Tutorial:
Sequence Alignment from Scratch with BWA
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thank you, I was able to get the coordinates of the gene, but I need to extract the TSS and extend it to 1000 bp.
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I'm not going to give you a complete answer, but you can start looking at this posts: https://www.biostars.org/p/147351/
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Do not post screenshots: instead use some lines of input and the desired output.
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Thank you very much for your response.
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removeBatchEffect() wouldn't produce the outlier effect that you show on the right unless there was something seriously wrong with the batc…
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R codes：
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####数据获取####
#引文
#citation(package = "GEOquery")
#数据提取
GSE53625_matrix<-GEOquery::getGEO(GEO = "GSE5362…
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It is a great honor to receive your response.
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Great answer, narrowing it down the the key points!
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The righthand plot might be totally fine once you remove that one outlier on the left, try that first. The batch effect manifesting along P…
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