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Recent Replies
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C: hisat2: "(ERR): hisat2-align died with signal 6 (ABRT) "-message by
genomax ♦ 91k
If you provide two different sets of reads with `-1` and `-2` option the software thinks that the...
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C: How to run DESeq2 for multiple factors by
dsull • 1.6k
Can you give us more information? Is your script working or is it producing an error? Which spec...
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C: Quallity trimming by phred score, how far is to far? by
robert.murphy • 20
Ahh okay so that is normal, I was not aware that was the case. thank you! I just assume it binned...
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C: hisat2: "(ERR): hisat2-align died with signal 6 (ABRT) "-message by
Ad fontes • 140
Sorry, I don't think this is the answer: I am trying to match two sets of reads against the refe...
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C: Quallity trimming by phred score, how far is to far? by
genomax ♦ 91k
How so? Before filtering all of your reads were 150 bp (FastQC just plots them like that). After ...
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C: Downloading HLA typing for human by
A • 3.9k
Sorry @ATpoint I have found files of some HLA type named mhcflurry_alleles.txt , netMHCII_...
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C: Quallity trimming by phred score, how far is to far? by
robert.murphy • 20
Thank you very much. I have just tried a Q30 filtering run and get back some quite weird results ...
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C: hisat2: "(ERR): hisat2-align died with signal 6 (ABRT) "-message by
Ad fontes • 140
According to https://daehwankimlab.github.io/hisat2/manual/ we should use --rna-strandness F (o...
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C: Does trimming RNA-Seq reads affect the differentially expressed genes? by
benformatics • 2.0k
When you say transcripts... what do you mean exactly? After aligning the newly trimmed reads the ...
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C: hisat2: "(ERR): hisat2-align died with signal 6 (ABRT) "-message by
genomax ♦ 91k
Why are you using the option for paired-end reads if you have single-end data?
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A: hisat2: "(ERR): hisat2-align died with signal 6 (ABRT) "-message by
genomax ♦ 91k
Looks like you don't have the same number of reads in Read 1 and Read 2 file. This can happen if ...
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A: hisat2: "(ERR): hisat2-align died with signal 6 (ABRT) "-message by
Ad fontes • 140
It is single-read, but even when I specify rna-strandness R I get the same answer. Any help wo...
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