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Recent Replies
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C: Is there any way to merge intervals based on a minimum overlap? by
VicGB • 0
Thanks! I will give it a try and reply back as soon as possible about the results.
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C: Code for splitting long string into Genbank record gives error by
toth.joe • 10
Thanks for clarifying the source code. I entered the format as you suggest but still get an erro...
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A: fastq-dump: failure to get two fastq files by
ATpoint ♦ 13k
When checking on [SRA][1] the first read is indeed listed with read length 0. I would guess that ...
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C: Tools for Multi-omics analysis (RNA-Seq, Metagenomics and Metatranscriptomics da by
mohammedtoufiq91 • 20
Yes, each datasets would be analyzed individually. For instance, differentially expressed genes w...
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C: Generate Consensus sequence from BAM file by
fatimarasool135 • 0
Hi sohail, I have 6 wheat samples sequenced using RNA-seq. I received forward and reverse fastq f...
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C: extracting the soft clipped seq only from a sam file by
SDin • 0
Hi, IrK, sorry for bothering you. Have you solved this problem? It will be very appreciated that ...
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C: Problem in converting vcf to maf using vcf2maf tools by
Kevin Blighe ♦ 37k
What h.mon means is that, if you have VEP installed at `/Raheleh/programs/vep/`, then you should ...
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A: HLA typing for cancers/neoantigen identification by
Kevin Blighe ♦ 37k
In order to understand what has changed in HLA in the tumour cells, one has to know the HLA genot...
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A: Peak caller for broad peaks, replicates and paired-end reads by
boczniak767 • 620
You could try [Homer][1], the first step is creating Tag directory, which allow to use paired-end...
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C: Are small RNAs included in a typical Illumina RNA seq dataset? by
ATpoint ♦ 13k
Unlikely to have smallRNAs in it. First you need a special RNA extraction kit to capture the smal...
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A: Cytoscape - disproportionately big files by
Jean-Karim Heriche ♦ 18k
Most likely this has to do with compression. Compression doesn't always reduce file size. Compres...
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C: Typical cutoffs for hyper- and hypo-methylation? by
ATpoint ♦ 13k
No, I recommend against this strategy for the following reason: NGS-based experiments always suff...
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