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542 results • Page
2 of 11
Sort: Votes
Rank
Views
Votes
Replies
2
votes
6
replies
395
views
create a gene count matrix csv file from seurat object
Seurat
sparseMatrix
16 days ago by
jkim
▴ 170
2
votes
4
replies
300
views
Number of CPUs to use by DRAP for transcriptomic assmbly
DRAP
CPU
transcriptomics
26 days ago by
Mohamed Abderrahmane
▴ 20
2
votes
3
replies
3.0k
views
RNA-seq batch effect correction
RNA-Seq
batch-effect
sequencing
updated 6 days ago by
Ram
43k • written 6.9 years ago by
endikavarela
• 0
2
votes
1
reply
279
views
Forgot the web site with comparison of NGS platforms
NGS
platforms
updated 25 days ago by
cmdcolin
★ 3.8k • written 25 days ago by
konstantinkul
▴ 110
2
votes
6
replies
627
views
Overlapping and merging ChIP-seq peaks
MACS2
HOMER
ChIP-seq
peak-calling
updated 22 days ago by
Ram
43k • written 4 months ago by
zea
• 0
2
votes
1
reply
242
views
VEP annotation --per_gene allele choice
VEP
updated 12 days ago by
Ram
43k • written 12 days ago by
atariw
▴ 10
2
votes
7
replies
803
views
Removing duplicates
duplicates
ONT
minimap2
updated 5 days ago by
joe
▴ 510 • written 7 days ago by
quentinperriere
• 0
2
votes
4
replies
309
views
SnpEff annotates coding duplication as intronic?
snpeff
7 days ago by
kirill.zaslavsky
• 0
2
votes
18
replies
1.6k
views
error when running phg create-maf-vcf to build a built practical haplotype graph (v2)
pangenome
phg_v2
plants
PHG
22 days ago by
b.contreras.moreira
▴ 170
2
votes
9
replies
777
views
TCGA2STAT Error: Firehose connection
FIREHOSE
TCGA
updated 19 hours ago by
LauferVA
4.2k • written 6 months ago by
Gnana
• 0
2
votes
2
replies
270
views
DepMap: siRNA and CRSIPR discrepancy
DepMap
updated 13 days ago by
ATpoint
82k • written 13 days ago by
Shicheng Guo
★ 9.4k
2
votes
2
replies
1.6k
views
read count for RNA sequencing via nanopore
long-reads
RNA-seq
gene-expression
read-count
updated 19 days ago by
Ram
43k • written 2.9 years ago by
gat
• 0
2
votes
5
replies
427
views
Where to find the homopolymer regions bed file for Hg002 genome?
simple-repeats
hg002
bed
homopolymer-regions
7 days ago by
Bikram Kumar
• 0
2
votes
0
replies
202
views
What are good Python models/packages to predict small molecula (SMILES) binding to proteins ?
SMILES
9 days ago by
Alexander
▴ 220
2
votes
5
replies
673
views
what's the genomic location and name of gene responsible for orange and black patches in calico cats
genomes
updated 4 days ago by
Jesse
▴ 740 • written 5 days ago by
Ann
★ 2.4k
2
votes
10
replies
3.4k
views
RepeatMasker: createLib() Error
RepeatMasker
updated 7 days ago by
Ram
43k • written 7.4 years ago by
linda
• 0
2
votes
5
replies
668
views
samtools mpileup taking too long
Bam
samtools
sam
alignment
updated 19 days ago by
Ram
43k • written 6 weeks ago by
K
• 0
2
votes
3
replies
1.1k
views
Polishing PACBIO corrected assembly using ONT and ILLUMINA data (.fastq)
fasta
Nanopore
long-read
fastq
updated 19 days ago by
Ram
43k • written 2.8 years ago by
K
▴ 10
2
votes
10
replies
819
views
Only one read per run - Trying to use CellRangerv7
Fastq
CellRanger
updated 27 days ago by
Ram
43k • written 9 weeks ago by
Sky
▴ 10
2
votes
3
replies
1.5k
views
Duplicate row name not allowed while reading the file in R
RNA-Seq
deseq2
Differential-Gene-expression
R
updated 15 days ago by
Ram
43k • written 4.9 years ago by
imanbh
• 0
1
vote
3
replies
1.7k
views
Using Nanoplot to asses quality of ONT data
ONT
Long-read-sequencing
NanoPlot
updated 19 days ago by
Ram
43k • written 2.3 years ago by
matt81rd
▴ 10
1
vote
1
reply
409
views
Difference when using difference number of pathways score to compare between 3 groups
GSVA
Limma
updated 3 days ago by
Gordon Smyth
★ 7.0k • written 6 days ago by
Chris
▴ 260
1
vote
0
replies
535
views
The majority of the sequences in Trinity ID do not start from start codons. Can the sequences still be used for primer design and validation?
