
0
replies
79
views
How to set a seed for the k-clustering in my pheatmaps?
pheatmap
k-clustering
heatmap
kmeans
clustering
4 days ago by
Berta
• 0
0
votes
0
replies
80
views
How to use python packages for mixed model while differential testing?
DGE
mixed
model
lme4
MAST
5 days ago by
akilabioinfo
▴ 10
0
votes
0
replies
78
views
Is it possible to use RSEM with BAM files from STAR alignment?
RSEM
alignment
Pre-computed
STAR
5 days ago by
knowNot
• 0
0
votes
1
reply
93
views
Protein protein interaction network
cytoscape
DEGs
NDExintegratedquery
networkanalyst
updated 3 days ago by
scooter
▴ 560 • written 5 days ago by
iamsmor
• 0
0
votes
2
replies
136
views
Detecting duplicated UMI in BAM file
UMI
BAM
4 days ago by
tien
• 0
2
votes
0
replies
76
views
Signals of selection in RADseq with no population structure?
selection
RADseq
population-differentiation
outliers
5 days ago by
Natasha
▴ 10
0
votes
0
replies
70
views
Error in H5Fopen
atac-seq
scatac-seq
5 days ago by
bioinformatics.girl
• 0
0
votes
0
replies
71
views
Is a data fusion or a data integration done to bring together disparate metagenome datasets?
integration
data
metagenome
fusion
5 days ago by
seda
• 0
0
votes
3
replies
168
views
How to modify VCF file?
VCF
tabix
updated 4 days ago by
Pierre Lindenbaum
146k • written 5 days ago by
CHENG
• 0
0
votes
0
replies
75
views
AttributeError: module '__main__' has no attribute '/home/bvs/Neelam/resfinder/db_resfinder/'
Resfinder
updated 5 days ago by
GenoMax
117k • written 5 days ago by
Neel
▴ 10
0
votes
0
replies
72
views
Can we use logFC MLE for pathway analysis instead of logFC?
rnaseq
dge
5 days ago by
Tania
▴ 150
0
votes
3
replies
138
views
Pilon polishing crashes in beginning of second batch
pilon
5 days ago by
anitajnorman
• 0
0
votes
0
replies
70
views
PCA and rotations on Seqmonk
seqmonk
rotations
transformations
pca
5 days ago by
a_bis
▴ 30
0
votes
0
replies
79
views
Methylation calling -Primrose Package
Methylation
CIGAR
PacBio
5 days ago by
priya.bmg
▴ 20
0
votes
0
replies
64
views
Error in PCA
...
specified
not
oops
flag
-i
at
5 days ago by
hosin
• 0
0
votes
1
reply
116
views
error in connecting to R
R
shell
script
updated 5 days ago by
Matthias Zepper
▴ 910 • written 5 days ago by
parinv
▴ 50
0
votes
1
reply
1.6k
views
How to obtain .seq in BIC-seq2.1.1
BIC-seq
updated 5 days ago by
John Wick
• 0 • written 6.5 years ago by
seq-seek
• 0
0
votes
1
reply
139
views
BBduk reading fastq from S3 directly - Is it possibile?
BBduk
BBTools
BBMap
updated 5 days ago by
Mensur Dlakic
★ 19k • written 5 days ago by
Marko
• 0
2
votes
3
replies
168
views
How to find if a gene symbol is enriched in treatment vs control?
R
updated 4 days ago by
Friederike
8.4k • written 5 days ago by
coboyfan12
• 0
0
votes
0
replies
76
views
Errors when using psiblast
psiblast
blast
msa
5 days ago by
sknight5
• 0
0
votes
0
replies
73
views
Getting length of total region aligned to cluster of paired end reads
paired-reads
alignment
6 days ago by
arsala521
▴ 10
0
votes
2
replies
142
views
Comparisons between conditions without control
RNA-seq
updated 6 days ago by
Matthias Zepper
▴ 910 • written 6 days ago by
valentina.munoz.m
• 0
1
vote
4
replies
187
views
How to convert from csv to ped and map files?
plink
updated 6 days ago by
chrchang523
9.3k • written 6 days ago by
mahir.firefox
▴ 10
0
votes
3
replies
154
views
convert BGI stLFR reads to 10x format
10x
reads
stLFR
updated 6 days ago by
GenoMax
117k • written 6 days ago by
y.sevellec
• 0
3
votes
2
replies
145
views
Best Pipeline for De novo transcriptome SC-RNAseq
scRNAseq
bioinformatics
updated 4 days ago by
dsull
★ 3.1k • written 6 days ago by
Chris
▴ 10
0
votes
0
replies
100
views
Comparing FPKM/RPKM between two different datasets
FPKM
comparison
6 days ago by
Aleksandar
• 0
0
votes
0
replies
87
views
ValueError: The number of observations cannot be determined on an empty distance matrix.
