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1,000 results • Page
3 of 20
Sort: Votes
Rank
Views
Votes
Replies
10
votes
15
replies
6.1k
views
DIAMOND blast imported into MEGAN6 has no taxonomic assignment
blast
MEGAN6
Taxonomy
DIAMOND
7.4 years ago by
Farbod
★ 3.4k
10
votes
8
replies
2.3k
views
Blast scores...two annotations for the same piece of sequence
alignment
updated 22 months ago by
Ram
43k • written 9.1 years ago by
friasoler
▴ 50
10
votes
5
replies
5.5k
views
Intron Retention And Alternative 5'3' Splice Site Identification
updated 10.2 years ago by
Biostar
20 • written 13.0 years ago by
Arun
2.4k
10
votes
10
replies
3.8k
views
Parameter optimization STAR
RNA-Seq
6.2 years ago by
XBria
▴ 90
10
votes
2
replies
596
views
any script that can do this task
shell-script
updated 2.1 years ago by
Ram
43k • written 2.1 years ago by
Confused_human
▴ 20
10
votes
6
replies
8.3k
views
Any idea how to solve this error in fgsea - "Error in if (any(simpleFgseaRes$modeFraction < 10)) { : missing value where TRUE/FALSE needed"?
fgsea
updated 2.8 years ago by
alserg
▴ 920 • written 2.8 years ago by
gabrielbaldanzi
▴ 10
10
votes
23
replies
2.4k
views
Two simillarly annotated sequence has no alignment similarity. Why?
blast
alignment
gene
updated 6.4 years ago by
GenoMax
141k • written 6.4 years ago by
Farbod
★ 3.4k
10
votes
12
replies
2.0k
views
The 2013 Eisenberg and Levanon housekeeping genes list for Human is the most updated one?
genes
human
housekeeping
list
5.2 years ago by
msimmer92
▴ 300
10
votes
10
replies
2.0k
views
Minor allele frequency
GWAS
SNPs
Alleles
updated 5.4 years ago by
Biostar
20 • written 7.4 years ago by
alessandrotestori7
▴ 420
10
votes
1
reply
3.5k
views
RNA-SeQC, EVER-seq, RSeQC and CollectRnaSeqMetrics, which to use and *why*?
RNA-Seq
qc
updated 20 months ago by
Ram
43k • written 8.9 years ago by
Niek De Klein
★ 2.6k
10
votes
7
replies
1.2k
views
Can two mates have different file size?
sequencing
fastq
5.7 years ago by
marongiu.luigi
▴ 710
10
votes
13
replies
5.8k
views
How to identify DE lncRNA from RNA Seq Data?
RNA-Seq
lncrna
7.1 years ago by
Vasu
▴ 770
9
votes
13
replies
3.4k
views
Python Script to map reads to reference sequence
sequence
python
mapping
script
reference sequence
5.0 years ago by
Fid_o
▴ 40
9
votes
5
replies
1.2k
views
how to retrieve all proteins related to cancer
proteins
7.9 years ago by
Learner
▴ 280
9
votes
7
replies
3.3k
views
Aligning Miseq With Deletions >10 Bases
alignment
miseq
next-gen
updated 10.7 years ago by
Biostar
20 • written 11.3 years ago by
Leszek
4.2k
9
votes
13
replies
1.6k
views
SNP's and Gene?
SNP
gene
4.5 years ago by
imgapgenomika
▴ 10
9
votes
11
replies
1.5k
views
6 follow
Split up excel cell into multiple records
excel
byonic
6.8 years ago by
rshipman
▴ 30
9
votes
10
replies
2.8k
views
About GATK4 Mutect2 runtime (Whole Exome seq)
GATK
Whole Exome Sequencing
Mutect2
3.2 years ago by
kwanghoon
▴ 20
9
votes
5
replies
3.9k
views
What's the interpretation behind Pathway enrichment analysis
networks
pathway enrichment analysis
gene
updated 6.6 years ago by
Biostar
20 • written 6.8 years ago by
Chaimaa
▴ 260
9
votes
16
replies
1.9k
views
feature extraction from SNPs in R
R
gene
sequence
SNP
5.4 years ago by
bioinfo456
▴ 150
9
votes
19
replies
2.3k
views
There are any way to use a script for feed emboss with fasta sequence?
sequence
4.2 years ago by
schlogl
▴ 160
9
votes
10
replies
2.2k
views
[solved] Convert fold changes of multiple transcripts of a given gene to a single value characterising this gene
RNA-Seq
7.6 years ago by
biostart
▴ 370
9
votes
10
replies
3.4k
views
generation of heat map by excel data.