Primers
TRINITY
4 days ago by
mathavanbioinfo
▴ 90
1
vote
1
reply
456
views
PLINK multivariable GLM output: p-value without BETA and SE
linear-model
glm
plink
updated 16 days ago by
chrchang523
10k • written 16 days ago by
dsbusiness135
▴ 10
1
vote
4
replies
806
views
I want to calculate if the sample has whole genome duplication event or not?
sequencing
wgs
14 days ago by
Hyper_Odin
▴ 310
1
vote
1
reply
173
views
Help/Direction Needed for Data Analysis of RNA
Sequencing
RNA-seq
DNA-Seq
updated 8 days ago by
dsull
★ 5.8k • written 8 days ago by
niruf
• 0
1
vote
3
replies
224
views
some error in building kraken2 database
metagenome
kraken2
18 hours ago by
Art1ess
• 0
1
vote
4
replies
2.4k
views
Batch effect control in GWAS data.
Batch-effect
omniexpress
GWAS
GSA
updated 7 days ago by
Ram
43k • written 4.8 years ago by
saksis.rihards
▴ 10
1
vote
1
reply
190
views
Tool for gene function identification
Gene-Function
updated 22 days ago by
Ram
43k • written 22 days ago by
Estevão
• 0
1
vote
1
reply
179
views
Do I need to go back and filter my long-reads?
alignment
nanopore
filtering
QC
ONT
updated 9 days ago by
GenoMax
141k • written 9 days ago by
eesiribloom
▴ 80
1
vote
2
replies
268
views
Can I readmap short reads to rDNA references?
rDNA
assembly
updated 7 days ago by
Ram
43k • written 7 days ago by
aniigodwinn
• 0
1
vote
1
reply
172
views
Failed to download data from EBI with ascp
EBI
aspera
updated 13 days ago by
GenoMax
141k • written 13 days ago by
biock
▴ 60
1
vote
1
reply
472
views
Manta: What does all this mean
manta
Variant-calling
VEP
updated 22 days ago by
Ram
43k • written 9 months ago by
josh.mannheimer
• 0
1
vote
0
replies
366
views
Differential expression analysis on multiple integrated datasets
microarray
Z-score
limma
differential-gene-expression
meta-analysis
updated 15 days ago by
Ram
43k • written 2.4 years ago by
michael.s
▴ 10
1
vote
2
replies
282
views
Tumour purity and ploidy estimation
Copy-number
Whole-exome-sequencing
8 days ago by
bp22
▴ 80
1
vote
3
replies
251
views
CCA with random effects model
CCA
updated 22 days ago by
dariober
14k • written 22 days ago by
leranwangcs
▴ 120
1
vote
1
reply
193
views
ComplexHeatmap anno_mark position changes with height of gene expression heatmap
ComplexHeatmap
9 days ago by
CTLong
▴ 110
1
vote
2
replies
820
views
Replacing default numbers with geneNames in enhancedVolcano
EnhancedVolcano
updated 22 days ago by
Ram
43k • written 2.6 years ago by
Mohan
• 0
1
vote
0
replies
1.0k
views
interpretation of trajectory and batch effect
monocle3
batch-effect
trajectory
updated 16 days ago by
Ram
43k • written 3.5 years ago by
tushard1987
▴ 10
1
vote
2
replies
200
views
In need of help with my RNA velocity trajectory inference pipeline!
RNAvelocity
16 days ago by
phhelou5
• 0
1
vote
1
reply
150
views
Failed kmer content
kmer
illumina
ngs
updated 1 day ago by
Ram
43k • written 1 day ago by
Kasturi
• 0
1
vote
0
replies
154
views
DESeq2 comparing timepoints within treatment and account for background control change
RNAseq
DEG
DESeq2
updated 13 days ago by
Ram
43k • written 13 days ago by
ian.will
▴ 30
1
vote
2
replies
262
views
Can I compare kallisto counts from samples with different amount of reads?
