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Recent Replies
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A: Can someone explain what the -M option in BWA-MEM does? by
Devon Ryan ♦ 80k
"Split hits" here means chimeric alignments, otherwise known as "supplementary alignments", such ...
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C: How to improve single-cells data ? by
darbinator • 0
Bacterial genomes (is that why I chose to conserve metagenome contigs that align over 95% of my S...
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C: plasmid assemby from Illumina reads - help! by
rebeccarenberg • 0
target=100 was too high. I dropped it down to 20/30/50 and I got a beautiful assembly. THANK YOU!...
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A: Best way to systematically select ENCODE data to download? by
Eric Lim • 580
I am primarily interested in their KD and control RNA-Seq, so I ended up writing a couple simple ...
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C: plasmid assemby from Illumina reads - help! by
rebeccarenberg • 0
Yes, the 1x150 sequencing is accurate. Thank you for the suggestions. I normalized using b...
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C: Retrieve PubMed records based on some genes by
genomax ♦ 49k
I am going to make some general comments. You will want to use `OR` instead of `AND` in your ter...
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C: How to improve single-cells data ? by
Friederike • 1.8k
what DNA did you sequence? mammalian? bacterial?
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C: plasmid assemby from Illumina reads - help! by
genomax ♦ 49k
> 1x150 Is that accurate? Or was it a paired-end run and was actually `2x150`? You may want ...
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A: Where to find Drosophila melanogaster de novo transcript assembly data? by
jaudall • 0
It would still be useful ... coincidently I'm looking for it too to test a genome annotation pipe...
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A: CWL passing secondary files in a workflow by
biokcb • 110
It looks like part of the problem here is that you are trying to use an input `bai` that doesn't ...
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C: Total CpG genome count by
Vijay Lakhujani ♦ 2.5k
Hello [rebekah_321][1], Don't forget to follow up on your threads. If an answer was helpful, y...
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A: RSEM build for C. elegans by
Michele Busby • 1.8k
I figured it out but I'll leave it here if anyone else runs into this: RSEM expects a ; after th...
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