R
excel
heatmap
updated 5.8 years ago by
Ram
43k • written 5.8 years ago by
abhilashreddy495
▴ 10
9
votes
6
replies
1.3k
views
Genotype meaning
genotype
BL21
2.8 years ago by
A_heath
▴ 160
9
votes
10
replies
1.8k
views
How to use Poisson distribution to get meaningful expression counts?
statistics
rna-seq
updated 2.8 years ago by
i.sudbery
19k • written 2.8 years ago by
c_u
▴ 520
9
votes
15
replies
1.3k
views
Construction of single sequence assembly out of contigs
Contigs
Bacteria
Genome
WGS
4 months ago by
analyst
▴ 20
9
votes
12
replies
1.4k
views
Copy number variations analysis
R
CNV
updated 4.4 years ago by
zx8754
11k • written 4.4 years ago by
rprog008
▴ 70
9
votes
7
replies
1.2k
views
Reference genome location
reference
positions
FASTA
alignment
genome
updated 15 months ago by
barslmn
★ 2.1k • written 15 months ago by
Batel
• 0
9
votes
6
replies
3.4k
views
SOAPaligner 2.21 - does it replace all Ns by Gs in reads?
Soapaligner
alignment
updated 2.5 years ago by
Ram
43k • written 9.9 years ago by
Philipp Bayer
8.3k
9
votes
7
replies
2.4k
views
Merging BWT indices for BWA
BWA
BWT
read aligner
merge
5.5 years ago by
rgc255
▴ 60
9
votes
4
replies
2.1k
views
[Code] Converte fasta/fa files to fastq
perl
fastq
sed
fasta
updated 20 months ago by
Ram
43k • written 8.4 years ago by
Shicheng Guo
★ 9.4k
9
votes
7
replies
5.5k
views
fisher test with multiple samples
statistics
updated 2.4 years ago by
Ram
43k • written 9.8 years ago by
juncheng
▴ 220
9
votes
7
replies
1.1k
views
ChIP-seq; investigate binding within region of tRNA genes
tRNA
ChIP
nf-core
ChIP-seq
updated 23 months ago by
Ram
43k • written 24 months ago by
2138493o
▴ 20
9
votes
8
replies
1.7k
views
Union of unaligned fastq reads
RNA-Seq
6.8 years ago by
Jeffin Rockey
★ 1.3k
9
votes
13
replies
15k
views
GC Content of Fasta file --- Python Help
sequence
7.2 years ago by
Patrick Brennan
▴ 50
9
votes
1
reply
6.1k
views
Why does MACS2 report multiple records for the same peak region?
ChIP-Seq
macs
macs2
updated 20 months ago by
Ram
43k • written 8.5 years ago by
James Ashmore
★ 3.4k
9
votes
5
replies
711
views
Prophage prediction tool
prophage
PHASTER
prediction
updated 9 months ago by
GenoMax
141k • written 9 months ago by
A_heath
▴ 160
9
votes
13
replies
2.4k
views
single tumor vs multiple normal sample differential gene expression (RNA-Seq ) analysis using DESeq2
RNA-Seq
DESeq2
updated 3.0 years ago by
Ram
43k • written 3.0 years ago by
sumitguptabt
▴ 30
9
votes
27
replies
7.5k
views
How do I use Glimmer 3.02?
sequencing
updated 5.2 years ago by
ojelizodun
• 0 • written 6.1 years ago by
nattzy94
▴ 50
9
votes
3
replies
7.2k
views
Statistical Distributions In Rna-Seq Data Analysis
statistics
rna
updated 5.1 years ago by
Biostar
20 • written 12.1 years ago by
Ngsnewbie
▴ 380
9
votes
13
replies
2.4k
views
Reannotating a gene: Identifying translation and transcription start sites
RNA-Seq
genome
gene
7.3 years ago by
rh5118
▴ 40
9
votes
7
replies
2.1k
views
Sorting BLAST output files together?