RNA-seq
kallisto
7 days ago by
bioinfo
▴ 150
1
vote
3
replies
339
views
Ensembl gene id conversion
biomart
ensembl
updated 8 days ago by
ATpoint
82k • written 10 days ago by
naveedhasan2000
• 0
1
vote
2
replies
323
views
BAM to CRAM and BAM recover with smaller size
BAM
samtools
CRAM
updated 21 days ago by
jkbonfield
★ 1.2k • written 5 weeks ago by
geocarvalho
▴ 360
1
vote
1
reply
222
views
Retrieve a % coverage for each transcript
RNA-seq
updated 7 days ago by
Ram
43k • written 7 days ago by
jammydodger123456
▴ 40
1
vote
7
replies
886
views
GATK Mutect2 Input files reference and features have incompatible contigs: No overlapping contigs found.
hg19
Mutect2
GATK
Variant-Calling
updated 29 days ago by
Ram
43k • written 9 months ago by
Manuel Sokolov Ravasqueira
▴ 100
1
vote
0
replies
147
views
Contradictions in GSEA results
GSEA
Enrichment-analysis
PEA
updated 27 days ago by
Ram
43k • written 27 days ago by
Tolga
▴ 30
1
vote
0
replies
670
views
Conditions for valid batch effect correction
r
batch-effect
updated 7 days ago by
Ram
43k • written 5.2 years ago by
Sebastian Hesse
▴ 340
1
vote
0
replies
1.8k
views
Using SVA with RRBS data
sva
batch-effect
RRBS
updated 7 days ago by
Ram
43k • written 7.1 years ago by
mrodrigues.fernanda
▴ 50
542 results • Page
2 of 11
Recent Votes
Comment: downloading genomes in fasta format from accession ids
Comment: PCA plot (Suggestions Needed)
Answer: PCA plot (Suggestions Needed)
PCA plot (Suggestions Needed)
Comment: To get p-values for the TPM
A: Where To Download Pam50 Gene Set?
A: Where To Download Pam50 Gene Set?
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Comment: Adding CB tag to bam file
by
swbarnes2
14k
I think both these programs are just adding the same tag to every line. I don't think either cares whether it's called RG or CB
Comment: PCA plot (Suggestions Needed)
by
swbarnes2
14k
Even if this was real data, the tiny numbers for the PC1 and PC2 indicate that the differences between the samples are all absolutely tiny.…
Comment: Creating Synthetic Sequences for a ML Model
by
biochugs
• 0
Thanks for the input. I'll continue to think of more ideas that may be achievable.
Comment: PCA plot (Suggestions Needed)
by
Jeremy
▴ 890
You could also try kernel PCA, which is a non-linear form of PCA, although this will be more time-consuming.
Comment: Troubleshooting RNA-seq data with DNA contamination
by
DKA
▴ 40
I have unstranded RNA-seq data. Please share the tool with me, if you do not mind.
Comment: PCA plot (Suggestions Needed)
by
Mensur Dlakic
★ 27k
Everything that @f1f58aed said, plus that there may be no data manipulation that will give you the clusters you expected. Sometimes there a…
Answer: Creating Synthetic Sequences for a ML Model
by
Mensur Dlakic
★ 27k
It is a good idea, though I don't see how it would work in practice. I think you will encounter at least additional problem on top of da…
Answer: PCA plot (Suggestions Needed)
by
Jeremy
▴ 890
Right now, the WT samples are clustering together, but you have one mutant sample off by itself, which could be an outlier. How did you sc…
Comment: Adding CB tag to bam file
by
GenoMax
141k
@yokofakun had written this: https://www.biostars.org/p/336965/ https://github.com/ruqianl/appendCB may also be useful.
Comment: Troubleshooting RNA-seq data with DNA contamination
by
GenoMax
141k
Please post information about your tool as a `tools` category post. Don't wait/ask other users to ask you.
Comment: Adding CB tag to bam file
by
Maria
• 0
Sorry for the delay in my answer, I got sidetracked. I am using ```samtools addreplacerg -r "@RG\tID:$cell" -o $cell.bam -h $LINE``` bu…
Answer: Troubleshooting RNA-seq data with DNA contamination
by
haibol2017
▴ 10
We just developed a R package to tackle this problem. Do you have stranded or unstranded RNA-seq data contaminated with gDNA? If you wou…
Comment: How to trim transcripts using information from NCBI contamination screen report
by
GenoMax
141k
`bbduk.sh` can use the transcripts as input. There is a `adapters.fa` included in the `resources` directory of the distribution. You can do…
Comment: PCA plot (Suggestions Needed)
by
swbarnes2
14k
Cross posted here (including the code used to make up the counts): https://support.bioconductor.org/p/9157911/#9157959
Comment: To get p-values for the TPM
by
swbarnes2
14k
RSEM returns a kind of p-value if you run it with the "prior-enhanced" option. Did you do that? Are you completely sure that's the value y…
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