CARD
6 days ago by
Neel
▴ 10
0
votes
5
replies
229
views
Create an Rstudio environment with the latest R version
R
environment
Rstudio
conda
updated 6 days ago by
Michael Dondrup
51k • written 6 days ago by
giammafer
▴ 20
0
votes
0
replies
73
views
Calculation of λ1000 for a continuous trait
SNP
GWAS
inflation
6 days ago by
Apprentice
▴ 100
0
votes
0
replies
91
views
Biologic Images processing: Immunofluorescences
microscope
image
immunofluorescence
developmental
processing
5 days ago by
Francesco
• 0
0
votes
1
reply
139
views
Identification of conserved and varaible regions on 16s rRNA sequences
16s
illumina
sequencing
NGS
updated 6 days ago by
Chris Dean
▴ 280 • written 6 days ago by
Robert
• 0
0
votes
0
replies
74
views
CHECKING PROPERIONAL OF SAMPLE IN GWAS CATALOGY
Catology
GWAS
6 days ago by
itsmestewartBM
• 0
0
votes
0
replies
66
views
MulticoreParam() not supported on Windows, use SnowParam()
r
xcell
rna-seq
bioconductor
tcga
6 days ago by
amirnavidinia2014
• 0
1
vote
2
replies
115
views
How to integrate multi omics data in cytoscape
cytoscape
protein-protein
interaction
network
2 days ago by
barrypraveen
▴ 30
0
votes
2
replies
152
views
0 enriched terms found (Gene Ontology Analysis)
ClusterProfiler
GeneOntology
6 days ago by
tsomakiank
• 0
2
votes
5
replies
289
views
how can we determin the length of first sequences in genome
genomic
sequence
6 days ago by
ikramb450
• 0
0
votes
0
replies
105
views
Triplicate (1 undetermined and 2 ct value) in qRT-PCR
triplicate
qRT-PCR
undetectable
undetermined
offtopic
updated 6 days ago by
ATpoint
62k • written 7 days ago by
YCW121
• 0
0
votes
0
replies
179
views
Unclear variation in level 5 LINCS gene perturbation Tx data and best practice to process
cmap
L1000
perturbation
transcriptomics
LINCS
6 days ago by
Yep
• 0
0
votes
3
replies
167
views
Plotting MAF summary
plotmafSummary
MAF
7 days ago by
Negatyvna
• 0
2
votes
3
replies
280
views
tophat2 -o tophat_fusion -p 15 --fusion-search --keep-fasta-order --bowtie1 --no-coverage-search hg19_bowtie1_index read_1.fastq read_2.fastq
circRNAs
Tophat2
ciriexplorer2
updated 7 days ago by
Pierre Lindenbaum
146k • written 7 days ago by
HaroonPakistan
• 0
1,000 results • Page
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Recent Votes
Answer: Exctract unmapped reads from BLAST
Answer: ESTIMATE tumor purity
C: Run PHI-BLAST in BLAST+ standalone
Answer: Junior bioinformatician in italy
Junior bioinformatician in italy
A: Thoughts on switching from Biology to Bioinformatics
The Biostar Handbook. A bioinformatics e-book for beginners.
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Recent Replies
Comment: Exctract unmapped reads from BLAST
by
Pilar
• 0
Thank you very much for your quickly response. The `comm` command has been very useful :) I figure I have the output file I needed.
Comment: Computing allele frequencies per individual for a set of SNPs in PLINK
by
chrchang523
9.3k
If 1kgtot.bim contains doubled IDs (e.g. "HG00096 HG00096", you need to provide a doubled ID to `--indv`. The log file shows that your scr…
Answer: ESTIMATE tumor purity
by
JACKY
▴ 10
Just in case anyone faces this problem in the future, here is the link for the updated `estimate package` on github: https://github.com/…
Answer: Exctract unmapped reads from BLAST
by
GenoMax
117k
> Is there a way to tell blast to output the unmapped reads into a > separate file? No there is not. You will need to use `-outfmt 7` whe…
Comment: Resfams Database usage
by
Neel
▴ 10
Hi, i have annotated genome by prokka and o want to search amr agaisnt resfams could you please tell me how to do it steps by steps.
Comment: Exctract unmapped reads from BLAST
by
Pierre Lindenbaum
146k
those are two distinct commands https://linux.die.net/man/1/comm and https://linux.die.net/man/1/join extract the names of your queries, s…
Comment: Exctract unmapped reads from BLAST
by
Pilar
• 0
Thanks for answering :), sorry, I'm new at this. These are commands I should use when I launch Blast in terminal?
Comment: Exctract unmapped reads from BLAST
by
Pierre Lindenbaum
146k
have a look at `comm` or `join`.
Comment: Computing allele frequencies per individual for a set of SNPs in PLINK
by
pifferdavide
▴ 100
Sorry I forgot to mention I used it in this loop, which worked with another plink file, but not with this 1KG file. for popfile in $(l…
Comment: Computing allele frequencies per individual for a set of SNPs in PLINK
by
chrchang523
9.3k
`--indv` takes a single ID, not a filename. So `--indv IDlist.txt` shouldn't work.
Answer: Quast Ouput
by
Ying
• 0
The "# contigs 47484" is based on contig of size >= 500 bp (see the first line of your screenshot). The "# contigs (>= 0 bp)" is the raw nu…
Answer: SRA obtained metagenomic reads appears to corrupt - cannot work on SingleM
by
Mensur Dlakic
★ 19k
Not sure why: 1) you are splitting reads; 2) you are fixing reads when they seem fine. I suggest something like this instead after `prefetc…
Comment: Quick way to get a field such as QNAME from the last read in a bam file?
by
kalavattam
▴ 70
Thank you. Do you know of any strategies for non-coordinated-sorted bam files?
Comment: analyzing a single cell and searching for a specific gene using single cell data
by
jared.andrews07
★ 13k
@rpolicastro gave you several helpful links for using the h5ad file, in addition to how to explore and plot data. The links in my answer al…
Comment: Computing allele frequencies per individual for a set of SNPs in PLINK
by
pifferdavide
▴ 100
I was able to run the --indv command successfully on a file with a bed file by simply listing the IDs in single column. However, with anoth…
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