blast
8.0 years ago by
zgayk
▴ 90
9
votes
13
replies
2.8k
views
qRT-PCR data analysis steps and workflow
qRT-PCR
Reference genes
Normalization
DeltaCt
FC
5.2 years ago by
mohammedtoufiq91
▴ 250
9
votes
11
replies
6.6k
views
microRNA Illumina Sequencing - Very low alignment rate
sequencing
Assembly
RNA-Seq
updated 2.2 years ago by
Ram
43k • written 9.7 years ago by
wynstep
▴ 90
9
votes
7
replies
5.3k
views
How to combine a different sources of pathway database (KEGG, BioCarta) for Pathway analysis.
Pathway
enrichment analysis
GWAS
pathway analysis
7.8 years ago by
kmsh410
▴ 40
9
votes
14
replies
4.3k
views
Explanation on Paired end single index for RNA seq
RNA-Seq
updated 2.0 years ago by
Ram
43k • written 9.2 years ago by
hothriananya
▴ 70
9
votes
2
replies
572
views
Answer needed urgently
Deep
updated 19 months ago by
Mensur Dlakic
★ 27k • written 19 months ago by
Deepak
• 0
9
votes
2
replies
3.1k
views
GEOquery Problems to Get GEO dataset
Geoquery
8.1 years ago by
Shicheng Guo
★ 9.4k
9
votes
16
replies
3.8k
views
6 follow
covert SAM to full length fasta
fasta
SAM
updated 13 months ago by
Ram
43k • written 5.7 years ago by
marongiu.luigi
▴ 710
9
votes
12
replies
7.3k
views
How to align reads on reference using python?
genome
sequence
gene
sequencing
updated 5.4 years ago by
WouterDeCoster
47k • written 5.4 years ago by
doramora
▴ 10
1,000 results • Page
3 of 20
Recent Votes
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43k
Your tutorial required `sudo` and thus is not doable for a majority of people. Any alternative to `sudo snap`?
Comment: Should I apply doublet detection for each pool seperately?
by
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Do not delete posts that have received feedback,
Comment: QC exclusion of A/T or G/C alleles to avoid strand issues
by
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▴ 10
They aren't merging any datasets. Just for reference the paper is this: https://www.nature.com/articles/s41380-022-01674-9#MOESM1. Citing: …
Comment: RNA seq differential expression analysis
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★ 1.7k
https://help.galaxyproject.org/
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The tool RSEM, which performs transcript quantification for RNA-Seq data, provides FPKM.
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by
Enrique
• 0
Hi! I'm still interested in the step that you use for the SNP calling. Did you use `trimmomatic` or another tool for trimming before mappin…
Comment: Could you please assist in identifying this cluster?
by
ATpoint
81k
The information do not suffice. Please show plots and identified marker genes. Also, make sure it is not a trash cluster with low number of…
Comment: Free/open source 23andme-like analysis
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▴ 510
Thank you, I added 'accurate' now ;) I'm surprised to find out that there isn't some tool like multiqc that just summarizes all this infor…
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81k
Guys, just use `samtools sort` which won't get you into trouble with any of that.
Comment: Ciriquant not configuring hisat2 indexed files
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Carlo Yague
8.6k
I don't know for sure, but I think that decreasing the amount of threads (1 or 2 instead of 4) will decrease the memory requirements of the…
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For me, setting `TMP_DIR` (`--TMP_DIR /home/tmp`) didn't work in picard 2.21.1. What solved the issue was setting the environmental variabl…
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by
Dinmukhamed
• 0
I found answer https://www.biostars.org/p/101191/.
Comment: Ciriquant not configuring hisat2 indexed files
by
Atul K.
• 0
it worked. Thank you. Now my terminal is crashing, seems 16gb of RAM and 4 cores isn't enough. Can you suggest something?
Comment: Adding CB tag to bam file
by
Maria
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I checked Picard ```AddOrReplaceReadsGroups``` and ```AddOATag```. Is that what you're referring to?
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Noted that 'accurate' wasn't among your criteria ;) Anyway, most variant callers are free open-source software. However, for ancestry ana…
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